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1. 发明授权

US06475756B1 Development of viruses resistant to inactivation by the human complement system 失效
标题翻译：开发抗人类补体系统灭活的病毒
公开(公告)号：US06475756B1
公开(公告)日：2002-11-05
申请号：US09554838
申请日：2000-07-21
申请人： Dagmar Wirth , Dirk Spitzer , Hansjoerg Hauser
发明人： Dagmar Wirth , Dirk Spitzer , Hansjoerg Hauser
IPC分类号： C12P2104
CPC分类号： C07K14/005 , A61K48/00 , C07K14/70596 , C07K2319/00 , C12N15/86 , C12N2740/13022 , C12N2740/13043 , C12N2740/13045 , C12N2740/13052 , C12N2810/60 , C12N2810/855
摘要： Murine retroviruses are the most important transfer systems for human gene therapy. However, their application is currently limited. One of the major restrictions both for an application in vivo resides in the problem that this virus type is sensitive to inactivation by human complement factors. Our invention overcomes this limitation. We have modified murine recombinant retroviruses in a way that they are resistant to human complement factors. This was achieved by genetic modification of the retroviral surface protein env which is responsible for receptor interaction: the receptor interacting domain of env was fused to catalytically active domains of human complement inactivation factors. These modified env were expressed in complement-sensitive cells and specifically integrated into virus particles. By this strategy cells and viruses are generated that are fully resistant to complement attack. Thus, this strategy provides a tool for establishment of complement resistant cells and generation of viruses for in vivo gene therapy.
摘要翻译：鼠逆转录病毒是人类基因治疗最重要的转移系统。但是，它们的应用目前是有限的。在体内应用的主要限制之一在于这种病毒类型对人补体因子的失活敏感的问题。我们的发明克服了这个限制。我们已经修改了鼠重组逆转录病毒，使其具有抗人补体因子的作用。这是通过负责受体相互作用的逆转录病毒表面蛋白env的遗传修饰实现的：env的受体相互作用结构域融合到人补体失活因子的催化活性结构域。这些修饰的env在补体敏感细胞中表达并且特异性整合到病毒颗粒中。通过这种策略，产生完全抵抗补体攻击的细胞和病毒。因此，该策略提供了建立补体抗性细胞和产生用于体内基因治疗的病毒的工具。

2. 发明授权

US5211957A Solid rapidly disintegrating dosage form 失效
标题翻译：固体快速崩解剂型
公开(公告)号：US5211957A
公开(公告)日：1993-05-18
申请号：US915050
申请日：1992-07-16
申请人： Ruth Hagemann , Dagmar Wirth , Henri-Julien Bronner
发明人： Ruth Hagemann , Dagmar Wirth , Henri-Julien Bronner
IPC分类号： A61K9/20 , A61K9/46 , A61K9/50 , A61K31/195
CPC分类号： A61K9/0007 , A61K31/195 , A61K9/2081 , A61K9/5005
摘要： The invention relates to a solid, rapidly disintegrating dosage form in the form of effervescent tablets for producing an aqueous suspension of diclofenac for peroral administration. The dosage form contains diclofenac in micronised form provided with a permeable, swellable coating, together with pharmaceutical excipients.
摘要翻译：本发明涉及用于产生用于口服给药的双氯芬酸水性悬浮液的泡腾片剂形式的固体快速崩解剂型。剂型包含微粉化形式的双氯芬酸，其具有可渗透的可溶胀涂层以及药用赋形剂。

3. 发明授权

US5096717A Double-coated granules of disodium pamidronate 失效
标题翻译：二氨基甲酸二丁酯的双层糖浆
公开(公告)号：US5096717A
公开(公告)日：1992-03-17
申请号：US577420
申请日：1990-09-04
申请人： Dagmar Wirth , Christian Bucher
发明人： Dagmar Wirth , Christian Bucher
IPC分类号： A61K9/58 , A61K9/50 , A61K9/52 , A61K9/54 , A61K9/62 , A61K31/66 , A61P43/00
CPC分类号： A61K31/66 , A61K9/5073
摘要： The invention relates to an advantageous oral administration from the disodium pamidronate in capsules. These capsules are filled with double-coated granules. The inner coating is hydrophilic and elastic, and the outer coating is gastric juice-resistant and intestinal juice-soluble. The granules are distinguished by good gastric compatibility.
摘要翻译：本发明涉及从胶囊中的帕米膦酸二钠的口服给药。这些胶囊填充有双重包衣的颗粒。内涂层具有亲水性和弹性，外涂层耐胃液和肠液可溶。颗粒的胃相容性好。

4. 发明申请

US20110189142A1 Senescene Cells and Methods For Its Production 审中-公开
标题翻译：新世纪细胞及其生产方法
公开(公告)号：US20110189142A1
公开(公告)日：2011-08-04
申请号：US12999184
申请日：2009-07-03
申请人： Tobias May , Dagmar Wirth , Hansjoerg Hauser
发明人： Tobias May , Dagmar Wirth , Hansjoerg Hauser
IPC分类号： A61K35/12 , C12N15/79 , C12N15/87 , C12N5/10 , C12P21/00 , G01N33/53 , C12Q1/68 , C12Q1/02 , C12Q1/34
CPC分类号： C12N15/86 , C07K14/4705 , C07K14/82 , C12N9/1241 , C12N2740/16043 , C12N2830/003 , C12N2840/203
摘要： The present invention relates to a method for the generation of immortalized transduced cell lines susceptible to senescence whereby the senescence is exogenously inducible and said cells can switch severalfold between the senescent stage and the immortalized stage. In particular, the present invention relates to a method for the generation of immortalized transduced cell lines susceptible to senescence wherein two or more different immortalizing gene sequences have been incorporated whereby said immortalizing gene sequences are regulated by regulators controlled via exogenous means. In a further aspect, the present invention relates to immortalized transduced cell lines obtainable with said method. In addition, methods for screening molecules influencing senescence of cells are provided as well as kits for conducting the same.
摘要翻译：本发明涉及一种用于产生易衰老的永生转导细胞系的方法，其中衰老是外源诱导型的，并且所述细胞可以在衰老阶段和永生化阶段之间切换几倍。特别地，本发明涉及一种用于产生易衰老敏化的永生化转导细胞系的方法，其中已经并入两种或更多种不同的永生化基因序列，由此所述永生化基因序列由通过外源手段控制的调节子调节。在另一方面，本发明涉及可用所述方法获得的永生转导的细胞系。此外，还提供了筛选影响细胞衰老的分子的方法以及用于进行细胞衰老的试剂盒。

5. 发明申请

US20080124304A1 Method for the Generation of Virus Producing Cell Lines and Cell Lines 审中-公开
标题翻译：产生细胞系和细胞系病毒的方法
公开(公告)号：US20080124304A1
公开(公告)日：2008-05-29
申请号：US11718385
申请日：2005-11-01
申请人： Dagmar Wirth , Hansjoerg Hauser , Roland Schucht
发明人： Dagmar Wirth , Hansjoerg Hauser , Roland Schucht
IPC分类号： A61K35/76 , C12N15/87 , C12N5/06 , A61P43/00 , C12N7/00
CPC分类号： C12N15/86 , C12N2740/13043 , C12N2800/30
摘要： The present invention relates to a method for generating virus producing cell lines and master producer cell lines. In particular, the present invention relates to methods allowing the production of virus producing cell lines without conducting laborious selection work. Moreover, the present invention pertains to virus producing cell lines obtained with the methods according to the present invention as well as to virus obtained using said cell lines.
摘要翻译：本发明涉及产生病毒的细胞系和主生产细胞系的方法。特别地，本发明涉及允许生产病毒产生细胞系而不进行艰苦选择工作的方法。此外，本发明涉及通过本发明的方法获得的病毒产生细胞系以及使用所述细胞系获得的病毒。

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