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71. 发明申请

US20180127732A1 FUSION POLYMERASES 审中-公开
公开(公告)号：US20180127732A1
公开(公告)日：2018-05-10
申请号：US15801114
申请日：2017-11-01
申请人： Bio-Rad Laboratories, Inc.
发明人： Yan WANG , Man CHENG
IPC分类号： C12N9/12 , C12Q1/686 , C12N9/22
CPC分类号： C12N9/1252 , C07K2319/80 , C12N9/22 , C12Q1/686
摘要： Fusion polypeptides having a heterologous 5′-3′ exonuclease domain linked to a polymerase that does not naturally have 5′-3′ exonuclease activity, as well as methods of their use are provided. Other aspects are also disclosed.

72. 发明申请

US20180126383A1 MICROORGANISM NUCLEIC ACID PURIFICATION FROM HOST SAMPLES 审中-公开
公开(公告)号：US20180126383A1
公开(公告)日：2018-05-10
申请号：US15859805
申请日：2018-01-02
申请人： Abbott Molecular Inc.
发明人： Herbert A. Marble , Thomas Laffler
IPC分类号： B01L7/00 , C07H21/00 , C12P19/34 , B01L3/00
CPC分类号： B01L7/54 , B01L3/502784 , B01L7/525 , B01L2400/0415 , B01L2400/0433 , B01L2400/0445 , B01L2400/086 , C07H21/00 , C12P19/34 , C12Q1/686 , C12Q2527/15 , C12Q2563/159 , C12Q2565/629
摘要： The present disclosure provides systems, devices, and methods for purifying microorganism nucleic acid from a host sample, such as a whole blood sample from a human. In certain embodiments, devices and systems with multiple filters are employed and provide for the selective removal of blood cells and host nucleic acids from a sample in order to enrich for microorganism nucleic acid.

73. 发明申请

US20180119116A1 DNA POLYMERASES WITH INCREASED 3'-MISMATCH DISCRIMINATION 审中-公开
公开(公告)号：US20180119116A1
公开(公告)日：2018-05-03
申请号：US15804489
申请日：2017-11-06
申请人： Roche Molecular Systems, Inc.
发明人： Fred REICHERT , Keith BAUER , Thomas W. MYERS
IPC分类号： C12N9/12 , C12P19/34 , C12Q1/6827 , C12Q1/6848 , C12Q1/6858 , C12Q1/686
CPC分类号： C12N9/1252 , C12P19/34 , C12Q1/6827 , C12Q1/6848 , C12Q1/6858 , C12Q1/686 , C12Y207/07007 , C12Q2521/101 , C12Q2535/125
摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

74. 发明授权

US09957559B2 Solid gel amplification method and apparatus for platform molecular diagnostics 有权
公开(公告)号：US09957559B2
公开(公告)日：2018-05-01
申请号：US14399637
申请日：2013-02-25
申请人： Damikka Manage
发明人： Damikka Manage
IPC分类号： C12Q1/68 , B01L7/00 , B01L3/00 , B01L9/06 , C12M1/40 , C12M3/06 , C12M1/00 , C12P19/34
CPC分类号： C12Q1/686 , B01L3/502707 , B01L3/50851 , B01L7/52 , B01L9/065 , B01L2200/0673 , B01L2200/0689 , B01L2300/069 , B01L2300/0816 , B01L2300/0838 , B01L2300/12 , C12M21/18 , C12M23/16 , C12M23/20 , C12M43/00 , C12P19/34
摘要： The present invention provides for a novel system and method for amplification and detection of nucleic acids within a miniaturized device wherein sample administration occurs via capillary forces through a channel created by drying a hydrogel containing all components needed for a cell-free, enzymatic, nucleic-acid amplification system other than the template nucleic acid or precursor thereto, and wherein an aqueous sample is provided to the desiccated hydrogel, and the hydrogel is rehydrated, through capillary forces.

75. 发明授权

US09957558B2 Methods and compositions for multiplex PCR 有权
公开(公告)号：US09957558B2
公开(公告)日：2018-05-01
申请号：US13458739
申请日：2012-04-27
申请人： John Leamon , Mark Andersen , Michael Thornton
发明人： John Leamon , Mark Andersen , Michael Thornton
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12Q1/686 , C12N15/1065 , C12Q1/6806 , C12Q1/6855 , C12Q1/6874 , C12Q1/6886 , C12Q2600/16 , C12Q2521/307 , C12Q2525/101 , C12Q2525/191 , C12Q2537/143
摘要： The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

76. 发明申请

US20180113137A1 METHOD FOR DETECTING THE QUANTITY OF BIOMARKER AND IDENTIFYING DISEASE STATUS 审中-公开
公开(公告)号：US20180113137A1
公开(公告)日：2018-04-26
申请号：US15821864
申请日：2017-11-24
申请人： TCM BIOTECH INTERNATIONAL CORP.
发明人： PEI-JER CHEN , SHIOU-HWEI YEH , CHIAO-LING LI , DING-SHINN CHEN , YA-CHUN WANG , SHENG-TAI TZENG
IPC分类号： G01N33/574 , C12Q1/6886 , C12Q1/6806 , C12Q1/6809 , C12Q1/686 , G01N33/576
CPC分类号： G01N33/57488 , C12Q1/6806 , C12Q1/6809 , C12Q1/686 , C12Q1/6886 , C12Q2600/106 , C12Q2600/112 , C12Q2600/118 , C12Q2600/158 , C12Q2600/16 , G01N33/5761 , G01N2800/085 , G01N2800/52 , G01N2800/56 , G01N2800/7028
摘要： The present invention provides a chimera nucleic acid obtained from circulatory system for monitoring tumor size. The nucleic acid comprises partial sequence derived from host genome and partial sequence derived from non-host genome. The partial sequence derived from host genome and the partial sequence derived from non-host genome form a chimera junction. The chimera junction is obtained from cell-free nucleic acids and is indicative of tumor size.

77. 发明申请

US20180113133A1 GENE RELEVANT TO PAPILLARY THYROID TUMORS 审中-公开
公开(公告)号：US20180113133A1
公开(公告)日：2018-04-26
申请号：US15810383
申请日：2017-11-13
申请人： GUANGZHOU HEERSHI PHARMA DEVELOPMENT CO., LTD
发明人： LIANGZHONG XIONG
IPC分类号： G01N33/574 , C12Q1/686 , A61K48/00 , A61P35/00
CPC分类号： G01N33/57407 , A61K48/00 , A61K48/005 , A61P35/00 , C12Q1/686 , C12Q1/6886 , C12Q2600/158
摘要： The invention relates to a gene relevant to papillary thyroid tumors and an application thereof. According to the base sequence of the gene, real-time and quantitative PCR (Polymerase Chain Reaction) primers are designed and synthesized; the expression level of long-chain non-coding RNA (Ribonucleic Acid) transcribed by the gene is detected in a papillary thyroid carcinoma clinical case specimen; the result shows remarkable reducing of the expression level of the long-chain non-coding RNA in papillary thyroid tumor tissues and the long-chain non-coding RNA of the gene silencing can remarkably promote the growth of thyroid cancer cells. The gene relevant to the papillary thyroid tumors is expected to prepare preparations used in papillary thyroid carcinoma auxiliary diagnosis, gene therapy, curative effect prediction or prognosis.

78. 发明授权

US09944993B2 Method for enrichment of circulating tumor DNA and reagent for enrichment of circulating tumor DNA 有权
公开(公告)号：US09944993B2
公开(公告)日：2018-04-17
申请号：US15127736
申请日：2015-01-06
申请人： HAPLOX BIOTECHNOLOGY(SHENZHEN) CO., LTD.
发明人： Mingyan Xu , Xiaoni Zhang
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6806 , C12Q1/686 , C12Q2600/158 , C12Q2531/113 , C12Q2563/159 , C12Q2565/119
摘要： The present invention provides a method and a reagent for enrichment of circulating tumor DNA, the method comprising the steps of mixing a water phase and an oil phase and shaking the mixture to prepare an emulsion PCR reaction system, and performing emulsion PCR amplification, wherein the water phase comprises peripheral blood plasma DNA as template DNA, a forward primer and a reverse primer, dNTPs, a PCR buffer and a DNA polymerase, the peripheral blood plasma DNA having adapter sequences connected to both ends thereof, and the forward primer and the reverse primer being complementary to the adapter sequences at the two ends respectively; separating the water phase from the oil phase following the emulsion PCR amplification to obtain a PCR amplification product in the water phase; and capturing circulating tumor DNA in the PCR amplification product in the water phase by using a probe sequence that specifically binds to the circulating tumor DNA. The method of the present invention is capable of performing single-molecule high-fidelity ultramicro parallel amplification and effectively capturing peripheral blood plasma ctDNA to provide adequate amount of ctDNA to be used for subsequent sequencing detection.

79. 发明授权

US09938581B2 Primers for diagnosing Avellino corneal dystrophy 有权
公开(公告)号：US09938581B2
公开(公告)日：2018-04-10
申请号：US14454669
申请日：2014-08-07
申请人： Avellino Co., Ltd.
发明人： Gene Lee , Jung Kuk Yun
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6883 , C12Q1/686 , C12Q2600/112 , C12Q2600/156 , C12Q2561/113
摘要： The present invention relates to a real-time PCR primer pair and probe for diagnosing Avellino corneal dystrophy, and more particularly to a real-time PCR primer pair and probe for diagnosing Avellino corneal dystrophy, which can accurately diagnose the presence or absence of a mutation in exon 4 of BIGH3 gene, which is responsible for Avellino corneal dystrophy. The use of the primer pair and probe according to the invention can diagnose Avellino corneal dystrophy in a more rapid and accurate manner than a conventional method that uses a DNA chip or PCR.

80. 发明授权

US09938570B2 Methods and compositions for universal detection of nucleic acids 有权
公开(公告)号：US09938570B2
公开(公告)日：2018-04-10
申请号：US14566596
申请日：2014-12-10
申请人： UniTaq Bio
发明人： Eugene Spier
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12N2310/141 , C12Q1/686 , C12Q2565/107 , C12Q2525/301 , C12Q2525/155 , C12Q2565/1015
摘要： Provided are methods and compositions for detecting the presence or amount of one or more target nucleic acids in a sample. Methods of the present invention include linking universal nucleic acid segments into a single molecule in a linking reaction dependent on a target nucleic acid of interest. A variety of universal segment linking strategies are provided, including preamplification by polymerase chain reaction, ligation-based strategies, reverse transcription and linear polymerase extension. Linking the universal segments into a single molecule generates a tagged target nucleic acid which is detected in a manner dependent on an intramolecular interaction between one universal segment and a second portion of the tagged target nucleic acid. In certain embodiments, the intramolecular interaction includes the formation of a hairpin having a stem between a universal segment at one end of the tagged target nucleic acid and a second universal segment at the opposite end of the tagged target nucleic acid. A variety of detection formats are provided, including solution-phase and surface-based formats. The methods and compositions are well-suited for highly multiplexed nucleic acid detection, and are applicable for the detection of any target nucleic acid of interest in both research and clinical settings.

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