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51. 发明申请

US20120107808A1 High throughput detection of gene-specific hydroxymethylation 审中-公开
标题翻译：高通量检测基因特异性羟甲基化
公开(公告)号：US20120107808A1
公开(公告)日：2012-05-03
申请号：US12925699
申请日：2010-10-27
申请人： Weiwei Li , Jessica Li
发明人： Weiwei Li , Jessica Li
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6804 , C12Q1/682 , C12Q1/6827 , C12Q2537/164 , C12Q2563/155 , C12Q2565/501 , C12Q2563/131
摘要： This invention provides a method for the detection of hydroxymethylation patterns in a DNA sample, especially in genetic regions. A test sample containing hydroxymethylated DNA is hybridized to capture oligonucleotides immobilized on a solid phase. The hydroxymethylated DNA in hybrid is detected using an antibody which specifically recognizes hydroxymethylcytosine structure the marker of DNA hydroxymethylation-followed by immuno-signal amplification. The present invention provides a method to detect gene-specific hydroxymethylation in a simple, rapid and high throughput format with high specificity and sensitivity.
摘要翻译：本发明提供了检测DNA样品中羟甲基化模式的方法，特别是在遗传区域中。将含有羟甲基化DNA的测试样品杂交以捕获固定在固相上的寡核苷酸。使用特异性识别羟甲基胞嘧啶结构的DNA羟甲基化标记的抗体 - 随后进行免疫信号扩增检测杂交体中的羟甲基化DNA。本发明提供以高特异性和灵敏度以简单，快速和高通量格式检测基因特异性羟甲基化的方法。

52. 发明授权

US08124751B2 Hybridization chain reaction amplification for in situ imaging 有权
标题翻译：用于原位成像的杂交链反应扩增
公开(公告)号：US08124751B2
公开(公告)日：2012-02-28
申请号：US12790379
申请日：2010-05-28
申请人： Niles A. Pierce , Robert Dirks , Scott E. Fraser
发明人： Niles A. Pierce , Robert Dirks , Scott E. Fraser
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C12Q1/682 , C12Q1/6818 , C12Q1/6841 , Y10T436/143333 , C12Q2563/107 , C12Q2537/149 , C12Q2525/301
摘要： The present invention relates to the use of fluorescently labeled nucleic acid probes to identify and image analytes in a biological sample. In the preferred embodiments, a probe is provided that comprises a target region able to specifically bind an analyte of interest and an initiator region that is able to initiate polymerization of nucleic acid monomers. After contacting a sample with the probe, labeled monomers are provided that form a tethered polymer. Triggered probes and self-quenching monomers can be used to provide active background suppression.
摘要翻译：本发明涉及使用荧光标记的核酸探针来鉴定和成像生物样品中的分析物。在优选的实施方案中，提供了包含能够特异性结合目标分析物的靶区域和能够引发核酸单体聚合的引发区域的探针。在将样品与探针接触后，提供形成系留聚合物的标记单体。触发的探针和自熄单体可用于提供活性背景抑制。

53. 发明授权

US08114962B2 Method of detection of nucleic acids with a specific sequence composition 失效
标题翻译：检测具有特定序列组成的核酸的方法
公开(公告)号：US08114962B2
公开(公告)日：2012-02-14
申请号：US12493877
申请日：2009-06-29
申请人： Susan Weininger , Arthur M. Weininger
发明人： Susan Weininger , Arthur M. Weininger
IPC分类号： A61K38/16 , G01N33/53
CPC分类号： C12Q1/6811 , C12Q1/6804 , C12Q1/6813 , C12Q1/682 , C12Q1/6827 , C12Q1/6853 , C12Q1/703 , C12Q1/708 , C12Q2537/161 , C12Q2537/149 , C12Q2537/143 , C12Q2525/301
摘要： This invention is a novel method for detecting and localizing specific nucleic acid sequences in a sample with a high degree of sensitivity and specificity. The method and novel compositions used in the method involve the use of Probe Nucleic Acids, the production of nucleic acid binding regions and the use of nucleic acid Target Binding Assemblies to detect and localize specific Target Nucleic Acids. The detection and localization of the Target Nucleic Acid is accomplished even in the presence of nucleic acids which have similar sequences. The method provides for a high degree of amplification of the signal produced by each specific binding event. In particular, methods and compositions are presented for the detection of HIV and HPV nucleic acid in samples. These methods and compositions find use in diagnosis of disease, genetic monitoring, forensics, and analysis of nucleic acid mixtures. Some of the novel compositions used in the detection method are useful in preventing or treating pathogenic conditions.
摘要翻译：本发明是用于以高灵敏度和特异性检测和定位样品中特异性核酸序列的新方法。该方法中使用的方法和新颖的组合涉及使用探针核酸，核酸结合区的产生以及使用核酸靶结合组件来检测和定位特异性靶核酸。靶核酸的检测和定位即使在具有相似序列的核酸的存在下也能实现。该方法提供了通过每个特定结合事件产生的信号的高度放大。特别地，提出了用于在样品中检测HIV和HPV核酸的方法和组合物。这些方法和组合物可用于诊断疾病，遗传监测，取证和核酸混合物的分析。用于检测方法的一些新型组合物可用于预防或治疗病原性病症。

54. 发明授权

US08080375B2 Methods and kits for detecting an enzyme capable of modifying a nucleic acid 失效
标题翻译：用于检测能够修饰核酸的酶的方法和试剂盒
公开(公告)号：US08080375B2
公开(公告)日：2011-12-20
申请号：US11914697
申请日：2006-05-18
申请人： Stuart Wilson , John Christopher Stanley , Sharon Banin
发明人： Stuart Wilson , John Christopher Stanley , Sharon Banin
IPC分类号： C12Q1/68
CPC分类号： G01N33/57434 , C12Q1/42 , C12Q1/682 , G01N33/573 , C12Q2563/179 , C12Q2521/525
摘要： An improved method of detecting an enzyme in a sample, which enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule, thereby conferring the nucleic acid molecule with the ability to be extended to generate a novel detectable nucleic acid molecule, comprises the steps of allowing the sample to be tested for the presence of the enzyme to interact with the nucleic acid molecule; and testing for interaction of the enzyme with the nucleic acid molecule by detecting the novel nucleic acid molecule generated only in the presence of the enzyme. The preferred enzyme is a phosphatase. The methods have a number of applications, for example in enhancing the sensitivity of immunoassays, for detecting pathogen associated phosphatase, for diagnosing certain conditions and for detecting specific contaminants in a sample.
摘要翻译：一种检测样品中酶的改进方法，该酶能够向或从核酸分子添加或除去化学部分，从而赋予具有延伸以产生新的可检测核酸分子的能力的核酸分子包括以下步骤：使样品测试酶的存在与核酸分子相互作用; 并通过检测仅在酶存在下产生的新的核酸分子来测试酶与核酸分子的相互作用。优选的酶是磷酸酶。该方法具有许多应用，例如增强免疫测定的灵敏度，用于检测病原体相关磷酸酶，用于诊断某些条件并检测样品中的特定污染物。

55. 发明申请

US20110263045A1 Method of Detection of Nucleic Acids With a Specific Sequence Composition 审中-公开
标题翻译：用特定序列组成检测核酸的方法
公开(公告)号：US20110263045A1
公开(公告)日：2011-10-27
申请号：US13093127
申请日：2011-04-25
申请人： Susan Weininger , Arthur M. Weininger
发明人： Susan Weininger , Arthur M. Weininger
IPC分类号： G01N33/53
CPC分类号： C12Q1/6811 , C12Q1/6804 , C12Q1/6813 , C12Q1/682 , C12Q1/6827 , C12Q1/6853 , C12Q1/703 , C12Q1/708 , C12Q2537/161 , C12Q2537/149 , C12Q2537/143 , C12Q2525/301
摘要： This invention is a novel method for detecting and localizing specific nucleic acid sequences in a sample with a high degree of sensitivity and specificity. The method and novel compositions used in the method involve the use of Probe Nucleic Acids, the production of nucleic acid binding regions and the use of nucleic acid Target Binding Assemblies to detect and localize specific Target Nucleic Acids. The detection and localization of the Target Nucleic Acid is accomplished even in the presence of nucleic acids which have similar sequences. The method provides for a high degree of amplification of the signal produced by each specific binding event. In particular, methods and compositions are presented for the detection of HIV and HPV nucleic acid in samples. These methods and compositions find use in diagnosis of disease, genetic monitoring, forensics, and analysis of nucleic acid mixtures. Some of the novel compositions used in the detection method are useful in preventing or treating pathogenic conditions.
摘要翻译：本发明是用于以高灵敏度和特异性检测和定位样品中特异性核酸序列的新方法。该方法中使用的方法和新颖的组合涉及使用探针核酸，核酸结合区的产生以及使用核酸靶结合组件来检测和定位特异性靶核酸。靶核酸的检测和定位即使在具有相似序列的核酸的存在下也能实现。该方法提供了通过每个特定结合事件产生的信号的高度放大。特别地，提出了用于在样品中检测HIV和HPV核酸的方法和组合物。这些方法和组合物可用于诊断疾病，遗传监测，取证和核酸混合物的分析。用于检测方法的一些新型组合物可用于预防或治疗病原性病症。

56. 发明申请

US20110256536A1 Two stage nucleic acid amplification using an amplification oligomer 有权
标题翻译：使用扩增寡聚物的两阶段核酸扩增
公开(公告)号：US20110256536A1
公开(公告)日：2011-10-20
申请号：US13066727
申请日：2011-04-21
申请人： Gary McMaster , Yunqing Ma
发明人： Gary McMaster , Yunqing Ma
IPC分类号： C12Q1/68 , G01N27/447 , G01N33/559 , G01N33/53 , C07H21/00
CPC分类号： C12Q1/682 , C12Q1/6837 , C12Q2563/103 , C12Q2537/162 , C12Q2531/113
摘要： This invention provides methods, compositions and systems to detect a nucleic acid of interest in a two-stage amplification. The two-stage amplification begins with a first non-enzymatic accumulation of an amplification oligomer that is the target substrate for a second nucleic acid amplification or assay. Two or more amplification oligomers can be used to allow multiplexed amplifications of two or more nucleic acids of interest with deconvolution based on unique detection signals or unique signal locations.
摘要翻译：本发明提供在两级扩增中检测感兴趣的核酸的方法，组合物和系统。两步扩增开始于作为第二次核酸扩增或测定的靶底物的扩增寡聚物的第一非酶积累。可以使用两个或更多个扩增寡聚体，以基于独特的检测信号或唯一的信号位置，通过去卷积来允许两个或多个感兴趣的核酸的多重扩增。

57. 发明授权

US08030094B2 Self-encoding sensor with microspheres 有权
标题翻译：具有微球的自编码传感器
公开(公告)号：US08030094B2
公开(公告)日：2011-10-04
申请号：US12834422
申请日：2010-07-12
申请人： David R. Walt , Todd A. Dickinson
发明人： David R. Walt , Todd A. Dickinson
IPC分类号： G01N33/543 , G01N21/00 , G01N21/75
CPC分类号： C12Q1/682 , B01J2219/00317 , B01J2219/0045 , B01J2219/00466 , B01J2219/005 , B01J2219/00524 , B01J2219/00585 , B01J2219/00648 , B01J2219/00659 , C12Q1/6834 , C40B60/14 , G01N21/6428 , G01N21/6452 , G01N21/7703 , G01N33/0031 , G01N33/54313 , G01N2201/0846 , Y10T436/101666 , Y10T436/109163
摘要： A microsphere-based analytic chemistry system is disclosed in which self-encoding microspheres having distinct characteristic optical response signatures to specific target analytes may be mixed together while the ability is retained to identify the sensor type and location of each sensor in a random dispersion of large numbers of such sensors in a sensor array using an optically interrogatable encoding scheme. An optical fiber bundle sensor is also disclosed in which individual microsphere sensors are disposed in microwells at a distal end of the fiber bundle and are optically coupled to discrete fibers or groups of fibers within the bundle. The identities of the individual sensors in the array are self-encoded by exposing the array to a reference analyte while illuminating the array with excitation light energy. A single sensor array may carry thousands of discrete sensing elements whose combined signal provides for substantial improvements in sensor detection limits, response times and signal-to-noise ratios.
摘要翻译：公开了一种基于微球的分析化学系统，其中具有针对特定目标分析物的不同特征光学响应特征的自编码微球可以混合在一起，同时保留能力以识别大尺度随机色散中的每个传感器的传感器类型和位置使用光学可询问编码方案的传感器阵列中的这种传感器的数量。还公开了一种光纤束传感器，其中单个微球传感器设置在纤维束的远端处的微孔中，并且光束耦合到束内的离散纤维或纤维组。阵列中各个传感器的身份通过将阵列暴露于参考分析物而进行自编码，同时用激发光能照射阵列。单个传感器阵列可携带数千个离散感测元件，其组合信号提供传感器检测限，响应时间和信噪比方面的实质性改进。

58. 发明申请

US20110217791A1 METHOD FOR QUANTIFYING OR DETECTING DNA 审中-公开
标题翻译：定量或检测DNA的方法
公开(公告)号：US20110217791A1
公开(公告)日：2011-09-08
申请号：US13060167
申请日：2009-08-18
申请人： Yoshitaka Tomigahara , Hideo Satoh , Hirokazu Tarui
发明人： Yoshitaka Tomigahara , Hideo Satoh , Hirokazu Tarui
IPC分类号： G01N33/53 , C07H1/00 , C07K1/13
CPC分类号： C12Q1/682 , C12Q1/6834 , G01N33/5308 , C12Q2537/164 , C12Q2537/149 , C12Q2525/117 , C12Q2565/518 , C12Q2563/131 , C12Q2525/161
摘要： The present invention relates to a method for quantifying or detecting DNA having a target DNA region, and so on.
摘要翻译：本发明涉及用于定量或检测具有目标DNA区域的DNA等的方法。

59. 发明申请

US20110207623A1 METHODS FOR DETECTION AND QUANTIFICATION OF ANALYTES IN COMPLEX MIXTURES 有权
公开(公告)号：US20110207623A1
公开(公告)日：2011-08-25
申请号：US13027493
申请日：2011-02-15
申请人： KRASSEN DIMITROV , DWAYNE L. DUNAWAY
发明人： KRASSEN DIMITROV , DWAYNE L. DUNAWAY
IPC分类号： C40B30/04 , C40B40/06 , C40B50/00 , C07H21/04
CPC分类号： C12Q1/6888 , C12Q1/6816 , C12Q1/682 , C12Q1/6876 , C12Q2565/102 , C12Q2537/143 , C12Q2525/161 , C12Q2565/1025 , C12Q2563/179 , C12Q2525/151 , C12Q2525/101
摘要： The invention provides a diverse population of uniquely labeled probes, containing about thirty or more target specific nucleic acid probes each attached to a unique label bound to a nucleic acid. Also provided is a method of producing a population of uniquely labeled nucleic acid probes. The method consists of (a) synthesizing a population of target specific nucleic acid probes each having a different specifier; (b) synthesizing a corresponding population of anti-genedigits each having a unique label, the population having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) hybridizing the populations of target nucleic acid probes to the anti-genedigits, to produce a population in which each of the target specific probes is uniquely labeled. Also provided is a method of detecting a nucleic acid analyte. The method consists of (a) contacting a mixture of nucleic acid analytes under conditions sufficient for hybridization with a plurality of target specific nucleic acid probes each having a different specifier; (b) contacting the mixture under conditions sufficient for hybridization with a corresponding plurality of anti-genedigits each having a unique label, the plurality of anti-genedigits having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) uniquely detecting a hybridized complex between one or more analytes in the mixture, a target specific probe, and an anti-genedigit.

60. 发明授权

US07960104B2 Self-assembled single molecule arrays and uses thereof 有权
标题翻译：自组装单分子阵列及其用途
公开(公告)号：US07960104B2
公开(公告)日：2011-06-14
申请号：US11541225
申请日：2006-09-29
申请人： Radoje Drmanac , Matthew J. Callow , Brian K. Hauser , George Yeung
发明人： Radoje Drmanac , Matthew J. Callow , Brian K. Hauser , George Yeung
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34 , C12M1/36
CPC分类号： C12Q1/6837 , C12N15/1093 , C12Q1/682 , C12Q1/6869 , C12Q1/6874 , C12Q2531/125 , C12Q2525/313 , C12Q2525/151 , C12Q2521/313 , C12Q2565/513 , C12Q2521/307
摘要： The present invention provides methods of making and using self-assembled arrays of single polynucleotide molecules for carrying out a variety of large-scale genetic measurements, such as gene expression analysis, gene copy number assessment, and the like. Random arrays used in the invention are “self-assembled” in the sense that they are formed by deposition of polynucleotide molecules onto a surface where they become fixed at random locations. The polynucleotide molecules fixed on the surface are then identified by direct sequence determination of component nucleic acids, such as incorporated probe sequences, or by other decoding schemes. Such identification converts a random array of determinable polynucleotides, and their respective probes into an addressable array of probe sequences.
摘要翻译：本发明提供制造和使用单个多核苷酸分子的自组装阵列进行各种大规模遗传测量的方法，例如基因表达分析，基因拷贝数评估等。在本发明中使用的随机阵列是“自组装”的意思，即它们通过将多核苷酸分子沉积到其随机位置固定的表面上而形成。然后通过直接序列测定组分核酸，例如并入的探针序列或其他解码方案来鉴定固定在表面上的多核苷酸分子。这种鉴定将随机阵列的可测定多核苷酸及其各自的探针转换成可寻址的探针序列阵列。

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