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71. 发明授权

US07635578B2 Massive parallel method for decoding DNA and RNA 有权
公开(公告)号：US07635578B2
公开(公告)日：2009-12-22
申请号：US11894808
申请日：2007-08-20
申请人： Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
发明人： Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号： C12P19/34 , C12P1/68 , C07H21/02 , C07H21/00
CPC分类号： C07H19/14 , C07B2200/11 , C07H19/10 , C07H21/00 , C12Q1/68 , C12Q1/686 , C12Q1/6869 , C12Q1/6872 , C12Q1/6874 , C12Q1/6876 , C12Q2525/117 , C12Q2535/101 , C12Q2535/122 , C12Q2563/107 , C40B40/00 , C12Q2565/501 , C12Q2525/186
摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

72. 发明申请

US20090275039A1 METHODS FOR DETECTION AND QUANTIFICATION OF SMALL RNA 有权
标题翻译：小RNA检测和定量方法
公开(公告)号：US20090275039A1
公开(公告)日：2009-11-05
申请号：US12425088
申请日：2009-04-16
申请人： Michael J. Moser , Vecheslav A. Elagin
发明人： Michael J. Moser , Vecheslav A. Elagin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q1/6818 , C12Q2525/207 , C12Q2525/117
摘要： Disclosed are methods, kits, and components for detecting small RNA molecules, such as microRNA and siRNA, in a sample. The methods utilize primers and reporter molecules comprising non-natural bases. The disclosed kits may include one or more components for performing the disclosed methods.
摘要翻译：公开了用于检测样品中的小RNA分子如微RNA和siRNA的方法，试剂盒和组分。该方法利用包含非天然碱基的引物和报道分子。所公开的套件可以包括用于执行所公开的方法的一个或多个组件。

73. 发明授权

US07531312B2 Simplified biological evaluation method of natural and artificial chemicals by using DNA injury index and apparatus therefor 有权
标题翻译：通过使用DNA损伤指数及其设备简化天然和人造化学品的生物学评估方法
公开(公告)号：US07531312B2
公开(公告)日：2009-05-12
申请号：US11311360
申请日：2005-12-20
申请人： Atsushi Takaki
发明人： Atsushi Takaki
IPC分类号： G01N33/53 , C12Q1/68 , C07H11/00
CPC分类号： G01N33/52 , C12Q1/025 , C12Q1/68 , G01N33/5014 , C12Q2525/117 , C12Q2523/113 , C12Q2523/313
摘要： A biological evaluation method for rational and simply evaluating biological harmfulness or usefulness of a great number of natural and artificial chemicals, foods, etc. This method comprises adding a known amount of 2′-deoxyguanosine (dG) to a solution containing a test substance (drug, pesticide, functional food, etc.), optionally applying UV light and/or adding active oxygen generator, then quantifying 8-hydroxy-2′-deoxyguanosine (8OHdG) in the solution, and evaluating the toxicity or usefulness of the test substance according to the 8OhdG content (a higher 8OhdG content indicates a higher harmfulness of the test substance while a lower 8OhdG content indicates a lower harmfulness or an usefulness thereof). The invention also provide an apparatus for advantageously performing said biological evaluation method and an antioxidant preservative solution to be used in this apparatus.
摘要翻译：一种用于合理，简单地评估大量天然和人造化学品，食品等的生物有害性或有用性的生物学评估方法。该方法包括将已知量的2'-脱氧鸟苷（dG）加入到含有测试物质的溶液药物，农药，功能性食品等），任选地应用UV光和/或添加活性氧发生剂，然后定量溶液中的8-羟基-2'-脱氧鸟苷（8OHdG），并评估测试物质的毒性或有用性根据8OhdG含量（较高的8 OhdG含量表示测试物质的较高的有害性，而较低的8 OhdG含量表示较低的有害性或其有用性）。本发明还提供了用于有利地进行所述生物学评价方法的装置和用于该装置中的抗氧化防腐剂溶液。

74. 发明申请

US20090098549A1 SELEX AND PHOTOSELEX 有权
标题翻译： SELEX和PHOTOSELEX
公开(公告)号：US20090098549A1
公开(公告)日：2009-04-16
申请号：US12175388
申请日：2008-07-17
申请人： Daniel J. Schneider , Sheri K. Wilcox , Dominic Zichi , Dan Nieuwlandt , Jeff Carter , Larry Gold
发明人： Daniel J. Schneider , Sheri K. Wilcox , Dominic Zichi , Dan Nieuwlandt , Jeff Carter , Larry Gold
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6811 , C12N15/1048 , C12N15/111 , C12N15/115 , C12N2310/16 , C12N2320/13 , C12Q2541/101 , C12Q2525/205 , C12Q2525/117
摘要： The present disclosure describes improved SELEX methods for generating nucleic acid ligands that are capable of binding to target molecules and improved photoSELEX methods for generating photoreactive nucleic acid ligands that are capable of both binding and covalently crosslinking to target molecules. The disclosure further describes nucleic acid libraries having expanded physical and chemical properties and their use in SELEX and photoSELEX; methods for increasing the crosslinking efficiencies of photoaptamers; methods for producing photoaptamers having selective modifications that enhance functionality and minimize non-specific photoreactions; and methods for generating truncated nucleic acid ligands from nucleic acid ligands of longer length. The disclosure further describes aptamers and photoaptamers obtained by using any of the foregoing.
摘要翻译：本公开描述了用于产生能够结合靶分子的核酸配体的改进的SELEX方法和用于产生能够与靶分子结合和共价交联的光反应性核酸配体的改进的photoSELEX方法。本公开进一步描述了具有扩展的物理和化学性质及其在SELEX和photoSELEX中的用途的核酸文库; 提高光催化剂交联效率的方法; 制备具有增强功能并使非特异性光反应最小化的选择性修饰的光致抗体的方法; 以及用于从较长长度的核酸配体产生截短的核酸配体的方法。本公开进一步描述了通过使用上述任何一种获得的适体和光致抗体。

75. 发明授权

US07517978B1 Modified oligonucleotides and applications thereof 有权
标题翻译：修饰的寡核苷酸及其应用
公开(公告)号：US07517978B1
公开(公告)日：2009-04-14
申请号：US11250192
申请日：2005-10-12
申请人： Khairuzzaman Bashar Mullah , Zhaochun Ma , Wanli Bi
发明人： Khairuzzaman Bashar Mullah , Zhaochun Ma , Wanli Bi
IPC分类号： C07H21/04 , C07H21/02 , C12Q1/68 , C12P19/34
CPC分类号： C07H21/04 , C07H21/02 , C12Q1/6848 , C12Q2525/117
摘要： Disclosed, among other things, are primers containing certain modified nucleobases in the 3′ terminal region of the primers that provide reduced formation of primer-dimers during amplification reactions, and various methods of use thereof.
摘要翻译：公开了其中包含在引物的3'末端区域中含有某些修饰的核碱基的引物，其在扩增反应期间提供缩短的引物二聚体的形成及其各种使用方法。

76. 发明申请

US20090064377A1 METHOD AND MEANS FOR TARGETED NUCLEOTIDE EXCHANGE 审中-公开
标题翻译：靶向核交换的方法和手段
公开(公告)号：US20090064377A1
公开(公告)日：2009-03-05
申请号：US12088813
申请日：2005-09-29
申请人： Daphne Yvette Rainey-Wittich , Michiel Theodoor Jan de Both , Paul Bundock
发明人： Daphne Yvette Rainey-Wittich , Michiel Theodoor Jan de Both , Paul Bundock
IPC分类号： A01H5/00 , C07H21/04 , C12N9/99 , C12N9/00 , C12N5/10
CPC分类号： C12N15/102 , C12Q1/6827 , C12Q2537/1373 , C12Q2537/107 , C12Q2525/117 , C12Q2523/115
摘要： The invention pertains to a method for targeted alteration of a duplex acceptor DNA sequence, comprising combining the duplex acceptor DNA sequence with a donor oligonucleotide, wherein the duplex acceptor DNA sequence contains a first DNA sequence and a second DNA sequence which is the complement of the first DNA sequence and wherein the donor oligonucleotide comprises a domain that comprises at least one mismatch with respect to the duplex acceptor DNA sequence to be altered, preferably with respect to the first DNA sequence, and wherein a section of the donor oligonucleotide is methylated to a higher degree than the second DNA sequence and/or wherein the second DNA is methylated to a lower degree than the corresponding section of the donor oligonucleotide, optionally in the presence of proteins that are capable of targeted nucleotide exchange and to oligonucleotides for use in the method.
摘要翻译：本发明涉及双重受体DNA序列的靶向改变的方法，包括将双链体受体DNA序列与供体寡核苷酸组合，其中双链体受体DNA序列含有第一DNA序列，第二DNA序列是作为第一DNA序列，并且其中所述供体寡核苷酸包含相对于待改变的双链体受体DNA序列至少一个错配的结构域，优选相对于第一DNA序列，并且其中将所述供体寡核苷酸的一部分甲基化为比第二DNA序列更高的程度和/或其中第二DNA被甲基化至低于供体寡核苷酸的相应部分的程度，任选地在能够靶向核苷酸交换的蛋白质存在下和用于该方法的寡核苷酸。

77. 发明申请

US20090042732A1 METHODS FOR SIMPLIFYING MICROBIAL NUCLEIC ACIDS BY CHEMICAL MODIFICATION OF CYTOSINES 有权
标题翻译：通过化学修饰细胞因子来简化微生物核酸的方法
公开(公告)号：US20090042732A1
公开(公告)日：2009-02-12
申请号：US11573873
申请日：2005-12-05
申请人： Douglas Spencer Millar , George L Gabor Miklos
发明人： Douglas Spencer Millar , George L Gabor Miklos
IPC分类号： C40B30/02 , C07H21/00 , C12Q1/68 , C12Q1/70 , C40B30/04
CPC分类号： C12Q1/6876 , C12Q1/6806 , C12Q1/689 , C12Q2600/16 , C12Q2523/125 , C12Q2525/117
摘要： A method for simplification of a microbial genome or microbial nucleic acid comprising treating microbial genome or nucleic acid with an agent that modifies cytosine to form derivative microbial nucleic acid and amplifying the derivative microbial nucleic acid to produce a simplified form of the microbial genome or nucleic acid.
摘要翻译：一种用于简化微生物基因组或微生物核酸的方法，其包括用修饰胞嘧啶形成衍生的微生物核酸并扩增衍生的微生物核酸以产生微生物基因组或核酸的简化形式的试剂处理微生物基因组或核酸。

78. 发明申请

US20090005334A1 Nucleic acid derivatives 有权
标题翻译：核酸衍生物
公开(公告)号：US20090005334A1
公开(公告)日：2009-01-01
申请号：US12071275
申请日：2008-02-19
申请人： David Segev
发明人： David Segev
IPC分类号： A61K48/00 , C12N7/06 , C07H21/04 , A61K31/675 , A61K31/52 , A61K31/506 , C07D473/00 , C07D403/00 , C12N5/00 , C12N1/00 , C12N5/04
CPC分类号： C12Q1/6839 , C07B2200/11 , C12Q1/6832 , C12Q2525/117 , C12Q2525/113
摘要： A compound which comprises a backbone having a plurality of chiral carbon atoms, the backbone bearing a plurality of ligands each being individually bound to a chiral carbon atom of the plurality of chiral carbon atoms, the ligands including one or more pair(s) of adjacent ligands each containing a moiety selected from the group consisting of a naturally occurring nucleobase and a nucleobase binding group, wherein moieties of the one or more pair(s) are directly linked to one another via a linker chain; building blocks for synthesizing the compound; and uses of the compound, particularly in antisense therapy.
摘要翻译：包含具有多个手性碳原子的骨架的化合物，所述骨架具有多个配体，每个配体各自与多个手性碳原子的手性碳原子结合，所述配体包括一个或多个相邻的一对各自含有选自天然存在的核碱基和核碱基结合基团的部分的配体，其中所述一个或多个对的部分通过连接体链彼此直接连接; 用于合成化合物的结构单元; 和化合物的用途，特别是在反义治疗中。

79. 发明申请

US20080199868A1 Massive parallel method for decoding DNA and RNA 有权
标题翻译：用于解码DNA和RNA的大规模并行方法
公开(公告)号：US20080199868A1
公开(公告)日：2008-08-21
申请号：US11894808
申请日：2007-08-20
申请人： Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
发明人： Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C07H19/14 , C07B2200/11 , C07H19/10 , C07H21/00 , C12Q1/68 , C12Q1/686 , C12Q1/6869 , C12Q1/6872 , C12Q1/6874 , C12Q1/6876 , C12Q2525/117 , C12Q2535/101 , C12Q2535/122 , C12Q2563/107 , C40B40/00 , C12Q2565/501 , C12Q2525/186
摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
摘要翻译：本发明提供了通过在聚合酶反应中将核苷酸类似物掺入DNA的生长链中之后检测核苷酸类似物的同一性来提供将核酸连接到固体表面和测序核酸的方法。本发明还提供核苷酸类似物，其包含通过可切割连接子连接至核苷酸类似物的独特标记，以及可脱氧化学基团以封端脱氧核糖3'-位上的-OH基团。

80. 发明授权

US07405281B2 Fluorescent nucleotide analogs and uses therefor 有权
标题翻译：荧光核苷酸类似物及其用途
公开(公告)号：US07405281B2
公开(公告)日：2008-07-29
申请号：US11241809
申请日：2005-09-29
申请人： Yue Xu , Jeffrey Wegener , Arek Bibillo
发明人： Yue Xu , Jeffrey Wegener , Arek Bibillo
IPC分类号： C07G3/00 , C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C07H19/10 , C07H21/00 , C12Q1/6869 , C12Q2533/101 , C12Q2525/117 , C12Q2525/101
摘要： Labeled nucleotide analogs used in place of naturally occurring nucleoside triphosphates or other analogs in template directed nucleic acid synthesis reactions and other nucleic acid reactions, and various analyses based thereon, including DNA sequencing, single base identification, hybridization assays and others.
摘要翻译：在模板定向核酸合成反应和其他核酸反应中使用代替天然存在的核苷三磷酸或其他类似物的标记的核苷酸类似物，以及基于其的各种分析，包括DNA测序，单碱基鉴定，杂交测定等。

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