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1. 发明授权

US08158401B2 Method for isolating and culturing unculturable microorganisms 有权
标题翻译：分离和培养不可培养微生物的方法
公开(公告)号：US08158401B2
公开(公告)日：2012-04-17
申请号：US12537646
申请日：2009-08-07
申请人： Ariel Kushmaro , Shimona Geresh , Shaul Geresh, legal representative
发明人： Ariel Kushmaro , Shimona Geresh
IPC分类号： C12Q1/68 , C12N1/00 , C12N1/02 , C12N1/06 , C12N1/20 , C12N3/00
CPC分类号： C12N1/00
摘要： The invention provides a method for isolating and culturing a previously unculturable microorganism, which comprises: (i) collecting a sample from an environmental source; (ii) counting/estimating the number of microorganisms in the sample; (iii) diluting the sample in an appropriate medium; (iv) adding a gelating agent such as to entrap one or more microorganisms within a sphere of the gelating agent; (v) coating the spheres containing the entrapped microorganism(s) with a natural or synthetic polymer to form a polymeric membrane; (vi) incubating the coated spheres in the original environment for an appropriate time; (vii) cutting the spheres and scanning for microorganisms colonies; and (viii) isolating the microorganisms, and repeating steps (iii) to (vii) until a pure clone of said previously unculturable microorganism is obtained.
摘要翻译：本发明提供了分离和培养以前不可培养的微生物的方法，其包括：（i）从环境来源收集样品; （ii）计数/估计样品中的微生物数量; （iii）将样品稀释在合适的培养基中; （iv）加入凝胶剂，例如在凝胶剂的球体内捕获一种或多种微生物; （v）用天然或合成聚合物涂覆含有捕获的微生物的球体以形成聚合物膜; （vi）将涂覆的球体在原始环境中孵育适当的时间; （vii）切割球体并扫描微生物菌落; 和（viii）分离微生物，并重复步骤（iii）至（vii），直到获得所述先前不可培养的微生物的纯克隆。

2. 发明申请

US20100120037A1 METHOD FOR ISOLATING AND CULTURING UNCULTURABLE MICROORGANISMS 有权
标题翻译：用于分离和培养不可培养微生物的方法
公开(公告)号：US20100120037A1
公开(公告)日：2010-05-13
申请号：US12537646
申请日：2009-08-07
申请人： Ariel Kushmaro , Shimona Geresh , Shaul Geresh
发明人： Ariel Kushmaro , Shimona Geresh , Shaul Geresh
IPC分类号： C12Q1/68 , C12N1/00 , C12N1/20 , C40B40/02
CPC分类号： C12N1/00
摘要： The invention provides a method for isolating and culturing a previously unculturable microorganism, which comprises: (i) collecting a sample from an environmental source; (ii) counting/estimating the number of microorganisms in the sample; (iii) diluting the sample in an appropriate medium; (iv) adding a gelating agent such as to entrap one or more microorganisms within a sphere of the gelating agent; (v) coating the spheres containing the entrapped microorganism(s) with a natural or synthetic polymer to form a polymeric membrane; (vi) incubating the coated spheres in the original environment for an appropriate time; (vii) cutting the spheres and scanning for microorganisms colonies; and (viii) isolating the microorganisms, and repeating steps (iii) to (vii) until a pure clone of said previously unculturable microorganism is obtained.
摘要翻译：本发明提供了分离和培养以前不可培养的微生物的方法，其包括：（i）从环境来源收集样品; （ii）计数/估计样品中的微生物数量; （iii）将样品稀释在合适的培养基中; （iv）加入凝胶剂，例如在凝胶剂的球体内捕获一种或多种微生物; （v）用天然或合成聚合物涂覆含有捕获的微生物的球体以形成聚合物膜; （vi）将涂覆的球体在原始环境中孵育适当的时间; （vii）切割球体并扫描微生物菌落; 和（viii）分离微生物，并重复步骤（iii）至（vii），直到获得所述先前不可培养的微生物的纯克隆。

3. 发明申请

US20060240506A1 Method for isolating and culturing unculturable microorganisms 审中-公开
标题翻译：分离和培养不可培养微生物的方法
公开(公告)号：US20060240506A1
公开(公告)日：2006-10-26
申请号：US10527076
申请日：2003-09-03
申请人： Ariel Kushmaro , Shimona Geresh , Shaul Geresh
发明人： Ariel Kushmaro , Shimona Geresh , Shaul Geresh
IPC分类号： C12Q1/04 , C12N1/20
CPC分类号： C12N1/00
摘要： The invention provides a method for isolating and culturing a previously unculturable microorganism, which comprises: (i) collecting a sample from an environmental source; (ii) counting/estimating the number of microorganisms in the sample; (iii) diluting the sample in an appropriate medium; (iv) adding a gelating agent such as to entrap one or more microorganisms within a sphere of the gelating agent; (v) coating the spheres containing the entrapped microorganism(s) with a natural or synthetic polymer to form a polymeric membrane; (vi) incubating the coated spheres in the original environment for an appropriate time; (vii) cutting the spheres and scanning for microorganisms colonies; and (viii) isolating the microorganisms, and repeating steps (iii) to (vii) until a pure clone of said previously unculturable microorganism is obtained.
摘要翻译：本发明提供了分离和培养以前不可培养的微生物的方法，其包括：（i）从环境来源收集样品; （ii）计数/估计样品中的微生物数量; （iii）将样品稀释在合适的培养基中; （iv）加入凝胶剂，例如在凝胶剂的球体内捕获一种或多种微生物; （v）用天然或合成聚合物涂覆含有捕获的微生物的球体以形成聚合物膜; （vi）将涂覆的球体在原始环境中孵育适当的时间; （vii）切割球体并扫描微生物菌落; 和（viii）分离微生物，并重复步骤（iii）至（vii），直到获得所述先前不可培养的微生物的纯克隆。

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