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61. 发明申请

US20070077662A1 APPARATUS AND METHOD FOR MEASURING 8-OHdG 审中-公开
标题翻译：用于测量8-OHdG的装置和方法
公开(公告)号：US20070077662A1
公开(公告)日：2007-04-05
申请号：US11470955
申请日：2006-09-07
申请人： Yohei Inaba , Kenji Yokoyama , Isao Karube , Narushi Ito
发明人： Yohei Inaba , Kenji Yokoyama , Isao Karube , Narushi Ito
IPC分类号： G01N1/18
CPC分类号： G01N30/88 , G01N2030/8813 , Y10T436/255
摘要： To provide a method for effectively and simply separating and condensing 8-OHdG which is present by a trace amount in body fluid, particularly in urine, and is frequently mingled with foreign substances with their peaks appearing around the peak thereof, a simple measurement method of 8-OHdG, and an apparatus for the measurement. [Solution]The present invention provides a method for effectively and simply separating and condensing 8-OHdG by an optimum combination of chromatographies. Specifically, the present invention comprises a method for separating and condensing 8-hydroxy-2′-deoxyguanosine (8-OHdG) from a body fluid, wherein a urine sample is contacted with a hydrophobic adsorbent which has, as the functional group, a straight chain hydrocarbon group having a carbon number of 6-30 and has a C % of 18% or less to capture the 8-OHdG. The method for measuring 8-OHdG and the apparatus for the measurement method according to the present invention use electrochemical reaction to measure the amount of 8-OHdG in the sample.
摘要翻译： <待解决的问题>提供一种有效且简单地分离和冷凝8-OHdG的方法，其通过微量的体液，特别是尿中存在，并且经常与外来物质混合，其峰出现在其峰顶周围，8-OHdG的简单测量方法和用于测量的装置。 [解决方案]本发明提供了通过色谱的最佳组合有效且简单地分离和冷凝8-OHdG的方法。具体地说，本发明包括从体液中分离和浓缩8-羟基-2'-脱氧鸟苷（8-OHdG）的方法，其中使尿样与疏水性吸附剂接触，所述疏水性吸附剂具有作为官能团的直链碳原子数为6〜30的碳原子数为8〜8的C-C的烃基，捕捉8-OHdG。根据本发明的用于测量8-OHdG的方法和测量方法的装置使用电化学反应来测量样品中8-OHdG的量。

62. 发明申请

US20070000311A1 Method for measuring substance having affinity 审中-公开
标题翻译：测量具有亲和性的物质的方法
公开(公告)号：US20070000311A1
公开(公告)日：2007-01-04
申请号：US10561105
申请日：2004-06-11
申请人： Isao Karube , Keisuke Iwata
发明人： Isao Karube , Keisuke Iwata
IPC分类号： G01N30/00
CPC分类号： G01N15/12 , G01N15/04 , G01N33/561
摘要： The binding reaction between an affinity substance to be measured and a binding partner having binding affinity for the affinity substance is measured based on agglutination reactions. Carrier particles bound to the binding partner are allowed to bind to the affinity substance in an electric field. Evaluation is achieved by counting the level of agglutinated carrier particles using three-dimensional particle information of the particles as an indicator. The use of three-dimensional information as an indicator enables the presence of a biologically specific reactive substance to be detected or measured in a manner that is more convenient and rapid, and has a higher sensitivity than the conventional measurement methods.
摘要翻译：基于凝集反应测定待测亲和性物质与亲和物质结合亲和力的结合对象之间的结合反应。与结合配偶体结合的载体颗粒在电场中与亲和物质结合。通过使用颗粒的三维粒子信息作为指标计数凝集载体颗粒的水平来实现评估。使用三维信息作为指标，可以以更方便快捷的方式检测或测量生物特异性反应物质的存在，并且具有比常规测量方法更高的灵敏度。

63. 发明申请

US20060251171A1 Image coding device and image coding method 有权
标题翻译：图像编码装置和图像编码方法
公开(公告)号：US20060251171A1
公开(公告)日：2006-11-09
申请号：US11367569
申请日：2006-03-06
申请人： Tomokazu Murakami , Isao Karube , Hiroaki Ito
发明人： Tomokazu Murakami , Isao Karube , Hiroaki Ito
IPC分类号： H04N11/02 , H04N11/04 , H04N7/12 , H04B1/66
CPC分类号： H04N19/137 , H04N19/14 , H04N19/56
摘要： In the motion search in the inter-frame prediction coding, parameters necessary for the motion search, such as sample-point spacing and sample-point number in the step search, are controlled using a unit for calculating the complexity of an image and a unit for estimating the movement amount of the image. This configuration makes it possible to provide the scheme for searching for the high-speed and high-accuracy motion vector, and the image coding device for performing this motion search.
摘要翻译：在帧间预测编码中的运动搜索中，使用用于计算图像和单位的复杂度的单元来控制运动搜索所需的参数（诸如步进搜索中的采样点间隔和采样点数）用于估计图像的移动量。该配置使得可以提供用于搜索高速和高精度运动矢量的方案以及用于执行该运动搜索的图像编码装置。

64. 发明授权

US07087149B1 Biosensor 有权
标题翻译：生物传感器
公开(公告)号：US07087149B1
公开(公告)日：2006-08-08
申请号：US09958671
申请日：2000-04-13
申请人： Hitoshi Muguruma , Atsunori Hiratsuka , Isao Karube
发明人： Hitoshi Muguruma , Atsunori Hiratsuka , Isao Karube
IPC分类号： G01N27/327 , C08F2/46 , C12Q1/00
CPC分类号： C12Q1/002
摘要： A novel biosensor was accomplished using a plasma-polymerized membrane. The biosensor of the present invention is a high-performance biosensor produced by a simpler method and applicable to a wide range of fields.
摘要翻译：使用等离子体聚合膜完成了新颖的生物传感器。本发明的生物传感器是通过更简单的方法制造并适用于广泛领域的高性能生物传感器。

65. 发明申请

US20060137983A1 Method of controlling migration of substance 有权
标题翻译：控制物质迁移的方法
公开(公告)号：US20060137983A1
公开(公告)日：2006-06-29
申请号：US10533015
申请日：2003-10-28
申请人： Kazuyoshi Yano , Isao Karube , Atsunori Hiratsuka , Shuo-Wen Tsai
发明人： Kazuyoshi Yano , Isao Karube , Atsunori Hiratsuka , Shuo-Wen Tsai
IPC分类号： C07K1/26 , B01D57/02 , G01N27/447
CPC分类号： B01D57/02 , G01N27/44773 , G01N27/44791
摘要： Transfer of fluid substances, and/or substances comprised in fluid substances, is controlled by introducing a separation medium, which prevents transfer of the fluid substances, and/or the substances comprised in the fluid substances, to an intermediate cavity connecting a first cavity and a second cavity; and introducing a connection medium to replace the separation medium and thereby start substance transfer to the second cavity. Substance transfer may be readily controlled without relying on mechanical means. Based on the present invention, two-dimensional electrophoretic analysis can be readily implemented on a chip.
摘要翻译：通过引入分离介质来控制流体物质和/或包含在流体物质中的物质，该分离介质防止流体物质和/或包含在流体物质中的物质转移到连接第一空腔和第二腔; 并引入连接介质以更换分离介质，从而开始物质转移到第二腔。可以容易地控制物质转移，而不依赖于机械装置。基于本发明，可以容易地在芯片上实现二维电泳分析。

66. 发明申请

US20060113189A1 Method for separating substances 审中-公开
标题翻译：分离物质的方法
公开(公告)号：US20060113189A1
公开(公告)日：2006-06-01
申请号：US10526213
申请日：2003-09-05
申请人： Atsunori Hiratsuka , Kazuyoshi Yano , Isao Karube , Shuo-Wen Tsai , Kenji Yokoyama , Satoshi Koide , Takuo Akimoto
发明人： Atsunori Hiratsuka , Kazuyoshi Yano , Isao Karube , Shuo-Wen Tsai , Kenji Yokoyama , Satoshi Koide , Takuo Akimoto
IPC分类号： C07K1/26 , G01N27/447
CPC分类号： B82Y30/00 , G01N27/447 , G01N27/44752
摘要： An objective of the present invention is to provide electrophoretic separation methods and devices that enable the various features of a substrate surface that comes in contact with an electrophoresis medium to be controlled. The present invention provides methods for electrophoresing substances, which comprises the steps of: (a) adding a substance to be analyzed to an electrophoresis medium retained in a substrate, whose surface that has come in contact with the electrophoresis medium has been coated with a polymer membrane; and (b) adding electrophoretic pressure to the electrophoresis medium. For example, the use of a plasma-polymerized membrane allows the formation of a membrane with homogeneous quality and thickness on the surface of an arbitrary shape. In addition, desired characteristics can be conferred on the surface through selection of monomeric substances. Protein adsorption onto micro-chips can be effectively prevented as well.
摘要翻译：本发明的目的是提供一种电泳分离方法和装置，其使得能够控制与电泳介质接触的基底表面的各种特征。本发明提供了用于电泳物质的方法，其包括以下步骤：（a）将待分析的物质加入保留在基质中的电泳介质中，其中与电泳介质接触的表面已经涂覆有聚合物膜; 和（b）向电泳介质中加入电泳压力。例如，使用等离子体聚合膜允许在任意形状的表面上形成均匀质量和厚度的膜。此外，通过选择单体物质可以赋予表面所需的特性。可以有效地防止蛋白质在微芯片上的吸附。

67. 发明授权

US06753171B2 Site-specific cell perforation technique 失效
标题翻译：位点特异性细胞穿孔技术
公开(公告)号：US06753171B2
公开(公告)日：2004-06-22
申请号：US09623970
申请日：2000-12-28
申请人： Isao Karube , Takashi Saitoh
发明人： Isao Karube , Takashi Saitoh
IPC分类号： C12N1509
CPC分类号： C12N15/89 , C12M35/00 , C12N13/00
摘要： A technique for controlling membrane denaturation reactions other than physical shear force was developed. For example, the present invention provides, a method for causing membrane disruption at a specific site by reacting a stimulus such as light with a compound that is activated by the stimulus, where the reaction occurs on a membrane such as a biomembrane. It also provides a membrane structure such as cells in which a specific site has been disrupted, which are obtained by the present method. Introduction of substances such as genes also became possible by using this membrane structure. Further provided is a membrane-destroying member for disrupting a membrane at a specific site. Thus, the present invention enabled, for example, easy membrane penetration using components constituting microelectrodes, micromanipulators, and microinjectors, which were conventionally hardly usable in penetrating cell membranes.
摘要翻译：开发了除物理剪切力之外控制膜变性反应的技术。例如，本发明提供了通过使诸如光的刺激与由刺激激活的化合物反应而使特定部位的膜破裂的方法，其中反应发生在诸如生物膜的膜上。还提供了通过本方法获得的膜结构，例如其中特异性位点已被破坏的细胞。使用这种膜结构也可以引入基因等物质。还提供了用于破坏特定部位的膜的破坏薄膜的部件。因此，本发明能够例如使用通常难以用于穿透细胞膜的微电极，显微操纵器和显微注射器的构成的薄膜穿透。

68. 发明授权

US5556775A Method of producing .beta.-cyclodextrin 失效
标题翻译：生产β-环糊精的方法
公开(公告)号：US5556775A
公开(公告)日：1996-09-17
申请号：US211920
申请日：1994-06-22
申请人： Isao Karube , Nobuyuki Yoshida
发明人： Isao Karube , Nobuyuki Yoshida
IPC分类号： C08B37/16 , C12P19/18
CPC分类号： C12P19/18
摘要： The present invention; is a method of producing .beta.-cyclodextrin at a higher efficiency from a raw material other than starch, comprising making a malto-oligosaccharide with 2 to 10 glucoses co-exist with cyclodextrin glucanotransferase in a solution containing an organic solvent which can precipitate 50% or more of .beta.-cyclodextrin when an excessive amount of the solvent is added to the solution of the .beta.-cyclodextrin and effecting the reaction at a lower temperature than 40.degree. C.
摘要翻译： PCT No.PCT / JP93 / 01193 Sec。 371日期：1994年6月22日 102（e）1994年6月22日PCT 1993年8月25日提交本发明; 是从淀粉以外的原料以更高的效率生产β-环糊精的方法，包括制备具有2至10个葡萄糖的麦芽寡糖与环糊精葡聚糖转移酶在含有有机溶剂的溶液中共存，所述有机溶剂可沉淀50％或当将过量的溶剂加入到β-环糊精的溶液中并在低于40℃的温度下进行反应时，更多的β-环糊精

69. 发明授权

US4350763A Method for determining biochemical oxygen demand 失效
标题翻译：确定生化需氧量的方法
公开(公告)号：US4350763A
公开(公告)日：1982-09-21
申请号：US138121
申请日：1980-04-07
申请人： Shuichi Suzuki , Isao Karube
发明人： Shuichi Suzuki , Isao Karube
IPC分类号： G01N33/18
CPC分类号： G01N33/1806 , Y10S435/817
摘要： The biochemical oxygen demand (BOD) of an aqueous liquid contaminated with organic matter is determined by contacting a sample solution with elementary oxygen and with immobilized microorganisms capable of aerobically metabolizing the organic matter and of thereby consuming the oxygen. The rate of oxygen consumption under otherwise uniform conditions is as precise a measure of BOD as the conventional five-day test, but is determined within less than two hours, usually less than 30 minutes. A membrane-type oxygen-sensitive electrode is employed for sensing the rate of oxygen consumption and generates an output signal which is readily correlated with the BOD of the tested liquid.
摘要翻译：用有机物质污染的含水液体的生物化学需氧量（BOD）是通过使样品溶液与基本氧接触的固体微生物和能够有机代谢有机物质并从而消耗氧气的固定化微生物来确定的。在其他均匀条件下的氧消耗速率与常规五天试验一样精确地测量BOD，但是在不到两小时，通常少于30分钟内测定。使用膜式氧敏电极来感测氧消耗的速率，并产生容易与被测液体的BOD相关的输出信号。

70. 发明授权

US4297173A Method for determining ammonia and sensor therefor 失效
标题翻译：氨和传感器的测定方法
公开(公告)号：US4297173A
公开(公告)日：1981-10-27
申请号：US152177
申请日：1980-05-22
申请人： Motohiko Hikuma , Tatsuru Kubo , Takeo Yasuda , Isao Karube , Shuichi Suzuki
发明人： Motohiko Hikuma , Tatsuru Kubo , Takeo Yasuda , Isao Karube , Shuichi Suzuki
IPC分类号： C12Q1/00 , C12Q1/12 , G01N27/30
CPC分类号： C12Q1/12 , C12Q1/001 , Y10S435/817 , Y10T436/175383
摘要： The concentration of ammonia in an aqueous liquid is determined by contacting a sample of the liquid with dissolved oxygen and with a microbial electrode comprising an oxygen-sensitive electrode, a porous membrane, and nitrifying bacteria confined or immobilised by the membrane which are in direct or close contact with the diaphragm of the electrode. The rate of oxygen consumption under otherwise uniform conditions is as precise a measure of concentration of ammonia as a conventional colorimetric method and distillation-titration method.
摘要翻译：通过使液体样品与溶解的氧接触并与包含氧敏电极，多孔膜和由膜限制或固定的硝化细菌的微生物电极来测定氨在水中的浓度，所述微生物电极直接或与电极的隔膜紧密接触。在其他均匀条件下的氧消耗速率与常规比色法和蒸馏滴定法精确测量氨浓度。

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