专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20050239087A1 Multiply-primed amplification of nucleic acid sequences 审中-公开
标题翻译：核酸序列的多重引物扩增
公开(公告)号：US20050239087A1
公开(公告)日：2005-10-27
申请号：US10835140
申请日：2004-04-29
申请人： Haiguang Xiao , Scott Hamilton
发明人： Haiguang Xiao , Scott Hamilton
IPC分类号： C12N20060101 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6844 , C12Q2537/143 , C12Q2525/117 , C12Q2525/107 , C12Q2531/125 , C12Q2525/179
摘要： Improved processes for the amplification of target DNA sequences in the form of single or double stranded DNA molecules, especially those present in colony and plaque extracts, using multiple specific and/or random sequence oligonucleotide primers are disclosed along with methods for detecting such amplified target sequences wherein some or all of the deoxyribonucleotides are replaced by deoxyribonucleotide analogues that reduce the Tm of the amplified product. The product of this amplification is used for DNA sequencing and other analyses that involve hybridization. Kits containing components for use in the invention is also described. Also described are further uses of this amplified DNA in sequencing, single base substitution detection, modifying the restriction enzyme fragmentation patterns and other molecular biology applications.
摘要翻译：公开了使用多个特异性和/或随机序列寡核苷酸引物扩增单链或双链DNA分子，特别是存在于菌落和斑块提取物中的靶DNA序列的改进方法以及用于检测这些扩增的靶序列其中一些或所有脱氧核糖核苷酸被降低扩增产物的Tm的脱氧核糖核苷酸类似物代替。该扩增产物用于DNA测序和涉及杂交的其他分析。还描述了包含用于本发明的组分的试剂盒。还描述了该扩增的DNA在测序，单碱基取代检测，修饰限制酶断裂模式和其他分子生物学应用中的进一步使用。

2. 发明申请

US20100105109A1 MULTIPLY-PRIMED AMPLIFICATION OF CIRCULAR NUCLEIC ACID SEQUENCES 审中-公开
标题翻译：圆形核酸序列的多重增殖放大
公开(公告)号：US20100105109A1
公开(公告)日：2010-04-29
申请号：US12532228
申请日：2008-03-18
申请人： Haiguang Xiao , John R. Nelson , Galina Chernaya , Haiying Wang , Paul Mitsis , Gyanendra Kumar , Carl F. Fuller , Yuyang Christine Cai
发明人： Haiguang Xiao , John R. Nelson , Galina Chernaya , Haiying Wang , Paul Mitsis , Gyanendra Kumar , Carl F. Fuller , Yuyang Christine Cai
IPC分类号： C12P19/34
CPC分类号： C12Q1/6806 , C12Q1/6844 , C12Q2531/125 , C12Q2521/319
摘要： Improved processes for the amplification of target DNA sequences in the form of single or double stranded circular DNA molecules, especially those present in colony and plaque extracts, using multiple specific and/or random sequence oligonucleotide primers are disclosed. The product of this amplification is used for analysis by restriction enzyme digestion or DNA sequencing and other analyses that involve hybridization. Kits containing components for use in the method are also described. Also described are further uses of this amplified DNA in sequencing, genotyping and haplotyping, and other molecular biology applications.
摘要翻译：公开了使用多个特异性和/或随机序列寡核苷酸引物扩增单链或双链环状DNA分子，特别是存在于菌落和斑块提取物中的靶DNA序列的改进方法。该扩增产物用于通过限制酶消化或DNA测序和涉及杂交的其他分析进行分析。还描述了包含用于该方法的组分的试剂盒。还描述了这种扩增的DNA在测序，基因分型和单元分型以及其他分子生物学应用中的进一步使用。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式