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1. 发明授权

US06943279B1 Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use 失效
标题翻译：氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法
公开(公告)号：US06943279B1
公开(公告)日：2005-09-13
申请号：US09658835
申请日：2000-09-08
申请人： Jonathan P. Duvick , Jacob T. Gilliam , Joyce R. Maddox , Aragula Gururaj Rao , Oswald R. Crasta , Otto Folkerts
发明人： Jonathan P. Duvick , Jacob T. Gilliam , Joyce R. Maddox , Aragula Gururaj Rao , Oswald R. Crasta , Otto Folkerts
IPC分类号： C12N9/06 , C12N15/09 , C12N15/31 , C12N15/82 , A10H1/00 , A10H5/10
CPC分类号： C12N9/0022 , C12N15/8242 , C12N15/8282
摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera and Rhinocladiella airovirens. The polynucleotides may be mutated to remove glycosylation sites and cysteine residues. Additionally, the present invention provides recombinant expression cassettes, host cells, transgenic plants, and transgenic seed. The present invention also provides for polynucleotides containing both APAO and a fumonisin esterase. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and eukaryotic systems, methods for detecting fumonisins, and methods for identifying transformed plant cells. Methods for degrading fungal toxins in plants, grain, grain processing, silage, food crops and in animal feed are also disclosed.
摘要翻译：本发明提供了从Exophiala spinifera和Rhinocladiella airovirens分离的酶APAO的多核苷酸和相关多肽。可以突变多核苷酸以除去糖基化位点和半胱氨酸残基。另外，本发明提供了重组表达盒，宿主细胞，转基因植物和转基因种子。本发明还提供了含有APAO和伏马菌素酯酶的多核苷酸。此外，本发明提供了在原核和真核系统中生产APAO酶的方法，用于检测伏马毒素的方法和用于鉴定转化的植物细胞的方法。还公开了降解植物，谷物，谷物加工，青贮饲料，粮食作物和动物饲料中真菌毒素的方法。

2. 发明授权

US06670189B2 Fumonisin detoxification compositions and methods 失效
公开(公告)号：US06670189B2
公开(公告)日：2003-12-30
申请号：US09731393
申请日：2000-12-05
申请人： Jonathan Duvick , Joyce R. Maddox , Tracy A. Rood , Xun Wang , Benjamin A. Bowen , Jacob T. Gilliam
发明人： Jonathan Duvick , Joyce R. Maddox , Tracy A. Rood , Xun Wang , Benjamin A. Bowen , Jacob T. Gilliam
IPC分类号： C12N1582
CPC分类号： A01N63/00 , C12N9/00 , C12N9/16 , C12N9/18 , C12N15/8209 , C12N15/8242 , C12N15/8282
摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.

3. 发明授权

US06835569B2 Amino polyol oxidase amine polynucleotides and related polypeptides and methods of use 失效
标题翻译：氨基多胺胺氧化酶胺多核苷酸及相关多肽及其使用方法
公开(公告)号：US06835569B2
公开(公告)日：2004-12-28
申请号：US09770564
申请日：2001-01-26
申请人： Jonathan P. Duvick , Jacob T. Gilliam , Joyce R. Maddox , Oswald Crasta , Otto Folkerts
发明人： Jonathan P. Duvick , Jacob T. Gilliam , Joyce R. Maddox , Oswald Crasta , Otto Folkerts
IPC分类号： C12N1509
CPC分类号： C12N9/0022 , A23K10/14 , A23K10/18 , A23K30/18 , C07K2319/00 , C12N9/16 , C12N15/8209 , C12N15/8242 , C12N15/8282 , G01N2333/906
摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera. Additionally, the polynucleotide encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems.
摘要翻译：本发明提供了从Exophiala spinifera分离的酶APAO的多核苷酸和相关多肽。此外，编码APAO酶的多核苷酸可用于转化通常易受镰刀菌或其它产生毒素的真菌感染敏感的植物细胞。植物可以从转化的植物细胞中再生。另外，本发明提供在转基因植物中表达APAO和伏马菌素酯酶。以这种方式，可以生产具有降解伏马菌素的能力以及产生降解酶的能力的转基因植物。此外，本发明提供了在原核和非植物真核系统中生产APAO酶的方法。

4. 发明授权

US06514749B1 Fumonisin detoxification compositions and methods 有权
标题翻译：富马尼素解毒组合物和方法
公开(公告)号：US06514749B1
公开(公告)日：2003-02-04
申请号：US09885876
申请日：2001-06-20
申请人： Jonathan Duvick , Joyce R. Maddox , Tracy A. Rood
发明人： Jonathan Duvick , Joyce R. Maddox , Tracy A. Rood
IPC分类号： C12N114
CPC分类号： A01N63/00 , C12N9/00 , C12N9/16 , C12N9/18 , C12N15/8209 , C12N15/8242 , C12N15/8282
摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.
摘要翻译：鉴定能够降解伏马菌素的生物体的方法。可以将烟草毒素掺入到培养基中，用于选择对伏马菌素抗性的生物体和/或能够生长在作为唯一碳源的伏马毒素上。使用这种方法，已经确定了几种生物体。这些生物可用于分离酶和负责赋予伏马菌素抗性的基因。可以将该基因克隆并插入到合适的表达载体中，以使蛋白质进一步表征。此外，编码伏马菌素降解酶的DNA可用于转化通常易受镰刀菌或其他产生毒素的真菌感染敏感的植物细胞。植物可以从转化的植物细胞中再生。以这种方式，可以生产具有降解伏马菌素的能力以及产生降解酶的能力的转基因植物。还公开了谷物，谷物加工，青贮饲料，粮食作物，动物饲料和瘤胃微生物的解毒方法。

5. 发明授权

US06211435B1 Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use 有权
标题翻译：氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法
公开(公告)号：US06211435B1
公开(公告)日：2001-04-03
申请号：US09352168
申请日：1999-07-12
申请人： Jonathan P. Duvick , Jacob T. Gilliam , Joyce R. Maddox , Oswald R. Crasta , Otto Folkerts
发明人： Jonathan P. Duvick , Jacob T. Gilliam , Joyce R. Maddox , Oswald R. Crasta , Otto Folkerts
IPC分类号： C12N504
CPC分类号： C12N9/0022 , A23K10/14 , A23K10/18 , A23K30/18 , C07K2319/00 , C12N9/16 , C12N15/8209 , C12N15/8242 , C12N15/8282 , G01N2333/906
摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera. Additionally, the polynucleotide encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.
摘要翻译：本发明提供了从Exophiala spinifera分离的酶APAO的多核苷酸和相关多肽。此外，编码APAO酶的多核苷酸可用于转化通常易受镰刀菌或其它产生毒素的真菌感染敏感的植物细胞。植物可以从转化的植物细胞中再生。另外，本发明提供在转基因植物中表达APAO和伏马菌素酯酶。以这种方式，可以生产具有降解伏马菌素的能力以及产生降解酶的能力的转基因植物。此外，本发明提供了在原核和非植物真核系统中生产APAO酶的方法。还公开了谷物，谷物加工，青贮饲料，粮食作物，动物饲料和瘤胃微生物的解毒方法。

6. 发明授权

US06812380B2 Compositions and methods of zearalenone detoxification 失效
标题翻译：玉米赤霉烯酮解毒的组合和方法
公开(公告)号：US06812380B2
公开(公告)日：2004-11-02
申请号：US10107096
申请日：2002-03-26
申请人： Petr Karlovsky , Edmund H. Crane, III , Jacob T. Gilliam , Joyce R. Maddox
发明人： Petr Karlovsky , Edmund H. Crane, III , Jacob T. Gilliam , Joyce R. Maddox
IPC分类号： C12N1509
CPC分类号： C12N9/18
摘要： The present invention provides nucleotide sequences encoding zearalenone detoxification polypeptides, and methods for mycotoxin detoxification using the sequences. One method comprises stably incorporating into the genome of a plant cell, a nucleotide sequence of the present invention operably linked to a heterologous promoter and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供了编码玉米赤霉烯酮解毒多肽的核苷酸序列，以及使用该序列的霉菌毒素解毒的方法。一种方法包括稳定地并入植物细胞的基因组中，本发明的核苷酸序列可操作地连接到异源启动子并再生表达核苷酸序列的稳定转化的植物。

7. 发明授权

US06627797B1 Maize lipoxygenase polynucleotide and methods of use 失效
标题翻译：玉米脂氧合酶多核苷酸及其使用方法
公开(公告)号：US06627797B1
公开(公告)日：2003-09-30
申请号：US09810268
申请日：2001-03-16
申请人： Jon Duvick , Joyce R. Maddox , Nancy P. Keller
发明人： Jon Duvick , Joyce R. Maddox , Nancy P. Keller
IPC分类号： C12N1509
CPC分类号： C12N15/8247 , C12N15/8243 , C12N15/8282
摘要： The invention provides isolated maize lipoxygenase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering lipoxygenase concentration and/or composition of plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.
摘要翻译：本发明提供了分离的玉米脂氧合酶核酸及其编码的蛋白质。本发明提供了与改变植物脂氧合酶浓度和/或组成有关的方法和组合物。本发明还提供了重组表达盒，宿主细胞和转基因植物。

8. 发明授权

US06239330B1 Fumonisin detoxification compositions and methods 有权
公开(公告)号：US06239330B1
公开(公告)日：2001-05-29
申请号：US09262758
申请日：1999-03-04
申请人： Jonathan Duvick , Joyce R. Maddox , Xun Wang
发明人： Jonathan Duvick , Joyce R. Maddox , Xun Wang
IPC分类号： C12N1531
CPC分类号： A01N63/00 , C12N9/00 , C12N9/16 , C12N9/18 , C12N15/8209 , C12N15/8242 , C12N15/8282
摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.

9. 发明授权

US06229071B1 Fumonisin detoxification compositions and methods 失效
公开(公告)号：US06229071B1
公开(公告)日：2001-05-08
申请号：US08888950
申请日：1997-07-07
申请人： Jonathan Duvick , Joyce R. Maddox , Tracy A. Rood , Xun Wang
发明人： Jonathan Duvick , Joyce R. Maddox , Tracy A. Rood , Xun Wang
IPC分类号： A01H106
CPC分类号： A01N63/00 , C12N9/00 , C12N9/16 , C12N9/18 , C12N15/8209 , C12N15/8242 , C12N15/8282
摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.

10. 发明授权

US5792931A Fumonisin detoxification compositions and methods 失效
标题翻译：富马尼素解毒组合物和方法
公开(公告)号：US5792931A
公开(公告)日：1998-08-11
申请号：US484815
申请日：1995-06-07
申请人： Jonathan Duvick , Tracy Rood , Joyce R. Maddox , Xun Wang
发明人： Jonathan Duvick , Tracy Rood , Joyce R. Maddox , Xun Wang
IPC分类号： C12N15/09 , A01N63/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/16 , C12N9/18 , C12N15/00 , C12N15/55 , C12N15/82 , C12R1/645 , C12S3/00 , A01H5/00
CPC分类号： A01N63/00 , C12N15/8209 , C12N15/8242 , C12N15/8282 , C12N9/00 , C12N9/16 , C12N9/18
摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on flumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzyme and the gene responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for furaonisin-resistance can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain processing and in animal feed and rumen microbes are also disclosed.
摘要翻译：鉴定能够降解伏马菌素的生物体的方法。可以将鱼藤苷加入培养基中，以选择对伏马菌素具有抗性的生物体和/或能够在作为唯一碳源的烟曲霉毒素上生长。使用这种方法，已经确定了几种生物体。这些生物可用于分离酶和负责赋予伏马菌素抗性的基因。可以将该基因克隆并插入到合适的表达载体中，以使蛋白质进一步表征。此外，编码呋喃诺森抗性的DNA可用于转化通常易受镰刀菌或其它产生毒素的真菌感染的植物细胞。植物可以从转化的植物细胞中再生。以这种方式，可以生产具有降解伏马菌素的能力以及产生降解酶的能力的转基因植物。还公开了谷物加工和动物饲料和瘤胃微生物中的解毒方法。

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