专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5716820A Fumonisin detoxification enzymes 失效
标题翻译： Fumonisin解毒酶
公开(公告)号：US5716820A
公开(公告)日：1998-02-10
申请号：US805814
申请日：1997-02-26
申请人： Jon Duvick , Tracy Rood , Xun Wang
发明人： Jon Duvick , Tracy Rood , Xun Wang
IPC分类号： C12N15/09 , A01N63/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/16 , C12N9/18 , C12N15/00 , C12N15/55 , C12N15/82 , C12R1/645 , C12S3/00 , C12N9/14
CPC分类号： A01N63/00 , C12N15/8209 , C12N15/8242 , C12N15/8282 , C12N9/00 , C12N9/16 , C12N9/18
摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin. Using this method, several organisms have been identified. These organisms can be used to isolate the enzyme and the gene responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin-resistance can be used to transform plant cells normally susceptible to Fusarium infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin.
摘要翻译：鉴定能够降解伏马菌素的生物体的方法。可以将鱼藤子皂苷掺入到培养基中以选择对伏马菌素具有抗性的生物体。使用这种方法，已经确定了几种生物体。这些生物可用于分离酶和负责赋予伏马菌素抗性的基因。可以将该基因克隆并插入到合适的表达载体中，以使蛋白质进一步表征。此外，编码伏马毒素抗性的DNA可用于转化通常易受镰刀菌感染的植物细胞。植物可以从转化的植物细胞中再生。以这种方式，可以生产具有降解伏马菌素能力的转基因植物。

2. 发明授权

US5792931A Fumonisin detoxification compositions and methods 失效
标题翻译：富马尼素解毒组合物和方法
公开(公告)号：US5792931A
公开(公告)日：1998-08-11
申请号：US484815
申请日：1995-06-07
申请人： Jonathan Duvick , Tracy Rood , Joyce R. Maddox , Xun Wang
发明人： Jonathan Duvick , Tracy Rood , Joyce R. Maddox , Xun Wang
IPC分类号： C12N15/09 , A01N63/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/16 , C12N9/18 , C12N15/00 , C12N15/55 , C12N15/82 , C12R1/645 , C12S3/00 , A01H5/00
CPC分类号： A01N63/00 , C12N15/8209 , C12N15/8242 , C12N15/8282 , C12N9/00 , C12N9/16 , C12N9/18
摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on flumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzyme and the gene responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for furaonisin-resistance can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain processing and in animal feed and rumen microbes are also disclosed.
摘要翻译：鉴定能够降解伏马菌素的生物体的方法。可以将鱼藤苷加入培养基中，以选择对伏马菌素具有抗性的生物体和/或能够在作为唯一碳源的烟曲霉毒素上生长。使用这种方法，已经确定了几种生物体。这些生物可用于分离酶和负责赋予伏马菌素抗性的基因。可以将该基因克隆并插入到合适的表达载体中，以使蛋白质进一步表征。此外，编码呋喃诺森抗性的DNA可用于转化通常易受镰刀菌或其它产生毒素的真菌感染的植物细胞。植物可以从转化的植物细胞中再生。以这种方式，可以生产具有降解伏马菌素的能力以及产生降解酶的能力的转基因植物。还公开了谷物加工和动物饲料和瘤胃微生物中的解毒方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式