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1. 发明授权

US11684921B1 Pocket detection pouch 有权
公开(公告)号：US11684921B1
公开(公告)日：2023-06-27
申请号：US16988368
申请日：2020-08-07
申请人： Leidos, Inc. , Jennifer W. Sekowski
发明人： Kelley D. Betts , Jennifer W. Sekowski
IPC分类号： B01L3/00 , G01N33/53 , G01N1/02
CPC分类号： B01L3/502715 , G01N1/02 , G01N33/5302 , B01L2200/027 , B01L2200/0689 , B01L2200/10 , B01L2300/04 , B01L2300/0636 , B01L2300/0809 , B01L2300/123 , B01L2300/126 , G01N2001/028
摘要： The pocket detection platform (PDP) detects pathogens, toxins and chemicals of interest simultaneously by way of a multi-channeled, soft see-through plastic pouch design that consists of inner and outer compartments that promote compartmentalization and/or unidirectional sample flow. The platform enables the concurrent running of multiple detection assay techniques such as lateral flow immunoassays (LFI), Isothermal molecular assays (i.e., Recombinase Polymerase Amplification, or RPA) and/or paper-based chemical assays (i.e., M8, pH paper) from a single wet or dry sample with minimal sample processing. The PDP reduces soldier overburden by decreasing size weight, and power (SWaP) as well as training time, electronic burden, while providing a flexible, customizable assay platform that can be rapidly produced, assembled, sustained, and when contaminated, easily to dispose of.

2. 发明授权

US07629129B1 Hair follicle bulb as a biodosimeter 失效
标题翻译：毛囊泡作为生物量表
公开(公告)号：US07629129B1
公开(公告)日：2009-12-08
申请号：US11416509
申请日：2006-04-26
申请人： Jennifer W. Sekowski , Amanda E. Chambers
发明人： Jennifer W. Sekowski , Amanda E. Chambers
IPC分类号： G01N33/53
CPC分类号： G01N33/5082 , G01N33/5014
摘要： The various embodiments provide method of using hair follicle bulbs as biodosimeters for the detection of chemical exposure. The methods described herein utilize intact, plucked hair follicle bulbs and can be used to monitor real-time or near real-time changes in the levels of specific follicular bulb biomarkers to determine exposure to toxicants. By utilizing the living, responsive cells in the plucked hair follicle bulb in an immunohistochemical (IHC) analysis, the various embodiments mitigate the risks of false positives associated with segmental hair analysis and avoid the more invasive collection required for serum and urinalysis.
摘要翻译：各种实施方案提供了使用毛囊泡作为生物测定仪来检测化学暴露的方法。本文描述的方法利用完整的拔毛毛囊，并且可用于监测特异性滤泡生物标志物的水平的实时或接近实时变化以确定暴露于有毒物质。通过在免疫组织化学（IHC）分析中利用采摘的毛囊中的活的响应细胞，各种实施方案减轻了与分段毛发分析相关的假阳性的风险，并避免了血清和尿分析所需的更多的侵入性收集。

3. 发明授权

US07625708B1 Immunohistochemistry method for intact plucked hair follicles 失效
标题翻译：完整的拔毛毛囊的免疫组织化学方法
公开(公告)号：US07625708B1
公开(公告)日：2009-12-01
申请号：US11449180
申请日：2006-06-07
申请人： Jennifer W. Sekowski , Amanda E. Chambers
发明人： Jennifer W. Sekowski , Amanda E. Chambers
IPC分类号： G01N33/53
CPC分类号： G01N33/5014 , G01N33/5044
摘要： The various embodiments provide method of using hair follicle bulbs as biodosimeters for the detection of chemical exposure. The methods described herein utilize intact, plucked hair follicle bulbs and can be used to monitor real-time or near real-time changes in the levels of specific follicular bulb biomarkers to determine exposure to toxicants. By utilizing the living, responsive cells in the plucked hair follicle bulb in an immunohistochemical (IHC) analysis, the various embodiments mitigate the risks of false positives associated with segmental hair analysis and avoid the more invasive collection required for serum and urinalysis.
摘要翻译：各种实施方案提供了使用毛囊泡作为生物测定仪来检测化学暴露的方法。本文描述的方法利用完整的拔毛毛囊，并且可用于监测特异性滤泡生物标志物的水平的实时或接近实时变化以确定暴露于有毒物质。通过在免疫组织化学（IHC）分析中利用采摘的毛囊中的活的响应细胞，各种实施方案减轻了与分段毛发分析相关的假阳性的风险，并避免了血清和尿分析所需的更多的侵入性收集。

4. 发明授权

US6063575A Assay for measuring the activity and fidelity of DNA replication and kit therefor 失效
标题翻译：测定DNA复制的活性和保真度的试剂盒
公开(公告)号：US6063575A
公开(公告)日：2000-05-16
申请号：US45624
申请日：1998-03-20
申请人： Jennifer W. Sekowski , Linda H. Malkas , Robert J. Hickey
发明人： Jennifer W. Sekowski , Linda H. Malkas , Robert J. Hickey
IPC分类号： A61K38/00 , C07K16/40 , G01N33/50 , G01N33/574 , C12Q1/68 , C12P19/34
CPC分类号： G01N33/5091 , C07K16/40 , G01N33/5008 , G01N33/5011 , G01N33/5014 , G01N33/5088 , G01N33/57488 , A61K38/00 , G01N2800/52
摘要： Mercuric ion is cytotoxic and mutagenic to cells. However, the mechanisms of mercuric ion-induced cytotoxicity are not well understood. Studies have suggested that these effects may be due in part to the alteration and inhibition of a variety of cellular processes including DNA replication, DNA repair, RNA transcription, and protein synthesis. However, prior art studies utilizing whole cells, cell extracts, or purified DNA polymerases to examine these activities are not able to specifically identify the precise mechanism or site of the effect or adequately represent the highly ordered environment in which DNA replication occurs in the intact cell. We disclose a novel method for measuring the activity and fidelity of DNA replication using the complex of proteins called the DNA synthesome, an isolated multiprotein form of DNA polymerase. The DNA synthesome is a highly organized complex of proteins capable of supporting all phases of SV 40 origin-specific DNA replication in vitro. The assay and kit described herein can be used both to examine the mechanism of cytotoxicity and mutagenicity and to identify compounds that alter the activity and fidelity of the DNA synthetic apparatus such as heavy metal ion, particularly mercuric ion. The assay and kit can be used to rapidly screen compounds for toxic activity.
摘要翻译：汞离子对细胞具有细胞毒性和致突变性。然而，汞离子诱导的细胞毒性的机制尚不清楚。研究表明，这些作用可能部分归因于多种细胞过程的改变和抑制，包括DNA复制，DNA修复，RNA转录和蛋白质合成。然而，利用全细胞，细胞提取物或纯化的DNA聚合酶来检查这些活性的现有技术研究不能特异性地鉴定效应的精确机制或位点或充分代表在完整细胞中发生DNA复制的高度有序的环境。我们公开了一种使用称为DNA合成物的蛋白质复合物（一种分离的多聚蛋白形式的DNA聚合酶）测量DNA复制的活性和保真度的新方法。 DNA合成物是一种高度组织的蛋白质复合物，能够在体外支持SV40起源特异性DNA复制的所有相。本文所述的测定和试剂盒可用于检查细胞毒性和致突变性的机制，并鉴定改变DNA合成装置如重金属离子，特别是汞离子的活性和保真度的化合物。测定和试剂盒可用于快速筛选化合物的毒性活性。

5. 发明授权

US09115240B2 Color changing polymer films for detecting chemical and biological targets 有权
标题翻译：用于检测化学和生物靶标的变色聚合物膜
公开(公告)号：US09115240B2
公开(公告)日：2015-08-25
申请号：US13484533
申请日：2012-05-31
申请人： Peter Kofinas , Omar B. Ayyub , Jennifer W. Sekowski , Ta-I Yang
发明人： Peter Kofinas , Omar B. Ayyub , Jennifer W. Sekowski , Ta-I Yang
IPC分类号： C08F226/06 , G01N21/25 , C08F293/00 , G01N21/78 , G01N21/77
CPC分类号： G01N21/78 , C08F8/44 , C08F293/00 , G01N2021/7723 , Y10T436/143333 , Y10T436/144444 , Y10T436/173076 , Y10T436/206664 , C08F226/06 , C08F212/08 , C08F8/42 , C08F8/30
摘要： A sensor system, and a method of detecting a target analyte, comprises a chemically functionalized block copolymer, and a target analyte. The block copolymer exhibits a color change in the visible spectrum upon exposure to the target analyte.
摘要翻译：传感器系统和检测目标分析物的方法包括化学官能化的嵌段共聚物和目标分析物。嵌段共聚物在暴露于目标分析物时显示可见光谱的颜色变化。

6. 发明申请

US20120309047A1 Color Changing Polymer Films for Detecting Chemical and Biological Targets 有权
标题翻译：用于检测化学和生物目标的变色聚合物膜
公开(公告)号：US20120309047A1
公开(公告)日：2012-12-06
申请号：US13484533
申请日：2012-05-31
申请人： Peter Kofinas , Omar B. Ayyub , Jennifer W. Sekowski , Ta-I Yang
发明人： Peter Kofinas , Omar B. Ayyub , Jennifer W. Sekowski , Ta-I Yang
IPC分类号： C08F226/06 , G01N21/25
CPC分类号： G01N21/78 , C08F8/44 , C08F293/00 , G01N2021/7723 , Y10T436/143333 , Y10T436/144444 , Y10T436/173076 , Y10T436/206664 , C08F226/06 , C08F212/08 , C08F8/42 , C08F8/30
摘要： A sensor system, and a method of detecting a target analyte, comprises a chemically functionalized block copolymer, and a target analyte. The block copolymer exhibits a color change in the visible spectrum upon exposure to the target analyte.
摘要翻译：传感器系统和检测目标分析物的方法包括化学官能化的嵌段共聚物和目标分析物。嵌段共聚物在暴露于目标分析物时显示可见光谱的颜色变化。

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