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    • 3. 发明申请
    • CENTRIFUGAL DEVICE AND METHOD FOR OVA DETECTION
    • 用于OVA检测的离心装置和方法
    • US20090258411A1
    • 2009-10-15
    • US12400364
    • 2009-03-09
    • Henry PetithoryJames KellandKevin SullivanLinda Martin
    • Henry PetithoryJames KellandKevin SullivanLinda Martin
    • C12N1/02C12M1/00
    • B04B5/04A61B10/0038B01L3/0272B01L3/5021B01L3/5029B01L2300/0681B04B5/0414B04B7/02B04B11/04B04B11/06B04B2005/0464B04B2005/0478
    • A centrifugal device and method are provided for the separation of buoyant material such as parasitic ova from fecal matter. A rotor assembly, rotatable about its central axis in a centrifuge, includes a housing with a centrally located top opening leading to a centrally located mixing chamber. An annular sediment chamber is provided, also coaxial about the central axis, connected by a passage with the mixing chamber. A coring assembly is used to retrieve and insert a fecal sample into the mixing chamber for mixing with a flotation fluid. During centrifugation, heavier fecal components pass radially outwardly to the sediment chamber while the ova collect on the inward surface of the flotation fluid. After centrifugation, more flotation fluid is added, if needed, until a meniscus forms at the top opening. A coverslip is placed over the top opening and the ova float to the surface of the fluid and adhere to the coverslip. The coverslip is removed and the ova detected using standard microscopy procedures. In another aspect, a centrifugal device is provided in which the ova are delivered through centrifugation to a pipette tip for dispensing onto a microscope slide or coverslip.
    • 提供了一种离心装置和方法,用于将浮力物质(例如寄生虫卵)与粪便分离。 在离心机中围绕其中心轴线旋转的转子组件包括具有中心定位的顶部开口的壳体,其通向位于中心的混合室。 提供环形沉积室,其也围绕中心轴线共轴,通过与混合室的通道连接。 取芯组件用于将粪便样品回收并插入到混合室中以与浮选流体混合。 在离心过程中,较重的粪便部件径向向外通向沉淀室,而卵子则聚集在浮选流体的内表面上。 离心后,如果需要,加入更多的浮选液,直到在顶部开口处形成弯液面。 将盖玻片放置在顶部开口上方,并且ova浮动到流体表面并粘附到盖玻片上。 取出盖玻片,使用标准显微镜检查检测到ova。 在另一方面,提供一种离心装置,其中通过离心将卵子输送到移液管尖端以分配到显微镜载玻片或盖玻片上。