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    • 54. 发明授权
    • Deparaffinization compositions and methods for their use
    • 脱蜡组合物及其使用方法
    • US06632598B1
    • 2003-10-14
    • US08212175
    • 1994-03-11
    • Guangrong ZhangCheng-Zhi YuSheng-Hui SuKrishan L. KalraDing Zhou
    • Guangrong ZhangCheng-Zhi YuSheng-Hui SuKrishan L. KalraDing Zhou
    • C12Q100
    • C11D1/143C11D1/62C11D1/72C11D3/187C11D3/188C11D3/201C11D3/2044G01N1/30G01N1/36Y10S435/975
    • Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual dewaxing composition. Also provided is a kit for dewaxing a wax-embedded specimen, which comprises a dewaxing composition and can further comprise a second composition of (1) an immunostaining reagent or (2) an aqueous wash solution comprising a detergent for removing residual dewaxing solution.
    • 提供组合物和方法,用于在组织化学分析前对蜡嵌入的生物样品进行脱蜡。 所提供的组合物和方法可以在用于组织化学或其他诊断分析的准备期间有效地从标本中去除蜡或改进的蜡基嵌入材料,特别是基于石蜡的包埋材料,同时最小化对使用者的危害,实现与自动使用的兼容性并保持与下游的兼容性 组织化学分析,特别是免疫染色。 本发明的组合物包含石蜡溶解性有机溶剂,极性有机溶剂和表面活性剂。 组合物还可以包含水。 该方法包括使蜡包埋的样品与脱蜡组合物接触,以在组织化学分析之前溶解浸渍样品的蜡。 该方法可以包括在用包含洗涤剂的水性洗涤组合物脱蜡之后立即洗涤脱蜡样品以除去残余脱蜡组合物的步骤。 还提供了一种用于脱蜡蜡包埋样品的试剂盒,其包含脱蜡组合物,并且还可以包含第二组合物(1)免疫染色试剂或(2)包含用于除去残余脱蜡溶液的洗涤剂的水性洗涤溶液。 / PTEXT>