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    • 7. 发明授权
    • Gamma-conopeptides
    • 伽玛肽
    • US06624288B1
    • 2003-09-23
    • US09210952
    • 1998-12-15
    • Michael FainzilberKarel S. KitsAlma L. BurlingameBaldomero M. OliveraCraig WalkerMaren WatkinsReshma ShettyLourdes J. CruzJulita ImperialClark Colledge
    • Michael FainzilberKarel S. KitsAlma L. BurlingameBaldomero M. OliveraCraig WalkerMaren WatkinsReshma ShettyLourdes J. CruzJulita ImperialClark Colledge
    • C07K500
    • C07K14/43504A61K38/00
    • This invention relates to relatively short peptides about 25-40 residues in length, which are naturally available in minute amounts in the venom of the cone snails or analogs to the naturally available peptides, and which include three cyclizing disulfide linkages and one or more &ggr;-carboxyglutamate residues. More specifically, the present invention is directed to &ggr;-conopeptides having the general formula I: Xaa1-Cys-Xaa2-Cys-Xaa3-Xaa4-Cys-Cys-Xaa5-Cys-Xaa6-Cys-Xaa7 (SEQ ID NO:1), as described herein; or having the general formula II: Xaa1-Cys-Xaa2-Cys-Xaa3-Xaa4-Cys-Cys-Xaa5-Xaa6-Cys-Xaa7-Cys-Xaa8 (SEQ ID NO:2), as defined herein; or having the general formula III: Xaa1-Cys-Xaa2-Cys-Xaa3-Xaa4-Xaa5-Cys-Cys-Ser-Asn-Ser-Cys-Asp-Xaa2-Cys-Xaa7 (SEQ ID NO:3), as described herein; or having the general formula IV: Xaa1-Cys-Xaa2-Cys-Xaa3-Xaa4-Xaa5-Cys-Cys-Ser-Asn-Ser-Cys-Asp-Xaa6-Cys-Xaa7 (SEQ ID NO:4), as described herein; or having the general formula V: Xaa1-Xaa2-Cys-Xaa3-Xaa4-Phe-Xaa5-Cys-Thr-Xaa6-Ser-Xaa7-Cys-Cys-Ser-Asn-Ser-Cys-Asp-Gln-Thr-Tyr-Cys-Xaa8-Leu-Xaa9 (SEQ ID NO:5), as described herein. The invention further relates to specific &ggr;-conopeptides, specific pro-&ggr;-conopeptides and nucleic acids encoding the pro-&ggr;-conopeptides. The invention also includes pharmaceutically acceptable salts of the conopeptides. These conopeptides are useful as agonists of neuronal pacemaker calcium channels.
    • 本发明涉及长度约25-40个残基的相对较短的肽,其在锥形蜗牛的毒液中或天然存在的肽的类似物中天然可用量少,并且其包括三个环化二硫键和一个或多个γ- 羧基谷氨酸残基。 更具体地,本发明涉及具有通式I的X射线衍生肽:Xaa1-Cys-Xaa2-Cys-Xaa3-Xaa4-Cys-Cys-Xaa5-Cys-Xaa6-Cys-Xaa7(SEQ ID NO:1) ,如本文所述; 或具有如本文所定义的通式II:Xaa1-Cys-Xaa2-Cys-Xaa3-Xaa4-Cys-Cys-Xaa5-Xaa6-Cys-Xaa7-Cys-Xaa8(SEQ ID NO:2) 或具有通式III:Xaa1-Cys-Xaa2-Cys-Xaa3-Xaa4-Xaa5-Cys-Cys-Ser-Asn-Ser-Cys-Asp-Xaa2-Cys-Xaa7(SEQ ID NO:3) 在这里 或具有通式IV:Xaa1-Cys-Xaa2-Cys-Xaa3-Xaa4-Xaa5-Cys-Cys-Ser-Asn-Ser-Cys-Asp-Xaa6-Cys-Xaa7(SEQ ID NO:4) 在这里 或具有通式V:Xaa1-Xaa2-Cys-Xaa3-Xaa4-Phe-Xaa5-Cys-Thr-Xaa6-Ser-Xaa7-Cys-Cys-Ser-Asn-Ser-Cys-Asp-Gln-Thr-Tyr -Cys-Xaa8-Leu-Xaa9(SEQ ID NO:5),如本文所述。 本发明进一步涉及具体的γ-配体肽,特异性前-γ-肽和编码前γ-多肽的核酸。 本发明还包括肽的药学上可接受的盐。 这些肽可用作神经元起搏器钙通道的激动剂。