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1. 发明申请

US20060210972A1 Annotation of genome sequences 审中-公开
标题翻译：基因组序列的注释
公开(公告)号：US20060210972A1
公开(公告)日：2006-09-21
申请号：US10507257
申请日：2003-03-13
申请人： Jonathan Arthur , Marc Wilkins , Mathew Traini
发明人： Jonathan Arthur , Marc Wilkins , Mathew Traini
IPC分类号： C12Q1/68 , G06F19/00
CPC分类号： C07K14/35 , C07K5/1008 , C07K14/47 , G16B20/00 , G16B30/00
摘要： A method of identifying one or more proteins in an unannotated DNA sequence is disclosed. The method involves dividing the DNA sequence into a plurality of sequence fragments of substantially the same length (about 300 to 5000 base pairs, most typically 1000 to 1050 base pairs. A six frame translation is then performed on each of the DNA sequence fragments to obtain six translated amino acid sequence fragments for each DNA sequence fragment. Each of the translated sequence fragments is subjected to theoretical digestion to obtain a plurality of cleaved peptide sequences. Next experimental empirical data for peptide fragments from a protein digested in the same manner as the theoretical digestion is compared with the theoretical data generated in step for each of the translated sequence fragments to identify one or more translated sequence fragments which include a substantial number of peptides present in the digested protein. The sequence fragment which has the greatest number of theoretical peptide masses correlating to the empirical data indicates the likely location of the protein of interest in the DNA sequence. To avoid problem where the sequence is divided at the site of a protein, the DNA sequence is duplicated and the original and duplicate are split in such a manner that the sequence fragments from the original overlap the cuts in the original genome sequence.
摘要翻译：公开了鉴定未记录DNA序列中的一种或多种蛋白质的方法。该方法包括将DNA序列划分成大致相同长度的多个序列片段（约300至5000个碱基对，最典型地为1000至1050个碱基对），然后对每个DNA序列片段进行六帧翻译以获得每个DNA序列片段的6个翻译的氨基酸序列片段，将每个翻译的序列片段进行理论消化以获得多个切割的肽序列。来自与理论相同方式消化的蛋白质的肽片段的下一个实验经验数据将消化与每个翻译的序列片段的步骤中产生的理论数据进行比较，以鉴定包含存在于消化蛋白质中的大量肽的一个或多个翻译的序列片段，具有最大数目的理论肽质量的序列片段与经验数据相关表明可能的位置 DNA序列中的目的蛋白质。为了避免序列在蛋白质位点分裂的问题，DNA序列被重复，原始和重复的分裂方式使原始序列的序列与原始基因组序列中的切割重叠。

2. 发明申请

US20050164404A1 Diagnostic testing process 有权
标题翻译：诊断测试过程
公开(公告)号：US20050164404A1
公开(公告)日：2005-07-28
申请号：US10497925
申请日：2002-12-12
申请人： David Marlborugh , Andrew Sloane , Robert Cole , William Hunter
发明人： David Marlborugh , Andrew Sloane , Robert Cole , William Hunter
IPC分类号： G01N33/52 , B01L3/00 , G01N33/48 , G01N33/543 , G01N33/545 , G01N33/553 , G01N33/558
CPC分类号： B01L3/5023 , B01L2200/0642 , B01L2300/0825 , B01L2300/16 , B01L2400/0406 , B01L2400/0633 , G01N33/54306 , G01N33/54366 , Y02A50/58 , Y10S435/81 , Y10S435/973 , Y10S436/807 , Y10S436/81
摘要： A method and apparatus for use in a flow through assay process is disclosed. The method is characterised by a “pre-incubation step” in which the sample which is to be analysed (typically for the presence of a particular protein), and a detection analyte (typically one or more antibodies bound to colloidal gold or a fluorescent tag) which is known to bind to the particular protein may bind together for a desired period of time. This pre-incubation step occurs before the mixture of sample and detection analyte come into contact with a capture analyte bound to a membrane. The provision of the pre-incubation step has the effect of both improving the sensitivity of the assay and reducing the volume of sample required for an assay. An apparatus for carrying out the method is disclosed defining a pre-incubation chamber for receiving the sample and detection analyte having a base defined by a membrane and a second membrane to which a capture analyte is bound. In one version the pre-incubation chamber is supported above the second membrane in one position but can be pushed into contact with the membrane carrying the capture analyte thus permitting fluid transfer from the incubation chamber through the capture membrane. In another version the membrane at the base of the incubation chamber is hydrophobic and its underside contacts the capture membrane and when a wetting agent is applied to the contents of the pre-incubation chamber fluid transfer occurs.
摘要翻译：公开了一种用于流过测定过程的方法和装置。该方法的特征在于“预孵育步骤”，其中待分析的样品（通常为特定蛋白质的存在）和检测分析物（通常为结合胶体金或荧光标记的一种或多种抗体）已知结合特定蛋白质可以结合在一起所需的时间段。此预孵育步骤在样品和检测分析物的混合物与结合到膜的捕获分析物接触之前发生。提供预孵育步骤具有提高测定的灵敏度并降低测定所需样品的体积的效果。公开了一种用于实施该方法的装置，其限定用于接收样品的预孵育室和具有由膜和第二膜限定的基底的检测分析物，捕获分析物与该第二膜结合。在一个版本中，预孵育室在一个位置上被支撑在第二膜上方，但是可以被推入与承载捕获分析物的膜接触，从而允许流体从孵育室通过捕获膜转移。在另一个版本中，孵育室底部的膜是疏水性的并且其下侧接触捕获膜，并且当将润湿剂施加到预孵育室的内容物时，发生流体转移。

3. 发明申请

US20050124077A1 Diagnostic testing process and apparatus 有权
标题翻译：诊断测试过程和设备
公开(公告)号：US20050124077A1
公开(公告)日：2005-06-09
申请号：US10487052
申请日：2002-08-20
申请人： Robert Cole , Andrew Sloane , William Hunter
发明人： Robert Cole , Andrew Sloane , William Hunter
IPC分类号： B01L3/00 , G01N33/543
CPC分类号： B01L3/5023 , B01L2200/0642 , B01L2300/0825 , B01L2300/16 , B01L2400/0406 , B01L2400/0633 , G01N33/54306 , G01N33/54366 , Y02A50/58 , Y10S435/81 , Y10S435/973 , Y10S436/81
摘要： A method and apparatus for use in a flow through assay process is disclosed. The method is characterised by a “pre-incubation step” in which the sample which is to be analysed, (typically for the presence of a particular protein), and a detection analyte (typically an antibody bound to colloidal gold or a fluorescent tag) which is known to bind to the particular protein may bind together for a desired period of time. This pre incubation step occurs before the mixture of sample and detection analyte come into contact with a capture analyte bound to a membrane. The provision of the pre-incubation step has the effect of both improving the sensitivity of the assay and reducing the volume of sample required for an assay. An apparatus for carrying out the method is disclosed defining a pre-incubation chamber for receiving the sample and detection analyte having a base defined by a membrane and a second membrane to which a capture analyte is bound. In one version the pre-incubation chamber is supported above the second membrane in one position but can be pushed into contact with the membrane carrying the capture analyte thus permitting fluid transfer from the incubation chamber through the capture membrane. In another version the membrane at the base of the incubation chamber is hydrophobic and its underside contacts the capture membrane and when a wetting agent is applied to the contents of the pre-incubation chamber fluid transfer occurs.
摘要翻译：公开了一种用于流过测定过程的方法和装置。该方法的特征在于“预孵育步骤”，其中待分析的样品（通常为特定蛋白质的存在）和检测分析物（通常为与胶体金或荧光标签结合的抗体）已知结合特定蛋白质的蛋白质可以结合在一起所需的时间段。该预孵育步骤在样品和检测分析物的混合物与结合到膜的捕获分析物接触之前发生。提供预孵育步骤具有提高测定的灵敏度并降低测定所需样品的体积的效果。公开了一种用于实施该方法的装置，其限定用于接收样品的预孵育室和具有由膜和第二膜限定的基底的检测分析物，捕获分析物与该第二膜结合。在一个版本中，预孵育室在一个位置上被支撑在第二膜上方，但是可以被推入与承载捕获分析物的膜接触，从而允许流体从孵育室通过捕获膜转移。在另一个版本中，孵育室底部的膜是疏水性的并且其下侧接触捕获膜，并且当将润湿剂施加到预孵育室的内容物时，发生流体转移。

4. 发明申请

US20090208535A1 Novel Methods of Diagnosis of Treatment of P. Aeruginosa Infection and Reagents Therefor 审中-公开
标题翻译：新型铜绿假单胞菌感染及其试剂治疗方法
公开(公告)号：US20090208535A1
公开(公告)日：2009-08-20
申请号：US11571036
申请日：2005-06-28
申请人： Andrew John Sloane , Susanne Kartin Pedersen , Ron Weinberger
发明人： Andrew John Sloane , Susanne Kartin Pedersen , Ron Weinberger
IPC分类号： A61K39/02 , G01N33/53 , A61P37/04
CPC分类号： C07K16/1214 , G01N33/56911 , G01N2333/21
摘要： The present invention relates to novel diagnostic, prognostic and therapeutic reagents for infection of an animal subject such as a human by Pseudomonas aeruginosa, and conditions associated with such infections, such as, for example, an acute clinical exacerbation in a cystic fibrosis (CF) subject. In particular, the present invention relates to methods for diagnosing/prognosing an infection by P. aeruginosa in a subject comprising detecting the presence or amount of one or more proteins of P. aeruginosa or a fragment or epitope thereof or an antibody thereto in a sample from the subject.
摘要翻译：本发明涉及用于通过绿脓假单胞菌感染动物受试者例如人的新型诊断，预后和治疗试剂，以及与这种感染相关的病症，例如囊性纤维化（CF）中的急性临床恶化，学科。特别地，本发明涉及用于诊断/预测受试者中铜绿假单胞菌感染的方法，包括检测样品中铜绿假单胞菌的一种或多种蛋白质或其片段或表位或抗体的存在或量从主题。

5. 发明申请

US20070178541A1 Method of isolating a protein 审中-公开
标题翻译：分离蛋白质的方法
公开(公告)号：US20070178541A1
公开(公告)日：2007-08-02
申请号：US10562132
申请日：2004-06-28
申请人： Susanne Pedersen , Robert Cole , Ron Weinberger , Andrew Sloane
发明人： Susanne Pedersen , Robert Cole , Ron Weinberger , Andrew Sloane
IPC分类号： C12Q1/70 , G01N33/569 , G01N33/554 , G01N33/543
CPC分类号： G01N33/6854 , G01N33/564 , G01N33/569
摘要： The present invention provides a method for isolating and/or identifying an immunogenic protein from a protein complex comprising an immunoglobulin or a mixture thereof or an immunoglobulin-containing fraction.
摘要翻译：本发明提供了用于从包含免疫球蛋白或其混合物或含免疫球蛋白的级分的蛋白质复合物中分离和/或鉴定免疫原性蛋白的方法。

6. 发明授权

US07205159B2 Diagnostic testing process and apparatus 有权
公开(公告)号：US07205159B2
公开(公告)日：2007-04-17
申请号：US10487052
申请日：2002-08-20
申请人： Robert Alan Cole , Andrew John Sloane , William Samuel Hunter
发明人： Robert Alan Cole , Andrew John Sloane , William Samuel Hunter
IPC分类号： G01N33/543
CPC分类号： B01L3/5023 , B01L2200/0642 , B01L2300/0825 , B01L2300/16 , B01L2400/0406 , B01L2400/0633 , G01N33/54306 , G01N33/54366 , Y02A50/58 , Y10S435/81 , Y10S435/973 , Y10S436/81
摘要： A method and apparatus for use in a flow through assay process is disclosed. The method is characterised by a “pre-incubation step” in which the sample which is to be analysed, (typically for the presence of a particular protein), and a detection analyte (typically an antibody bound to colloidal gold or a fluorescent tag) which is known to bind to the particular protein may bind together for a desired period of time. This pre incubation step occurs before the mixture of sample and detection analyte come into contact with a capture analyte bound to a membrane. The provision of the pre-incubation step has the effect of both improving the sensitivity of the assay and reducing the volume of sample required for an assay. An apparatus for carrying out the method is disclosed defining a pre-incubation chamber for receiving the sample and detection analyte having a base defined by a membrane and a second membrane to which a capture analyte is bound. In one version the pre-incubation chamber is supported above the second membrane in one position but can be pushed into contact with the membrane carrying the capture analyte thus permitting fluid transfer from the incubation chamber through the capture membrane. In another version the membrane at the base of the incubation chamber is hydrophobic and its underside contacts the capture membrane and when a wetting agent is applied to the contents of the pre-incubation chamber fluid transfer occurs.

7. 发明申请

US20100323369A1 Diagnostic Testing Process 有权
标题翻译：诊断测试过程
公开(公告)号：US20100323369A1
公开(公告)日：2010-12-23
申请号：US12874675
申请日：2010-09-02
申请人： David Ian Marlborough , Andrew John Sloane , Robert Alan Cole , William Samuel Hunter
发明人： David Ian Marlborough , Andrew John Sloane , Robert Alan Cole , William Samuel Hunter
IPC分类号： G01N33/53 , G01N30/00 , C12M1/34 , G01N33/543
CPC分类号： B01L3/5023 , B01L2200/0642 , B01L2300/0825 , B01L2300/16 , B01L2400/0406 , B01L2400/0633 , G01N33/54306 , G01N33/54366 , Y02A50/58 , Y10S435/81 , Y10S435/973 , Y10S436/807 , Y10S436/81
摘要： A method and apparatus for use in a flow through assay process is disclosed. The method is characterised by a “pre-incubation step” in which the sample which is to be analysed (typically for the presence of a particular protein), and a detection analyte (typically one or more antibodies bound to colloidal gold or a fluorescent tag) which is known to bind to the particular protein may bind together for a desired period of time. This pre-incubation step occurs before the mixture of sample and detection analyte come into contact with a capture analyte bound to a membrane. The provision of the pre-incubation step has the effect of both improving the sensitivity of the assay and reducing the volume of sample required for an assay. An apparatus for carrying out the method is disclosed defining a pre-incubation chamber for receiving the sample and detection analyte having a base defined by a membrane and a second membrane to which a capture analyte is bound. In one version the pre-incubation chamber is supported above the second membrane in one position but can be pushed into contact with the membrane carrying the capture analyte thus o permitting fluid transfer from the incubation chamber through the capture membrane. In another version the membrane at the base of the incubation chamber is hydrophobic and its underside contacts the capture membrane and when a wetting agent is applied to the contents of the pre-incubation chamber fluid transfer occurs.
摘要翻译：公开了一种用于流过测定过程的方法和装置。该方法的特征在于“预孵育步骤”，其中待分析的样品（通常为特定蛋白质的存在）和检测分析物（通常为结合胶体金或荧光标记的一种或多种抗体）已知结合特定蛋白质可以结合在一起所需的时间段。此预孵育步骤在样品和检测分析物的混合物与结合到膜的捕获分析物接触之前发生。提供预孵育步骤具有提高测定的灵敏度并降低测定所需样品的体积的效果。公开了一种用于实施该方法的装置，其限定用于接收样品的预孵育室和具有由膜和第二膜限定的基底的检测分析物，捕获分析物与该第二膜结合。在一个版本中，预孵育室在一个位置上被支撑在第二膜上方，但是可以被推入与携带捕获分析物的膜接触，从而允许流体通过捕获膜从孵育室转移。在另一个版本中，孵育室底部的膜是疏水性的并且其下侧接触捕获膜，并且当将润湿剂施加到预孵育室的内容物时，发生流体转移。

8. 发明申请

US20080142695A1 Characterisation of Glycans 审中-公开
标题翻译：糖的表征
公开(公告)号：US20080142695A1
公开(公告)日：2008-06-19
申请号：US10560193
申请日：2004-06-10
申请人： Hiren Joshi , Coran Niclas Karlsson , Benjamin Schulz
发明人： Hiren Joshi , Coran Niclas Karlsson , Benjamin Schulz
IPC分类号： B01D59/44
CPC分类号： G16C20/20 , H01J49/0027
摘要： A method for characterising the structure or sub-structure of a glycan or glycan derivative, experimentally derives the mass of an unidentified glycan molecule and compares the mass of the unidentified glycan molecule with identified and characterised glycan structures to select candidate structures for the glycan molecule. Next the mass of fragments of the glycan molecule is experimentally derived and theoretical fragmentation of the selected candidates is conducted. The mass of the fragments of the unidentified glycan molecule is matched with the mass of fragments theoretically derived from the candidate structures. Next scoring occurs to produce ranked confidence scores for each of the candidate structures by comparing the masses of the experimentally derived fragments with the masses of the theoretically derived fragments. Two scoring methods are disclosed namely segmentation and correspondence scoring. If insufficient confidence is obtained in the highest ranked score, further including the step of repeating the process by taking into account more complex cleavage patterns in the theoretical fragmentation step, or by obtaining further spectra.
摘要翻译：用于表征聚糖或聚糖衍生物的结构或亚结构的方法实验地得到不明的聚糖分子的质量，并将未鉴别的聚糖分子的质量与鉴定的和表征的聚糖结构进行比较，以选择聚糖分子的候选结构。接下来，聚糖分子的片段的质量是实验导出的，并且进行所选候选物的理论碎片化。未鉴定的聚糖分子的片段的质量与从候选结构理论上衍生的片段的质量匹配。通过将实验得到的片段的质量与理论上衍生的片段的质量进行比较，进行下一个评分，以产生每个候选结构的置信度得分。公开了两个评分方法，即分割和通信评分。如果在最高排名得分中没有获得足够的置信度，还包括通过考虑理论碎片化步骤中更复杂的切割模式或通过获得进一步的光谱来重复该过程的步骤。

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