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1. 发明授权

US10087482B2 Amplification of bisulfite-reacted nucleic acids 有权
公开(公告)号：US10087482B2
公开(公告)日：2018-10-02
申请号：US12865541
申请日：2009-02-06
申请人： Christian Korfhage , Dirk Loeffert , Ralf Peist , Nicolas Rudinger
发明人： Christian Korfhage , Dirk Loeffert , Ralf Peist , Nicolas Rudinger
IPC分类号： C12Q1/68 , C12Q1/6844
摘要： The present invention relates to a reaction mixture for the amplification of nucleic acids, the non-methylated cytosine bases of which have been converted to uracil bases by means of a bisulfition reaction. The invention also discloses methods for amplifying bisulfited nucleic acid and for determining the nucleic acid methylation state, and also kits based on the reaction mixture according to the invention.

2. 发明申请

US20120202198A1 PROCESS FOR THE SYNTHESIS OF A CDNA IN A SAMPLE IN AN ENZYMATIC REACTION 有权
标题翻译：在一个酶反应中样品中合成CDNA的方法
公开(公告)号：US20120202198A1
公开(公告)日：2012-08-09
申请号：US12377457
申请日：2007-08-13
申请人： Holger Engel , Subrahmanyam Yerramilli , Martin Kreutz , Dirk Loeffert , Christian Korfhage
发明人： Holger Engel , Subrahmanyam Yerramilli , Martin Kreutz , Dirk Loeffert , Christian Korfhage
IPC分类号： C12P19/34 , C12N9/12 , C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6848 , C12Q2527/101 , C12Q2521/131 , C12Q2521/107
摘要： This invention relates to a process for synthesis of a cDNA in a sample, in an enzymatic reaction, whereby the process comprises the steps: simultaneous preparation of a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, a buffer, at least one ribonucleotide, at least one deoxyribonucleotide, an anchor oligonucleotide; addition of a sample that comprises a ribonucleic acid; and incubation of the agents of the previous steps in one or more temperature steps, which are selected such that the first enzyme and the second enzyme show activity. The invention further relates to a reaction mixture that comprises a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, optionally a buffer, optionally at least one ribonucleotide, optionally at least one deoxyribonucleotide, and optionally an anchor oligonucleotide. Moreover, the invention relates to a kit that comprises a corresponding reaction mixture.
摘要翻译：本发明涉及在酶反应中合成样品中cDNA的方法，其中该方法包括以下步骤：同时制备具有多聚腺苷酸化活性的第一种酶，具有逆转录酶活性的第二种酶，缓冲液，至少一个核糖核苷酸，至少一个脱氧核糖核苷酸，锚定寡核苷酸; 加入包含核糖核酸的样品; 以及先前步骤的试剂在一个或多个温度步骤中的温育，其被选择为使得第一酶和第二酶显示活性。本发明还涉及包含具有多聚腺苷酸化活性的第一种酶，具有逆转录酶活性的第二种酶，任选的缓冲液，任选的至少一种核糖核苷酸，任选的至少一种脱氧核糖核苷酸和任选的锚定寡核苷酸的反应混合物。此外，本发明涉及包含相应反应混合物的试剂盒。

3. 发明授权

US08486628B2 Method of normalized quantification of nucleic acids using anchor oligonucleotides and adapter oligonucleotides 失效
标题翻译：使用锚定寡核苷酸和衔接子寡核苷酸对核酸进行归一化定量的方法
公开(公告)号：US08486628B2
公开(公告)日：2013-07-16
申请号：US13388224
申请日：2010-07-30
申请人： Dirk Loeffert , Christian Korfhage , Holger Engel
发明人： Dirk Loeffert , Christian Korfhage , Holger Engel
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6851 , C12Q2545/101 , C12Q2525/173 , C12Q2563/179 , C12Q2561/101
摘要： The present invention is related to normalized quantification of nucleic acids and to the normalization of quantities of nucleic acids in samples, e.g. mixtures of nucleic acids. The present invention relates to method for the normalization of the quantity of a nucleic acid to be quantified in a sample to the total quantity of nucleic acid in the sample; or to the total quantity of a specific class of nucleic acid in the sample.
摘要翻译：本发明涉及核酸的归一化定量和样品中核酸量的归一化，例如，核酸混合物。本发明涉及将样品中待定量的核酸的量归一化为样品中核酸总量的方法; 或相对于样品中特定类别的核酸的总量。

4. 发明申请

US20110053143A1 METHODS AND KITS FOR DETERMINING VIRAL LOAD IN CLINICAL SAMPLES 审中-公开
标题翻译：用于确定临床样本中病毒载量的方法和工具
公开(公告)号：US20110053143A1
公开(公告)日：2011-03-03
申请号：US12875533
申请日：2010-09-03
申请人： Brian Lowe , Dominic O'Neil , Irina Nazarenko , Dirk Loeffert
发明人： Brian Lowe , Dominic O'Neil , Irina Nazarenko , Dirk Loeffert
IPC分类号： C12Q1/70
CPC分类号： C12Q1/708
摘要： Methods and kits for determining load of an infectious agent in a sample are described, comprising performing at least one hybridization assay and calculating the load of the infectious agent in the sample from a detected nucleic acid. In particular, the methods and kits disclosed determine the load of human papillomavirus (HPV) in a sample.
摘要翻译：描述了用于确定样品中感染剂负荷的方法和试剂盒，包括进行至少一种杂交测定并从检测到的核酸计算样品中感染剂的载量。特别地，所公开的方法和试剂盒确定样品中人乳头瘤病毒（HPV）的负荷。

5. 发明授权

US09238809B2 Compositions, methods, and kits for isolating and analyzing nucleic acids using an anion exchange material 有权
标题翻译：使用阴离子交换材料分离和分析核酸的组合物，方法和试剂盒
公开(公告)号：US09238809B2
公开(公告)日：2016-01-19
申请号：US12851311
申请日：2010-08-05
申请人： Yuri Khripin , Dirk Loeffert , Roland Fabis , Nadine Krueger
发明人： Yuri Khripin , Dirk Loeffert , Roland Fabis , Nadine Krueger
IPC分类号： C12Q1/68 , C07H23/00 , C12N15/10
CPC分类号： C12N15/101 , Y10T436/143333
摘要： The present disclosure relates to methods for isolating, amplifying, and/or analyzing nucleic acids in the presence of an anion exchange material by performing the isolation, amplification and/or analysis step in the presence of at least one anionic compound.
摘要翻译：本公开涉及通过在至少一种阴离子化合物的存在下进行分离，扩增和/或分析步骤在阴离子交换材料存在下分离，扩增和/或分析核酸的方法。

6. 发明授权

US09217173B2 Method of polyadenylation and CDNA synthesis in a single reaction 有权
标题翻译：单次反应中聚腺苷酸化和CDNA合成的方法
公开(公告)号：US09217173B2
公开(公告)日：2015-12-22
申请号：US12377457
申请日：2007-08-13
申请人： Holger Engel , Subrahmanyam Yerramilli , Martin Kreutz , Dirk Loeffert , Christian Korfhage
发明人： Holger Engel , Subrahmanyam Yerramilli , Martin Kreutz , Dirk Loeffert , Christian Korfhage
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6848 , C12Q2527/101 , C12Q2521/131 , C12Q2521/107
摘要： This invention relates to a process for synthesis of a cDNA in a sample, in an enzymatic reaction, wherein the process comprises the steps: simultaneous preparation of a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, a buffer, at least one ribonucleotide, at least one deoxyribonucleotide, an anchor oligonucleotide; addition of a sample that comprises a ribonucleic acid; and incubation of the agents of the previous steps in one or more temperature steps, which are selected such that the first enzyme and the second enzyme show activity. The invention further relates to a reaction mixture that comprises a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, optionally a buffer, optionally at least one ribonucleotide, optionally at least one deoxyribonucleotide, and optionally an anchor oligonucleotide. Moreover, the invention relates to a kit that comprises a corresponding reaction mixture.
摘要翻译：本发明涉及一种在酶促反应中合成样品中cDNA的方法，其中该方法包括以下步骤：同时制备具有多聚腺苷酸化活性的第一种酶，具有逆转录酶活性的第二种酶，至少缓冲液一个核糖核苷酸，至少一个脱氧核糖核苷酸，锚定寡核苷酸; 添加包含核糖核酸的样品; 以及先前步骤的试剂在一个或多个温度步骤中的温育，其被选择为使得第一酶和第二酶显示活性。本发明还涉及包含具有多聚腺苷酸化活性的第一种酶，具有逆转录酶活性的第二种酶，任选的缓冲液，任选的至少一种核糖核苷酸，任选的至少一种脱氧核糖核苷酸和任选的锚定寡核苷酸的反应混合物。此外，本发明涉及包含相应反应混合物的试剂盒。

7. 发明申请

US20120190026A1 METHOD OF NORMALIZED QUANTIFICATION OF RNA 审中-公开
标题翻译： RNA的正规化定量方法
公开(公告)号：US20120190026A1
公开(公告)日：2012-07-26
申请号：US13387974
申请日：2010-07-30
申请人： Dirk Loeffert , Christian Korfhage , Holger Engel
发明人： Dirk Loeffert , Christian Korfhage , Holger Engel
IPC分类号： C12Q1/68 , G01N21/64
CPC分类号： C12Q1/6851 , C12Q2525/173 , C12Q2561/101 , C12Q2545/101 , C12Q2565/101 , C12Q2563/173 , C12Q2521/107
摘要： The present invention is related to normalized quantification of RNAs and to the normalization of quantities of RNAs in samples, e.g. mixtures of RNAs. The present invention relates to method for the normalization of the quantity of a RNA to be quantified in a sample to the total quantity of RNA in the sample; or to the total quantity of a specific class of RNA in the sample.
摘要翻译：本发明涉及RNA的归一化定量和样品中RNA的量的归一化。 RNA的混合物本发明涉及将样品中待定量的RNA的量归一化为样品中RNA总量的方法; 或样品中特定类别的RNA的总量。

8. 发明申请

US20100285473A1 THERMOPHILIC HELICASE DEPENDENT AMPLIFICATION TECHNOLOGY WITH ENDPOINT HOMOGENOUS FLUORESCENT DETECTION 审中-公开
标题翻译：具有端点均质荧光检测的热致命性相关放大技术
公开(公告)号：US20100285473A1
公开(公告)日：2010-11-11
申请号：US12695001
申请日：2010-01-27
申请人： John Wolff , Victoria Doseeva , Thomas Forbes , Gwynne Roth , Irina Nazarenko , Dirk Loeffert
发明人： John Wolff , Victoria Doseeva , Thomas Forbes , Gwynne Roth , Irina Nazarenko , Dirk Loeffert
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/686 , C12Q1/6844 , C12Q1/6865 , C12Q2563/131 , C12Q2527/137 , C12Q2521/513
摘要： Disclosed herein are methods of amplifying a target nucleic acid in a helicase-dependent reaction. Also disclosed are methods of amplifying and detecting a target nucleic acid in a helicase-dependent reaction as well as modified detection labels to assist in the detection.
摘要翻译：本文公开了在解旋酶依赖性反应中扩增靶核酸的方法。还公开了在解旋酶依赖性反应中扩增和检测靶核酸的方法以及用于辅助检测的经修改的检测标记。

9. 发明申请

US20120252014A1 METHOD OF NORMALIZED QUANTIFICATION OF NUCLEIC ACIDS USING ANCHOR OLIGONUCLEOTIDES AND ADAPTER OLIGONUCLEOTIDES 失效
标题翻译：使用锚定寡核苷酸和适配子寡核苷酸对核酸进行正规化定量的方法
公开(公告)号：US20120252014A1
公开(公告)日：2012-10-04
申请号：US13388224
申请日：2010-07-30
申请人： Dirk Loeffert , Christian Korfhage , Holger Engel
发明人： Dirk Loeffert , Christian Korfhage , Holger Engel
IPC分类号： G01N21/64
CPC分类号： C12Q1/6851 , C12Q2545/101 , C12Q2525/173 , C12Q2563/179 , C12Q2561/101
摘要： The present invention is related to normalized quantification of nucleic acids and to the normalization of quantities of nucleic acids in samples, e.g. mixtures of nucleic acids. The present invention relates to method for the normalization of the quantity of a nucleic acid to be quantified in a sample to the total quantity of nucleic acid in the sample; or to the total quantity of a specific class of nucleic acid in the sample.
摘要翻译：本发明涉及核酸的归一化定量和样品中核酸量的归一化，例如，核酸混合物。本发明涉及将样品中待定量的核酸的量归一化为样品中核酸总量的方法; 或相对于样品中特定类别的核酸的总量。

10. 发明申请

US20120190027A1 LIGATION-BASED METHOD OF NORMALIZED QUANTIFICATION OF NUCLEIC ACIDS 审中-公开
标题翻译：基于LIGATION的核酸正规化定量方法
公开(公告)号：US20120190027A1
公开(公告)日：2012-07-26
申请号：US13388228
申请日：2010-07-30
申请人： Dirk Loeffert , Christian Korfhage , Holger Engel
发明人： Dirk Loeffert , Christian Korfhage , Holger Engel
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q2561/125 , C12Q2525/173
摘要： The present invention is related to normalized quantification of nucleic acids and to the normalization of quantities of nucleic acids in samples, e.g. mixtures of nucleic acids. The present invention relates to method for the normalization of the quantity of a nucleic acid to be quantified in a sample to the total quantity of nucleic acid in the sample; or to the total quantity of a specific class of nucleic acid in the sample.
摘要翻译：本发明涉及核酸的归一化定量和样品中核酸量的归一化，例如，核酸混合物。本发明涉及将样品中待定量的核酸的量归一化为样品中核酸总量的方法; 或相对于样品中特定类别的核酸的总量。

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