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71. 发明申请

US20130233501A1 MODIFICATION OF XYLAN 审中-公开
标题翻译： XYLAN的修改
公开(公告)号：US20130233501A1
公开(公告)日：2013-09-12
申请号：US13703223
申请日：2011-06-07
申请人： Willem Heber Van Zyl , Annie Fabian Abel Chimphango , Johann Ferdinand Gorgens
发明人： Willem Heber Van Zyl , Annie Fabian Abel Chimphango , Johann Ferdinand Gorgens
IPC分类号： D21H17/24
CPC分类号： D21H17/24 , C09D105/14 , C12P19/04 , C12P2203/00 , C12Y302/01055 , C12Y302/01139 , D21C9/005 , D21H11/20
摘要： The invention provides a method of modifying soluble xylan which contains glucuronic acid and/or arabinose side chains so that it can be adsorbed onto other substrates. The method includes the steps of enzymatically modifying the xylan by selectively removing glucuronic acid and/or arabinose side chains from the xylan with α-D-glucuronidase and/or α-L-arabinofuranosidase, and allowing the modified xylan to adsorb onto the substrate. The substrate may be a cellulosic substrate like pulp or paper or a non-cellulosic substrate. The enzymes are able to remove a sufficient number of the xylan side chains so as to decrease water solubility of the xylan, induce xylan precipitation and allow adsorption of the xylan onto other substrates. A pulping process which incorporates modification of xylan and adsorption onto cellulosic material is also provided, as are products which contain the modified xylan.
摘要翻译：本发明提供了一种修饰含有葡糖醛酸和/或阿拉伯糖侧链的可溶性木聚糖的方法，使其可以吸附到其它底物上。该方法包括以下步骤：通过用α-D-葡糖醛酸糖苷酶和/或α-L-阿拉伯呋喃糖苷酶选择性地除去来自木聚糖的葡糖醛酸和/或阿拉伯糖侧链，并且使改性的木聚糖吸附在基材上，酶促改性木聚糖。基材可以是纤维素基质如纸浆或纸或非纤维素基质。酶能够除去足够数量的木聚糖侧链，以降低木聚糖的水溶性，诱导木聚糖沉淀并允许木聚糖吸附到其它底物上。还提供了将木聚糖修饰和吸附到纤维素材料上的制浆方法，以及含有改性木聚糖的产品。

72. 发明申请

US20130177947A1 NOVEL GLYCOSYL HYDROLASE ENZYMES AND USES THEREOF 审中-公开
标题翻译：新型糖苷酶水解酶及其用途
公开(公告)号：US20130177947A1
公开(公告)日：2013-07-11
申请号：US13498069
申请日：2010-09-22
申请人： Benjamin Bower , Megan Yee Hsi , Thijs Kaper , Bradley R. Keleman , Suzanne E. Lantz , Edmund A. Larenas , Colin Mitchinson , Steven Kim , William D. Hitz , Mark Emptage , Keith Dumont Wing
发明人： Benjamin Bower , Megan Yee Hsi , Thijs Kaper , Bradley R. Keleman , Suzanne E. Lantz , Edmund A. Larenas , Colin Mitchinson , Steven Kim , William D. Hitz , Mark Emptage , Keith Dumont Wing
IPC分类号： C12N9/24 , C12P19/14 , C12N9/16 , C12N9/20 , C12N9/00
CPC分类号： C12N9/248 , C12N9/0006 , C12N9/0065 , C12N9/16 , C12N9/20 , C12N9/2402 , C12N9/2437 , C12N9/2445 , C12N9/2485 , C12P19/00 , C12P19/02 , C12P19/14 , C12Y302/01 , C12Y302/01004 , C12Y302/01008 , C12Y302/01021 , C12Y302/01037 , C12Y302/01055 , Y02E50/343
摘要： The present disclosure is generally directed to glycosyl hydrolase enzymes, compositions comprising such enzymes, and methods of using the enzymes and compositions, for example for the saccharification of cellulosic and hemicellulosic materials into sugars.
摘要翻译：本公开一般涉及糖基水解酶，包含这些酶的组合物，以及使用酶和组合物的方法，例如将纤维素和半纤维素材料糖化为糖。

73. 发明授权

US08202716B2 Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods 有权
公开(公告)号：US08202716B2
公开(公告)日：2012-06-19
申请号：US12927490
申请日：2010-11-15
申请人： David N. Thompson , William A. Apel , Vicki S. Thompson , David W. Reed , Jeffrey A. Lacey , Emily D. Henriksen
发明人： David N. Thompson , William A. Apel , Vicki S. Thompson , David W. Reed , Jeffrey A. Lacey , Emily D. Henriksen
IPC分类号： C12N9/42 , C12N9/14 , C12N9/00 , C12P21/06 , C08B37/00 , D21C1/00 , C02F3/34 , C07H21/04 , C07K1/00
CPC分类号： C12Y302/01055 , C12N9/2402 , C12N15/52 , Y02W10/45
摘要： Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.

74. 发明授权

US08101393B2 Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them 有权
标题翻译：纤维素分解酶，编码它们的核酸和制备和使用它们的方法
公开(公告)号：US08101393B2
公开(公告)日：2012-01-24
申请号：US12278958
申请日：2006-12-08
申请人： Kevin A. Gray , Lishan Zhao , Michelle H. Cayouette
发明人： Kevin A. Gray , Lishan Zhao , Michelle H. Cayouette
IPC分类号： C12N9/42 , C12N15/00 , C12P7/10 , C12P21/06 , C07H21/04 , C07H5/06 , C07K1/00 , A61K38/47 , A23L2/00 , A23L1/202 , A23L1/30
CPC分类号： C12N9/2437 , C12N9/2402 , C12N9/2405 , C12N9/248 , C12Y302/01004 , C12Y302/01006 , C12Y302/01008 , C12Y302/01021 , C12Y302/01023 , C12Y302/01039 , C12Y302/01055 , C12Y302/01091 , Y02E50/16 , Y02E50/17 , Y10T428/249921
摘要： The invention provides polypeptides having any cellulolytic activity, e.g., a cellulase activity, a endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a β-xylosidase, an arabinofuranosidase, and/or an oligomerase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, β-xylosidase, arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomers to fermentable sugars in the saccharification of biomass. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. The invention also provides compositions or products of manufacture comprising mixtures of enzymes comprising at least one enzyme of this invention.
摘要翻译：本发明提供了具有任何纤维素分解活性的多肽，例如纤维素酶活性，内切葡聚糖酶，纤维二糖水解酶，β-葡糖苷酶，木聚糖酶，甘露聚糖酶，β-半乳糖苷酶，阿拉伯呋喃糖苷酶和/或寡聚酶活性，编码这些多肽，以及制备和使用这些多核苷酸和多肽的方法。在一个方面，本发明涉及具有任何纤维素分解活性的多肽，例如纤维素酶活性，例如内切葡聚糖酶，纤维二糖水解酶，β-葡糖苷酶，木聚糖酶，甘露聚糖，β-木糖苷酶，阿拉伯呋喃糖苷酶和/或寡聚酶活性，包括热稳定性和耐热活性，以及编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。一方面，本发明提供了具有寡聚酶活性的多肽，例如在生物质糖化中将顽固性可溶性寡聚物转化为可发酵糖的酶。本发明的多肽可用于各种药物，农业，食品和饲料加工以及工业环境中。本发明还提供包含本发明的至少一种酶的酶的混合物的组合物或制品。

75. 发明申请

US20120015407A1 Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods 有权
标题翻译：嗜热和热嗜酸性生物聚合物降解基因和酸性脂肪酸杆菌和相关生物的酶，方法
公开(公告)号：US20120015407A1
公开(公告)日：2012-01-19
申请号：US12927490
申请日：2010-11-15
申请人： David N. Thompson , William A. Apel , Vicki S. Thompson , David W. Reed , Jeffrey A. Lacey , Emily D. Henriksen
发明人： David N. Thompson , William A. Apel , Vicki S. Thompson , David W. Reed , Jeffrey A. Lacey , Emily D. Henriksen
IPC分类号： C12N9/24 , C12P19/14
CPC分类号： C12Y302/01055 , C12N9/2402 , C12N15/52 , Y02W10/45
摘要： Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.
摘要翻译：提供了分离和/或纯化的多肽和编码来自酸热环酸杆菌的多肽的核酸序列。还提供了使用分离的至少部分降解，切割或除去多糖，木质纤维素，纤维素，半纤维素，木质素，淀粉，几丁质，聚羟基丁酸酯，异木聚糖，糖苷，木聚糖，葡聚糖，半乳聚糖或甘露聚糖装饰基团的方法和/或编码来自酸性脂多酸杆菌的多肽的纯化多肽和核酸序列。

76. 发明申请

US20110059487A1 ENGINEERED MICROORGANISMS AND METHODS OF USE 审中-公开
标题翻译：工程微生物及其使用方法
公开(公告)号：US20110059487A1
公开(公告)日：2011-03-10
申请号：US12822765
申请日：2010-06-24
申请人： Kevin A. Jarrell , Gabriel Reznik , Michelle A. Pynn
发明人： Kevin A. Jarrell , Gabriel Reznik , Michelle A. Pynn
IPC分类号： C12P21/00 , C12N1/00 , C12N1/21 , C12P1/00 , C12P13/04
CPC分类号： C12P21/02 , C12N1/20 , C12N9/0065 , C12N9/18 , C12N9/2465 , C12Y301/01006 , C12Y302/01004 , C12Y302/01008 , C12Y302/01021 , C12Y302/01022 , C12Y302/01037 , C12Y302/01055 , C12Y302/01074 , C12Y302/01086 , C12Y302/01091 , C12Y302/01139
摘要： The present invention provides, among other things, engineered microorganisms and methods that allow efficient conversion of soy carbohydrates to industrial chemicals by fermentation. In some embodiments, the invention provides microbial cells engineered to have increased efficiency in utilizing a soy carbon source (e.g., soy molasses, soy meal, and/or soy hulls) as compared to a parent cell. In some embodiments, microbial cells are engineered to have altered (e.g., increased) expression or activity of one or more carbohydrate modifying enzymes (e.g., glycosidases). In some embodiments, microbial cells are engineered to have altered localization of carbohydrate modifying enzymes (e.g., glycosidases). In some embodiments, engineered microbial cells provided herein are used to produce industrial chemicals (e.g., surfactin) using soy components as primary or sole carbon sources.
摘要翻译：本发明尤其提供了工程微生物和允许通过发酵将大豆碳水化合物有效转化成工业化学品的方法。在一些实施方案中，与亲本细胞相比，本发明提供了工程化以提高利用大豆碳源（例如大豆糖蜜，大豆粉和/或大豆皮）的效率的微生物细胞。在一些实施方案中，将微生物细胞工程化以改变（例如增加）一种或多种碳水化合物修饰酶（例如糖苷酶）的表达或活性。在一些实施方案中，将微生物细胞工程化以改变碳水化合物修饰酶（例如糖苷酶）的定位。在一些实施方案中，本文提供的工程微生物细胞用于使用大豆成分作为主要或唯一碳源来生产工业化学品（例如表面活性素）。

77. 发明申请

US20100311110A1 Thermal and acid tolerant beta xylosidases, arabinofuranosidases, genes encoding, related organisms, and methods 有权
标题翻译：热和酸耐受性β木糖苷酶，阿拉伯呋喃糖苷酶，编码相关生物的基因和方法
公开(公告)号：US20100311110A1
公开(公告)日：2010-12-09
申请号：US12802911
申请日：2010-06-15
申请人： David N. Thompson , Vicki S. Thompson , Kastli D. Schaller , William A. Apel , David W. Reed , Jeffrey A. Lacey
发明人： David N. Thompson , Vicki S. Thompson , Kastli D. Schaller , William A. Apel , David W. Reed , Jeffrey A. Lacey
IPC分类号： C12P1/00 , C12N9/26 , C07H21/00 , C12N9/00 , C12N15/63
CPC分类号： C12Y302/01037 , C12N9/2402 , C12N9/248 , C12N9/2482 , C12Y302/01055 , D21C5/005 , D21C11/0007
摘要： Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius and variations thereof are provided. Further provided are methods of at least partially degrading xylotriose, xylobiose, and/or arabinofuranose-substituted xylan using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius and variations thereof.
摘要翻译：提供了分离和/或纯化的多肽和编码来自酸热环酸杆菌的多肽的核酸序列及其变体。还提供了使用分离和/或纯化的多肽和编码来自酸碱热杆菌的多肽的核酸序列及其变体来至少部分降解木三糖，木二糖和/或阿拉伯呋喃糖取代的木聚糖的方法。

78. 发明申请

US20090136476A1 ARABINOFURANOSIDASES 审中-公开
标题翻译：阿拉伯糖尿病
公开(公告)号：US20090136476A1
公开(公告)日：2009-05-28
申请号：US11911683
申请日：2006-04-25
申请人： Hanne Risbjerg Soerensen , Christel Thea Joergensen , Lars Hylling Christensen , Christian Isak Joergensen , Carsten Hoerslev Hansen , Lene Venke Kofod
发明人： Hanne Risbjerg Soerensen , Christel Thea Joergensen , Lars Hylling Christensen , Christian Isak Joergensen , Carsten Hoerslev Hansen , Lene Venke Kofod
IPC分类号： A61K38/46 , C12N9/14 , C07H21/04 , C12N15/63 , C12N1/21 , C12P21/06
CPC分类号： C12N9/2402 , A21D8/042 , A23K20/168 , A23L29/06 , C12Y302/01055 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to isolated polypeptides having alpha-L-arabinofuranosidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
摘要翻译：本发明涉及具有α-L-阿拉伯呋喃糖苷酶活性的分离的多肽和分离的编码多肽的核酸序列。本发明还涉及包含核酸序列的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

79. 发明申请

US20060094031A1 Alpha-L-arabinofuranosidase histochemical reporter gene assay 审中-公开
标题翻译： α-阿拉伯呋喃糖苷酶组织化学报告基因测定
公开(公告)号：US20060094031A1
公开(公告)日：2006-05-04
申请号：US11066633
申请日：2005-02-25
申请人： Brian Sauer
发明人： Brian Sauer
IPC分类号： C12Q1/68 , C07H21/04 , C12N1/21 , C12N5/06 , C12N1/18
CPC分类号： C12N9/2471 , C07H17/02 , C12N9/2402 , C12Q1/34 , C12Y302/01023 , C12Y302/01055 , G01N2333/924
摘要： The present invention provides a novel reporter gene assay. In particular, the present invention provides a histochemical reporter gene assay employing α-L-arabinofuranosidase in combination with a chromogenic α-L-arabinofuranosidase substrate. The α-L-arabinofuranosidase histochemical reporter assay may be utilized to characterize gene regulatory regions of interest either alone or in combination with other assays.
摘要翻译：本发明提供了一种新的报道基因测定法。特别地，本发明提供组合化学报告基因测定法，其使用α-L - 阿拉伯呋喃糖苷酶与显色性α-L-阿拉伯呋喃糖苷酶底物组合。 α-L - 阿拉伯呋喃糖苷酶组织化学报告物测定可用于单独描述感兴趣的基因调节区或与其它测定组合。

80. 发明申请

US20030175893A1 Novel method to isolate mutants and to clone the complementing gene 有权
标题翻译：分离突变体和克隆补体基因的新方法
公开(公告)号：US20030175893A1
公开(公告)日：2003-09-18
申请号：US10419969
申请日：2003-04-22
申请人： DANSICO INGREDIENTS A/S
发明人： Leendert Hendrik De Graaff , Henrietta Catharina Van Den Broeck , Jacob Visser
IPC分类号： C12P021/02 , C07H021/04 , C12N001/16 , C12N015/74 , C07K014/38
CPC分类号： C12N15/635 , C07K14/38 , C07K14/4705 , C12N9/0006 , C12N9/2434 , C12N9/248 , C12N9/88 , C12N15/1027 , C12N15/63 , C12N15/65 , C12N15/80 , C12Q1/02 , C12Y302/01003 , C12Y302/01008 , C12Y302/01015 , C12Y302/01037 , C12Y302/01055 , C12Y302/01099
摘要： The subject invention lies in the field of microorganism mutation and selection of the mutants. In particular the invention is directed at obtaining metabolic mutants in a simple, direct and specific manner. In a preferred embodiment it is also possible to obtain desired mutants not comprising recombinant DNA, thereby facilitating incorporation thereof in products for human consumption or application, due to shorter legislative procedures. The method according to the invention involves random mutation and specific selection of the desired metabolic mutant. A nucleic acid cassette comprising a nucleic acid sequence encoding a bidirectional marker, said nucleic acid cassette further comprising a basic transcriptional unit operatively linked to the nucleic acid sequence encoding the bidirectional marker and said nucleic acid cassette further comprising an inducible enhancer or activator sequence linked to the basic transcription unit in such a manner that upon induction of the enhancer or activator sequence the bidirectional marker encoding nucleic acid sequence is expressed, said inducible enhancer or activator sequence being derived from a gene associated with activity of part of metabolism, said inducible enhancer or activator sequence being derived from a gene associated with metabolism is claimed as is application thereof in a selection method for mutants. In addition a regulator gene xlnR encoding an activating regulator of an inducible enhancer or activator sequence and application of said gene and/or its expression product in overexpression of homologous or heterologous protein or peptide is described. Knockout mutants wherein said gene is absent or inactivated and mutants with increased or decreased DNA binding capacity are also claimed.
摘要翻译：本发明在于微生物突变和突变体的选择领域。特别地，本发明涉及以简单，直接和具体的方式获得代谢突变体。在优选的实施方案中，由于较短的立法程序，也可以获得不包含重组DNA的所需突变体，从而便于将其并入用于人类消费或应用的产品中。根据本发明的方法涉及随机突变和所需代谢突变体的特异性选择。一种核酸盒，其包含编码双向标记的核酸序列，所述核酸盒还包含与编码双向标记的核酸序列可操作地连接的碱性转录单位，并且所述核酸盒还包含可诱导增强子或活化剂序列所述基本转录单元以这样的方式，即在诱导增强子或活化剂序列时，表达编码双向标记核酸序列的核酸序列，所述诱导型增强子或活化子序列衍生自与部分代谢活性相关的基因，所述诱导型增强子或要求从与代谢相关的基因衍生的活化剂序列作为其在突变体的选择方法中的应用。此外，描述了编码诱导型增强子或激活物序列的活化调节物的调节基因xlnR以及所述基因和/或其表达产物在同源或异源蛋白质或肽的过表达中的应用。其中所述基因不存在或失活的敲除突变体以及DNA结合能力增加或降低的突变体也被要求保护。

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