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1. 发明授权

US06762039B2 Bacillus subtillis with an inactivated cysteine protease-1 有权
标题翻译：具有灭活的半胱氨酸蛋白酶-1的枯草芽孢杆菌
公开(公告)号：US06762039B2
公开(公告)日：2004-07-13
申请号：US09462846
申请日：2000-01-13
申请人： David A. Estell
发明人： David A. Estell
IPC分类号： C12P2104
CPC分类号： C12N9/54 , C11D3/386 , C12N9/90
摘要： The present invention relates to the identification of novel cysteine proteases in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the Bacillus subtilis cysteine proteases CP1, CP2 and CP3. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding CP1, CP2 or CP3. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a cysteine protease of the present invention.
摘要翻译：本发明涉及革兰氏阳性微生物中新型半胱氨酸蛋白酶的鉴定。本发明提供枯草芽孢杆菌半胱氨酸蛋白酶CP1，CP2和CP3的核酸和氨基酸序列。本发明还提供具有编码CP1，CP2或CP3的基因的部分或全部的突变或缺失的宿主细胞。本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。本发明还提供了包含本发明的半胱氨酸蛋白酶的清洁组合物。

2. 发明授权

US06716607B1 Chicken interferon gene and novel recombinant DNA 失效
标题翻译：鸡干扰素基因和新型重组DNA
公开(公告)号：US06716607B1
公开(公告)日：2004-04-06
申请号：US09495491
申请日：2000-02-01
申请人： Margaret J. Sekellick , Philip I. Marcus , Anthony F. Ferrandino
发明人： Margaret J. Sekellick , Philip I. Marcus , Anthony F. Ferrandino
IPC分类号： C12P2104
CPC分类号： C12N15/8509 , A01K2217/05 , A01K2227/30 , A01K2267/02 , A61K38/00 , C07K14/555 , C12N2799/027
摘要： A chicken interferon gene coding for the amino acid sequence of SEQ ID NO:1 is disclosed. There is also disclosed a method of producing chicken interferon recombinantly, a method of isolating other non-mammalian interferon genes, a method of making a transgenic fowl having the chicken interferon gene incorporated therein, and method for delivery of the chicken interferon in the bird, such as by genetic immunization or aerosol. Expression of the gene coding for the amino acid sequence of SEQ ID NO:1 yields functional chicken interferon.
摘要翻译：公开了编码SEQ ID NO：1的氨基酸序列的鸡干扰素基因。还公开了重组生产鸡干扰素的方法，分离其他非哺乳动物干扰素基因的方法，其中掺入鸡干扰素基因的转基因家禽的制备方法，以及鸟类中的鸡干扰素的输送方法，例如通过基因免疫或气溶胶。编码SEQ ID NO：1的氨基酸序列的基因的表达产生功能性鸡干扰素。

3. 发明授权

US06696276B2 Vasopermeability-enhancing conjugates 失效
标题翻译：血管通透性增强共轭物
公开(公告)号：US06696276B2
公开(公告)日：2004-02-24
申请号：US10342426
申请日：2003-01-13
申请人： Alan L. Epstein , Michael Glovsky
发明人： Alan L. Epstein , Michael Glovsky
IPC分类号： C12P2104
CPC分类号： A61K51/1045 , A61K47/56 , A61K47/6851 , A61K2121/00
摘要： Liposomal conjugates having a clinically useful delivery vehicle linked to a biologically active species which acts to increase vascular permeability and expand blood volume at or in proximity to the tumor site are disclosed. The vehicle-linked species may be, for example, a vasoactive agent, a substance that recruits or amplifies a vasoactive species, a drug, or a pharmaceutical compound. Suitable biological species comprises peptides, lipids, carbohydrates, or their derivatives. Chemical or recombinant DNA methods suitable for linking the species to the vehicles are indicated. A therapy is disclosed which comprises administering the vasoactive conjugate and delivering a diagnostic agent or a therapeutic agent at an optimal time thereafter, when tumor vasculature is maximally affected.
摘要翻译：公开了具有连接到生物活性物质的临床有用的递送载体的脂质体缀合物，其用于增加血管通透性并扩大肿瘤部位处或附近的血容量。载体连接的物种可以是例如血管活性剂，募集或放大血管活性物质的物质，药物或药物化合物。合适的生物物种包括肽，脂质，碳水化合物或其衍生物。表明适用于将物种与载体连接的化学或重组DNA方法。公开了一种疗法，其包括施用血管活性缀合物并在其后最佳时间递送诊断剂或治疗剂，当肿瘤脉管系统受到最大影响时。

4. 发明授权

US06677138B2 Production of a multimeric protein by cell fusion method 有权
标题翻译：通过细胞融合法制备多聚体蛋白
公开(公告)号：US06677138B2
公开(公告)日：2004-01-13
申请号：US09945387
申请日：2001-08-30
申请人： Nobuaki Hori , Claude Geoffrey Davis , Krisztina M. Zsebo , Aya Jakobovits
发明人： Nobuaki Hori , Claude Geoffrey Davis , Krisztina M. Zsebo , Aya Jakobovits
IPC分类号： C12P2104
CPC分类号： C07K14/00 , C07K16/00 , C07K16/244 , C07K2317/21 , C12N5/16 , C12N5/166 , C12N15/62 , C12N2510/02
摘要： The present invention features a method of producing a multimeric protein from a hybrid cell formed from the fusion of two or more cells, each of which cell is engineered to express one component of the multimeric protein, as well as a method for screening for successful fusion of the cells to produce a desired hybrid cell. The methods of the invention are widely applicable to the production of proteins having two or more components.
摘要翻译：本发明的特征在于从由两个或多个细胞的融合形成的杂交细胞产生多聚体蛋白质的方法，每个细胞被工程化以表达多聚体蛋白质的一个组分，以及筛选成功融合的方法的细胞以产生所需的杂交细胞。本发明的方法可广泛用于生产具有两种或多种组分的蛋白质。

5. 发明授权

US06653104B2 Immunotoxins, comprising an internalizing antibody, directed against malignant and normal cells 失效
公开(公告)号：US06653104B2
公开(公告)日：2003-11-25
申请号：US09986119
申请日：2001-11-07
申请人： David M. Goldenberg
发明人： David M. Goldenberg
IPC分类号： C12P2104
CPC分类号： C07K14/5443 , A61K38/00 , A61K47/642 , A61K47/6829 , A61K47/6849 , A61K2039/505 , C07K14/463 , C07K14/7155 , C07K16/2803 , C07K16/2833 , C07K16/2896 , C07K16/3061 , C07K2317/622 , C07K2317/77 , C07K2319/00
摘要： The present invention relates to immunotoxins that effectively kill malignant cells having a given marker. The immunotoxins are reagents that comprise internalizing antibodies conjugated to cytotoxic ribonucleases or fragments thereof. The internalizing antibodies are capable of binding with a chosen tumor cell, and thereby confer little non-specific toxicity to the immunotoxin in a host. The immunotoxins exhibit up to 2000-fold higher toxicity against malignant B cells than did the ribonuclease counterparts alone.

6. 发明授权

US06613548B1 Fusion products containing insoluble proteinaceous tag 有权
标题翻译：融合产品含有不溶性蛋白质标签
公开(公告)号：US06613548B1
公开(公告)日：2003-09-02
申请号：US09744604
申请日：2001-01-24
申请人： Ruiyin Chu
发明人： Ruiyin Chu
IPC分类号： C12P2104
CPC分类号： C12N9/0046 , C07K14/005 , C07K14/705 , C07K2319/00 , C07K2319/715 , C07K2319/81 , C12N15/62 , C12N2710/14022
摘要： A process is disclosed for the preparation of a recombinantly expressed fusion product comprised of a proteinaceous tag and a soluble protein of interest and the separation of the fusion product from the host cell in which it is expressed. The tag and in turn the fusion product is insoluble in the host cell lysate solution and the fusion product is separated therefrom by centrifugation or filtration.
摘要翻译：公开了一种用于制备由蛋白质标签和感兴趣的可溶性蛋白质组成的重组表达的融合产物和从其表达的宿主细胞分离融合产物的方法。标签，然后融合产物不溶于宿主细胞裂解物溶液，并通过离心或过滤将融合产物分离。

7. 发明授权

US06558926B1 Method for production of tetanus toxin using media substantially free of animal products 有权
标题翻译：使用基本上不含动物产品的培养基生产破伤风毒素的方法
公开(公告)号：US06558926B1
公开(公告)日：2003-05-06
申请号：US09354959
申请日：1999-07-16
申请人： Arnold L. Demain , Aiqi Fang
发明人： Arnold L. Demain , Aiqi Fang
IPC分类号： C12P2104
CPC分类号： C12N1/20 , C07K14/33 , C12P21/02 , Y10S435/842
摘要： The present invention provides a system for the growth of C. tetani and production of Tetanus Toxin for use in formulating Tetanus Toxoid preparations. The system includes growth media that contain significantly reduced levels of meat or dairy by-products using non-animal based products to replace the animal-derived products. Preferred media are substantially free of animal-derived products.
摘要翻译：本发明提供了用于制备破伤风类毒素制剂的破伤风破伤风生长系统和破伤风毒素的生产系统。该系统包括使用非动物基产品来显着降低肉类或乳制品副产品水平以取代动物衍生产品的生长培养基。优选的培养基基本上不含动物来源的产品。

8. 发明授权

US06551799B2 Interleukin-22 polypeptides, nucleic acids encoding the same and methods for the treatment of pancreatic disorders 有权
公开(公告)号：US06551799B2
公开(公告)日：2003-04-22
申请号：US09870574
申请日：2001-05-30
申请人： Austin L. Gurney , Sudeepta Aggarwal , Ming-Hong Xie , Ellen M. Maruoka , Jessica S. Foster , Audrey Goddard , William I. Wood
发明人： Austin L. Gurney , Sudeepta Aggarwal , Ming-Hong Xie , Ellen M. Maruoka , Jessica S. Foster , Audrey Goddard , William I. Wood
IPC分类号： C12P2104
CPC分类号： C07K14/54 , A61K38/00 , C07K2319/30 , G01N2333/54 , G01N2500/10
摘要： The present invention is directed to interleukin-22 polypeptides and nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.

9. 发明授权

US06524825B1 Immune responses against HPV antigens elicited by compositions comprising an HPV antigen and a stress protein or an expression vector capable of expression of these proteins 失效
公开(公告)号：US06524825B1
公开(公告)日：2003-02-25
申请号：US09498918
申请日：2000-02-04
申请人： Lee A. Mizzen , N. Randall Chu , Huacheng Bill Wu
发明人： Lee A. Mizzen , N. Randall Chu , Huacheng Bill Wu
IPC分类号： C12P2104
CPC分类号： C07K14/005 , A61K39/12 , A61K39/385 , A61K47/646 , A61K2039/51 , A61K2039/5156 , A61K2039/5256 , A61K2039/55516 , A61K2039/57 , A61K2039/585 , A61K2039/60 , A61K2039/6043 , C07K14/35 , C07K2319/00 , C12N2710/20022 , C12N2710/20034
摘要： The present invention relates to compositions for inducing an immune response, preferably a cellular, in particular a cell-mediated, cytolytic immune response, to human papillomavirus (HPV) protein antigens displayed by HPV or exhibited by infected cells including cells from cervical and other tumors. In one embodiment, compositions comprise an HPV protein antigen joined to a stress protein (or heat shock protein (Hsp)). The HPV protein antigen may be joined to the stress protein by chemical conjugation or noncovalently using linking moieties, or the HPV protein antigen and the stress protein may be joined in a fusion protein containing both HPV protein antigen and stress protein sequences. In another embodiment, compositions comprise an expression vector including, in expressible form, sequences encoding the HPV protein antigen and sequences encoding the stress protein. The expression vector can be introduced into cells of a subject, or it can be used to transduce cells of the subject ex vivo, resulting in the expression of an HPV protein antigen-stress protein fusion protein that will stimulate the subject's immune response to the HPV protein antigen. The present invention also relates to compositions comprising a stress protein linked to an HPV antigen and another pharmacologically acceptable component, to stress protein-HPV protein antigen fusions and conjugates and to expression vectors encoding and capable of directing the expression in a subject's cells of a fusion protein comprising a stress protein and an HPV protein antigen sequence. The present invention also relates to uses of these compositions to induce immune responses against HPV and HPV protein antigen-exhibiting cells including HPV-associated tumors.

10. 发明授权

US06500648B1 Methods for recombinant peptide production 有权
标题翻译：重组肽生产方法
公开(公告)号：US06500648B1
公开(公告)日：2002-12-31
申请号：US09760397
申请日：2001-01-12
申请人： Marc D. Better , Patrick D. Gavit
发明人： Marc D. Better , Patrick D. Gavit
IPC分类号： C12P2104
CPC分类号： C12N15/62 , C07K14/4742 , C07K14/51 , C07K2319/00 , C07K2319/35 , C12N15/70
摘要： The present invention provides improved methods for the production of recombinant peptides from bacterial cells.
摘要翻译：本发明提供了用于从细菌细胞生产重组肽的改进方法。

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