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    • 8. 发明申请
    • Quantitative, multispectral image analysis of tissue specimens stained with quantum dots
    • 用量子点染色的组织标本的定量,多光谱图像分析
    • US20110235880A1
    • 2011-09-29
    • US13134303
    • 2011-06-03
    • G. Scott LettNed C. HaubeinGary Pestano
    • G. Scott LettNed C. HaubeinGary Pestano
    • G06K9/00
    • G01N21/6428G01N21/6458G01N2021/6421G01N2021/6423G01N2021/6439G06F3/04842G06F3/04847
    • A biological sample such as a tissue section is stained with one or more quantum dots and possibly other fluorophores (total number of fluorophores N). A camera coupled to a microscope generates an image of the specimen at a plurality of different wavelengths within the emission spectral band of the N fluorophores. An analysis module calculates coefficients C1 . . . CN at each pixel from the set of images and reference spectral data for the N fluorophores. The coefficients C1 . . . CN are related to the concentration of each of the individual fluorophores at each pixel location. Morphological processing instructions find biological structures, e.g., cells, cellular components, genes, etc., in the images of the specimen. Quantitative analysis is performed on the identified biological structures. A display module displays the quantitative analysis results to the user, along with images of the specimen. The images can include images constructed from one or more of the coefficients C1 . . . CN. The quantitative analysis display includes histograms of the biological structures, scatter plots of fluorophore concentrations, statistical data, spectral data and still others.
    • 生物样品如组织切片用一个或多个量子点和可能的其它荧光团(总共荧光团数N)染色。 耦合到显微镜的照相机在N个荧光团的发射光谱带内以多个不同的波长产生样本的图像。 分析模块计算系数C1。 。 。 CN在来自该组图像的每个像素和N个荧光团的参考光谱数据。 系数C1。 。 。 CN与每个像素位置处的各个荧光团的浓度有关。 形态学处理指令在样本的图像中发现生物结构,例如细胞,细胞组分,基因等。 对所鉴定的生物结构进行定量分析。 显示模块与用户的图像一起显示定量分析结果。 图像可以包括由一个或多个系数C1构成的图像。 。 。 CN。 定量分析显示包括生物结构的直方图,荧光团浓度的散点图,统计数据,光谱数据等。