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61. 发明授权

US09322013B2 Magnetic separation of algae 有权
标题翻译：藻类的磁分离
公开(公告)号：US09322013B2
公开(公告)日：2016-04-26
申请号：US14189969
申请日：2014-02-25
申请人： Los Alamos National Security, LLC
发明人： Pulak Nath , Scott N. Twary
IPC分类号： C12N13/00 , C12N1/02 , C12N1/12
CPC分类号： C12N13/00 , C12N1/02 , C12N1/12
摘要： Described herein are methods and systems for harvesting, collecting, separating and/or dewatering algae using iron based salts combined with a magnetic field gradient to separate algae from an aqueous solution.
摘要翻译：本文描述了使用铁基盐与磁场梯度结合来收集，收集，分离和/或脱水藻类的方法和系统，以将藻类与水溶液分离。

62. 发明授权

US09222067B2 Single use centrifuge system for highly concentrated and/or turbid feeds 有权
标题翻译：用于高度浓缩和/或混浊进料的单次离心机系统
公开(公告)号：US09222067B2
公开(公告)日：2015-12-29
申请号：US13684051
申请日：2012-11-21
申请人： Pneumatic Scale Corporation
发明人： Stephen B Kessler , T. David Marro
IPC分类号： C12M1/00 , C12N1/02 , B04B5/04
CPC分类号： C12M47/02 , B04B5/0442 , B04B2005/0464 , C12M23/26 , C12M23/28 , C12M27/10 , C12M29/00 , C12M37/04 , C12N1/02
摘要： A method and apparatus for cell harvest of production scale quantities of cell cultures using single use components comprising a flexible membrane mounted on a rigid frame and is supported within a multiple use rigid centrifuge bowl, such single use components including a core with an increased diameter and an internal truncated cone shape in order to permit the system to maintain a sufficiently high angular velocity to create a settling velocity suited to efficiently processing highly concentrated cell culture streams. Features which minimize feed turbidity, and others which permit the continuous or semi-continuous discharge of cell concentrate, increase the overall production rate over the rate which can be achieved using current intermittent processing methods for large cell culture volumes. Injection of a diluent during the cell concentrate removal process permits more complete removal of viscous cell concentrates.
摘要翻译：一种用于使用单一使用部件的生产规模量的细胞培养物细胞收获的方法和装置，其包括安装在刚性框架上的柔性膜，并且被支撑在多用刚性离心机碗内，所述单一使用部件包括直径增加的芯和内部截锥形，以便允许系统保持足够高的角速度以产生适于有效处理高度浓缩的细胞培养物流的沉降速度。使进料浊度最小化的特征以及允许连续或半连续排出细胞浓缩物的其它特性使总生产率提高了超过使用目前间歇式处理大量细胞培养物体积的速率。在细胞浓缩物移除过程期间注射稀释剂允许更完全地去除粘性细胞浓缩物。

63. 发明授权

US08940531B2 System for culturing and recovering micro algae 有权
标题翻译：微藻培养和回收系统
公开(公告)号：US08940531B2
公开(公告)日：2015-01-27
申请号：US13300893
申请日：2011-11-21
申请人： Kwang Mo Seong
发明人： Kwang Mo Seong
IPC分类号： C12M1/42 , C12M1/00 , C12N1/12 , C12N1/02 , C12M1/26
CPC分类号： C12M47/02 , C12M21/02 , C12M23/58 , C12M29/02 , C12M33/22 , C12N1/02 , C12N1/12
摘要： A system for culturing and recovering micro algae comprises a photo-bioreactor, a floatation separator, a centrifugal separator, and a micro bubble generator. The photo-bioreactor unit is configured to culture micro algae by a photochemical reaction to produce a micro algae precipitate. The precipitated micro algae is separated by the floatation separator. The separated micro algae is concentrated by the centrifugal separator. The micro bubble generator generates process water containing micro carbon dioxide bubbles and supplies the generated process water to the photo-bioreactor unit and the floatation separator. With this system, micro algae can be cultured and recovered in a simpler and more cost-effective manner.
摘要翻译：用于培养和回收微藻的系统包括光生物反应器，浮选分离器，离心分离器和微气泡发生器。光生物反应器单元被配置为通过光化学反应培养微藻以产生微藻沉淀。沉淀的微藻被浮选分离器分离。分离的微藻被离心分离器浓缩。微气泡发生器产生含有微量二氧化碳气泡的工艺水，并将生成的工艺水供应到光生物反应器单元和浮选分离器。利用该系统，可以以更简单和更具成本效益的方式培养和回收微藻。

64. 发明授权

US08906229B2 Device for a biological liquid treatment installation 有权
标题翻译：生物液体处理装置
公开(公告)号：US08906229B2
公开(公告)日：2014-12-09
申请号：US13116508
申请日：2011-05-26
申请人： Sebastien Cirou , Rene Reinbigler , Virginie Buisson , Jean-Louis Weissenbach
发明人： Sebastien Cirou , Rene Reinbigler , Virginie Buisson , Jean-Louis Weissenbach
IPC分类号： B01D65/00 , B01D15/08 , A61B9/00 , B01D15/10 , C12M1/00 , C12M1/12 , C12N1/02 , C12N1/34 , B01D61/18 , B01D61/20 , F04B43/08
CPC分类号： F17D1/00 , B01D61/18 , B01D61/20 , B01D61/30 , B01D2313/105 , B01D2313/125 , B01D2313/58 , B30B7/00 , B30B9/22 , C12M47/12 , F04B43/08 , Y10T137/0318 , Y10T137/8593
摘要： The invention concerns a device comprising: a base (2); a moveable or removable door (20), said device having a closed door position; and in the closed door position, a circuit (8) comprising a bag comprising two flexible films and conveying network connectors, and a press (9) comprising a first shell (16) disposed on said front face (5) of said base (2) and a second shell (17) disposed in said door (20); said bag being clamped between said first shell (16) and said second shell (17) in a state in which conduits of said network for conveying liquid are formed between said films.
摘要翻译：本发明涉及一种装置，包括：基座（2）; 一个可移动或可拆卸的门（20），所述装置具有一个闭合的门位置; 并且在关闭的门位置，包括包括两个柔性膜和传送网络连接器的袋的电路（8）和包括设置在所述基座（2）的所述前表面（5）上的第一壳体（16）的压力机（9））和设置在所述门（20）中的第二壳（17）; 所述袋被夹在所述第一壳体（16）和所述第二壳体（17）之间，在所述薄膜之间形成用于输送液体的所述网络的导管的状态。

65. 发明申请

US20140335600A1 CELL COLLECTION METHOD 有权
标题翻译：细胞收集方法
公开(公告)号：US20140335600A1
公开(公告)日：2014-11-13
申请号：US14341543
申请日：2014-07-25
申请人： TOYO SEIKAN GROUP HOLDINGS, LTD.
发明人： Satoshi Tanaka , Ryo Suenaga , Masahiro Kuninori , Kyohei Ota
IPC分类号： C12N1/02
CPC分类号： C12N1/02 , C12M23/14 , C12M33/18
摘要： A method for collecting cells that have been cultured by using a cell culture kit in which, at least, a culture container for cultivating cells, a cell collection container for collecting cells after cultivation and a waste liquid container for collecting a culture medium after cultivation are linked to one another through a conduit, the method including transferring a culture supernatant that has been cultured from the culture container to the waste liquid container, transferring a concentrated suspension of cells from the culture container to the cell collection container, returning part of the culture supernatant discharged in the waste liquid container to the culture container and allowing cells remaining in the culture container to be suspended in a suspension of cells, and transferring again a suspension of cells in which remaining cells are suspended from the culture container to the cell collection container.
摘要翻译：采用细胞培养试剂盒收集培养细胞的方法，其中至少培养细胞用培养容器，栽培后收集细胞的细胞收集容器和培养后收集培养基的废液容器，该方法包括将从培养容器培养的培养上清液转移到废液容器，将细胞浓缩悬浮液从培养容器转移到细胞收集容器中，返回部分培养物将废液容器中的上清液排出到培养容器中，使残留在培养容器中的细胞悬浮于细胞悬浮液中，再次将剩余细胞从培养容器悬浮的细胞悬浮液转移到细胞收集容器。

66. 发明申请

US20140273176A1 Systems and Methods for Utilizing and Recovering Chitosan to Process Biological Material 有权
标题翻译：利用和回收壳聚糖处理生物材料的系统和方法
公开(公告)号：US20140273176A1
公开(公告)日：2014-09-18
申请号：US13839647
申请日：2013-03-15
申请人： Daniel Fleischer
发明人： Daniel Fleischer
IPC分类号： C12N1/02 , C08B37/08
CPC分类号： C08B37/003 , C08B37/0003 , C12M47/02 , C12P19/04 , C12P19/26
摘要： Systems and methods for processing biological material utilizing chitosan are provided herein. Methods may include the steps of reducing the pH of an amount of culture to about 5 to 7, incorporating an amount of a chitosan solution into the culture to form a mixture, wherein chitosan in the chitosan solution binds to the biological material; increasing the pH of the culture to about 7 to 10, to flocculate biological material in the culture, concentrating the flocculates, reducing the pH of the concentrated flocculates to about 3 to 5, and separating the recovered chitosan solution from the concentrated biological material.
摘要翻译：本文提供了利用壳聚糖处理生物材料的系统和方法。方法可以包括以下步骤：将培养基的pH降低至约5至7，向培养物中加入一定量的壳聚糖溶液以形成混合物，其中壳聚糖溶液中的壳聚糖结合生物材料; 将培养物的pH增加至约7〜10，使培养物中的生物材料絮凝，浓缩絮凝物，将浓缩的絮凝物的pH降低至约3至5，并将回收的壳聚糖溶液与浓缩的生物材料分离。

67. 发明授权

US08158401B2 Method for isolating and culturing unculturable microorganisms 有权
标题翻译：分离和培养不可培养微生物的方法
公开(公告)号：US08158401B2
公开(公告)日：2012-04-17
申请号：US12537646
申请日：2009-08-07
申请人： Ariel Kushmaro , Shimona Geresh , Shaul Geresh, legal representative
发明人： Ariel Kushmaro , Shimona Geresh
IPC分类号： C12Q1/68 , C12N1/00 , C12N1/02 , C12N1/06 , C12N1/20 , C12N3/00
CPC分类号： C12N1/00
摘要： The invention provides a method for isolating and culturing a previously unculturable microorganism, which comprises: (i) collecting a sample from an environmental source; (ii) counting/estimating the number of microorganisms in the sample; (iii) diluting the sample in an appropriate medium; (iv) adding a gelating agent such as to entrap one or more microorganisms within a sphere of the gelating agent; (v) coating the spheres containing the entrapped microorganism(s) with a natural or synthetic polymer to form a polymeric membrane; (vi) incubating the coated spheres in the original environment for an appropriate time; (vii) cutting the spheres and scanning for microorganisms colonies; and (viii) isolating the microorganisms, and repeating steps (iii) to (vii) until a pure clone of said previously unculturable microorganism is obtained.
摘要翻译：本发明提供了分离和培养以前不可培养的微生物的方法，其包括：（i）从环境来源收集样品; （ii）计数/估计样品中的微生物数量; （iii）将样品稀释在合适的培养基中; （iv）加入凝胶剂，例如在凝胶剂的球体内捕获一种或多种微生物; （v）用天然或合成聚合物涂覆含有捕获的微生物的球体以形成聚合物膜; （vi）将涂覆的球体在原始环境中孵育适当的时间; （vii）切割球体并扫描微生物菌落; 和（viii）分离微生物，并重复步骤（iii）至（vii），直到获得所述先前不可培养的微生物的纯克隆。

68. 发明申请

US20110201078A1 LIGAND FUNCTIONALIZED POLYMERS 有权
公开(公告)号：US20110201078A1
公开(公告)日：2011-08-18
申请号：US12986485
申请日：2011-01-07
申请人： Jerald K. Rasmussen , Catherine A. Bothof , Kannan Seshadri , Erin A. Satterwhite , Robert T. Fitzsimons , James I. Hembre , Mahfuza B. Ali
发明人： Jerald K. Rasmussen , Catherine A. Bothof , Kannan Seshadri , Erin A. Satterwhite , Robert T. Fitzsimons , James I. Hembre , Mahfuza B. Ali
IPC分类号： C12N9/00 , C08F220/56 , B32B3/26 , B01D15/38 , B01D21/01 , B01D15/08 , C12N7/02 , C07K1/32 , C12N1/20 , C12N1/02 , C12N5/00 , C07H1/06 , C07K1/14
CPC分类号： C08F220/56 , B01D15/00 , C08F20/00 , C08F26/02 , C08F220/60 , G01N33/54393 , Y10T428/12264 , Y10T428/249991
摘要： Ligand functionalized substrates, methods of making ligand functionalized substrates, and methods of using functionalized substrates are disclosed.

69. 发明申请

US20110126455A1 METHOD FOR PRODUCTION OF INOCULUM OF MICROORGANISMS OPTIMIZED AS CATALYST FOR MULTIPLE PARALLEL MINERALIZATION 审中-公开
标题翻译：优化作为多元平行矿化催化剂的微生物微生物生产方法
公开(公告)号：US20110126455A1
公开(公告)日：2011-06-02
申请号：US13056120
申请日：2009-07-16
申请人： Makoto Shinohara
发明人： Makoto Shinohara
IPC分类号： A01G31/00 , C12N1/02 , C12N1/00 , C05F11/08 , A01G1/00 , A01G17/00
CPC分类号： C05F17/00 , A01G22/00 , C05F3/00 , C05F11/10 , C12N1/14 , Y02A40/205 , Y02P20/145 , Y02W30/43
摘要： Provided is a method of producing an inoculum, which can drastically reduce a time to complete a reaction for mineralizing an organic material into nitrate nitrogen and can add a large amount of the organic material at one time in a multiple parallel mineralization for generating nitrate nitrogen as inorganic nutrients from the organic material, resulting in efficient generation of a high concentration of nitrate nitrogen and drastic reduction of the amount of a microorganism source added. The method of producing an inoculum comprises: placing water in a container that can store water therein, inoculating microorganisms capable of conducting a multiple parallel mineralization thereinto, and maintaining an environment that allows the multiple parallel mineralization to proceed in the water, thereby culturing the microorganisms capable of conducting a multiple parallel mineralization; forming a biofilm on a solid surface that contacts with the water and then collecting the biofilm; and utilizing the collected biofilm as an inoculum of the microorganisms optimized as a catalyst for the multiple parallel mineralization.
摘要翻译：提供一种生产接种物的方法，其可以显着减少完成将有机材料矿化成硝态氮的反应的时间，并且可以在多次平行的矿化中一次添加大量的有机材料以产生硝酸盐氮作为来自有机材料的无机营养物质，导致有效产生高浓度的硝酸盐氮，并大大减少了加入的微生物源的量。生产接种物的方法包括：将水放入容器中的水中，将容器内的水进行接种，接种能够进行多次平行矿化的微生物，并保持允许多次平行矿化在水中进行的环境，从而培养微生物能够进行多次平行矿化; 在与水接触的固体表面上形成生物膜，然后收集生物膜; 并利用收集的生物膜作为微生物的接种物，其被优化为用于多次平行矿化的催化剂。

70. 发明授权

US07776580B2 Magnetism based rapid cell separation 有权
标题翻译：基于磁性的快速细胞分离
公开(公告)号：US07776580B2
公开(公告)日：2010-08-17
申请号：US10538498
申请日：2002-12-31
申请人： Xu Zhang , Xin Xie , Depu Chen , Weiyang Fei , Jing Cheng
发明人： Xu Zhang , Xin Xie , Depu Chen , Weiyang Fei , Jing Cheng
IPC分类号： C12N7/02 , C12N5/00 , C12N1/02 , C12P19/34 , G01N33/50
CPC分类号： G01N33/54333 , C12N1/02 , C12N7/00 , C12N2760/16051 , C12N2795/14151 , C12Q1/24 , G01N33/54326
摘要： This invention relates generally to the field of cell seperation. In particular, the invention provides processes for isolating a target cell, cellular organelle or virus from a sample, using inter alia, nonspecific binding between a target cell, cellular organelle or virus with a magnetic microbead modified to comprise hydroxyl, carboxyl or epoxy groups.
摘要翻译：本发明一般涉及细胞分离领域。特别地，本发明提供了从样品中分离靶细胞，细胞器官细胞或病毒的方法，尤其是靶细胞，细胞器或病毒与修饰成包含羟基，羧基或环氧基团的磁性微珠之间非特异性结合。

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