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81. 发明授权

US07943348B2 Method and apparatus for amplifying nucleic acids 有权
标题翻译：扩增核酸的方法和装置
公开(公告)号：US07943348B2
公开(公告)日：2011-05-17
申请号：US12504271
申请日：2009-07-16
申请人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
发明人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： G01N35/08 , B01F5/0646 , B01F5/0647 , B01F5/0654 , B01F13/0071 , B01F2015/0221 , B01J2219/00783 , B01J2219/00824 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/0086 , B01J2219/00873 , B01J2219/00891 , B01L3/5027 , B01L3/502715 , B01L2300/0809 , B01L2300/0861 , B01L2300/0883 , G01N2035/00366 , G01N2035/00514
摘要： A method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.
摘要翻译：用于扩增核酸的方法和装置。该方法包括通过不同的入口通道将含有用于核酸扩增的反应物的反应物水溶液和与反应物水溶液相分离并且不参与扩增反应的流体引入反应容器，形成多个反应物水溶液通过使反应物水溶液与反应容器中的流体接触，并且扩增反应物水溶液液滴中的核酸，由流体包围的液滴。该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器，以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

82. 发明授权

US07915013B2 Method and apparatus for amplifying nucleic acids 有权
标题翻译：扩增核酸的方法和装置
公开(公告)号：US07915013B2
公开(公告)日：2011-03-29
申请号：US12504213
申请日：2009-07-16
申请人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
发明人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： G01N35/08 , B01F5/0646 , B01F5/0647 , B01F5/0654 , B01F13/0071 , B01F2015/0221 , B01J2219/00783 , B01J2219/00824 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/0086 , B01J2219/00873 , B01J2219/00891 , B01L3/5027 , B01L3/502715 , B01L2300/0809 , B01L2300/0861 , B01L2300/0883 , G01N2035/00366 , G01N2035/00514
摘要： An apparatus for amplifying nucleic acids. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.
摘要翻译：扩增核酸的装置。该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器，以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

83. 发明授权

US07722751B2 Apparatus for regulating salt concentration using electrodialysis, lab-on-a-chip including the same, and method of regulating salt concentration using the apparatus 有权
标题翻译：使用电渗析法调节盐浓度的装置，包括该盐浓度的实验室的方法以及使用该装置调节盐浓度的方法
公开(公告)号：US07722751B2
公开(公告)日：2010-05-25
申请号：US11440872
申请日：2006-05-25
申请人： Jung-im Han , Young-sun Lee , Joon-ho Kim
发明人： Jung-im Han , Young-sun Lee , Joon-ho Kim
IPC分类号： B01D61/48
CPC分类号： B01D61/422 , B01D61/46 , B01L3/5027 , G01N2001/4011
摘要： An apparatus for regulating salt concentration, a lab-on-a-chip including the same and a method of regulating salt concentration using the apparatus are provided. The apparatus includes: a reaction chamber that is defined by a cation exchange membrane and an anion exchange membrane and is selected from the group consisting of a biomolecule extraction chamber, an amplification chamber, a hybridization chamber, and a detection chamber; a first electrode chamber that is defined by the anion exchange membrane and a first electrode and includes an ion exchange medium; and a second electrode chamber that is defined by the cation exchange membrane and a second electrode and includes an ion exchange medium. Even without injecting solutions with different salt concentrations into the reaction chamber by operating pumps and valves for each operation stage, the salt concentration can be reversibly regulated in situ by adjusting the polarity, intensity and application time of a voltage.
摘要翻译：提供了一种调节盐浓度的装置，包括该盐浓度的实验室，以及使用该装置调节盐浓度的方法。该装置包括：由阳离子交换膜和阴离子交换膜限定的反应室，其选自生物分子提取室，扩增室，杂交室和检测室; 由阴离子交换膜和第一电极限定并包括离子交换介质的第一电极室; 以及由阳离子交换膜和第二电极限定并包括离子交换介质的第二电极室。即使在每个操作阶段通过操作泵和阀都不会将具有不同盐浓度的溶液注入反应室中，可以通过调节电压的极性，强度和施加时间来原位调节盐浓度。

84. 发明申请

US20100125134A1 METHOD OF SEPARATING GENOMIC DNA AND PLASMID DNA FROM EACH OTHER AND KIT THEREFOR 审中-公开
标题翻译：从每个其他和套件中分离基因DNA和DNA片段的方法
公开(公告)号：US20100125134A1
公开(公告)日：2010-05-20
申请号：US12611201
申请日：2009-11-03
申请人： Hee-kyun LIM , Kyu-youn HWANG , Joon-ho Kim
发明人： Hee-kyun LIM , Kyu-youn HWANG , Joon-ho Kim
IPC分类号： C07H21/04
CPC分类号： C12N15/1006
摘要： Provided are a method of separating genome DNA and plasmid DNA from each other from a sample using a binding buffer containing a high concentration kosmotropic salt and chaotropic salt, and a kit therefor.
摘要翻译：本发明提供使用含有高浓度脱水盐和离液盐的结合缓冲液从试样中分离基因组DNA和质粒DNA的方法及其试剂盒。

85. 发明申请

US20100021972A1 METHOD AND APPARATUS FOR AMPLIFYING NUCLEIC ACIDS 有权
标题翻译：用于放大核酸的方法和装置
公开(公告)号：US20100021972A1
公开(公告)日：2010-01-28
申请号：US12504271
申请日：2009-07-16
申请人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
发明人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
IPC分类号： C12P19/34
CPC分类号： G01N35/08 , B01F5/0646 , B01F5/0647 , B01F5/0654 , B01F13/0071 , B01F2015/0221 , B01J2219/00783 , B01J2219/00824 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/0086 , B01J2219/00873 , B01J2219/00891 , B01L3/5027 , B01L3/502715 , B01L2300/0809 , B01L2300/0861 , B01L2300/0883 , G01N2035/00366 , G01N2035/00514
摘要： A method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.
摘要翻译：用于扩增核酸的方法和装置。该方法包括通过不同的入口通道将含有用于核酸扩增的反应物的反应物水溶液和与反应物水溶液相分离并且不参与扩增反应的流体引入反应容器，形成多个反应物水溶液通过使反应物水溶液与反应容器中的流体接触，并且扩增反应物水溶液液滴中的核酸，由流体包围的液滴。该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器，以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

86. 发明申请

US20090317918A1 METHOD OF HEATING LIQUID MEDIUM USING MICROWAVES AND ANIONS 审中-公开
标题翻译：使用微波和阴离子加热液体介质的方法
公开(公告)号：US20090317918A1
公开(公告)日：2009-12-24
申请号：US12553402
申请日：2009-09-03
申请人： Kyu-youn Hwang , Joon-ho Kim , Koong-kak Nam , Won-seok Chung , Hee-kyun Lim
发明人： Kyu-youn Hwang , Joon-ho Kim , Koong-kak Nam , Won-seok Chung , Hee-kyun Lim
IPC分类号： G01N1/00 , G01N33/48 , C12M1/00
CPC分类号： B01L3/502715 , B01L2200/16 , B01L2300/0806 , B01L2300/0816 , B01L2300/087 , B01L2300/1833 , B01L2300/1866 , B01L2400/0409 , Y10T436/25125
摘要： Provided is a method comprising adding anions having a high charge density to a liquid medium; the liquid medium comprising molecules that hydrogen bond with one another; the anions interacting with the molecules of the liquid medium with a force that is stronger than the forces that produce hydrogen bonding between the molecules of the medium; and heating the liquid medium by irradiating it with microwaves.
摘要翻译：提供了一种方法，包括向液体介质中加入具有高电荷密度的阴离子; 所述液体介质包含彼此氢键合的分子; 阴离子与液体介质的分子相互作用，其力比在介质分子之间产生氢键的力更强; 并通过用微波照射来加热液体介质。

87. 发明申请

US20090131649A1 METHOD OF SEPARATING SMALL RNA MOLECULES USING KOSMOTROPIC SALT 有权
标题翻译：使用KOSMOTROPIC盐分离小RNA分子的方法
公开(公告)号：US20090131649A1
公开(公告)日：2009-05-21
申请号：US12110373
申请日：2008-04-28
申请人： Myo-yong LEE , Nam Huh , Joon-ho Kim
发明人： Myo-yong LEE , Nam Huh , Joon-ho Kim
IPC分类号： C07H1/00
CPC分类号： C12N15/1006
摘要： Disclosed is a method of separating small RNAs of 200 nucleotides or less from larger RNAs on a solid support, using a kosmotropic salt of different concentrations.
摘要翻译：公开了一种使用不同浓度的可变性盐分离从固体支持物上的较大RNA分离200个核苷酸或更少的小RNA的方法。

88. 发明申请

US20090048437A1 METHOD OF PURIFYING RNA USING KOSMOTROPIC SALT 有权
标题翻译：使用KOSMOTROPIC盐净化RNA的方法
公开(公告)号：US20090048437A1
公开(公告)日：2009-02-19
申请号：US12110391
申请日：2008-04-28
申请人： Myo-yong LEE , Nam Huh , Joon-ho Kim
发明人： Myo-yong LEE , Nam Huh , Joon-ho Kim
IPC分类号： C07H21/02
CPC分类号： C07H21/02 , C12N15/1003 , C12N15/1006
摘要： The present invention provides a method of purifying RNA, including contacting a solid support with an acidic solution having a RNA-containing sample and a kosmotropic salt having a concentration of less than 1M, thereby binding the RNA to the solid support. According to the present invention, RNA is purified efficiently due to high RNA yield and low contamination by DNA. The present invention is particularly effective in purifying RNAs of 200 nucleotides or less.
摘要翻译：本发明提供了一种纯化RNA的方法，包括使固体支持物与具有含RNA样品的酸性溶液和浓度小于1M的感应性盐接触，从而将RNA与固体支持物结合。根据本发明，由于高RNA产量和低DNA污染，RNA被有效地纯化。本发明在纯化200个或更少核苷酸的RNA中特别有效。

89. 发明授权

US07488596B2 Microfluidic device comprising electrolysis device for cell lysis and method for electrochemically lysing cells using the same 有权
标题翻译：微流体装置，其包括用于细胞裂解的电解装置和使用其的电化学裂解细胞的方法
公开(公告)号：US07488596B2
公开(公告)日：2009-02-10
申请号：US11304083
申请日：2005-12-15
申请人： Hun-joo Lee , Joon-ho Kim , Chang-eun Yoo , Hee-kyun Lim , Kyu-youn Hwang , Soo-min Ma , Jun-hong Min
发明人： Hun-joo Lee , Joon-ho Kim , Chang-eun Yoo , Hee-kyun Lim , Kyu-youn Hwang , Soo-min Ma , Jun-hong Min
IPC分类号： C12M1/33 , C12M1/34 , C12Q1/70 , C12N1/06
CPC分类号： C12N1/066 , C12M47/06
摘要： Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.
摘要翻译：提供了一种微流体装置，其包括用于细胞裂解的电解装置，其包括阳极室，阴极室和隔板，其中隔膜安装在阳极室和阴极室之间，阳极室包括入口和出口阳极室溶液和电极，阴极室包括用于阴极室溶液和电极的入口和出口，以及使用其进行电化学裂解细胞的方法。

90. 发明授权

US07151301B2 Sensitivity enhanced biomolecule field effect transistor 有权
标题翻译：灵敏度增强的生物分子场效应晶体管
公开(公告)号：US07151301B2
公开(公告)日：2006-12-19
申请号：US11057642
申请日：2005-02-14
申请人： Kyu-tae Yoo , Geun-bae Lim , Joon-ho Kim , Kak Namkoong
发明人： Kyu-tae Yoo , Geun-bae Lim , Joon-ho Kim , Kak Namkoong
IPC分类号： H01L29/76
CPC分类号： G01N27/4145
摘要： There is provided a biomolecule FET enhancing a sensitivity. The biomolecule FET includes a substrate, first and second impurity regions formed on both sides of the substrate, and doped with impurities of a polarity opposite to that of the substrate, a gate formed on the substrate and being in contact with the first and second impurity regions, and a probe biomolecule attached to the gate. A region of the gate adjacent to the first impurity region is wider than a region thereof adjacent to the second impurity region. A density of the probe biomolecule attached to the surface of the gate is increased, and when detecting a level of hybridization of the probe biomolecule and the target biomolecule, its sensitivity is improved.
摘要翻译：提供了提高灵敏度的生物分子FET。生物分子FET包括基板，形成在基板两侧的第一和第二杂质区，并且掺杂极性与基板极性相反的杂质;形成在基板上并与第一和第二杂质接触的栅极区域，以及连接到门的探针生物分子。与第一杂质区相邻的栅极的区域比与第二杂质区相邻的区域宽。附着在栅极表面的探针生物分子的密度增加，并且当检测到探针生物分子和靶生物分子的杂交水平时，其灵敏度得到改善。

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