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1. 发明申请

US20130233501A1 MODIFICATION OF XYLAN 审中-公开
标题翻译： XYLAN的修改
公开(公告)号：US20130233501A1
公开(公告)日：2013-09-12
申请号：US13703223
申请日：2011-06-07
申请人： Willem Heber Van Zyl , Annie Fabian Abel Chimphango , Johann Ferdinand Gorgens
发明人： Willem Heber Van Zyl , Annie Fabian Abel Chimphango , Johann Ferdinand Gorgens
IPC分类号： D21H17/24
CPC分类号： D21H17/24 , C09D105/14 , C12P19/04 , C12P2203/00 , C12Y302/01055 , C12Y302/01139 , D21C9/005 , D21H11/20
摘要： The invention provides a method of modifying soluble xylan which contains glucuronic acid and/or arabinose side chains so that it can be adsorbed onto other substrates. The method includes the steps of enzymatically modifying the xylan by selectively removing glucuronic acid and/or arabinose side chains from the xylan with α-D-glucuronidase and/or α-L-arabinofuranosidase, and allowing the modified xylan to adsorb onto the substrate. The substrate may be a cellulosic substrate like pulp or paper or a non-cellulosic substrate. The enzymes are able to remove a sufficient number of the xylan side chains so as to decrease water solubility of the xylan, induce xylan precipitation and allow adsorption of the xylan onto other substrates. A pulping process which incorporates modification of xylan and adsorption onto cellulosic material is also provided, as are products which contain the modified xylan.
摘要翻译：本发明提供了一种修饰含有葡糖醛酸和/或阿拉伯糖侧链的可溶性木聚糖的方法，使其可以吸附到其它底物上。该方法包括以下步骤：通过用α-D-葡糖醛酸糖苷酶和/或α-L-阿拉伯呋喃糖苷酶选择性地除去来自木聚糖的葡糖醛酸和/或阿拉伯糖侧链，并且使改性的木聚糖吸附在基材上，酶促改性木聚糖。基材可以是纤维素基质如纸浆或纸或非纤维素基质。酶能够除去足够数量的木聚糖侧链，以降低木聚糖的水溶性，诱导木聚糖沉淀并允许木聚糖吸附到其它底物上。还提供了将木聚糖修饰和吸附到纤维素材料上的制浆方法，以及含有改性木聚糖的产品。

2. 发明申请

US20130089592A1 METHOD OF PRODUCING A HYDROGEL 审中-公开
标题翻译：生产水凝胶的方法
公开(公告)号：US20130089592A1
公开(公告)日：2013-04-11
申请号：US13703228
申请日：2011-06-07
申请人： Willem Heber Van Zyl , Annie Fabian Abel Chimphango , Johann Ferdinand Gorgens
发明人： Willem Heber Van Zyl , Annie Fabian Abel Chimphango , Johann Ferdinand Gorgens
IPC分类号： A61K47/36 , A61K9/48 , C12P19/04 , A61K38/44
CPC分类号： A61K47/36 , A61K9/06 , A61K9/4833 , A61K9/5161 , A61K38/44 , B82Y5/00 , C08L5/14 , C12N11/04 , C12N11/10 , C12P19/04 , C12P19/14 , Y10S977/906
摘要： The invention provides a method of enzymatically modifying xylan by selectively removing glucuronic acid and/or arabinose side chains from the xylan with a-D-glucuronidase and/or a-L-arabinofuranosidase, and allowing the modified xylan to form into a hydrogel when the xylan becomes insoluble. A bioactive substance, such as a protein, enzyme, antimicrobial agent, bactericide or pharmaceutical compound can be added to the xylan so that the substance is encapsulated within the hydrogel or incorporated onto its surface. The hydrogel can be used as a drug delivery agent, such as for sustained-release or targeted drug delivery, rectal drug delivery or a dressing for a wound, burn or scar. The hydrogel can also be used as a coating, such as on medical gloves, catheters, surgical drainage systems, utensils or the like, or can be used in a scaffold for tissue engineering.
摘要翻译：本发明提供了一种通过用α-葡萄糖醛酸酶和/或α-阿拉伯呋喃糖苷酶选择性地除去来自木聚糖的葡糖醛酸和/或阿拉伯糖侧链来酶促修饰木聚糖的方法，并且当木聚糖变得不溶时，允许改性的木聚糖形成水凝胶。可以将生物活性物质如蛋白质，酶，抗微生物剂，杀菌剂或药物化合物加入到木聚糖中，使得物质被包封在水凝胶内或并入其表面。水凝胶可用作药物递送剂，例如用于缓释或靶向药物递送，直肠药物递送或用于伤口，烧伤或瘢痕的敷料。水凝胶也可以用作涂层，例如在医用手套，导管，手术排液系统，器具等上，或者可以用于组织工程的支架中。

3. 发明申请

US20110177542A1 METHOD FOR ENHANCING CELLOBIOSE UTILIZATION 有权
标题翻译：增强细胞利用的方法
公开(公告)号：US20110177542A1
公开(公告)日：2011-07-21
申请号：US12599425
申请日：2008-05-09
申请人： Willem Heber Van zyl , Ronel Van Rooyen , Shaunita Hellouise Rose
发明人： Willem Heber Van zyl , Ronel Van Rooyen , Shaunita Hellouise Rose
IPC分类号： C12P7/10 , C12N1/19 , C12N1/15 , C12P1/02 , C12Q1/02
CPC分类号： C12P7/10 , C12N1/36 , C12N9/2445 , C12Y302/01021 , Y02E50/16 , Y02P20/52
摘要： The present invention relates to methods for improving a host cell's ability to utilize the disaccharide cellobiose. In some embodiments, a transformed cell expresses intracellular β-glucosidase. In other embodiments, a transformed host cell is able to grow on media wherein cellobiose is the sole carbon source. In other embodiments, selection methods are provided which improve a host cell's ability to grow on cellobiose-containing media.
摘要翻译：本发明涉及提高宿主细胞利用二糖纤维二糖的能力的方法。在一些实施方案中，转化细胞表达细胞内葡萄糖苷酶。在其它实施方案中，转化的宿主细胞能够在纤维二糖是唯一碳源的培养基上生长。在其它实施方案中，提供了提高宿主细胞在含纤维二糖的培养基上生长能力的选择方法。

4. 发明申请

US20130244295A1 Biofuel Production 有权
标题翻译：生物燃料生产
公开(公告)号：US20130244295A1
公开(公告)日：2013-09-19
申请号：US13640902
申请日：2010-04-12
申请人： Willem Heber Van Zyl , Tania Jooste , Johann Ferdinand Gorgens , Maryna Saayman , Lorenzo Favaro , Marina Basaglia , Sergio Casella
发明人： Willem Heber Van Zyl , Tania Jooste , Johann Ferdinand Gorgens , Maryna Saayman , Lorenzo Favaro , Marina Basaglia , Sergio Casella
IPC分类号： C12N15/81 , C12P7/06
CPC分类号： C12N15/81 , C12N9/2402 , C12N9/242 , C12N9/2428 , C12P7/06 , C12P19/14 , Y02E50/17
摘要： The invention relates to the combination of the TLG1 glucoamylase from Thermomyces lanuginoses with: (i) the SFA1 alpha-amylase from Saccharomycopsis fibuligera; and/or (ii) the LKA alpha-amylase. The enzyme combinations may be expressed in a host cell (e.g. in Saccharomyces cerevisiae) or provided as an enzyme composition. Methods for making the enzyme combinations of the invention are provided. The invention also relates to a yeast strain which exhibits amylolytic activity and promising fermentative abilities. Processes for producing a fermentation product (in particular alcohol) from starch-containing material are described.
摘要翻译：本发明涉及来自Thermomyces lanuginoses的TLG1葡糖淀粉酶与（i）来自Saccharomycopsis fibuligera的SFA1α-淀粉酶的组合; 和/或（ii）LKAα-淀粉酶。酶组合可以在宿主细胞（例如酿酒酵母）中表达或作为酶组合物提供。提供了制备本发明的酶组合的方法。本发明还涉及显示淀粉分解活性和有希望的发酵能力的酵母菌株。描述了从含淀粉材料生产发酵产物（特别是醇）的方法。

5. 发明申请

US20130185037A1 System for Modelling the Conversion of Lignocellulosic Materials 审中-公开
标题翻译：木质纤维素材料转化建模系统
公开(公告)号：US20130185037A1
公开(公告)日：2013-07-18
申请号：US13808592
申请日：2011-07-08
申请人： Willem Heber Van Zyl , Josebus Maree Van Zyl , Thomas Michael Harms , Engene Van Rensburg , Lee Rybeck Lynd
发明人： Willem Heber Van Zyl , Josebus Maree Van Zyl , Thomas Michael Harms , Engene Van Rensburg , Lee Rybeck Lynd
IPC分类号： G06F19/00
CPC分类号： G16C20/10 , C12P7/10 , Y02E50/16
摘要： A system for modelling the conversion of crystalline insoluble cellulose to ethanol is provided which includes a processor configured to calculate the production rate for ethanol based on a number of inputs and as a function of specific equations. The system can form part of a control system for controlling the operation of a plant which produces ethanol from cellulose.
摘要翻译：提供了一种用于模拟结晶不溶性纤维素向乙醇的转化的系统，其包括处理器，其配置为基于输入数量和作为特定方程式的函数计算乙醇的生产速率。该系统可以形成用于控制从纤维素产生乙醇的设备的操作的控制系统的一部分。

6. 发明申请

US20110129888A1 Method for Fermenting Cellulosics 审中-公开
标题翻译：发酵纤维素的方法
公开(公告)号：US20110129888A1
公开(公告)日：2011-06-02
申请号：US12677956
申请日：2007-09-14
申请人： Willem Heber Van Zyl , Riaan Den Haan
发明人： Willem Heber Van Zyl , Riaan Den Haan
IPC分类号： C12P7/10 , C12N1/19
CPC分类号： C12P7/10 , Y02E50/16
摘要： The present invention is directed to host cells capable of fermenting cellulosic materials for the production of ethanol. Microorganisms engineered to be able to use amorphous cellulosic materials in a fermentation process to produce ethanol are disclosed. Additionally, methods of using the host organisms of the invention and compositions for producing ethanol according to the invention are disclosed.
摘要翻译：本发明涉及能够发酵纤维素材料以生产乙醇的宿主细胞。公开了在发酵过程中能够使用无定形纤维素材料来生产乙醇的微生物。此外，公开了使用本发明的宿主生物和根据本发明的用于生产乙醇的组合物的方法。

7. 发明申请

US20090004331A1 Process of Producing Rooibos Tea Extract 审中-公开
标题翻译：生产Rooibos茶提取物的过程
公开(公告)号：US20090004331A1
公开(公告)日：2009-01-01
申请号：US11658439
申请日：2005-07-25
申请人： Marinda Bloom , Willem Heber Van Zyl , Elizabeth Joubert , Alfred Botha , Dawie De Villiers
发明人： Marinda Bloom , Willem Heber Van Zyl , Elizabeth Joubert , Alfred Botha , Dawie De Villiers
IPC分类号： A23F3/10
CPC分类号： A23F3/34
摘要： The invention provides a process of treating Rooibos plant material to obtain Rooibos tea extracts with improved extract yield and colour development. The process includes contacting Rooibos plant material with an effective amount of at least one exogenous enzyme under predetermined conditions thereby to obtain Rooibos tea extracts having improved extract yield and colour development. The enzyme is selected from pectinase, ferulic acid esterase, β-glucanase, cellobiohydrolase, β-xylanase and phenol oxidase and the Rooibos plant material is selected from green tea, fermented tea or spent tea.
摘要翻译：本发明提供了一种治疗Rooibos植物材料以获得具有改善的提取物产量和颜色发育的Rooibos茶提取物的方法。该方法包括在预定条件下将Rooibos植物材料与有效量的至少一种外源酶接触，从而获得具有提高的提取物产量和显色性能的Rooibos茶提取物。该酶选自果胶酶，阿魏酸酯酶，β-葡聚糖酶，纤维二糖水解酶，β-木聚糖酶和酚氧化酶，Rooibos植物材料选自绿茶，发酵茶或废茶。

8. 发明授权

US09243256B2 Biofuel production 有权
标题翻译：生物燃料生产
公开(公告)号：US09243256B2
公开(公告)日：2016-01-26
申请号：US13640902
申请日：2010-04-12
申请人： Willem Heber Van Zyl , Tania Jooste , Johann Ferdinand Gorgens , Maryna Saayman , Lorenzo Favaro , Marina Basaglia , Sergio Casella
发明人： Willem Heber Van Zyl , Tania Jooste , Johann Ferdinand Gorgens , Maryna Saayman , Lorenzo Favaro , Marina Basaglia , Sergio Casella
IPC分类号： C12N1/14 , C12N9/30 , C12N9/34 , C12N15/00 , C12N1/20 , C12P7/00 , C07H21/04 , C12N15/81 , C12N9/24 , C12P7/06 , C12P19/14
CPC分类号： C12N15/81 , C12N9/2402 , C12N9/242 , C12N9/2428 , C12P7/06 , C12P19/14 , Y02E50/17
摘要： The invention relates to the combination of the TLG1 glucoamylase from Thermomyces lanuginoses with: (i) the SFA1 alpha-amylase from Saccharomycopsis fibuligera; and/or (ii) the LKA alpha-amylase. The enzyme combinations may be expressed in a host cell (e.g. in Saccharomyces cerevisiae) or provided as an enzyme composition. Methods for making the enzyme combinations of the invention are provided. The invention also relates to a yeast strain which exhibits amylolytic activity and promising fermentative abilities. Processes for producing a fermentation product (in particular alcohol) from starch-containing material are described.
摘要翻译：本发明涉及来自Thermomyces lanuginoses的TLG1葡糖淀粉酶与（i）来自Saccharomycopsis fibuligera的SFA1α-淀粉酶的组合; 和/或（ii）LKAα-淀粉酶。酶组合可以在宿主细胞（例如酿酒酵母）中表达或作为酶组合物提供。提供了制备本发明的酶组合的方法。本发明还涉及显示淀粉分解活性和有希望的发酵能力的酵母菌株。描述了从含淀粉材料生产发酵产物（特别是醇）的方法。

9. 发明授权

US06475768B1 Xylose isomerase with improved properties 失效
标题翻译：具有改进性能的木糖异构酶
公开(公告)号：US06475768B1
公开(公告)日：2002-11-05
申请号：US09545569
申请日：2000-04-07
申请人： Ricardo Román Cordero Otero , Márk Gárdonyi , Bärbel Hahn-Hägerdal , Willem Heber van Zyl , Eva Anna Viktoria Dackehag
发明人： Ricardo Román Cordero Otero , Márk Gárdonyi , Bärbel Hahn-Hägerdal , Willem Heber van Zyl , Eva Anna Viktoria Dackehag
IPC分类号： C12N990
CPC分类号： C12Y503/01005 , C12N9/92
摘要： The present invention relates to novel nucleic acid sequences which, upon expression in a procaryotic or eucaryotic cell, code for a polypeptide having an elevated specific xylose isomerase activity compared to the wildtype Thermus thermophilus xylose isomerase. They are selected from a) nucleic acid sequences shown in SEQ. ID. No 1; b) the complementary strand of the sequence defined in (a) above; c) nucleic acid sequences which hybridize to the sequences defined in (a) or (b) above; d) nucleic acid sequences which, but for the degeneracy of the genetic code, would hybridize to the sequences defined in (a), (b) or (c) above and which code for the same polypeptide as those defined in (a), (b) or (c) above. The present invention further provides a process of producing ethanol from xylose containing materials comprising contacting cells that express such nucleic acid sequences and novel modified xylose isomerases that advantageously can be applied in the production of fructose syrups.
摘要翻译：本发明涉及新颖的核酸序列，其在原核或真核细胞中表达时编码与野生型嗜热栖热菌嗜热木糖木糖异构酶相比具有升高的特异性木糖异构酶活性的多肽。它们选自a）SEQ ID NO： ID。没有1; b）上文（a）中定义的序列的互补链; c）与上述（a）或（b）中定义的序列杂交的核酸序列; d）核酸序列，但是对于遗传密码的简并性，其将与上述（a），（b）或（c）中定义的序列杂交，并且与（a）中定义的相同多肽的编码，（b）或（c）。本发明进一步提供了一种从含有木糖的材料生产乙醇的方法，包括使表达这种核酸序列的细胞接触和有利地可用于果糖糖浆生产中的新型修饰的木糖异构酶。

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