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51. 发明授权

US06762062B2 Method of determining cholesterol and sensor applicable to the same 失效
标题翻译：测定胆固醇和传感器的方法适用于此
公开(公告)号：US06762062B2
公开(公告)日：2004-07-13
申请号：US09961930
申请日：2001-09-24
申请人： Motokazu Watanabe , Toshihiko Yoshioka , Shiro Nankai
发明人： Motokazu Watanabe , Toshihiko Yoshioka , Shiro Nankai
IPC分类号： G01N3353
CPC分类号： G01N27/3272 , G01N33/92 , G01N2800/044 , G01N2800/52 , Y10S435/803 , Y10S435/814 , Y10S435/817 , Y10S435/962 , Y10S435/973 , Y10S436/806 , Y10S436/815 , Y10S436/824 , Y10S436/825 , Y10T436/25375 , Y10T436/255
摘要： The present disclosure relates to a method for determining cholesterol in low density lipoprotein comprising the steps of (a) measuring total cholesterol level in a sample containing at least high density lipoprotein, low density lipoprotein, very low density lipoprotein and chylomicron, and (b) measuring cholesterol levels in the high density lipoprotein, very low density lipoprotein and chylomicron in the sample, wherein the cholesterol level in the low density lipoprotein is determined by subtracting a value obtained in the step (b) from a value obtained in the step (a). The present invention enables concurrent determination of cholesterol level in low density lipoprotein and total cholesterol level, facilitating acquisition of two types of biological information at a time.
摘要翻译：本公开涉及一种用于测定低密度脂蛋白胆固醇的方法，包括以下步骤：（a）测量含有至少高密度脂蛋白，低密度脂蛋白，极低密度脂蛋白和乳糜微粒的样品中的总胆固醇水平，和（b）测量样品中高密度脂蛋白，极低密度脂蛋白和乳糜微粒中的胆固醇水平，其中通过从步骤（a）中获得的值减去步骤（b）中获得的值来确定低密度脂蛋白中的胆固醇水平）。本发明能够同时测定低密度脂蛋白和总胆固醇水平的胆固醇水平，便于一次获取两种生物信息。

52. 发明授权

US06740215B1 Biosensor 失效
标题翻译：生物传感器
公开(公告)号：US06740215B1
公开(公告)日：2004-05-25
申请号：US09959571
申请日：2001-10-30
申请人： Takahiro Nakaminami , Motokazu Watanabe , Shin Ikeda , Shiro Nankai
发明人： Takahiro Nakaminami , Motokazu Watanabe , Shin Ikeda , Shiro Nankai
IPC分类号： G01N27327
CPC分类号： C12Q1/005
摘要： A biosensor comprising an electrically insulating base plate, an electrode system containing a working electrode and a counter electrode disposed on the base plate, and a reagent system comprising at least an oxidoreductase, a hydrophilic polymer and an electron mediator, wherein the reagent system further comprises a substance having a function to convert an organic product generated by direct reaction of a substrate to be measured with the oxidoreductase to another compound.
摘要翻译：一种生物传感器，包括电绝缘基板，包含工作电极的电极系统和设置在基板上的对电极，以及至少包含氧化还原酶，亲水性聚合物和电子介体的试剂系统，其中所述试剂系统还包括具有将待测基质与氧化还原酶直接反应产生的有机产物转化为其他化合物的功能的物质。

53. 发明授权

US06719887B2 Biosensor 有权
标题翻译：生物传感器
公开(公告)号：US06719887B2
公开(公告)日：2004-04-13
申请号：US10221990
申请日：2002-09-17
申请人： Miwa Hasegawa , Motokazu Watanabe , Tomohiro Yamamoto , Shin Ikeda , Shiro Nankai
发明人： Miwa Hasegawa , Motokazu Watanabe , Tomohiro Yamamoto , Shin Ikeda , Shiro Nankai
IPC分类号： G01N27327
CPC分类号： G01N33/92 , C12Q1/005 , G01N33/5438
摘要： A biosensor includes: an insulating base plate; an electrode system which is provided on the base plate and has a measurement electrode and a counter electrode; a reaction layer including at least oxidoreductase and an electron mediator; a sample solution supply pathway which includes the electrode system and the reaction layer and has an air aperture on the termination side thereof; a sample supply portion; and a filter which is disposed between the sample solution supply pathway and the sample supply portion and filters hemocytes, where plasma with the hemocytes therein filtered with the filter is sucked into the sample solution supply pathway due to capillarity, the central part of a secondary side portion of the filter is protruded into the sample solution supply pathway more than both the right and left ends thereof.
摘要翻译：生物传感器包括：绝缘基板; 电极系统，设置在基板上，具有测量电极和对电极; 至少包含氧化还原酶和电子介体的反应层; 样品溶液供给路径，其包括电极系统和反应层，并且在其终端侧具有空气孔; 样品供应部分; 以及过滤器，其设置在样品溶液供给路径和样品供给部之间，并且过滤血细胞，其中由过滤器过滤的血细胞的血浆由于毛细作用而被吸入样品溶液供给通道，次级侧的中心部分过滤器的一部分比其右端和左端都突出到样品溶液供给路径中。

54. 发明授权

US06699382B2 Method for analyzing a biological sample 有权
标题翻译：分析生物样品的方法
公开(公告)号：US06699382B2
公开(公告)日：2004-03-02
申请号：US10235451
申请日：2002-09-04
申请人： Toshihiko Yoshioka , Shin Ikeda , Motokazu Watanabe , Shiro Nankai
发明人： Toshihiko Yoshioka , Shin Ikeda , Motokazu Watanabe , Shiro Nankai
IPC分类号： G01N27327
CPC分类号： C12Q1/001
摘要： A biosensor enabling accurate measurement of the concentration of a substrate contained in a trace amount of a sample solution is disclosed. The electrochemical analysis element in accordance with the present invention which should be included in the biosensor comprises a sensor body having a hollow space which space is open at its both ends, an electrode system having a working electrode and a counter electrode, a reagent segment containing an enzyme, wherein the electrode system and the reagent segment are disposed on an inner wall of the hollow space.
摘要翻译：公开了能够精确测量微量样品溶液中所含的基板的浓度的生物传感器。应包括在生物传感器中的根据本发明的电化学分析元件包括具有中空空间的传感器本体，其两端开放空间，具有工作电极和对电极的电极系统，含有酶，其中电极系统和试剂段设置在中空空间的内壁上。

55. 发明授权

US06471839B1 Biosensor 失效
标题翻译：生物传感器
公开(公告)号：US06471839B1
公开(公告)日：2002-10-29
申请号：US09572797
申请日：2000-05-17
申请人： Tomohiro Yamamoto , Toshihiko Yoshioka , Shiro Nankai
发明人： Tomohiro Yamamoto , Toshihiko Yoshioka , Shiro Nankai
IPC分类号： G01N27327
CPC分类号： C12Q1/004 , C12Q1/005
摘要： The present invention provides a biosensor which enables quick measurement and is excellent in storage characteristic. This biosensor comprises an electrically insulating base plate on which an electrode system having at least a measuring electrode and a counter electrode is formed, a cover member which is integrated to the base plate so as to form a sample solution supply path for supplying a sample solution to the electrode system between the cover member and the base plate, and a carrier composed of fiber supporting a reagent containing at least an oxidoreductase, and the carrier is placed in the sample solution supply path. Preferably, the carrier is constituted at least of two carrier pieces and each carrier piece supports a different reagent.
摘要翻译：本发明提供一种生物传感器，其能够快速测量并且具有优异的储存特性。该生物传感器包括电绝缘基板，其上形成有至少具有测量电极和对电极的电极系统，盖构件，其与基板一体地形成用于提供样品溶液的样品溶液供应路径涉及覆盖构件和基板之间的电极系统，以及由支撑含有至少一种氧化还原酶的试剂的纤维构成的载体，载体被放置在样品溶液供给路径中。优选地，载体至少由两个载体片构成，并且每个载体片支撑不同的试剂。

56. 再颁专利

USRE37002E Lipid-dispersed solution, production thereof and use in determining lipid levels 失效
标题翻译：脂质分散溶液，其生产和用于确定脂质水平
公开(公告)号：USRE37002E
公开(公告)日：2000-12-26
申请号：US327019
申请日：1999-06-07
申请人： Tomohiro Yamamoto , Toshihiko Yoshioka , Shiro Nankai
发明人： Tomohiro Yamamoto , Toshihiko Yoshioka , Shiro Nankai
IPC分类号： G01N33/92 , G01N33/96 , B01J13/00 , C12Q1/60
CPC分类号： G01N33/96 , G01N33/92 , G01N2800/044 , Y10S516/928 , Y10T436/104165
摘要： .[.There is disclosed an aqueous dispersed solution as a standard solution for determining lipid levels, having a dispersion form and a particle size, which are similar to those of serum lipids. A lipid-dispersed solution is obtained by evaporating an organic solvent from a mixture prepared by adding cholesterol, a phospholipid, a bile acid or bile acid salt, and a neutral lipid and/or a cholesterol ester in the organic solvent, swelling the resultant thin-film like mixture with water or buffer heated at a temperature higher than a phase transition temperature of the lecithin, and then dispersing the solution by a physical shear force. There can be easily obtained a solution having a cholesterol concentration of about less than 1,000 mg/dl, which is extremely stable and can be stored for a long period of time..]. .Iadd.There is disclosed an aqueous dispersed solution as a standard solution for determining lipid levels, having a dispersion form and a particle size, which are similar to those of serum lipids. A lipid-dispersed solution is obtained by evaporating an organic solvent from a mixture prepared by adding cholesterol, a phospholipid, a bile acid or bile acid salt, and a neutral lipid and/or a cholesterol ester in the organic solvent, swelling the resultant thin-film like mixture with water or buffer heated at a temperature higher than a phase transition temperature of the lecithin, and then dispersing the solution by a physical shear force. These can be easily obtained a solution having a cholesterol concentration of about less than 1,000 mg/dl, which is extremely stable and can be stored for a long period of time. The lipid dispersed solution is used as a standard solution in calibrating a device for performing enzymatic lipid level determination, and the calibrated device is used to determine the lipid level (e.g. cholesterol) in a solution.
摘要翻译：公开了水分散溶液作为用于测定脂质水平的标准溶液，具有与血清脂质相似的分散体形式和粒径。通过将有机溶剂从通过在有机溶剂中加入胆固醇，磷脂，胆汁酸或胆汁酸盐和中性脂质和/或胆固醇酯制备的混合物蒸发而获得脂质分散溶液，将所得薄片在与高于卵磷脂相变温度的温度下加热的水或缓冲液混合，然后通过物理剪切力分散溶液。可以容易地获得胆固醇浓度大约小于1,000mg / dl的溶液，其非常稳定并且可以长期储存。]公开了作为确定脂质的标准溶液的水性分散溶液水平，具有与血清脂质相似的分散体形式和粒径。通过将有机溶剂从通过在有机溶剂中加入胆固醇，磷脂，胆汁酸或胆汁酸盐和中性脂质和/或胆固醇酯制备的混合物蒸发而获得脂质分散溶液，将所得薄片在与高于卵磷脂相变温度的温度下加热的水或缓冲液混合，然后通过物理剪切力分散溶液。这些可以容易地获得胆固醇浓度大约小于1,000mg / dl的溶液，其非常稳定并且可以长期储存。脂质分散溶液用作校准用于进行酶脂质水平测定的装置的标准溶液，并且校准的装置用于测定溶液中的脂质水平（例如胆固醇）。

57. 发明授权

US5985130A Method for quantifying substrate 失效
标题翻译：定量底物的方法
公开(公告)号：US5985130A
公开(公告)日：1999-11-16
申请号：US834829
申请日：1997-04-03
申请人： Shin Ikeda , Toshihiko Yoshioka , Shiro Nankai
发明人： Shin Ikeda , Toshihiko Yoshioka , Shiro Nankai
IPC分类号： G01N27/327 , C12Q1/00 , G01N27/416 , G01N27/49 , G01N27/26
CPC分类号： C12Q1/001 , G01N27/3273
摘要： The present invention provides a method for quantifying a substrate having improved measurement accuracy and measurement sensitivity. According to the method for quantifying the substrate of the present invention, a constant voltage is applied to an electrode system, between a working electrode and a counter electrode, via a resistor connected in series with the electrode system after placing a sample solution containing the substrate on the electrode system, so as to oxidize an electron acceptor, which has been reduced by an enzyme reaction of the substrate, thereby determining a concentration of the substrate included in the sample solution based on an oxidizing current flowing in the course of oxidation.
摘要翻译：本发明提供了一种用于量化具有改进的测量精度和测量灵敏度的衬底的方法。根据本发明的基板的定量化方法，在放置含有基板的样品溶液之后，通过与电极系统串联连接的电阻器将恒定电压施加到工作电极与对电极之间的电极系统在电极系统上，以通过基板的酶反应而还原的电子受体氧化，从而基于在氧化过程中流动的氧化电流来确定样品溶液中包含的底物的浓度。

58. 发明授权

US5650062A Biosensor, and a method and a device for quantifying a substrate in a sample liquid using the same 失效
标题翻译：生物传感器，以及使用其进行样品液体中的基板的定量的方法和装置
公开(公告)号：US5650062A
公开(公告)日：1997-07-22
申请号：US526557
申请日：1995-09-12
申请人： Shin Ikeda , Toshihiko Yoshioka , Shiro Nankai , Haruhiro Tsutsumi , Hideyuki Baba , Yoshinobu Tokuno , Syoji Miyazaki
发明人： Shin Ikeda , Toshihiko Yoshioka , Shiro Nankai , Haruhiro Tsutsumi , Hideyuki Baba , Yoshinobu Tokuno , Syoji Miyazaki
IPC分类号： C12Q1/00 , G01N27/26
CPC分类号： C12Q1/001 , Y10S435/817
摘要： The biosensor of this invention can quantify a substrate in a sample liquid by electrochemically measuring the amount of an electron acceptor that has been reduced by electrons generated in a reaction between the substrate and an oxidoreductase. The biosensor has an electrically insulating substrate and an electrode system formed on the substrate including a working electrode, a counter electrode and a third electrode used for detecting a liquid junction. The third electrode can be used merely for detecting a liquid junction, or can be used as both a reference electrode and a liquid junction detecting electrode.
摘要翻译：本发明的生物传感器可以通过电化学测量由底物和氧化还原酶之间的反应中产生的电子而减少的电子受体的量来量化样品液体中的底物。生物传感器具有电绝缘基板和形成在基板上的电极系统，该基板包括工作电极，对电极和用于检测液体接合点的第三电极。第三电极可以仅用于检测液体接点，也可以用作参考电极和液体接点检测电极。

59. 发明授权

US5409583A Method for measuring concentrations of substrates in a sample liquid by using a biosensor 失效
标题翻译：通过使用生物传感器测量样品液体中底物浓度的方法
公开(公告)号：US5409583A
公开(公告)日：1995-04-25
申请号：US128852
申请日：1993-09-28
申请人： Toshihiko Yoshioka , Satoko Fujisawa , Mariko Miyahara , Shiro Nankai
发明人： Toshihiko Yoshioka , Satoko Fujisawa , Mariko Miyahara , Shiro Nankai
IPC分类号： G01N27/28 , C12Q1/00 , G01N27/327 , G01N27/416 , G01N27/26
CPC分类号： C12Q1/004
摘要： The present invention provides a method for measuring concentrations of the first and second substrates in a sample liquid by using a biosensor. The biosensor has an electrically insulating substrate, an electrode system, and a reaction layer including a first enzyme, a second enzyme and an electron acceptor. The method includes the steps of measuring a response current value I.sub.1 of a predetermined period of time T.sub.1 after supplying the sample liquid including the first and second substrates to the biosensor; measuring a response current value I.sub.2 of a predetermined period of time T.sub.2 after the supply of the sample liquid; obtaining the concentration C.sub.1 of the first substrate corresponding to the current value I.sub.1 based on the previously determined relationship between the concentration of the first substrate and the response current value of the predetermined period of time T.sub.1 after the supply; obtaining a response current value I.sub.1 ' corresponding to the concentration C.sub.1 of the first substrate in the sample liquid based on the previously determined relationship between the concentration of the first substrate and the response current value of the predetermined period of time T.sub.2 after the supply; and obtaining the concentration C.sub.2 of the second substrate in the sample liquid corresponding to the current value calculated by subtracting the current value I.sub.1 ' from the current value I.sub.2 based on the previously determined relationship between the concentration of the second substrate and the response current value of the predetermined period of time T.sub.2 after the supply.
摘要翻译：本发明提供一种通过使用生物传感器测量样品液体中的第一和第二基板的浓度的方法。生物传感器具有电绝缘基板，电极系统和包括第一酶，第二酶和电子受体的反应层。该方法包括以下步骤：将包括第一和第二基板的样品液体供应到生物传感器之后，测量预定时间段T1的响应电流值I1; 在供给样品液体之后测量预定时间段T2的响应电流值I2; 基于先前确定的第一基板的浓度与供给后的预定时间段T1的响应电流值之间的关系，获得对应于电流值I1的第一基板的浓度C1; 基于先前确定的第一基板的浓度与供给后的预定时间段T2的响应电流值之间的关系，获得与样品液体中的第一基板的浓度C1相对应的响应电流值I1' 并且基于先前确定的第二基板的浓度与第二基板的响应电流值之间的关系，获得对应于通过从当前值I2减去电流值I1'而计算出的当前值的样品液体中的第二基板的浓度C2 在供给之后的预定时间段T2。

60. 再颁专利

USRE39390E1 Biosensor 失效
标题翻译：生物传感器
公开(公告)号：USRE39390E1
公开(公告)日：2006-11-14
申请号：US10924334
申请日：2002-05-17
申请人： Miwa Hasegawa , Tomohiro Yamamoto , Motokazu Watanabe , Takahiro Nakaminami , Shin Ikeda , Toshihiko Yoshioka , Shiro Nankai
发明人： Miwa Hasegawa , Tomohiro Yamamoto , Motokazu Watanabe , Takahiro Nakaminami , Shin Ikeda , Toshihiko Yoshioka , Shiro Nankai
IPC分类号： G01N27/327
摘要： There is provided a cholesterol sensor with high-accuracy and excellent response, whose object to be measured is whole blood, where plasma with which is obtained by filtering out hemocytes therein filtered in blood can rapidly reach an electrode system. In a biosensor where plasma with which is obtained by filtering out hemocytes therein filtered with by a filter is sucked into a sample solution supply pathway due to capillarity, there are formed: a first pressing part for holding a primary side portion of the filter from the bottom; a second pressing part for holding a secondary side portion of the filter from the top and the bottom; a third pressing part for holding the central portion of the filter from the top; and a void for surrounding the filter between the second pressing part and third pressing part.
摘要翻译：提供了具有高精度和优异反应的胆固醇传感器，其测量对象为全血，等离子体<？delete-start id =“DEL-S-00001”date =“20061114”？>与<？delete -end id =“DEL-S-00001”？> <？insert-start id =“INS-S-00001”date =“20061114”？>通过过滤出<？insert-end id =“INS- S-00001“？>血细胞<？delete-start id =”DEL-S-00002“date =”20061114“？>其中过滤的<？delete-end id =”DEL-S-00002“？> 在血液中开始id =“INS-S-00002”date =“20061114”？> <？insert-end id =“INS-S-00002”？>可以快速到达电极系统。在等离子体<？delete-start id =“DEL-S-00003”date =“20061114”？>与<？delete-end id =“DEL-S-00003”？> <？insert-start id = 通过滤出<？insert-end id =“INS-S-00003”？>血细胞<？delete-start id =“DEL-S-00004”获得的“INS-S-00003”date =“20061114”？ “date =”20061114“？>用<？delete-end id =”DEL-S-00004“？> <？insert-start id =”INS-S-00004“date =”20061114“？> ？insert-end id =“INS-S-00004”？>由于毛细作用，将过滤器吸入样品溶液供给路径，形成有：从底部保持过滤器的初级侧部的第一按压部，用于从顶部和底部保持过滤器的次级侧部分的第二按压部分; 用于从顶部保持过滤器的中心部分的第三按压部分; 以及用于围绕第二按压部和第三按压部之间的过滤器的空隙。

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