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1. 发明申请

US20120249770A1 METHOD FOR AUTOMATICALLY FOCUSING A MICROSCOPE ON A PREDETERMINED OBJECT AND MICROSCOPE FOR AUTOMATIC FOCUSING 审中-公开
标题翻译：用于自动聚焦的预定对象和显微镜上的显微镜的自动聚焦方法
公开(公告)号：US20120249770A1
公开(公告)日：2012-10-04
申请号：US13395441
申请日：2010-08-20
申请人： Peter Hamm , Karl-Hans Englmeier , Thomas Waschulzik , Horst Wolff
发明人： Peter Hamm , Karl-Hans Englmeier , Thomas Waschulzik , Horst Wolff
IPC分类号： H04N7/18
CPC分类号： G02B21/244
摘要： A method for automatic focusing of a microscope on a predetermined object in a specimen to be examined may include a) producing a set of criteria to be satisfied for the predetermined object using at least one training image of the object, b) producing a first image of the specimen to be examined which contains the predetermined object with the microscope in a first focal position, c) ascertaining the section or sections of the first image, which in each case satisfies or satisfy the set of criteria according to step a), and defining each ascertained section as object area of the first image, d) producing further images of the specimen with the microscope in different focal positions, e) determining the optimum focal position(s) using the further images, wherein, for this purpose, in all images only the partial region or partial regions which correspond to the object area or object areas is/are evaluated, f) focusing the microscope on at least one of the optimum focal position(s) determined in step e).
摘要翻译：一种显微镜自动聚焦在待检查样本中的预定物体上的方法可以包括：a）使用该物体的至少一个训练图像来产生一组对于预定物体满足的标准，b）产生第一图像在第一焦点位置用显微镜包含预定物体的检查样本，c）确定在每一种情况下符合或满足根据步骤a）的一组标准的第一图像的部分或部分，以及将每个确定的部分定义为第一图像的对象区域，d）用不同焦点位置的显微镜产生样本的另外的图像，e）使用另外的图像确定最佳焦点位置，其中，为此目的，所有图像仅评估对应于对象区域或对象区域的部分区域或部分区域，f）将显微镜聚焦在最佳焦点位置抑制器中的至少一个上在步骤e）中开采。

2. 发明授权

US4583921A Plunger pump 失效
标题翻译：柱塞泵
公开(公告)号：US4583921A
公开(公告)日：1986-04-22
申请号：US687615
申请日：1984-12-28
申请人： Horst Wolff , Franz Hani
发明人： Horst Wolff , Franz Hani
IPC分类号： F04B1/12 , F04B1/14 , F04B53/08 , F04B1/18 , F01B31/10 , F04B39/02
CPC分类号： F04B1/14 , F04B1/124 , F04B1/141 , F04B1/146 , F04B53/08
摘要： A pump which operates with several plungers is described with the plunger preferably being actuated via an eccentric drive unit and, having regard to the particular volume of flow per unit time, using several small plungers rather than a lesser number of comparatively large plungers, and accordingly operating with a large number of small volume displacements at a comparatively high speed of rotation. In this arrangement the suction chamber is centrally arranged in the pump body and the high pressure outlets of the pump chambers, which open into a common pressure chamber of ring-like construction, extend at least substantially in a radial direction.
摘要翻译：描述了用多个柱塞操作的泵，柱塞优选地通过偏心驱动单元致动，并且考虑到每单位时间的特定流量，使用几个小柱塞，而不是较少数量的较大的柱塞，因此以较高的旋转速度运行大量的小体积位移。在这种布置中，抽吸室居中布置在泵体中，并且泵室的高压出口（其敞开到具有环形结构的公共压力室）至少在径向方向上延伸。

3. 发明授权

US09690087B2 Method and apparatus for defining a z-range in a sample, in which a z-stack of the sample is to be recorded by means of a microscope 有权
公开(公告)号：US09690087B2
公开(公告)日：2017-06-27
申请号：US14117350
申请日：2012-04-04
申请人： Horst Wolff , Daniel Svejdar , Markus Eichinger
发明人： Horst Wolff , Daniel Svejdar , Markus Eichinger
IPC分类号： G02B21/36 , G02B21/00 , G02B21/24
CPC分类号： G02B21/365 , G02B21/006 , G02B21/244
摘要： A method for defining a z-range in a sample in which a z-stack of the sample is to be recorded by means of a microscope is provided, wherein the z-range is defined automatically on the basis of a z-value situated in the sample and taking at least one predetermined parameter into account.

4. 发明申请

US20080161199A1 Fusion Proteins and Methods for Determining Protein-Protein-Interactions in Living Cells and Cell Lysates, Nucleic Acids Encoding these Fusion Proteins, as well as Vectors and Kits Containing These 审中-公开
标题翻译：用于确定活细胞和细胞裂解物中的蛋白质相互作用的融合蛋白质和方法，编码这些融合蛋白的核酸，以及含有这些融合蛋白的载体和试剂盒
公开(公告)号：US20080161199A1
公开(公告)日：2008-07-03
申请号：US11793161
申请日：2005-12-22
申请人： Horst Wolff , Andrea Brebeck , Manja Ziegler , Ruth Brack-Werner
发明人： Horst Wolff , Andrea Brebeck , Manja Ziegler , Ruth Brack-Werner
IPC分类号： C40B30/10 , C12Q1/02
CPC分类号： C07K14/43595 , C07K2319/00 , C12N15/62 , G01N33/542 , G01N33/582
摘要： The present invention refers to inventive fusion proteins, to a method for detecting protein-protein-interactions in living cells and cell lysates using these inventive fusion proteins. The present invention also refers to a screening method for identifying compounds suitable to modify, i.e. inhibit or enhance, protein-protein-interaction using these fusion proteins. Additionally a method for detecting cells is disclosed comprising an unknown protein that interacts with a known protein. Along with the inventive fusion proteins used for these methods encoding nucleic acids, corresponding vectors and host cells transfected accordingly are disclosed herewith.
摘要翻译：本发明涉及本发明的融合蛋白，涉及使用这些本发明的融合蛋白检测活细胞和细胞裂解物中的蛋白质 - 蛋白质相互作用的方法。本发明还涉及用于鉴定适合于使用这些融合蛋白修饰，即抑制或增强蛋白质 - 蛋白质相互作用的化合物的筛选方法。另外，公开了一种用于检测细胞的方法，其包括与已知蛋白质相互作用的未知蛋白质。随着本文公开了用于编码核酸的这些方法的本发明融合蛋白，相应的载体和相应转染的宿主细胞。

5. 发明授权

US07183047B2 Reporter gene construct for the detection of HIV Rev and HIV Tat 失效
标题翻译：报告基因构建用于检测艾滋病毒感染和艾滋病毒
公开(公告)号：US07183047B2
公开(公告)日：2007-02-27
申请号：US10176010
申请日：2002-06-20
申请人： Ruth Brack-Werner , Markus Neumann , Horst Wolff , Volker Erfle
发明人： Ruth Brack-Werner , Markus Neumann , Horst Wolff , Volker Erfle
IPC分类号： C12Q1/00 , C12Q1/02 , C12Q1/68 , C12N15/33
CPC分类号： C07K14/005 , C07K2319/00 , C12N15/85 , C12N2740/16322 , C12N2830/50
摘要： The present invention relates to a reporter gene construct for the detection of the HIV Rev and HIV Tat proteins. Furthermore, the invention relates to a functionality test method for Rev and Rev fusion proteins prepared in a recombinant manner, a method of screening for sequences of different origin for their activity as an instability element, a method of screening for sequences which cause the transport out of the nucleus into the cytoplasm by binding to cellular or other viral shuttle proteins, as well as to a method for the detection of HIV-infected cells. The reporter gene construct according to the present invention, after it has been introduced into cells, in the presence of HIV Rev and HIV Tat proteins results in the formation of reporter proteins which may be used for quantitative/qualitative detection of the HIV Rev and HIV Tat proteins.
摘要翻译：本发明涉及用于检测HIV Rev和HIV Tat蛋白的报告基因构建体。此外，本发明涉及以重组方式制备的Rev和Rev融合蛋白的功能性测试方法，筛选不稳定序列的不同来源的序列作为不稳定性元件的活性的方法，筛选引起转运的序列的方法通过与细胞或其他病毒穿梭蛋白结合而进入细胞质，以及用于检测HIV感染细胞的方法。根据本发明的报道基因构建体在其被引入细胞之后，在HIV Rev和HIV Tat蛋白的存在下导致报告蛋白的形成，其可用于HIV Rev和HIV的定量/定性检测 Tat蛋白。

6. 发明授权

US4456439A High pressure plunger pump 失效
标题翻译：高压柱塞泵
公开(公告)号：US4456439A
公开(公告)日：1984-06-26
申请号：US318266
申请日：1981-11-04
申请人： Horst Wolff
发明人： Horst Wolff
IPC分类号： F04B53/10 , F04B9/04 , F04B23/06 , F04B53/16 , F04B39/14 , F04B21/00
CPC分类号： F04B53/164
摘要： A high pressure plunger pump, in particular for forwarding water and water with additives, comprises a plurality of plungers 20 which are driven by a crankshaft journalled in a drive housing 10. The free ends of the plungers 20 are each guided in respective pump chambers 22 of an induction-discharge housing 12 which is connected to the drive housing 10. The induction-discharge housing 12 is a one piece housing of generally L-shaped cross-section. The pump chambers 22 are arranged in one limb of the L-shaped housing and communicate with an induction chamber 27 via spring biased inlet valves 27 provided in axial extensions of the pump chambers. A discharge chamber 31 extends through the other limb of the L-shaped housing and is fed from the pump chambers via outlet valves 29. Both the inlet and the outlet valves 25, 29 are secured in position by respective threaded plugs. The use of a one piece L-shaped housing brings a number of substantial benefits. The housing is particularly convenient to manufacture, is very stiff, allows a significant weight saving, can be conveniently attached to the drive housing and allows ready access to all components for servicing and repair purposes. In a preferred arrangement high and low pressure seal 23, 34 are associated with each of the plungers 20 and the low pressure seals are arranged in a scavenge circulation established by connection passages 32, 32' extending between the induction chamber 27 and chambers accommodating the low pressure seals 34.
摘要翻译：高压柱塞泵，特别是用于将水和水转移到添加剂中，包括多个柱塞20，其由安装在驱动壳体10中的曲轴驱动。柱塞20的自由端分别在相应的泵室22中被引导感应放电壳体12连接到驱动壳体10.感应放电壳体12是大体为L形横截面的一体式壳体。泵室22布置在L形壳体的一个支腿中，并通过设置在泵室的轴向延伸部中的弹簧偏置的入口阀27与感应室27连通。排出室31延伸穿过L形壳体的另一个分支，并且经由出口阀29从泵室进料。入口阀25和出口阀29通过相应的螺纹塞固定就位。使用单件L形外壳带来了许多实质的好处。外壳特别方便制造，非常坚硬，可以显着减轻重量，可以方便地连接到驱动器外壳上，并允许随时访问所有部件进行维修和修理。在优选的布置中，高压和低压密封件23,34与每个柱塞20相关联，并且低压密封件布置在通过连接通道32,32'建立的清扫循环中，所述连接通道32,32'在诱导室27和容纳低压压力密封件34。

7. 发明授权

US4301275A Process and device for continuously treating with gases aqueous dispersions of polyvinyl chloride 失效
标题翻译：用气体连续处理聚氯乙烯水分散体的方法和装置
公开(公告)号：US4301275A
公开(公告)日：1981-11-17
申请号：US151404
申请日：1980-05-19
申请人： Christoph Heinze , Franz Botsch , Horst Wolff
发明人： Christoph Heinze , Franz Botsch , Horst Wolff
IPC分类号： C08F14/00 , B01J8/10 , B01J19/18 , C08F6/00 , C08F6/06 , C08F6/10 , C08F6/24 , C08F6/16
CPC分类号： B01J8/10 , C08F6/003 , B01J2219/182
摘要： In aqueous dispersions on the basis of polyvinyl chloride the content of residual monomers is reduced by the countercurrent method by contacting the rapidly moved surface of the dispersion with secluded intensely mixed gas atmospheres in several successive chambers. To carry out the process a device is used which permits to obtain under mild conditions residual monomer contents of 10 ppm and therebelow.
摘要翻译：在基于聚氯乙烯的水性分散体中，通过逆流法通过使分散体的快速移动的表面与几个连续的室中的僻静的强烈混合的气体气氛接触来降低残余单体的含量。为了进行该方法，使用允许在温和条件下获得10ppm及以下残留单体含量的装置。

8. 发明授权

US4158092A Process for the manufacture of vinyl chloride polymer dispersions with a low monomer content 失效
标题翻译：用于制造具有低单体含量的氯乙烯聚合物分散体的方法
公开(公告)号：US4158092A
公开(公告)日：1979-06-12
申请号：US845479
申请日：1977-10-26
申请人： Franz Botsch , Christoph Heinze , Horst Wolff
发明人： Franz Botsch , Christoph Heinze , Horst Wolff
IPC分类号： B01D1/16 , B01D3/06 , B01D3/08 , B01D3/34 , B01D3/38 , B01J10/02 , B01J19/18 , C08F6/00 , C08F6/16
CPC分类号： C08F6/16 , B01D1/16 , B01D3/06 , B01D3/065 , B01D3/08 , B01D3/346 , B01D3/38 , B01J10/02 , B01J19/1862 , B01J19/1881 , C08F6/003 , B01J2219/00094 , B01J2219/00103 , Y10S159/10 , Y10T428/31855
摘要： Polymer dispersions are subject to a spray treatment to reduce the monomer content thereof. The dispersions are sprayed against the wall of the vessel by means of at least one spray nozzle.The residual monomer content of polymer dispersions is further reduced by spraying them in at least two series connected vessels, conducting the escaping gaseous constituents in one direction and the dispersion in opposite direction from one vessel to the next. The process is little liable to troubles and ensures a very effective monomer removal without thermal damage of the polymer.
摘要翻译：聚合物分散体经受喷雾处理以降低其单体含量。通过至少一个喷嘴将分散体喷射到容器的壁上。

9. 发明授权

US4561828A Pump for forwarding liquids 失效
标题翻译：用于转运液体的泵
公开(公告)号：US4561828A
公开(公告)日：1985-12-31
申请号：US495855
申请日：1983-05-18
申请人： Horst Wolff , Franz Hani
发明人： Horst Wolff , Franz Hani
IPC分类号： F04B49/10 , F04B53/10 , F04B53/14 , F04B49/08 , F04B7/04 , F04B39/10 , F16K25/00
CPC分类号： F04B53/1032 , F04B49/10
摘要： A pump for forwarding liquids is described which has at least one pump chamber with inlet and outlet valves and also a plunger which periodically varies the volume of the pump chamber. To avoid the problems of dry running with very rapidly running pumps, and in order to overcome the resulting danger of destruction, the pump is arranged so that the closing force of at least one inlet valve of each pump chamber is controlled in dependence on the movement of the displacement member.
摘要翻译：描述了用于传送液体的泵，其具有至少一个具有入口和出口阀的泵室以及周期性地改变泵室体积的柱塞。为了避免使用非常快速运行的泵的干运行的问题，并且为了克服所造成的破坏危险，泵被布置成使得每个泵室的至少一个入口阀的关闭力根据运动来控制的位移构件。

10. 发明申请

US20140232844A1 METHOD AND APPARATUS FOR DEFINING A Z-RANGE IN A SAMPLE, IN WHICH A Z-STACK OF THE SAMPLE IS TO BE RECORDED BY MEANS OF A MICROSCOPE 有权
标题翻译：用于在样品中定义Z-范围的方法和装置，其中样品的Z型堆叠将通过MICROSCOPE记录
公开(公告)号：US20140232844A1
公开(公告)日：2014-08-21
申请号：US14117350
申请日：2012-04-04
申请人： Horst Wolff , Daniel Svejdar , Markus Eichinger
发明人： Horst Wolff , Daniel Svejdar , Markus Eichinger
IPC分类号： G02B21/36
CPC分类号： G02B21/365 , G02B21/006 , G02B21/244
摘要： A method for defining a z-range in a sample in which a z-stack of the sample is to be recorded by means of a microscope is provided, wherein the z-range is defined automatically on the basis of a z-value situated in the sample and taking at least one predetermined parameter into account.
摘要翻译：提供了一种用于通过显微镜来定义待样品的z-堆叠的样品中z范围的方法，其中z范围是根据位于该样本并考虑至少一个预定参数。

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