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1. 发明申请

US20120156167A1 ENHANCIN GENE BEL1 FROM BACILLUS THURINGIENSIS AND ITS USE IN BIOLOGICAL INSECTICIDE 有权
标题翻译：生物杀虫剂中的增强基因BEL1及其在生物杀虫剂中的用途
公开(公告)号：US20120156167A1
公开(公告)日：2012-06-21
申请号：US13133429
申请日：2009-11-23
申请人： Ming Sun , Ziniu Yu , Shangling Fang , Donghai Peng , Lifang Ruan , Shouwen Chen , Chunping Luo
发明人： Ming Sun , Ziniu Yu , Shangling Fang , Donghai Peng , Lifang Ruan , Shouwen Chen , Chunping Luo
IPC分类号： A01N37/46 , C07K14/32 , A01P15/00 , C07H21/04
CPC分类号： C12N15/8286 , A01N63/00 , A01N63/02 , C07K14/32 , C12N9/54 , Y02A40/162 , A01N2300/00
摘要： The isolation, cloning of an enhancin gene bel1 from Bacillus thuringiensis, and the use of an enhancin Bel1 in biological insecticides are disclosed in the present invention. A new enhancin gene bel1 is isolated from Bacillus thuringiensis subsp. kurstaki YBT-1520, and its encoded product is a new enhancin Bel1. The enhancin has very strong effect-enhancing activity on the insecticidal action of insecticidal crystal protein Cry1Ac in killing Lepidoptera insects. The insecticidal activity of genetically engineered Bacillus thuringiensis BMB0187 developed on the basis of this enhancin is 5.7 times as high as that of the initial strain.
摘要翻译：在本发明中公开了从苏云金芽孢杆菌中分离，克隆增强子基因bel1以及增强子Bel1在生物杀虫剂中的用途。从苏云金芽孢杆菌亚种分离出新的增强子基因bel1。 kurstaki YBT-1520，其编码产品是新的增效剂Bel1。增强子对杀虫水晶蛋白Cry1Ac杀死鳞翅目昆虫的杀虫作用具有非常强的增效作用。基于该增强子开发的遗传工程苏云金芽孢杆菌BMB0187的杀虫活性是初始菌株的杀虫活性的5.7倍。

2. 发明授权

US08623651B2 Enhancin gene bel1 from Bacillus thuringiensis and its use in biological insecticide 有权
标题翻译：来自苏云金芽孢杆菌的Enhancin基因bel1及其在生物杀虫剂中的应用
公开(公告)号：US08623651B2
公开(公告)日：2014-01-07
申请号：US13133429
申请日：2009-11-23
申请人： Ming Sun , Ziniu Yu , Shangling Fang , Donghai Peng , Lifang Ruan , Shouwen Chen , Chunping Luo
发明人： Ming Sun , Ziniu Yu , Shangling Fang , Donghai Peng , Lifang Ruan , Shouwen Chen , Chunping Luo
IPC分类号： C12N15/32 , C12N15/82 , C12N1/00 , C07K14/325
CPC分类号： C12N15/8286 , A01N63/00 , A01N63/02 , C07K14/32 , C12N9/54 , Y02A40/162 , A01N2300/00
摘要： The isolation, cloning of an enhancin gene bel1 from Bacillus thuringiensis, and the use of an enhancin Bel1 in biological insecticides are disclosed in the present invention. A new enhancin gene bel1 is isolated from Bacillus thuringiensis subsp. kurstaki YBT-1520, and its encoded product is a new enhancin Bel1. The enhancin has very strong effect-enhancing activity on the insecticidal action of insecticidal crystal protein Cry1Ac in killing Lepidoptera insects. The insecticidal activity of genetically engineered Bacillus thuringiensis BMB0187 developed on the basis of this enhancin is 5.7 times as high as that of the initial strain.
摘要翻译：在本发明中公开了从苏云金芽孢杆菌中分离，克隆增强子基因bel1以及增强子Bel1在生物杀虫剂中的用途。从苏云金芽孢杆菌亚种分离出新的增强子基因bel1。 kurstaki YBT-1520，其编码产品是新的增效剂Bel1。增强子对杀虫水晶蛋白Cry1Ac杀死鳞翅目昆虫的杀虫作用具有非常强的增效作用。基于该增强子开发的遗传工程苏云金芽孢杆菌BMB0187的杀虫活性是初始菌株的杀虫活性的5.7倍。

3. 发明申请

US20120015404A1 GENE CLUSTER FOR THURINGIENSIN SYNTHESIS 审中-公开
标题翻译：神经元合成基因簇
公开(公告)号：US20120015404A1
公开(公告)日：2012-01-19
申请号：US13145071
申请日：2009-12-15
申请人： Ming Sun , Ziniu Yu , Lifang Ruan , Shouwen Chen , Xiaoyan Liu , Donghai Peng
发明人： Ming Sun , Ziniu Yu , Lifang Ruan , Shouwen Chen , Xiaoyan Liu , Donghai Peng
IPC分类号： C12P19/44 , C12N1/20 , C12N15/61
CPC分类号： C12N15/52 , C07K14/32
摘要： The invention belongs to the technical field of genetic engineering of agricultural microorganisms. Specifically, the invention relates to producing and using gene cluster for thuringiensin synthesis from Bacillus thuringiensis. The gene cluster includes 11 genes of thuA, thuB, thuC, thuD, thuE, thuF, thuG, thu1, thu2, thu3 and thu4. These genes have the nucleotide sequence as shown in SEQ ID NO: 1. And the thuringiensin biosynthetase encoded by these genes have the amino acid sequences as shown in SEQ ID NO: 2-12. The genes according to the invention and the proteins encoded by those genes can be used as new nucleosides lead compound of pesticide library and provide new target for developing insecticides. In practical use, a series of new, efficient and low toxic insecticide can be obtained by structure modification.
摘要翻译：本发明属于农业微生物遗传工程技术领域。具体地说，本发明涉及生产和使用苏云金芽孢杆菌的苏氨酸素合成基因簇。基因簇包含thuA，thuB，thuC，thuD，thuE，thuF，thuG，thu1，thu2，thu3和thu4等11个基因。这些基因具有如SEQ ID NO：1所示的核苷酸序列。由这些基因编码的苏云金素生物合成酶具有如SEQ ID NO：2-12所示的氨基酸序列。根据本发明的基因和由这些基因编码的蛋白质可以用作农药图谱的新核苷酸铅化合物，并提供开发杀虫剂的新靶标。在实际应用中，通过结构修饰可以获得一系列新型，高效，低毒的杀虫剂。

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