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1. 发明授权

US08343738B2 Assay for screening for potential cervical cancer 有权
标题翻译：检测潜在宫颈癌的检测
公开(公告)号：US08343738B2
公开(公告)日：2013-01-01
申请号：US12066644
申请日：2006-09-14
申请人： Douglas Spencer Millar , John R. Melki , George L. Gabor Miklos
发明人： Douglas Spencer Millar , John R. Melki , George L. Gabor Miklos
IPC分类号： C12P19/34
CPC分类号： C12Q1/6883 , C12Q2600/154 , C12Q2600/16
摘要： The invention relates to an assay for determining a health state of a subject using a combination of detecting the presence of a virus and detecting the presence of a genomic target or marker indicative of a health state.
摘要翻译：本发明涉及使用检测病毒存在和检测指示健康状态的基因组靶标或标志物的存在的组合来确定受试者的健康状态的测定法。

2. 发明授权

US08598088B2 Methods for simplifying microbial nucleic acids by chemical modification of cytosines 有权
标题翻译：通过化学修饰胞嘧啶简化微生物核酸的方法
公开(公告)号：US08598088B2
公开(公告)日：2013-12-03
申请号：US12892484
申请日：2010-09-28
申请人： Douglas Spencer Millar , George L. Gabor Miklos
发明人： Douglas Spencer Millar , George L. Gabor Miklos
IPC分类号： C12Q1/70
CPC分类号： C12Q1/6876 , C12Q1/6806 , C12Q1/689 , C12Q2600/16 , C12Q2523/125 , C12Q2525/117
摘要： A method for detecting a microorganism comprising reducing the complexity of a microbial genome or microbial nucleic acid by generating a simplified form of the microbial genome or microbial nucleic acid in which substantially all of the positions naturally occupied by cytosines are occupied by uracil or thymine; and assaying for a microbial specific nucleic acid in the simplified form of the microbial genome or microbial nucleic acid, wherein presence of the microbial specific nucleic acid is indicative of the microorganism.
摘要翻译：一种用于检测微生物的方法，包括通过产生微生物基因组或微生物核酸的简化形式降低微生物基因组或微生物核酸的复杂性，其中基本上所有由胞嘧啶所占据的位置都被尿嘧啶或胸腺嘧啶占据; 并以微生物基因组或微生物核酸的简化形式测定微生物特异性核酸，其中微生物特异性核酸的存在指示微生物。

3. 发明申请

US20090042732A1 METHODS FOR SIMPLIFYING MICROBIAL NUCLEIC ACIDS BY CHEMICAL MODIFICATION OF CYTOSINES 有权
标题翻译：通过化学修饰细胞因子来简化微生物核酸的方法
公开(公告)号：US20090042732A1
公开(公告)日：2009-02-12
申请号：US11573873
申请日：2005-12-05
申请人： Douglas Spencer Millar , George L Gabor Miklos
发明人： Douglas Spencer Millar , George L Gabor Miklos
IPC分类号： C40B30/02 , C07H21/00 , C12Q1/68 , C12Q1/70 , C40B30/04
CPC分类号： C12Q1/6876 , C12Q1/6806 , C12Q1/689 , C12Q2600/16 , C12Q2523/125 , C12Q2525/117
摘要： A method for simplification of a microbial genome or microbial nucleic acid comprising treating microbial genome or nucleic acid with an agent that modifies cytosine to form derivative microbial nucleic acid and amplifying the derivative microbial nucleic acid to produce a simplified form of the microbial genome or nucleic acid.
摘要翻译：一种用于简化微生物基因组或微生物核酸的方法，其包括用修饰胞嘧啶形成衍生的微生物核酸并扩增衍生的微生物核酸以产生微生物基因组或核酸的简化形式的试剂处理微生物基因组或核酸。

4. 发明授权

US07846693B2 Nucleic acid detection assay 有权
标题翻译：核酸检测法
公开(公告)号：US07846693B2
公开(公告)日：2010-12-07
申请号：US10570715
申请日：2004-09-03
申请人： Douglas Spencer Millar , John Robert Melki , George L. Gabor Miklos
发明人： Douglas Spencer Millar , John Robert Melki , George L. Gabor Miklos
IPC分类号： C12P19/34
CPC分类号： C12Q1/6827 , C12Q2533/107 , C12Q2525/307 , C12Q2523/125 , C12Q2521/325
摘要： A method for determining the methylation status of a potential methylation site in genomic nucleic acid comprising treating genomic nucleic acid with an agent which modifies cytosine bases but does not modify 5methyl-cytosine bases under conditions to form a. modified nucleic acid template containing a potential methylation site; providing a first clamp containing a first capture sequence complementary to a region flanking one side of the potential methylation site in the modified nucleic acid template, providing a second clamp containing a second capture sequence complementary to a region flanking the other side of the potential methylation site in the modified nucleic acid template; allowing the first clamp and the second clamp to hybridise to the modified nucleic acid template; ligating the hybridised first and second clamps to form a probe spanning the potential methylation site in the modified nucleic acid template; digesting the modified nucleic acid template to obtain the probe; and detecting the probe and determining the methylation status of the potential methylation site in the modified genomic nucleic acid.
摘要翻译：一种确定基因组核酸中潜在的甲基化位点的甲基化状态的方法，其包括用修饰胞嘧啶碱基但不改变5个甲基胞嘧啶碱基的试剂处理基因组核酸。含有潜在甲基化位点的修饰的核酸模板; 提供第一夹具，其含有与修饰的核酸模板中的潜在甲基化位点侧翼侧的区域互补的第一捕获序列，提供包含与潜在甲基化位点的另一侧侧翼区域互补的第二捕获序列的第二夹在修饰的核酸模板中; 允许第一夹具和第二夹钳与修饰的核酸模板杂交; 连接杂交的第一和第二夹具以形成跨越修饰的核酸模板中的潜在甲基化位点的探针; 消化修饰的核酸模板以获得探针; 并检测探针并测定修饰基因组核酸中潜在甲基化位点的甲基化状态。

5. 发明授权

US07833942B2 Methods for simplifying microbial nucleic acids by chemical modification of cytosines 有权
标题翻译：通过化学修饰胞嘧啶来简化微生物核酸的方法
公开(公告)号：US07833942B2
公开(公告)日：2010-11-16
申请号：US11573873
申请日：2005-12-05
申请人： Douglas Spencer Millar , George L. Gabor Miklos
发明人： Douglas Spencer Millar , George L. Gabor Miklos
IPC分类号： C40B30/02 , C40B30/04 , C07H21/00 , C12Q1/68 , C12Q1/70 , A61K31/00
CPC分类号： C12Q1/6876 , C12Q1/6806 , C12Q1/689 , C12Q2600/16 , C12Q2523/125 , C12Q2525/117
摘要： A method for simplification of a microbial genome or microbial nucleic acid comprising treating microbial genome or nucleic acid with an agent that modifies cytosine to form derivative microbial nucleic acid and amplifying the derivative microbial nucleic acid to produce a simplified form of the microbial genome or nucleic acid.
摘要翻译：一种用于简化微生物基因组或微生物核酸的方法，其包括用修饰胞嘧啶形成衍生的微生物核酸并扩增衍生的微生物核酸以产生微生物基因组或核酸的简化形式的试剂处理微生物基因组或核酸。

6. 发明申请

US20150086972A1 MOLECULAR DETECTION ASSAY 有权
标题翻译：分子检测测定
公开(公告)号：US20150086972A1
公开(公告)日：2015-03-26
申请号：US14342093
申请日：2011-09-07
申请人： Douglas Spencer Millar
发明人： Douglas Spencer Millar
IPC分类号： C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/6806 , C12Q1/6886 , C12Q1/689 , C12Q1/6893 , C12Q1/707 , C12Q2600/158 , C12Q2523/125
摘要： A molecular detection assay including treating a biological sample directly with a bisulphite agent under conditions that allow cell disruption and nucleic acid treatment; removing the bisulphite agent from the treated sample; and detecting a target nucleic acid in the treated sample.
摘要翻译：一种分子检测测定法，包括在允许细胞破碎和核酸处理的条件下用亚硫酸氢盐剂直接处理生物样品; 从处理过的样品中除去亚硫酸氢盐; 并检测被处理样品中的靶核酸。

7. 发明申请

US20110003700A1 ELIMINATION OF CONTAMINANTS ASSOCIATED WITH NUCLEIC ACID AMPLIFICATION 审中-公开
标题翻译：消除与核酸放大相关的污染物
公开(公告)号：US20110003700A1
公开(公告)日：2011-01-06
申请号：US12747483
申请日：2008-12-19
申请人： Douglas Spencer Millar
发明人： Douglas Spencer Millar
IPC分类号： C40B30/00 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6848 , C12Q2525/101 , C12Q2521/301 , C12Q2525/117
摘要： Use of a non-natural base with an enzyme capable of degrading a nucleic acid containing a non-natural base in an amplification reaction to eliminate carry-over contaminants in the amplification reaction.
摘要翻译：在扩增反应中使用非天然碱与能够在扩增反应中降解含有非天然碱基的核酸的酶以消除扩增反应中的携带污染物。

8. 发明申请

US20090263909A1 TREATMENT OF NUCLEIC ACID 有权
标题翻译：治疗核酸
公开(公告)号：US20090263909A1
公开(公告)日：2009-10-22
申请号：US12413380
申请日：2009-03-27
申请人： Douglas Spencer Millar , Cassandra Jean Vockler , Neralie Ann Coulston
发明人： Douglas Spencer Millar , Cassandra Jean Vockler , Neralie Ann Coulston
IPC分类号： G01N33/00 , C07H1/00
CPC分类号： C12Q1/6827 , Y10T436/143333 , C12Q2527/119 , C12Q2523/125
摘要： Methods for treating nucleic acid including: (a) providing an alkali environment to a nucleic acid sample; (b) reacting the nucleic acid sample with a bisulphite reagent and incubating the reaction so as to form a treated nucleic acid sample where methylated nucleotides in the nucleic acid sample remain unchanged while unmethylated nucleotides are converted to another form; (c) removing unwanted reagents or diluents from the treated nucleic acid sample; and (d) carrying out de-sulphonation of the precipitated treated nucleic acid at a temperature from 70° C. to 95° C. by adjusting the precipitated treated nucleic acid to a pH of between 10 and less than 12.5 to remove sulphonate groups present on the treated nucleic acid and obtain a nucleic acid sample substantially free of sulphonate groups.
摘要翻译：用于处理核酸的方法包括：（a）向核酸样品提供碱性环境; （b）使核酸样品与亚硫酸氢盐试剂反应并孵育反应以形成处理的核酸样品，其中核酸样品中的甲基化核苷酸保持不变，而未甲基化核苷酸转化为另一种形式; （c）从经处理的核酸样品中除去不想要的试剂或稀释剂; 和（d）通过将沉淀的经处理的核酸调节至10至小于12.5的pH来进行沉淀处理的核酸在70℃至95℃的温度下的脱磺化，以除去存在的磺酸盐基团在经处理的核酸上获得基本上不含磺酸盐基团的核酸样品。

9. 发明授权

US08685675B2 Enzymes for amplification and copying bisulphite modified nucleic acids 有权
标题翻译：用于扩增和复制亚硫酸氢盐修饰的核酸的酶
公开(公告)号：US08685675B2
公开(公告)日：2014-04-01
申请号：US12744305
申请日：2008-11-27
申请人： Douglas Spencer Millar
发明人： Douglas Spencer Millar
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12P19/34 , C12N9/1252 , C12Q1/6848 , C12Q1/6858 , C12Q2523/125 , C12Q2521/101
摘要： The invention relates to the use of enzymes for copying or amplifying bisulphite modified or treated nucleic acids, wherein the enzymes are more effective in copying or amplifying the nucleic acid compared with native Taq polymerase under substantially the same conditions.
摘要翻译：本发明涉及用于复制或扩增亚硫酸氢盐修饰或处理的核酸的酶的用途，其中所述酶在基本相同的条件下与天然Taq聚合酶相比更有效地复制或扩增核酸。

10. 发明授权

US08431347B2 Isothermal strand displacement amplification using primers containing a non-regular base 有权
标题翻译：使用含有非规则碱基的引物进行等温链置换扩增
公开(公告)号：US08431347B2
公开(公告)日：2013-04-30
申请号：US11919443
申请日：2006-05-25
申请人： Douglas Spencer Millar , John R. Melki , Ailsa Grigg
发明人： Douglas Spencer Millar , John R. Melki , Geoffrey W. Grigg
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6865 , C12Q2525/117 , C12Q2523/125 , C12Q2521/301
摘要： The invention is directed to a method for isothermal DNA amplification comprising providing to the DNA to be amplified an amplification mix comprising a first primer at least partially complementary to a region of DNA and containing a non-regular base, a second primer at least partially complementary to a region of DNA and containing a non- regular base, a DNA polymerase, an enzyme capable of strand displacement, an enzyme that recognises a non-regular base in double-stranded DNA and causes a nick or excises a base in one DNA strand at or near the non-regular base; and amplifying the DNA substantially without thermal cycling.
摘要翻译：本发明涉及一种用于等温DNA扩增的方法，其包括提供待扩增的DNA扩增混合物，所述扩增混合物包含至少部分互补于DNA区域并含有非正常碱基的第一引物，至少部分互补的第二引物到DNA的区域并含有非规则碱基，DNA聚合酶，能够置换链的酶，识别双链DNA中的非规则碱基并在一个DNA链中产生切口或切除碱基的酶在非正规基地或附近; 并且基本上没有热循环扩增DNA。

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