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    • 85. 发明申请
    • Cartridge for nucleic acid separation and purification and method for producing the same
    • 用于核酸分离和纯化的芯片及其制备方法
    • US20070148649A1
    • 2007-06-28
    • US10576756
    • 2004-09-21
    • Keiji ShigesadaMorio FujiwaraToshihiro Mori
    • Keiji ShigesadaMorio FujiwaraToshihiro Mori
    • C12Q1/68C12M1/34
    • B01D63/081B01D63/08B01D2311/2626B01D2313/04B01L3/50255B01L2200/0631B01L2200/12B01L2300/0829B29C45/14311B29C45/14377B29C45/14467B29C2045/14319B29L2031/14
    • A cartridge for nucleic acid separation and purification and a method for producing the cartridge are provided which does not require a special facility for adhering containers and can produce a number of cartridges at the same time. Also is provided a cartridge for nucleic acid separation and purification in which a washing liquid is prevented from remaining in the container during washing step of nucleic acid separation and purification, and liquids is prevented from attaching to an outer wall surface of a discharge part. Specifically, a cartridge for nucleic acid separation and purification (100) is composed of: an insert member (110) including a bottom member (120) and a nucleic acid-adsorptive porous membrane (F); and a barrel (140) which is formed by insert injection molding with the insert member (110). A rib (226) of the cartridge for nucleic acid separation and purification (200) slopes down to the discharge part (224) in a direction from an outer side end part (226a) to an inner side end part (226b). A thickness (T) at an end face (324), which is a part forming the second opening (321) of the cap (320) of the cartridge for nucleic acid separation and purification (300), is made 0.2 mm or more.
    • 提供了用于核酸分离和纯化的滤芯以及用于生产墨盒的方法,其不需要用于粘附容器的特殊设备并且可以同时生产多个墨盒。 还提供了一种用于核酸分离和纯化的盒,其中在核酸分离和纯化的洗涤步骤期间防止洗涤液保留在容器中,并且防止液体附着到排出部分的外壁表面。 具体地,用于核酸分离和纯化的盒(100)由以下部件组成:包括底部部件(120)和核酸吸附多孔膜(F)的插入部件(110) 以及通过与插入构件(110)的插入注射成型形成的筒(140)。 用于核酸分离和纯化的药筒(200)的肋(226)在从外侧端部(226a)到内侧端部(226b)的方向上向下倾斜到排出部(224)。 作为形成核酸分离纯化用滤芯(300)的盖(320)的第二开口(321)的部分的端面(324)的厚度(T)为0.2mm以上。