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1. 发明授权

US10139361B2 Proteolysis detection 有权
公开(公告)号：US10139361B2
公开(公告)日：2018-11-27
申请号：US13978702
申请日：2011-12-22
申请人： Krassen Dimitrov
发明人： Krassen Dimitrov
IPC分类号： G01N27/327 , G01N33/49 , G01N33/86
摘要： Disclosed are electrochemical methods, apparatus, systems and kits for the detection or monitoring of proteolysis of proteinaceous matrices such as fibrin clots. The methods, apparatus, systems and kits generally include the use of voltammetric techniques to measure the changes in current that result from diffusion of an electroactive species towards an electrode on proteolysis of a proteinaceous matrix.

2. 发明申请

US20090220978A1 METHODS FOR DETECTION AND QUANTIFICATION OF ANALYTES IN COMPLEX MIXTURES 审中-公开
标题翻译：用于复合混合物中分析物的检测和定量的方法
公开(公告)号：US20090220978A1
公开(公告)日：2009-09-03
申请号：US12324357
申请日：2008-11-26
申请人： Krassen Dimitrov
发明人： Krassen Dimitrov
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6888 , C12Q1/6816 , C12Q1/682 , C12Q1/6876 , C12Q2565/102 , C12Q2537/143 , C12Q2525/161 , C12Q2565/1025 , C12Q2563/179 , C12Q2525/151 , C12Q2525/101
摘要： The invention provides a diverse population of uniquely labeled probes, containing about thirty or more target specific nucleic acid probes each attached to a unique label bound to a nucleic acid. Also provided is a method of producing a population of uniquely labeled nucleic acid probes. The method consists of (a) synthesizing a population of target specific nucleic acid probes each having a different specifier; (b) synthesizing a corresponding population of anti-genedigits each having a unique label, the population having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) hybridizing the populations of target nucleic acid probes to the anti-genedigits, to produce a population in which each of the target specific probes is uniquely labeled. Also provided is a method of detecting a nucleic acid analyte. The method consists of (a) contacting a mixture of nucleic acid analytes under conditions sufficient for hybridization with a plurality of target specific nucleic acid probes each having a different specifier; (b) contacting the mixture under conditions sufficient for hybridization with a corresponding plurality of anti-genedigits each having a unique label, the plurality of anti-genedigits having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) uniquely detecting a hybridized complex between one or more analytes in the mixture, a target specific probe, and an anti-genedigit.
摘要翻译：本发明提供了多种独特标记的探针，其含有约30个或更多个目标特异性核酸探针，其各自附着于与核酸结合的唯一标记。还提供了生产独特标记的核酸探针群体的方法。该方法包括（a）合成具有不同说明符的目标特异性核酸探针群; （b）合成各自具有独特标记的相应群体的抗Genieigits，所述群体具有足以独特地与所述说明者内的Genesigits杂交的多样性，以及（c）将靶核酸探针的群体与抗Genieigits杂交，以产生其中每个靶特异性探针被唯一标记的群体。还提供了检测核酸分析物的方法。该方法包括（a）在足以与多个具有不同说明符的靶特异性核酸探针杂交的条件下使核酸分析物的混合物接触; （b）在足以与各自具有唯一标记的相应多个抗基因组杂交的条件下接触混合物，所述多个抗Genetigits具有足以独特地与指定剂内的Genesigits杂交的分集，以及（c）唯一检测混合物中一种或多种分析物之间的杂交复合物，靶标特异性探针和抗Genetigigit。

3. 发明申请

US20070166708A1 Methods for detection and quantification of analytes in complex mixtures 有权
标题翻译：复杂混合物中分析物的检测和定量方法
公开(公告)号：US20070166708A1
公开(公告)日：2007-07-19
申请号：US10542458
申请日：2002-07-03
申请人： Krassen Dimitrov , Dwayne Dunaway
发明人： Krassen Dimitrov , Dwayne Dunaway
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6888 , C12Q1/6816 , C12Q1/682 , C12Q1/6876 , C12Q2565/102 , C12Q2537/143 , C12Q2525/161 , C12Q2565/1025 , C12Q2563/179 , C12Q2525/151 , C12Q2525/101
摘要： The invention provides a diverse population of uniquely labeled probes, containing about thirty or more target specific nucleic acid probes each attached to a unique label bound to a nucleic acid. Also provided is a method of producing a population of uniquely labeled nucleic acid probes. The method consists of (a) synthesizing a population of target specific nucleic acid probes each having a different specifier; (b) synthesizing a corresponding population of anti-genedigits each having a unique label, the population having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) hybridizing the populations of target nucleic acid probes to the anti-genedigits, to produce a population in which each of the target specific probes is uniquely labeled. Also provided is a method of detecting a nucleic acid analyte. The method consists of (a) contacting a mixture of nucleic acid analytes under conditions sufficient for hybridization with a plurality of target specific nucleic acid probes each having a different specifier; (b) contacting the mixture under conditions sufficient for hybridization with a corresponding plurality of anti-genedigits each having a unique label, the plurality of anti-genedigits having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) uniquely detecting a hybridized complex between one or more analytes in the mixture, a target specific probe, and an anti-genedigit.
摘要翻译：本发明提供了多种独特标记的探针，其含有约30个或更多个目标特异性核酸探针，其各自附着于与核酸结合的唯一标记。还提供了生产独特标记的核酸探针群体的方法。该方法包括（a）合成具有不同说明符的目标特异性核酸探针群; （b）合成各自具有独特标记的相应群体的抗Genieigits，所述群体具有足以独特地与所述说明者内的Genesigits杂交的多样性，以及（c）将靶核酸探针的群体与抗Genieigits杂交，以产生其中每个靶特异性探针被唯一标记的群体。还提供了检测核酸分析物的方法。该方法包括（a）在足以与多个具有不同说明符的靶特异性核酸探针杂交的条件下使核酸分析物的混合物接触; （b）在足以与各自具有唯一标记的相应多个抗基因组杂交的条件下接触混合物，所述多个抗Genetigits具有足以独特地与指定剂内的Genesigits杂交的分集，以及（c）唯一检测混合物中一种或多种分析物之间的杂交复合物，靶标特异性探针和抗Genetigigit。

4. 发明申请

US20140038218A1 PROTEOLYSIS DETECTION 审中-公开
标题翻译：防腐检测
公开(公告)号：US20140038218A1
公开(公告)日：2014-02-06
申请号：US13978702
申请日：2011-12-22
申请人： Krassen Dimitrov
发明人： Krassen Dimitrov
IPC分类号： G01N27/327
CPC分类号： G01N27/3275 , G01N33/4905 , G01N33/86
摘要： Disclosed are electrochemical methods, apparatus, systems and kits for the detection or monitoring of proteolysis of proteinaceous matrices such as fibrin clots. The methods, apparatus, systems and kits generally include the use of voltammetric techniques to measure the changes in current that result from diffusion of an electroactive species towards an electrode on proteolysis of a proteinaceous matrix.
摘要翻译：公开了用于检测或监测蛋白质基质如纤维蛋白凝块的蛋白水解的电化学方法，装置，系统和试剂盒。方法，装置，系统和试剂盒通常包括使用伏安技术来测量电活性物质向蛋白质基质的蛋白水解扩散所导致的电流变化。

5. 发明授权

US07964347B2 Labels for electronic detection of individual molecules and methods for their detection 有权
标题翻译：单个分子的电子检测标签及其检测方法
公开(公告)号：US07964347B2
公开(公告)日：2011-06-21
申请号：US11762003
申请日：2007-06-12
申请人： Krassen Dimitrov
发明人： Krassen Dimitrov
IPC分类号： C12Q1/68
CPC分类号： G01N33/54326 , C12Q1/6825 , G01N33/5438 , G01N33/587 , C12Q2565/102 , C12Q2563/113
摘要： The invention provides labels for electronic detection of individual molecules. The labels are comprised of elements with different electrical properties that affect the electric current flowing through a nanoelectrode. The labels are of polymeric or filamentous structure where the elements are arranged linearly along their length. The arrangement of the elements is predetermined and combinatorial, so that a high diversity of labels can be generated in a manner that resembles barcoding on a nanoscale level. Methods for the synthesis of said barcode labels and for the binding of the barcode labels to individual molecules, their movement past a nanoelectrode, and their detection are also provided.
摘要翻译：本发明提供用于单个分子的电子检测的标签。标签由具有不同电特性的元件组成，这些元件影响流过纳米电极的电流。标签是聚合物或丝状结构，其中元件沿其长度线性排列。元件的布置是预定的和组合的，使得可以以类似于纳米尺度级别的条形码的方式产生高分辨率的标签。还提供了用于合成所述条形码标签和将条形码标签与各个分子结合的方法，其经过纳米电极的运动及其检测方法。

6. 发明申请

US20110003715A1 METHODS FOR DETECTION AND QUANTIFICATION OF ANALYTES IN COMPLEX MIXTURES 有权
标题翻译：用于复合混合物中分析物的检测和定量的方法
公开(公告)号：US20110003715A1
公开(公告)日：2011-01-06
申请号：US12819101
申请日：2010-06-18
申请人： Krassen Dimitrov
发明人： Krassen Dimitrov
IPC分类号： C40B40/06
CPC分类号： C12Q1/6888 , C12Q1/6816 , C12Q1/682 , C12Q1/6876 , C12Q2565/102 , C12Q2537/143 , C12Q2525/161 , C12Q2565/1025 , C12Q2563/179 , C12Q2525/151 , C12Q2525/101
摘要： The invention provides a diverse population of uniquely labeled probes, containing about thirty or more target specific nucleic acid probes each attached to a unique label bound to a nucleic acid. Also provided is a method of producing a population of uniquely labeled nucleic acid probes. The method consists of (a) synthesizing a population of target specific nucleic acid probes each having a different specifier; (b) synthesizing a corresponding population of anti-genedigits each having a unique label, the population having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) hybridizing the populations of target nucleic acid probes to the anti-genedigits, to produce a population in which each of the target specific probes is uniquely labeled. Also provided is a method of detecting a nucleic acid analyte. The method consists of (a) contacting a mixture of nucleic acid analytes under conditions sufficient for hybridization with a plurality of target specific nucleic acid probes each having a different specifier; (b) contacting the mixture under conditions sufficient for hybridization with a corresponding plurality of anti-genedigits each having a unique label, the plurality of anti-genedigits having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) uniquely detecting a hybridized complex between one or more analytes in the mixture, a target specific probe, and an anti-genedigit.
摘要翻译：本发明提供了多种独特标记的探针，其含有约30个或更多个目标特异性核酸探针，其各自附着于与核酸结合的唯一标记。还提供了生产独特标记的核酸探针群体的方法。该方法包括（a）合成具有不同说明符的目标特异性核酸探针群; （b）合成各自具有独特标记的相应群体的抗Genieigits，所述群体具有足以独特地与所述说明者内的Genesigits杂交的多样性，以及（c）将靶核酸探针的群体与抗Genieigits杂交，以产生其中每个靶特异性探针被唯一标记的群体。还提供了检测核酸分析物的方法。该方法包括（a）在足以与多个具有不同说明符的靶特异性核酸探针杂交的条件下使核酸分析物的混合物接触; （b）在足以与各自具有唯一标记的相应多个抗基因组杂交的条件下接触混合物，所述多个抗Genetigits具有足以独特地与指定剂内的Genesigits杂交的分集，以及（c）唯一检测混合物中一种或多种分析物之间的杂交复合物，靶标特异性探针和抗Genetigigit。

7. 发明授权

US10923744B2 Electrochemical systems for direct generation of electricity and heat pumping 有权
公开(公告)号：US10923744B2
公开(公告)日：2021-02-16
申请号：US16063700
申请日：2016-01-07
申请人： KD INNOVATION LTD. , Krassen Dimitrov , Angel Giancarlo Miranda , Pei-Hsuan Chiang
发明人： Krassen Dimitrov , Angel Giancarlo Miranda , Pei-Hsuan Chiang
IPC分类号： H01M8/04007 , H01M8/18 , H01M14/00 , H01M2/16 , H01M8/1004
摘要： An electrochemical system that can serve as a heat engine or heat pump is disclosed. The electrochemical system comprises a first electrode assembly, a second electrode assembly, a first electrolyte and a second electrolyte. The first electrode assembly, configured to operate at a first temperature, includes a first electrode set and a second electrode set. The second electrode assembly, configured to operate at a second temperature different from the first temperature, includes a first electrode set and a second electrode set. The first electrolyte is configured to circulate between the first electrode set of the first electrode assembly and the first electrode set of the second electrode assembly. The second electrolyte is configured to circulate between the second electrode set of the first electrode assembly and the second electrode set of the second electrode assembly.

8. 发明申请

US20140134659A1 SYSTEM AND METHOD FOR DETECTING AND MONITORING PROTEOLYSIS OF PROTEIN MATRICES 有权
标题翻译：用于检测和监测蛋白质基质的蛋白质的系统和方法
公开(公告)号：US20140134659A1
公开(公告)日：2014-05-15
申请号：US14145986
申请日：2014-01-01
申请人： Krassen DIMITROV
发明人： Krassen DIMITROV
IPC分类号： C12Q1/37
CPC分类号： G01N27/3277 , A61B5/053 , C12Q1/005 , C12Q1/56 , G01N27/026 , G01N33/48707 , G01N33/49
摘要： Apparatus and methods are provided for detecting and monitoring proteolysis of protein matrices such as fibrin clots, extracellular matrix and collagen matrix. The apparatus and methods involve in general the measurement of diffusion-limited electrochemical currents generated by an electroactive species or elactomer in a synthetic protein matrix. The apparatus and methods are applied in various embodiments to detect and monitor the proteolytic activities of a sample, and more specifically fibrinolytic activities and collagenase activities of a sample. The apparatus and methods are utilized generally in the monitoring and diagnostics of cardiovascular, cerebralvascular, and oncology conditions.
摘要翻译：提供了用于检测和监测蛋白质基质如纤维蛋白凝块，细胞外基质和胶原基质的蛋白水解的装置和方法。该装置和方法通常涉及由合成蛋白质基质中的电活性物质或活化剂产生的扩散限制电化学电流的测量。该装置和方法应用于各种实施方案中以检测和监测样品的蛋白水解活性，更具体地说是纤维蛋白溶解活性和样品的胶原酶活性。该装置和方法通常用于监测和诊断心血管，脑血管和肿瘤病症。

9. 发明申请

US20140014506A1 ANALYTE TRANSPORT 审中-公开
标题翻译：分析运输
公开(公告)号：US20140014506A1
公开(公告)日：2014-01-16
申请号：US13993248
申请日：2011-12-14
申请人： Krassen Dimitrov
发明人： Krassen Dimitrov
IPC分类号： G01N27/30
CPC分类号： G01N27/30 , G01N27/745
摘要： Apparatus for sensing an analyte, the analyte being bound to at least one magnetic particle to form a labelled analyte, and wherein the apparatus includes a cavity having a sensor therein, wherein in use the cavity contains a fluid and at least one labelled analyte and a magnet system for generating a magnetic field having a field direction substantially orthogonal to at least part of the cavity to orientate the at least one labelled analyte in the cavity and a field gradient along at least part of the cavity to thereby urge the at least one labelled analyte along the cavity towards the sensor.
摘要翻译：用于感测分析物的装置，所述分析物结合至少一个磁性颗粒以形成标记的分析物，并且其中所述装置包括其中具有传感器的空腔，其中在使用中所述空腔包含流体和至少一个标记的分析物和磁体系统，用于产生磁场，其磁场方向基本上与所述空腔的至少一部分正交，以使所述腔中的所述至少一个标记的分析物定向，并且沿着所述腔的至少一部分的场梯度，从而促使所述至少一个标记的分析物沿着空腔朝向传感器。

10. 发明授权

US08492094B2 Methods for detection and quantification of analytes in complex mixtures 有权
标题翻译：复杂混合物中分析物的检测和定量方法
公开(公告)号：US08492094B2
公开(公告)日：2013-07-23
申请号：US13027493
申请日：2011-02-15
申请人： Krassen Dimitrov , Dwayne L. Dunaway
发明人： Krassen Dimitrov , Dwayne L. Dunaway
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6888 , C12Q1/6816 , C12Q1/682 , C12Q1/6876 , C12Q2565/102 , C12Q2537/143 , C12Q2525/161 , C12Q2565/1025 , C12Q2563/179 , C12Q2525/151 , C12Q2525/101
摘要： The invention provides a method of detecting a nucleic acid analyte. The method consists of (a) contacting a mixture of nucleic acid analytes under conditions sufficient for hybridization with a plurality of target specific nucleic acid probes each having a different specifier; (b) contacting the mixture under conditions sufficient for hybridization with a corresponding plurality of antigenedigits each having a unique label, the plurality of anti-genedigits having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) uniquely detecting a hybridized complex between one or more analytes in the mixture, a target specific probe, and an anti-genedigit.
摘要翻译：本发明提供了一种检测核酸分析物的方法。该方法包括（a）在足以与多个具有不同说明符的靶特异性核酸探针杂交的条件下使核酸分析物的混合物接触; （b）在足以与各自具有唯一标记的相应多个抗原多肽杂交的条件下使混合物接触，所述多个抗Genetigits具有足以独特地与指定剂内的genesigits杂交的多样性，以及（c）唯一地检测杂交的混合物中一种或多种分析物之间的复合物，目标特异性探针和抗Genetigigit。

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