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81. 发明授权

US5750411A Sol particle decay protection immunoassay 失效
标题翻译：溶胶颗粒衰减保护免疫测定
公开(公告)号：US5750411A
公开(公告)日：1998-05-12
申请号：US656904
申请日：1996-06-03
申请人： Ronald G. Sommer
发明人： Ronald G. Sommer
IPC分类号： G01N33/53 , G01N33/543 , G01N33/553 , G01N33/58 , G01N33/544
CPC分类号： G01N33/553 , G01N33/585
摘要： Disclosed is a method for determining the concentration of an analyte in a liquid test sample. The method involves the use of colloidal sized metal particles, which exhibit a spectral response in the agglomerated state which is detectably different than in their unagglomerated state, which bear specific binding partners for the analyte in question on their surface. The specific binding partner treated colloidal particles are combined in a buffered, aqueous medium with a destabilizer material capable of destabilizing the specific binding partner-colloidal particle conjugates thereby permitting the particles to agglomerate. Also included in the liquid medium is a polymer bearing multiple analyte or analyte analog molecules along its surface which serves to protect the specific binding partner coated metal particles from dissociation by the destabilizing agent. Analyte added to the medium, typically in the form of a fluid test sample, competes for the specific binding partner and thereby decreases the amount of protection afforded by the analyte or analyte analog bearing polymer which permits unprotected colloidal particles to agglomerate and provide a detectable spectral response. The magnitude of the change in spectral response is directly proportional to the concentration of analyte in the fluid test sample.
摘要翻译：公开了一种用于测定液体测试样品中分析物浓度的方法。该方法包括使用胶体大小的金属颗粒，其在附聚状态下显示出可见的不同于其未凝集状态的光谱响应，其在其表面上承载所讨论的分析物的特异性结合配偶体。将特异性结合配偶体处理的胶体颗粒在缓冲的含水介质中与能够使特异性结合配偶体 - 胶体粒子共轭物不稳定的去稳定剂材料组合，从而允许颗粒附聚。还包括在液体介质中的是具有沿其表面的多个分析物或分析物类似物分子的聚合物，其用于保护特异性结合配偶体涂覆的金属颗粒免受不稳定剂的分解。通常以流体测试样品的形式添加到培养基中的分析物竞争特异性结合配偶体，从而减少被分析物或分析物模拟轴承聚合物提供的保护的量，其允许未受保护的胶体颗粒附聚并提供可检测的光谱响应。光谱响应变化的大小与流体测试样品中分析物的浓度成正比。

82. 发明授权

US5738986A Analytical reagent particle with covalently-bound enzyme 失效
标题翻译：具有共价结合酶的分析试剂颗粒
公开(公告)号：US5738986A
公开(公告)日：1998-04-14
申请号：US730966
申请日：1996-10-16
申请人： Kurt G. I. Nilsson
发明人： Kurt G. I. Nilsson
IPC分类号： G01N33/543 , G01N33/58 , G01N33/53
CPC分类号： G01N33/54313 , G01N33/581 , G01N33/585 , Y10S436/829
摘要： The invention relates to a reagent consisting of at least one enzyme and at least one other substance, which are covalently or noncovalently bound to a particle, which is smaller than or is equal to 1000 Angstrom in diameter. The invention also relates to method and use of the reagent for determination or studies of a cell or a virus or another component in a sample. According to the invention, these determinations are made with for example some ELISA-technique (competitive, sandwich, etc.), employing the reagent preferrably in the form of a suspension in buffered water, instead of and in the same way as described for soluble enzyme conjugates. The substance can be an antibody, a lectin, avidin or an antigen, the enzyme can be for example peroxidase, alkaline phosphatase, and the particle can be an inorganic or an organic polymeric compound or a combination thereof, as, for example, tresyl-activated glycerylpropyl-silica.
摘要翻译：本发明涉及由至少一种酶和至少一种其它物质组成的试剂，其与颗粒共价或非共价结合，其直径小于或等于1000埃。本发明还涉及用于测定或研究样品中的细胞或病毒或其它成分的试剂的方法和用途。根据本发明，这些测定是用例如一些ELISA技术（竞争性，夹心等）进行的，使用试剂优选以缓冲水中的悬浮液的形式，而不是以与可溶性的相同的方式酶缀合物。该物质可以是抗体，凝集素，抗生物素蛋白或抗原，酶可以是例如过氧化物酶，碱性磷酸酶，并且该颗粒可以是无机或有机聚合物或其组合，例如，活化甘油基丙基二氧化硅。

83. 发明授权

US5716855A Fluorescent latex containing at least two fluorochromes, process for producing it and application thereof 失效
标题翻译：含有至少两种荧光染料的荧光胶乳，其制备方法及其应用
公开(公告)号：US5716855A
公开(公告)日：1998-02-10
申请号：US586673
申请日：1996-03-01
申请人： Dan Lerner , Frederic Ricchiero , Joel Richard , Dominique Teychenne , Sophie Vaslin
发明人： Dan Lerner , Frederic Ricchiero , Joel Richard , Dominique Teychenne , Sophie Vaslin
IPC分类号： G01N33/533 , C08F2/22 , C08F2/44 , C08K5/00 , C08K9/10 , C08L101/00 , C09B67/08 , C09K11/06 , G01N33/545 , G01N33/58 , G01N21/64 , C09K11/02
CPC分类号： G01N33/585 , C08K5/0041 , C08K9/10 , G01N33/582 , G01N2800/52 , Y10S436/80
摘要： A fluorescent latex with particles containing at least one encapsulated hydrophobic fluorochrome A (acceptor) and at least one encapsulated hydrophobic fluorochrome D (donor), wherein A is different from D and exposure to light radiation enables the emission from fluorochrome D to excite fluorochrome A, and a method for producing said latex and its use as a marker, particularly a biological marker, are also disclosed.
摘要翻译： PCT No.PCT / FR94 / 00867 Sec。 371日期1996年3月1日 102（e）1996年3月1日PCT 1994年11月7日PCT PCT。出版物WO95 / 02635 日期1995年1月26日具有包含至少一个包封的疏水性荧光染料A（受体）和至少一个封装的疏水性荧光染料D（供体）的颗粒的荧光胶乳，其中A与D不同并且暴露于光辐射使得能够从荧光染料D 激发荧光染料A，并且还公开了制备所述胶乳的方法及其作为标记物，特别是生物标记物的用途。

84. 发明授权

US5681755A Method for depositing metal particles on a marker 失效
公开(公告)号：US5681755A
公开(公告)日：1997-10-28
申请号：US467656
申请日：1995-06-06
申请人： Marcus J. M. Noppe , Frank J. Konings
发明人： Marcus J. M. Noppe , Frank J. Konings
IPC分类号： G01N33/532 , G01N33/553 , G01N33/58 , G03C5/58 , G01N33/533 , G03C5/30
CPC分类号： G03C5/58 , G01N33/532 , G01N33/553 , G01N33/585
摘要： An improved method for depositing metal particles on a marker which catalyzes the reduction of metal ions from a physical developer comprising a solution of metal ions, a molar excess of complexant in respect to the metal ions and a reducing agent. Said method being preferably employed for the detection of one or more components of an aggregate formed between a least one specific binding agent and its corresponding bindable substance by labelling at least one component of said aggregate with a marker and contacting said aggregate with a physical developer, whereby under influence of the marker a metal particle is formed which can be detected. Further the invention also relates to solutions and test-kits adapted for carrying out the above mentioned method.

85. 发明授权

US5674755A Method for depositing metal particles on a marker 失效
标题翻译：将金属颗粒沉积在标记上的方法
公开(公告)号：US5674755A
公开(公告)日：1997-10-07
申请号：US467655
申请日：1995-06-06
申请人： Marcus J. M. Noppe , Frank J. Konings
发明人： Marcus J. M. Noppe , Frank J. Konings
IPC分类号： G01N33/532 , G01N33/553 , G01N33/58 , G03C5/58 , G01N33/533 , G03C5/30
CPC分类号： G03C5/58 , G01N33/532 , G01N33/553 , G01N33/585
摘要： An improved method for depositing metal particles on a marker which catalyzes the reduction of metal ions from a physical developer comprising a solution of metal ions, a molar excess of complexant in respect to the metal ions and a reducing agent. Said method being preferably employed for the detection of one or more components of an aggregate formed between a least one specific binding agent and its corresponding bindable substance by labelling at least one component of said aggregate with a marker and contacting said aggregate with a physical developer, whereby under influence of the marker a metal particle is formed which can be detected. Further the invention also relates to solutions and test-kits adapted for carrying out the above mentioned method.
摘要翻译：一种用于在标记上沉积金属颗粒的改进方法，该方法催化从物理显影剂中还原金属离子，所述物理显影剂包括金属离子溶液，相对于金属离子摩尔过量的络合物和还原剂。所述方法优选用于通过用标记物标记所述骨料的至少一种组分并将所述聚集体与物理显影剂接触来检测形成在至少一种特异性结合剂和其相应的可结合物质之间的聚集体的一种或多种组分，由此在标记的影响下形成可以检测到的金属颗粒。此外，本发明还涉及适于实施上述方法的溶液和试剂盒。

86. 发明授权

US5641689A Stable aqueous carbon sol composition for determining analyte 失效
标题翻译：用于测定分析物的稳定的水性碳溶胶组合物
公开(公告)号：US5641689A
公开(公告)日：1997-06-24
申请号：US546366
申请日：1995-10-20
申请人： Albert Willem Jacob Van Doorn , Jan Herman Wichers , Wilhelmus Martinus Jozef Van Gelder
发明人： Albert Willem Jacob Van Doorn , Jan Herman Wichers , Wilhelmus Martinus Jozef Van Gelder
IPC分类号： B01J13/00 , C07H23/00 , G01N33/543 , G01N33/58 , G01N33/551
CPC分类号： G01N33/54346 , B01J13/0004 , B01J13/0026 , C07H23/00 , G01N33/54313 , G01N33/581 , G01N33/585 , Y10S435/962 , Y10S435/97 , Y10S436/81 , Y10S516/922
摘要： This invention relates to a composition for use in a method for determining the presence or amount of an analyte in a sample comprising a labeled constituent consisting of an aqueous carbon sol which is stable in the absence of a stabilizing substance. The colloidal carbon particles have directly conjugated to their surface a binding component which specifically binds to the analyte and the resulting analyte/carbon particle complex is used as an indication of the presence or amount of analyte in the sample. The grade of carbon used satisfies the condition V>0 wherein V is a linear predictor value according to the formula V=-138.954-0.987.times.DBP+15,609.times.VC+3.994.times.PPD, wherein DBP is the dibutylphthalate adsorption in ml/100 g, as determined according to DIN 53601; VC is the volatile content in %, as determined according to DIN 53552; and PPD is the average primary particle diameter in nanometers.
摘要翻译：本发明涉及一种用于确定样品中分析物的存在或量的方法的组合物，其包含由不存在稳定物质时稳定的水性碳溶胶组成的标记成分。胶体碳颗粒与其表面直接缀合具有特异性结合分析物的结合成分，并将所得分析物/碳颗粒复合物用作样品中分析物的存在或数量的指示。使用的碳满足条件V> 0，其中V是根据公式V = -138.954-0.987xDBP + 15,609xVC + 3.994xPPD的线性预测值，其中DBP是以毫升/ 100g测定的邻苯二甲酸二丁酯吸附量根据DIN 53601; VC是根据DIN 53552确定的挥发物含量％。 PPD是以纳米为单位的平均一次粒径。

87. 发明授权

US5635362A Assay of blood or other biologic samples for target analytes 失效
标题翻译：测定目标分析物的血液或其他生物样品
公开(公告)号：US5635362A
公开(公告)日：1997-06-03
申请号：US247336
申请日：1994-05-23
申请人： Robert A. Levine , Stephen C. Wardlaw , Rodolfo R. Rodriguez , Adrien P. Malick , Alvydas J. Ozinskas
发明人： Robert A. Levine , Stephen C. Wardlaw , Rodolfo R. Rodriguez , Adrien P. Malick , Alvydas J. Ozinskas
IPC分类号： G01N33/543 , B01L3/14 , G01N33/49 , G01N33/537 , G01N33/569 , G01N33/58 , G01N33/558
CPC分类号： B01L3/5021 , G01N33/491 , G01N33/5375 , G01N33/56972 , G01N33/585 , Y10S435/81 , Y10S435/967 , Y10S435/971 , Y10S435/973 , Y10S436/805 , Y10S436/81 , Y10S436/824 , Y10S436/829 , Y10T436/111666
摘要： A patient's health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. Each density-defined body carries analyte-capture binding materials such as antigens or antibodies, which are specific to an epitope, or other specific high affinity binding site on a target analyte which target analyte may be in the blood or other sample being tested; and the level of which analyte is indicative of the patient's health. At least one labeled binding material which is also specific to an epitope, or other specific high affinity binding site on the target analyte is added to the sample so as to form labeled binding material/analyte/body complexes in the sample. Upon centrifugation, the complexes will settle out in different areas in the tube according to the respective density of the body or bodies; and the degree of label emission of the complex layers can enable qualitative and/or quantitative analyses of the sample to be made. Unbound labeled binding materials will be separated from the complexed layers by the washing action of ascending or descending components of the sample during the centrifugation step. Unbound labeled binding material will thus not interfere with the analysis.
摘要翻译：可以通过将透明管中的血液样品离心来诊断患者的健康，该管包含一个或多个不同密度的浮体，插入物，脂质体或塑料珠的主体或组。每个密度定义的身体携带分析物 - 捕获结合材料，例如抗原或抗体，其对靶分析物是特异性的，或靶分析物上的其它特异性高亲和力结合位点，其目标分析物可能在待测试的血液或其他样品中; 并且其分析物的水平表示患者的健康。将至少一种对靶分析物上的表位或其他特异性高亲和力结合位点特异性的标记结合物质加入到样品中，以便在样品中形成标记的结合材料/分析物/身体复合物。离心后，复合物将根据身体或身体的相应密度沉淀在管中的不同区域; 并且复合层的标签发射程度可以使得要进行样品的定性和/或定量分析。通过在离心步骤期间样品的上升或下降组分的洗涤作用，未结合的标记结合材料将从复合层分离。因此，未结合的标签结合材料不会影响分析。

88. 发明授权

US5583056A Method for preparing aqueous suspension for diagnostic tests 失效
标题翻译：制备用于诊断试验的水性悬浮液的方法
公开(公告)号：US5583056A
公开(公告)日：1996-12-10
申请号：US439624
申请日：1995-05-12
申请人： Wilfridus M. Brouwer
发明人： Wilfridus M. Brouwer
IPC分类号： A61B10/00 , A61K47/48 , G01N33/543 , G01N33/545 , G01N33/58 , G01N33/551 , G01N33/553
CPC分类号： G01N33/585 , G01N33/54313 , Y10S435/975 , Y10T428/2982 , Y10T428/2984 , Y10T428/2989 , Y10T428/2991 , Y10T428/2996 , Y10T428/2998
摘要： The invention relates to an aqueous suspension for diagnostic or immunodiagnostic tests, comprising non-polymer nuclei surrounded by a hydrophilic copolymer that contains functional groups, and also to a method for the preparation of this suspension. The method involves adding a monomer mixture to a colloidal dispersion of non-polymer particles, the monomer mixture being so chosen that the resultant copolymer has a charge of identical sign to that of the original dispersion. The invention also relates to a method for the detection of a specifically binding substance or immunochemically active component in a test fluid, and to a reagent and a test kit to be used when employing said detection methods.
摘要翻译：本发明涉及用于诊断或免疫诊断试验的水性悬浮液，其包含由含有官能团的亲水共聚物包围的非聚合物核，以及制备该悬浮液的方法。该方法包括将单体混合物加入到非聚合物颗粒的胶体分散体中，选择单体混合物，使得所得共聚物具有与原始分散体相同的电荷。本发明还涉及一种用于检测测试流体中的特异性结合物质或免疫化学活性成分的方法，以及在使用所述检测方法时使用的试剂和试剂盒。

89. 发明授权

US5578577A Method for storing labile proteins 失效
标题翻译：储存不稳定蛋白的方法
公开(公告)号：US5578577A
公开(公告)日：1996-11-26
申请号：US469714
申请日：1995-06-06
申请人： Shanfun Ching , Patricia A. Billings , Julian Gordon
发明人： Shanfun Ching , Patricia A. Billings , Julian Gordon
IPC分类号： G01N30/88 , G01N30/90 , G01N33/532 , G01N33/543 , G01N33/558 , G01N33/58 , G01N33/76 , G01N37/00 , A61K38/00 , C07K1/00
CPC分类号： G01N33/587 , G01N33/54366 , G01N33/54386 , G01N33/54393 , G01N33/558 , G01N33/585 , G01N33/76 , G01N30/90 , Y10S435/967 , Y10S435/97 , Y10S435/975 , Y10S436/801 , Y10S436/807 , Y10S436/808 , Y10S436/826 , Y10S514/97
摘要： The present invention relates to improved specific binding assay methods, kits and devices utilizing chromatographically mobile specific binding reagents labelled with colloidal particles. Specific binding reagents labelled with colloidal particles such as gold and selenium may be subjected to rapid chromatographic solvent transport on chromatographic media by means of selected solvents and chromatographic transport facilitating agents. Further, impregnation of solid substrate materials with labile protein materials including colloidal particle and enzyme labelled reagents in the presence of meta-soluble proteins provides for the rapid resolubilization of such materials which have been dried onto such substrate materials.
摘要翻译：本发明涉及改进的特异性结合测定方法，使用用胶体颗粒标记的色谱移动的特异性结合试剂的试剂盒和装置。用胶体颗粒如金和硒标记的特异性结合剂可以通过选择的溶剂和色谱传输促进剂在色谱介质上进行快速色谱溶剂转移。此外，在存在可溶性蛋白质的情况下，用包括胶体颗粒和酶标记的试剂的不稳定蛋白质材料浸渍固体底物材料，使得已经干燥到这种基材上的这些材料的快速再溶解。

90. 发明授权

US5326692B1 Fluorescent microparticles with controllable enhanced stokes shift 失效
标题翻译：荧光微粒具有可控增强的斯托克斯位移
公开(公告)号：US5326692B1
公开(公告)日：1996-04-30
申请号：US88229992
申请日：1992-05-13
申请人： MOLECULAR PROBES INC
发明人： BRINKLEY JOHN M , HAUGLAND RICHARD P , SINGER VICTORIA L
IPC分类号： G01N33/53 , C09B57/00 , C09B67/22 , C09K11/06 , C09K11/07 , C12N15/09 , C12Q1/68 , G01N21/78 , G01N33/533 , G01N33/543 , G01N33/545 , G01N33/58 , C09K11/02
CPC分类号： C12Q1/6818 , C09K11/06 , G01N33/533 , G01N33/585 , Y10S436/80 , Y10S436/805 , Y10T428/2982

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