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1. 发明授权

US09909090B2 Cell culture kit, and method of using cell culture kit 有权
公开(公告)号：US09909090B2
公开(公告)日：2018-03-06
申请号：US14444479
申请日：2014-07-28
申请人： Toyo Seikan Group Holdings, Ltd.
发明人： Satoshi Tanaka , Takahiko Totani , Yoichi Ishizaki
IPC分类号： C12M1/00 , C12M1/04 , C12M3/00
CPC分类号： C12M23/34 , C12M23/14 , C12M23/24 , C12M23/26 , C12M23/40 , C12M29/26
摘要： A cell culture kit for cultivating cells in a closed system includes at least one or more of each of: a culture container for cultivating cells; a culture medium storage container for storing a culture medium or the like; a cell injection container for injecting cells; and a cell collecting container for collecting a suspension of cells after cultivation; wherein the culture medium storage container is also used as a waste liquid container for collecting a culture medium after cultivation, and the culture container, the culture medium storage container, the cell injection container and the cell collecting container are linked to one another through a conduit.

2. 发明申请

US20140335608A1 CELL CULTURE KIT, AND METHOD OF USING CELL CULTURE KIT 有权
标题翻译：细胞培养箱和使用细胞培养箱的方法
公开(公告)号：US20140335608A1
公开(公告)日：2014-11-13
申请号：US14444479
申请日：2014-07-28
申请人： Toyo Seikan Group Holdings, Ltd.
发明人： Satoshi Tanaka , Takahiko Totani , Yoichi Ishizaki
IPC分类号： C12M1/00
CPC分类号： C12M23/34 , C12M23/14 , C12M23/24 , C12M23/26 , C12M23/40 , C12M29/26
摘要： A cell culture kit for cultivating cells in a closed system that comprises at least one or more of each of: a culture container for cultivating cells; a culture medium storage container for storing a culture medium or the like; a cell injection container for injecting cells; and a cell collecting container for collecting a suspension of cells after cultivation; wherein the culture medium storage container is also used as a waste liquid container for collecting a culture medium after cultivation, and the culture container, the culture medium storage container, the cell injection container and the cell collecting container are linked to one another through a conduit. Also provided is a cell culture kit including at least one or more of a sampling container that is capable of sampling part of a suspension of cells during cultivation, wherein the sampling container is linked to the culture container through a conduit.
摘要翻译：一种用于在封闭系统中培养细胞的细胞培养试剂盒，其包含以下各项中的至少一种：用于培养细胞的培养容器; 用于储存培养基等的培养基储存容器; 用于注射细胞的细胞注射容器; 以及培养后收集细胞悬浮液的细胞收集容器; 培养容器，培养容器，细胞注入容器和细胞收集容器通过导管连接到培养基存储容器中，。还提供了一种细胞培养试剂盒，其包括至少一种或多种采样容器，所述采样容器能够在培养期间取样细胞悬浮液的一部分，其中采样容器通过导管连接到培养容器。

3. 发明授权

US11414637B2 Cell culture vessel, support jig for cell culture vessel and cell culture method 有权
公开(公告)号：US11414637B2
公开(公告)日：2022-08-16
申请号：US16148229
申请日：2018-10-01
申请人： TOYO SEIKAN GROUP HOLDINGS, LTD.
发明人： Satoshi Tanaka , Ryo Suenaga , Takahiko Totani
IPC分类号： C12M1/04 , C12M1/32 , C12M1/00 , C12M1/12 , C12M1/24
摘要： A cell culture container includes a container main body comprising a gas-permeable plastic film, and an injection/discharge port. A peripheral portion of the container main body is sealed, and the container main body has a bulging shape on a top surface side of the container main body. A plurality of concave portions serving as cell culture parts are provided on a bottom surface of the container main body.

4. 发明申请

US20170283758A1 CULTURE CONTAINER AND METHOD FOR MANUFACTURING CULTURE CONTAINER 审中-公开
公开(公告)号：US20170283758A1
公开(公告)日：2017-10-05
申请号：US15627500
申请日：2017-06-20
申请人： TOYO SEIKAN GROUP HOLDINGS, LTD.
发明人： Takahiko Totani , Satoshi Tanaka
IPC分类号： C12M1/00 , C07K16/28 , C07K17/08 , C12M1/12
摘要： A culture container for activating lymphocytes includes immobilized anti-CD3 antibodies and an anti-CD3 antibody solution including anti-CD3 antibodies, wherein the culture container is formed in a bag-like shape and is formed of a soft-packaging material, the immobilized anti-CD3 antibodies are immobilized at a density of 10 to 300 ng/cm2 on one surface of opposing inner surfaces within the container, and the anti-CD3 antibody solution is enclosed in the container in an amount of 0.25 to 400 ng of the anti-CD3 antibodies in 0.1 to 800 μl of the solution per 1 cm2 of a culture surface formed of the one surface.

5. 发明申请

US20160289624A1 CULTURE CONTAINER, METHOD FOR CULTURING LYMPHOCYTES, CULTURE-CONTAINER PRODUCTION METHOD, AND SOLID-PHASING APPARATUS 审中-公开
标题翻译：培养容器，培养淋巴细胞的方法，培养容器生产方法和固相化装置
公开(公告)号：US20160289624A1
公开(公告)日：2016-10-06
申请号：US15184649
申请日：2016-06-16
申请人： TOYO SEIKAN GROUP HOLDINGS, LTD.
发明人： Takahiko Totani , Satoshi Tanaka , Takeshi Aihara , Yoichi Ishizaki
IPC分类号： C12M1/12 , C12M1/04
CPC分类号： C12M25/00 , C12M23/14 , C12M23/20 , C12M23/24 , C12M25/06 , C12N5/0636 , C12N2501/515 , C12N2513/00 , C12N2533/52 , C12N2533/54
摘要： A culture container for culturing lymphocytes includes an immobilized surface and a non-immobilized surface, wherein the culture container is formed of a gas permeable film, the immobilized surface and the non-immobilized surface are container inner surfaces facing each other, and anti-CD3 antibodies are immobilized in the immobilized surface at a concentration of 10 to 300 ng/cm2.
摘要翻译：用于培养淋巴细胞的培养容器包括固定表面和非固定化表面，其中培养容器由透气膜形成，固定化表面和非固定化表面是彼此相对的容器内表面，抗CD3 抗体以10〜300ng / cm 2的浓度固定在固定化的表面。

6. 发明申请

US20220259538A1 CULTURE CONTAINER, METHOD FOR MANUFACTURING CULTURE CONTAINER, LAMINATED STRUCTURE, AND METHOD FOR MANUFACTURING LAMINATED STRUCTURE 有权
公开(公告)号：US20220259538A1
公开(公告)日：2022-08-18
申请号：US17738246
申请日：2022-05-06
申请人： TOYO SEIKAN GROUP HOLDINGS, LTD.
发明人： Takahiko Totani , Satoshi Tanaka , Yosuke Matsuoka
IPC分类号： C12M1/00 , C12M1/12
摘要： A culture container made of a flexible packaging material where the culture container includes a first side surface portion and a second side surface portion that are mutually opposed. The culture container also includes at least an inner surface side of the first side surface portion which has a surface treatment such that, when a contact angle hysteresis is defined as an advance contact angle—a retreat contact angle at a time when water slips, a contact angle hysteresis of a surface on the inner surface side of the first side surface portion becomes larger than a contact angle hysteresis of a surface on an inner surface side of the second side surface portion. The culture container further includes a membrane made of a cell adhesion inhibitor that is formed on the inner surface side of the first side surface portion.

7. 发明授权

US09574168B2 Cell collection method 有权
标题翻译：细胞收集方法
公开(公告)号：US09574168B2
公开(公告)日：2017-02-21
申请号：US14341543
申请日：2014-07-25
申请人： Toyo Seikan Group Holdings, Ltd.
发明人： Satoshi Tanaka , Ryo Suenaga , Masahiro Kuninori , Kyohei Ota
IPC分类号： C12N1/02 , C12M1/00 , C12M1/26
CPC分类号： C12N1/02 , C12M23/14 , C12M33/18
摘要： A method for collecting cells that have been cultured by using a cell culture kit in which, at least, a culture container for cultivating cells, a cell collection container for collecting cells after cultivation and a waste liquid container for collecting a culture medium after cultivation are linked to one another through a conduit is provided. The method includes transferring a culture supernatant that has been cultured from the culture container to the waste liquid container, transferring a concentrated suspension of cells from the culture container to the cell collection container, returning part of the culture supernatant discharged in the waste liquid container to the culture container and allowing cells remaining in the culture container to be suspended in a suspension of cells, and transferring again a suspension of cells in which remaining cells are suspended from the culture container to the cell collection container.
摘要翻译：采用细胞培养试剂盒收集培养细胞的方法，其中至少培养细胞用培养容器，栽培后收集细胞的细胞收集容器和培养后收集培养基的废液容器，提供了通过导管彼此连接的。该方法包括将从培养容器培养的培养上清液转移到废液容器，将细胞浓缩悬浮液从培养容器转移到细胞收集容器中，将排出到废液容器中的部分培养上清液返回到将培养容器保持在培养容器中的细胞悬浮在细胞悬浮液中，再次将残留的细胞从培养容器悬浮的细胞悬浮液转移到细胞收集容器。

8. 发明申请

US20210115376A1 CULTURE CONTAINER BASE MATERIAL, CULTURE CONTAINER, AND PRODUCTION METHOD OF CULTURE CONTAINER BASE MATERIAL 有权
公开(公告)号：US20210115376A1
公开(公告)日：2021-04-22
申请号：US17087913
申请日：2020-11-03
申请人： TOYO SEIKAN GROUP HOLDINGS, LTD.
发明人： Yosuke Matsuoka , Satoshi Tanaka , Takahiko Totani , Takaharu Nishiyama
IPC分类号： C12M1/12 , C12N1/00
摘要： Provided is a culture container base material made of a polyolefin material, capable of readily forming a culture container for culturing adherent cells. The culture container base material is for culturing the adherent cells and made of the polyolefin material. At least a part of a surface of the base material is subjected to a surface treatment, where the surface of the base material is a culture surface of the culture container, and the surface subjected to the surface treatment has a static water contact angle of greater than 80° and a receding contact angle of less than 53°.

9. 发明申请

US20200148992A1 CULTURE CONTAINER, METHOD FOR MANUFACTURING CULTURE CONTAINER, LAMINATED STRUCTURE, AND METHOD FOR MANUFACTURING LAMINATED STRUCTURE 审中-公开
公开(公告)号：US20200148992A1
公开(公告)日：2020-05-14
申请号：US16743911
申请日：2020-01-15
申请人： TOYO SEIKAN GROUP HOLDINGS, LTD.
发明人： Takahiko Totani , Satoshi Tanaka , Yosuke Matsuoka
IPC分类号： C12M1/00 , C12M1/12
摘要： A culture container made of a flexible packaging material where the culture container includes a first side surface portion and a second side surface portion that are mutually opposed. The culture container also includes at least an inner surface side of the first side surface portion which has a surface treatment such that, when a contact angle hysteresis is defined as an advance contact angle—a retreat contact angle at a time when water slips, a contact angle hysteresis of a surface on the inner surface side of the first side surface portion becomes larger than a contact angle hysteresis of a surface on an inner surface side of the second side surface portion. The culture container further includes a membrane made of a cell adhesion inhibitor that is formed on the inner surface side of the first side surface portion.

10. 发明申请

US20190127676A1 MULTILAYER FILM AND CELL CULTURE CONTAINER 审中-公开
公开(公告)号：US20190127676A1
公开(公告)日：2019-05-02
申请号：US16232865
申请日：2018-12-26
申请人： TOYO SEIKAN GROUP HOLDINGS, LTD.
发明人： Satoshi Tanaka , Takahiko Totani , Yoichi Ishizaki , Kyohei Ota , Ryo Suenaga , Masahiro Kuninori
IPC分类号： C12M1/04 , B32B1/02 , C12M1/00 , B32B27/32 , B32B1/08 , B32B7/02 , B32B27/08
摘要： Provided are a multilayer film having excellent gas permeability and excellent handling properties and hence is suited for forming a cell culture container, and a cell culture container formed by using the same.A multilayer film used for forming a cell culture container, comprising: a base material composed of a polyethylene-based resin having a density of 0.87 g/cm3 to 0.90 g/cm3; and an inner layer composed of a polyethylene-based resin having a density of 0.896 g/cm3 to 0.93 g/cm3 and forming a cell culture icy surface. A cell culture container is formed by using this multilayer film.

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