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1. 发明授权

US08435558B1 Ultrasound enhancement of drug release across non-ionic surfactant membranes 有权
标题翻译：通过非离子表面活性剂膜超声增强药物释放
公开(公告)号：US08435558B1
公开(公告)日：2013-05-07
申请号：US13185100
申请日：2011-07-18
申请人： Elizabeth Hood , Joel A. Strom , Michael VanAuker
发明人： Elizabeth Hood , Joel A. Strom , Michael VanAuker
IPC分类号： A61K9/127 , A61K9/48
CPC分类号： A61K9/1272 , A61K9/0009 , A61K41/0028 , A61K49/0002 , A61K49/22
摘要： A method of targeted drug delivery and imaging using nonionic surfactant vesicles (niosomes) in combination with ultrasound is presented. Niosomes have potential applications in targeted drug delivery and imaging because of their ability to encapsulate therapeutic agents and their enhanced uptake by physiological membranes. The niosomes may be administered to the subject via catheter. Ultrasound may be used to mediate delivery non-invasively by altering the niosome membrane structure. Niosomes composed of polyoxyethylene sorbitan monostearate (Tween 61), cholesterol, and dicetyl phosphate were synthesized via a thin film hydration technique and used for encapsulation studies. Carboxyfluorescein dye (CF) was used as a drug model to demonstrate delivery. The amount of dye in the niosomes, the concentration of the vesicles, and their mean particle size after each 5 minute incremental exposure to ultrasound was monitored. It was found that ultrasound at specific frequencies can reversibly permeabilize the lipid membrane of niosomes to allow the controlled release of a compound without destroying the niosome structure.
摘要翻译：提出了一种使用非离子表面活性剂囊泡（niosomes）与超声结合的靶向药物递送和成像方法。 Niosomes由于其能够包封治疗剂和增强生理膜吸收的能力，在靶向药物递送和成像中具有潜在应用。可以通过导管向受试者施用niosomes。超声波可用于通过改变niosome膜结构非侵入性地介导递送。通过薄膜水合技术合成由聚氧乙烯脱水山梨醇单硬脂酸酯（吐温61），胆固醇和磷酸二鲸蜡酯组成的纳米粒子，用于封装研究。使用羧基荧光素染料（CF）作为药物模型来证明递送。监测niosomes中的染料量，囊泡浓度及每5分钟增加暴露于超声波后的平均粒径。发现特定频率的超声波可以可逆地渗透niosomes的脂质膜，以允许化合物的受控释放而不破坏niosome结构。

2. 发明授权

US07067726B2 Commercial production of recombinant manganese-dependent peroxidase in plants 有权
标题翻译：在植物中商业生产重组锰依赖性过氧化物酶
公开(公告)号：US07067726B2
公开(公告)日：2006-06-27
申请号：US10455915
申请日：2003-06-06
申请人： Elizabeth Hood , John Howard , Richard Clough , Kameshwari Pappu
发明人： Elizabeth Hood , John Howard , Richard Clough , Kameshwari Pappu
IPC分类号： A01H5/00 , C12N15/82 , C12N15/87 , C12N5/02 , C07H21/04
CPC分类号： C12N9/0065 , C12N15/8257 , C12Y111/01013
摘要： Expression of a recombinant manganese-dependent peroxidase in plants is described. Methods for the recovery of transgenic corn seeds that express high levels of functional manganese-dependent peroxidase are also provided. Manganese-dependent peroxidase vectors are engineered to contain sequences directing seed-preferred expression as well as sequences for cell wall-targeted localization.
摘要翻译：描述了重组锰依赖性过氧化物酶在植物中的表达。还提供了回收表达高水平功能性锰依赖性过氧化物酶的转基因玉米种子的方法。锰依赖性过氧化物酶载体被工程化以含有指导种子优选表达的序列以及用于细胞壁靶向定位的序列。

3. 发明授权

US07049484B2 Commercial production of proteases in plants 失效
公开(公告)号：US07049484B2
公开(公告)日：2006-05-23
申请号：US10654823
申请日：2003-09-04
申请人： John A. Howard , Elizabeth Hood
发明人： John A. Howard , Elizabeth Hood
IPC分类号： C12N5/04 , C12N15/57 , C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N9/6427 , C12N15/8257
摘要： Production of proteases in plants is set forth, whereby heterologous DNA encoding the protease is introduced into the plant and expression of the protein achieved. By such methods, expression is achieved in plants wherein the plant cell is not damaged, the protein can be recovered without contamination by other proteases, and can be expressed at levels such that commercial production of the enzyme is obtained. Expression levels can be at 0.1% of total soluble protein of the plant, or higher.

4. 发明申请

US20060015971A1 Commerical production of proteases in plants 审中-公开
标题翻译：植物中商业化生产蛋白酶
公开(公告)号：US20060015971A1
公开(公告)日：2006-01-19
申请号：US11226788
申请日：2005-09-14
申请人： John Howard , Elizabeth Hood
发明人： John Howard , Elizabeth Hood
IPC分类号： A01H1/00 , C12N15/82
CPC分类号： C12N9/6427 , C12N15/8257
摘要： Production of proteases in plants is set forth, whereby heterologous DNA encoding the protease is introduced into the plant and expression of the protein achieved. By such methods, expression is achieved in plants wherein the plant cell is not damaged, the protein can be recovered without contamination by other proteases, and can be expressed at levels such that commercial production of the enzyme is obtained. Expression levels can be at 0.1% of total soluble protein of the plant, or higher.
摘要翻译：阐述植物中蛋白酶的产生，由此将编码蛋白酶的异源DNA引入植物中并实现蛋白质的表达。通过这样的方法，在植物细胞未受损害的植物中实现表达，可以在不受其它蛋白酶污染的情况下回收蛋白质，并且可以以获得酶的商业生产的水平来表达。表达水平可以在植物总可溶性蛋白质的0.1％以上。

5. 发明授权

US07981442B2 Ultrasound enhancement of drug release across non-ionic surfactant membranes 有权
标题翻译：通过非离子表面活性剂膜超声增强药物释放
公开(公告)号：US07981442B2
公开(公告)日：2011-07-19
申请号：US11427034
申请日：2006-06-28
申请人： Elizabeth Hood , Joel A. Strom , Michael VanAuker
发明人： Elizabeth Hood , Joel A. Strom , Michael VanAuker
IPC分类号： A61K9/127 , A61K9/48
CPC分类号： A61K9/1272 , A61K9/0009 , A61K41/0028 , A61K49/0002
摘要： A method of targeted drug delivery and imaging using nonionic surfactant vesicles (niosomes) in combination with ultrasound. Niosomes have potential applications in targeted drug delivery and imaging because of their ability to encapsulate therapeutic agents and their enhanced uptake by physiological membranes. Ultrasound may be used to mediate delivery non-invasively by altering the niosome membrane structure. Niosomes composed of polyoxyethylene sorbitan monostearate (Tween 61), cholesterol, and dicetyl phosphate were synthesized via a thin film hydration technique and used for encapsulation studies. Carboxyfluorescein dye (CF) was used as a drug model to demonstrate delivery. The amount of dye in the niosomes, the concentration of the vesicles, and their mean particle size after each 5 minute incremental exposure to ultrasound was monitored. Dye concentration in niosome samples decreased while the population and size distribution of the niosome remained largely unchanged. Ultrasound is demonstrated to enhance the rate of dye diffusion across the niosome membrane non-destructively.
摘要翻译：使用非离子表面活性剂囊泡（niosomes）与超声波组合的靶向药物递送和成像的方法。 Niosomes由于其能够包封治疗剂和增强生理膜吸收的能力，在靶向药物递送和成像中具有潜在应用。超声波可用于通过改变niosome膜结构非侵入性地介导递送。通过薄膜水合技术合成由聚氧乙烯脱水山梨醇单硬脂酸酯（吐温61），胆固醇和磷酸二鲸蜡酯组成的纳米粒子，用于封装研究。使用羧基荧光素染料（CF）作为药物模型来证明递送。监测niosomes中的染料量，囊泡浓度及每5分钟增加暴露于超声波后的平均粒径。 niosome样品中的染料浓度下降，而niosome的人口和大小分布基本保持不变。证明超声可以非破坏性地提高染色体在染色体膜上的扩散速率。

6. 发明申请

US20060292211A1 Ultrasound Enhancement of Drug Release Across Non-Ionic Surfactant Membranes 有权
标题翻译：超声增强非离子表面活性剂膜上的药物释放
公开(公告)号：US20060292211A1
公开(公告)日：2006-12-28
申请号：US11427034
申请日：2006-06-28
申请人： Elizabeth Hood , Joel Strom , Michael VanAuker
发明人： Elizabeth Hood , Joel Strom , Michael VanAuker
IPC分类号： A61K9/127
CPC分类号： A61K9/1272 , A61K9/0009 , A61K41/0028 , A61K49/0002
摘要： A method of targeted drug delivery and imaging using nonionic surfactant vesicles (niosomes) in combination with ultrasound. Niosomes have potential applications in targeted drug delivery and imaging because of their ability to encapsulate therapeutic agents and their enhanced uptake by physiological membranes. Ultrasound may be used to mediate delivery non-invasively by altering the niosome membrane structure. Niosomes composed of polyoxyethylene sorbitan monostearate (Tween 61), cholesterol, and dicetyl phosphate were synthesized via a thin film hydration technique and used for encapsulation studies. Carboxyfluorescein dye (CF) was used as a drug model to demonstrate delivery. The amount of dye in the niosomes, the concentration of the vesicles, and their mean particle size after each 5 minute incremental exposure to ultrasound was monitored. Dye concentration in niosome samples decreased while the population and size distribution of the niosome remained largely unchanged. Ultrasound is demonstrated to enhance the rate of dye diffusion across the niosome membrane non-destructively.
摘要翻译：使用非离子表面活性剂囊泡（niosomes）与超声波组合的靶向药物递送和成像的方法。 Niosomes由于其能够包封治疗剂和增强生理膜吸收的能力，在靶向药物递送和成像中具有潜在应用。超声波可用于通过改变niosome膜结构非侵入性地介导递送。通过薄膜水合技术合成由聚氧乙烯脱水山梨醇单硬脂酸酯（吐温61），胆固醇和磷酸二鲸蜡酯组成的纳米粒子，用于封装研究。使用羧基荧光素染料（CF）作为药物模型来证明递送。监测niosomes中的染料量，囊泡浓度及每5分钟增加暴露于超声波后的平均粒径。 niosome样品中的染料浓度下降，而niosome的人口和大小分布基本保持不变。证明超声可以非破坏性地提高染色体在染色体膜上的扩散速率。

7. 发明授权

US6087558A Commercial production of proteases in plants 失效
标题翻译：在植物中商业化生产蛋白酶
公开(公告)号：US6087558A
公开(公告)日：2000-07-11
申请号：US120582
申请日：1998-07-22
申请人： John A. Howard , Elizabeth Hood
发明人： John A. Howard , Elizabeth Hood
IPC分类号： C12N9/76 , C12N15/55 , C12N15/82 , A01H5/10 , A01H5/00 , C12N5/04 , C12N15/12 , C12N15/57
CPC分类号： C12N9/6427 , C12N15/8257
摘要： Production of proteases in plants is set forth, whereby heterologous DNA encoding the protease is introduced into the plant and expression of the protein achieved. By such methods, expression is achieved in plants wherein the plant cell is not damaged, the protein can be recovered without contamination by other proteases, and can be expressed at levels such that commercial production of the enzyme is obtained. Expression levels can be at 0.1% of total soluble protein of the plant, or higher.
摘要翻译：阐述植物中蛋白酶的产生，由此将编码蛋白酶的异源DNA引入植物中并实现蛋白质的表达。通过这样的方法，在植物细胞未受损害的植物中实现表达，可以在不受其它蛋白酶污染的情况下回收蛋白质，并且可以以获得酶的商业生产的水平来表达。表达水平可以在植物总可溶性蛋白质的0.1％以上。

8. 发明申请

US20130156792A9 Uniform field magnetization and targeting of therapeutic formulations 有权
公开(公告)号：US20130156792A9
公开(公告)日：2013-06-20
申请号：US12653465
申请日：2009-12-15
申请人： Robert J. Levy , Michael Chorny , Vladimir Muzykantov , Elizabeth Hood
发明人： Robert J. Levy , Michael Chorny , Vladimir Muzykantov , Elizabeth Hood
IPC分类号： A61K39/44 , A61F2/82 , C12N13/00 , A61K38/44 , A61P43/00 , A61N2/10 , A61M31/00
CPC分类号： A61K9/5094 , A61K9/5115 , A61K9/5123 , A61K9/5146 , A61K9/5153 , A61K9/5169 , A61K9/5192 , A61K47/42 , A61K47/59 , A61K47/645 , A61K47/6455 , A61K47/6849 , A61K47/6921 , A61K47/6937 , A61K48/0008 , A61K48/0041 , A61K48/0083 , A61L31/16 , A61L2300/416 , A61L2300/60 , A61L2300/624 , C12N15/111 , C12N15/86 , C12N2320/32 , C12N2710/10343
摘要： Systems and methods for magnetic targeting of therapeutic particles are provided. Therapeutic particles comprise one or more magnetic or magnetizable materials and at least one therapeutic agent. Therapeutic particles are specifically targeted using uniform magnetic fields capable of magnetizing magnetizable materials, and can be targeted to particular locations in the body, or can be targeted for capture, containment, and removal. Therapeutic particles can comprise antioxidant enzymes, and can be targeted to cells to protect the cells from oxidative damage.

9. 发明申请

US20060026715A1 Commercial production of polysaccharide degrading enzymes in plants and methods of using same 有权
公开(公告)号：US20060026715A1
公开(公告)日：2006-02-02
申请号：US11219180
申请日：2005-09-02
申请人： Elizabeth Hood , John Howard
发明人： Elizabeth Hood , John Howard
IPC分类号： A01H1/00 , A01H5/00 , C12N9/24 , C12N5/04 , C12N15/82
CPC分类号： C12N15/8242 , C12N15/8246 , C12N15/8257
摘要： Expression of recombinant polysaccharide degrading enzymes in plants is described. In one embodiment, expression of the enzyme is preferentially directed to the seed of the plant. Expression may also be preferentially targeted to specific locations within the plant cell. Expression of cellulases in corn is shown. The result is the capacity to produce polysaccharide degrading enzymes in plants at commercially acceptable levels in a reliable manner. Methods of using same in production of ethanol is also described, including use of the plant-produced enzymes in the ethanol production process.

10. 发明授权

US06800792B1 Commercial production of laccase in plants 失效
标题翻译：在植物中商业生产漆酶
公开(公告)号：US06800792B1
公开(公告)日：2004-10-05
申请号：US09786960
申请日：2001-03-12
申请人： John A. Howard , Elizabeth Hood , Joseph Jilka
发明人： John A. Howard , Elizabeth Hood , Joseph Jilka
IPC分类号： C12N1509
CPC分类号： C12N9/0061 , C12N15/8205 , C12N15/8257
摘要： Expression of laccase in plants at commercial levels of production is provided. The laccase gene is preferably operably linked with promoter sequences preferentially directing expression of laccase to the seed of the plant, and may additionally include sequences directing expression to the plant cell wall. Methods of improving the process of introducing DNA into plants via Agrobacterium are also provided.
摘要翻译：提供漆酶在商业生产水平的植物中的表达。漆酶基因优选与优先引导漆酶表达的植物的启动子序列可操作地连接，并且还可以包括将表达指导到植物细胞壁的序列。还提供了通过农杆菌改进将DNA导入植物的过程的方法。

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