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    • 7. 发明授权
    • Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use
    • 氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法
    • US06211434B1
    • 2001-04-03
    • US09352159
    • 1999-07-12
    • Jonathan P. DuvickJacob T. GilliamJoyce R. Maddox
    • Jonathan P. DuvickJacob T. GilliamJoyce R. Maddox
    • C12N504
    • C12N9/0022A23K10/14A23K10/18A23K30/18C07K2319/00C12N9/16C12N15/8209C12N15/8242C12N15/8282G01N2333/906
    • The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera and Rhinocladiella atrovirens. Additionally, the polynucleotides encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.
    • 本发明提供了从Exophiala spinifera和Rhinocladiella atvirens分离的酶APAO的多核苷酸和相关多肽。 此外,编码APAO酶的多核苷酸可用于转化通常易受镰刀菌或其它产生毒素的真菌感染敏感的植物细胞。 植物可以从转化的植物细胞中再生。 另外,本发明提供在转基因植物中表达APAO和伏马菌素酯酶。 以这种方式,可以生产具有降解伏马菌素的能力以及产生降解酶的能力的转基因植物。 此外,本发明提供了在原核和非植物真核系统中生产APAO酶的方法。 还公开了谷物,谷物加工,青贮饲料,粮食作物,动物饲料和瘤胃微生物的解毒方法。