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51. 发明授权

US07741088B2 Immobilized microbial nitrilase for production of glycolic acid 有权
标题翻译：用于生产乙醇酸的固定化微生物腈水解酶
公开(公告)号：US07741088B2
公开(公告)日：2010-06-22
申请号：US11930744
申请日：2007-10-31
申请人： Robert DiCosimo , Arie Ben-Bassat
发明人： Robert DiCosimo , Arie Ben-Bassat
IPC分类号： C12N9/96 , C12N9/00 , C12N9/78 , C12P21/06 , C07H21/04
CPC分类号： C12N9/78 , C12N11/06 , C12N11/10 , C12P7/42 , Y02P20/588
摘要： The present invention is directed to a process for improving the specific activity of a dehydrated enzyme catalyst having nitrilase activity for hydrolysis of glycolonitrile to glycolic acid upon rehydration. In particular, a process is provided comprising pretreating an enzyme catalyst having nitrilase activity with glutaraldehyde, immobilizing the gutaraldehyde-preteated enzyme catalyst and chemically cross-linking the enzyme catalyst prior to dehydration. Upon rehydration, the enzyme catalyst exhibits improved specific nitrilase activity as compared to enzyme catalysts having nitrilase activity that are dehydrated and rehydrated without the processing described herein.
摘要翻译：本发明涉及一种提高具有腈水解酶活性的脱水酶催化剂的比活性的方法，所述脱水酶催化剂在补液时将乙醇腈水解成乙醇酸。特别地，提供了一种方法，其包括用戊二醛预处理具有腈水解酶活性的酶催化剂，在脱水之前固定化古巴醛预处理的酶催化剂和化学交联酶催化剂。在再水合时，与不进行本文所述处理的具有脱水和再水合的腈水解酶活性的酶催化剂相比，酶催化剂表现出提高的特异性腈水解酶活性。

52. 发明申请

US20100086535A1 STABILIZATION OF PERHYDROLASES 有权
标题翻译： PERHYDROLASES的稳定性
公开(公告)号：US20100086535A1
公开(公告)日：2010-04-08
申请号：US12572086
申请日：2009-10-01
申请人： Robert DiCosimo , Arie Ben-Bassat , Mark S. Payne , Raymond Richard Zolandz
发明人： Robert DiCosimo , Arie Ben-Bassat , Mark S. Payne , Raymond Richard Zolandz
IPC分类号： A01N63/00 , A01P1/00 , C11D3/386 , C11D3/39 , C12P7/40 , D06L3/02 , D06L1/00
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein is a method for stabilization of the perhydrolase activity of the CE-7 esterase in a formulation with a carboxylic acid ester that employs the addition of a buffering agent, substantially undissolved, to the mixture of the CE-7 esterase and the carboxylic acid ester. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.
摘要翻译：本文公开了一种在具有羧酸酯的制剂中稳定CE-7酯酶的过水解酶活性的方法，其使用向CE-7酯酶和羧酸的混合物中添加基本上未溶解的缓冲剂酯。此外，提供包含通过本文所述方法生产的过酸的消毒剂和衣物护理制剂。

53. 发明申请

US20100086534A1 STABILIZATION OF PERHYDROLASES 有权
标题翻译： PERHYDROLASES的稳定性
公开(公告)号：US20100086534A1
公开(公告)日：2010-04-08
申请号：US12572070
申请日：2009-10-01
申请人： Robert DiCosimo , Arie Ben-Bassat , Mark S. Payne , Raymond Richard Zolandz
发明人： Robert DiCosimo , Arie Ben-Bassat , Mark S. Payne , Raymond Richard Zolandz
IPC分类号： A01N63/00 , C12N9/96 , A01P1/00 , C11D3/386 , C12P7/40 , D06L3/02
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein are enzyme powders comprising a spray-dried formulation of at least one CE-7 esterase, at least one oligosaccharide excipient, and optionally at least one surfactant. Also disclosed herein is a process for producing peroxycarboxylic acids from carboxylic acid esters using the aforementioned enzyme powders. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.
摘要翻译：本文公开了包含至少一种CE-7酯酶，至少一种寡糖赋形剂和任选的至少一种表面活性剂的喷雾干燥制剂的酶粉末。本文还公开了使用上述酶粉末从羧酸酯制备过氧羧酸的方法。此外，提供包含通过本文所述方法生产的过酸的消毒剂和衣物护理制剂。

54. 发明申请

US20090111148A1 IMMOBILIZED MICROBIAL NITRILASE FOR PRODUCTION OF GLYCOLIC ACID 有权
标题翻译：用于生产甘油酸的稳定的微生物硝酸盐
公开(公告)号：US20090111148A1
公开(公告)日：2009-04-30
申请号：US11930744
申请日：2007-10-31
申请人： ROBERT DICOSIMO , Arie Ben-Bassat
发明人： ROBERT DICOSIMO , Arie Ben-Bassat
IPC分类号： C12P21/04 , C12N9/00
CPC分类号： C12N9/78 , C12N11/06 , C12N11/10 , C12P7/42 , Y02P20/588
摘要： The present invention is directed to a process for improving the specific activity of a dehydrated enzyme catalyst having nitrilase activity for hydrolysis of glycolonitrile to glycolic acid upon rehydration. In particular, a process is provided comprising pretreating an enzyme catalyst having nitrilase activity with glutaraldehyde, immobilizing the gutaraldehyde-preteated enzyme catalyst and chemically cross-linking the enzyme catalyst prior to dehydration. Upon rehydration, the enzyme catalyst exhibits improved specific nitrilase activity as compared to enzyme catalysts having nitrilase activity that are dehydrated and rehydrated without the processing described herein.
摘要翻译：本发明涉及一种提高具有腈水解酶活性的脱水酶催化剂的比活性的方法，所述脱水酶催化剂在补液时将乙醇腈水解成乙醇酸。特别地，提供了一种方法，其包括用戊二醛预处理具有腈水解酶活性的酶催化剂，在脱水之前固定化古巴醛预处理的酶催化剂和化学交联酶催化剂。在再水合时，与不进行本文所述处理的具有脱水和再水合的腈水解酶活性的酶催化剂相比，酶催化剂表现出提高的特异性腈水解酶活性。

55. 发明授权

US07229806B2 Microbial conversion of glucose to para-hydroxystyrene 失效
标题翻译：葡萄糖向对羟基苯乙烯的微生物转化
公开(公告)号：US07229806B2
公开(公告)日：2007-06-12
申请号：US10439478
申请日：2003-05-16
申请人： Arie Ben-Bassat , Wei Wei Qi , Fateme Sima Sariaslani , Xiao-Song Tang , Todd M. Vannelli
发明人： Arie Ben-Bassat , Wei Wei Qi , Fateme Sima Sariaslani , Xiao-Song Tang , Todd M. Vannelli
IPC分类号： C12P7/22 , C12N9/88 , C12N1/20 , C12N1/00 , C12N1/12 , C12N5/10 , C12N5/02 , C12N15/00
CPC分类号： C12P7/22 , C12N9/88
摘要： An in vivo method for the production of pHS via a recombinant host cell is disclosed. The host cell expresses at least one gene encoding a polypeptide having para-hydroxycinnamic acid decarboxylase activity in combination with either at least one gene encoding a polypeptide having tyrosine ammonia lyase activity or at least one gene encoding a polypeptide having phenylalanine ammonia lyase activity.
摘要翻译：公开了通过重组宿主细胞产生pHS的体内方法。宿主细胞表达编码具有对羟基肉桂酸脱羧酶活性的多肽的至少一种基因与至少一种编码具有酪氨酸氨裂解酶活性的多肽的基因或至少一种编码具有苯丙氨酸氨裂解酶活性的多肽的基因的组合。

56. 发明授权

US06251646B1 Method for stabilizing nitrilase activity and preserving microbial cells 有权
标题翻译：稳定腈水解酶活性和保存微生物细胞的方法
公开(公告)号：US06251646B1
公开(公告)日：2001-06-26
申请号：US09352015
申请日：1999-07-12
申请人： Robert Dicosimo , Arie Ben-Bassat , Robert D. Fallon
发明人： Robert Dicosimo , Arie Ben-Bassat , Robert D. Fallon
IPC分类号： C12N900
CPC分类号： C12P13/00 , C12N1/04 , C12N9/78 , C12N9/96 , C12N11/04 , C12N11/08 , C12N11/10
摘要： A method for the stabilization of nitrilase activity of unimmobilized or immobilized microbial cells has been developed. The unimmobilized or immobilized microbial cells are stored in an aqueous solution containing from 0.100 M to the saturation concentration of an inorganic salt of bicarbonate or carbonate, including ammonium, sodium and potassium salts of bicarbonate or carbonate. Aqueous suspensions containing at least 100 mM bicarbonate or carbonate limit microbial contamination of the stored enzyme catalyst, as well as stabilize the desired nitrilase activity of the unimmobilized or immobilized cells. Microorganisms which are characterized by an nitrilase activity and are stabilized and preserved by this method include Acidovorax facilis 72-PF-15 (ATCC 55747), Acidovorax facilis 72-PF-17 (ATCC 55745), and Acidovorax facilis 72W (ATCC 55746).
摘要翻译：已经开发了用于稳定未固定或固定的微生物细胞的腈水解酶活性的方法。将未固定或固定的微生物细胞储存在含有0.100M的水溶液中，使饱和浓度为碳酸氢盐或碳酸盐的无机盐，包括碳酸氢盐或碳酸盐的铵，钠和钾盐。含有至少100mM碳酸氢盐或碳酸氢盐的水性悬浮液限制所储存的酶催化剂的微生物污染物，以及稳定未固化或固定的细胞的所需腈水解酶活性。特征在于腈水解酶活性并通过该方法稳定和保存的微生物包括Acidovorax facilis 72-PF-15（ATCC 55747），Acidovorax facilis 72-PF-17（ATCC 55745）和Acidovorax facilis 72W（ATCC 55746）。

57. 发明授权

US5268274A Methods and nucleic acid sequences for the expression of the cellulose synthase operon 失效
标题翻译：用于表达纤维素合成酶操纵子的方法和核酸序列
公开(公告)号：US5268274A
公开(公告)日：1993-12-07
申请号：US689008
申请日：1991-04-22
申请人： Arie Ben-Bassat , Roger D. Calhoon , Anna L. Fear , David H. Gelfand , James H. Meade , Rony Tal , Hing Wong , Moshe Benziman
发明人： Arie Ben-Bassat , Roger D. Calhoon , Anna L. Fear , David H. Gelfand , James H. Meade , Rony Tal , Hing Wong , Moshe Benziman
IPC分类号： C07K14/195 , C12N1/21 , C12N9/10 , C12N9/88 , C12N15/52 , C12N15/54 , C12N15/72 , C12N15/74 , C12N15/00 , C12P19/04
CPC分类号： C12N9/1059 , C07K14/195 , C12N15/52 , C12N15/72 , C12N15/74 , C12N9/88 , C12R1/02 , Y10S435/823
摘要： Nucleic acid sequences encoding the bacterial cellulose synthase operon derived from Acetobacter are disclosed. Methods for isolating the genes, vectors containing the genes, and transformed hosts useful for the expression of recombinant bacterial cellulose synthase or production of cellulose are also described.
摘要翻译：公开了编码源自醋杆菌的细菌纤维素合成酶操纵子的核酸序列。还描述了分离基因的方法，含有该基因的载体和用于表达重组细菌纤维素合成酶或纤维素生产的转化宿主。

58. 发明授权

US5079162A Reticulated cellulose and methods and microorganisms for the production thereof 失效
标题翻译：网状纤维素及其生产方法和微生物
公开(公告)号：US5079162A
公开(公告)日：1992-01-07
申请号：US196496
申请日：1988-05-19
申请人： Arie Ben-Bassat , Robert Bruner , Sharon Shoemaker , Yehoshua Aloni , Harry Wong , Donald C. Johnson , Amar N. Neogi
发明人： Arie Ben-Bassat , Robert Bruner , Sharon Shoemaker , Yehoshua Aloni , Harry Wong , Donald C. Johnson , Amar N. Neogi
IPC分类号： A61L15/28 , C12P19/04
CPC分类号： C12R1/02 , A61L15/28 , C12P19/04 , Y10S435/823
摘要： A method and media for producing bacterial cellulose under agitated culture conditions resulting in sustained production over an average of 70 hours of at least 0.1 g/liter per hour are achieved. A unique reticulated cellulose product is produced using the methods and conditions claimed, and may be in the form of a sheet characterized by substantial resistance to densification and great tensile strength when produced by sheet forming means.Strains of Acetobacter that are stable under agitated culture conditions and that exhibit substantially reduced gluconic and keto-gluconic acids production are described.
摘要翻译：在搅拌培养条件下生产细菌纤维素的方法和介质，得到平均70小时至少0.1g /升/小时的持续生产。使用所要求保护的方法和条件制备独特的网状纤维素产品，并且可以是以片材成形方法制造时特征为显着的致密化抗拉强度的片材的形式。描述了在搅拌培养条件下稳定并且显示出显着降低的葡萄糖酸和酮 - 葡萄糖酸生产的醋杆菌菌株。

59. 发明授权

US4894334A Method of improving the yield of heterologous protein produced by cultivating recombinant bacteria 失效
标题翻译：提高培养重组细菌产生的异源蛋白质产量的方法
公开(公告)号：US4894334A
公开(公告)日：1990-01-16
申请号：US35069
申请日：1987-04-06
申请人： Arie Ben-Bassat , Glenn Dorin , Keith Bauer
发明人： Arie Ben-Bassat , Glenn Dorin , Keith Bauer
IPC分类号： C07K14/52 , C12N1/21 , C12N1/38
CPC分类号： C12N1/38 , C07K14/52
摘要： A method for improving the yield of heterologous protein such as ricin A toxin, produced by recombinant bacteria by supplementing the nutrient medium in which the bacteria are grown with an ethanol and/or amino acid mixture during the terminal phase of the cultivation. Also disclosed is a method for improving the yield of heterologous proteins under the control of the PL promoter.
摘要翻译：一种通过在培养的最后阶段补充细菌与乙醇和/或氨基酸混合物生长的营养培养基来提高重组细菌产生的异种蛋白质如蓖麻毒素A毒素的产量的方法。还公开了一种在PL启动子控制下提高异源蛋白质产量的方法。

60. 发明授权

US4865974A Bacterial methionine N-terminal peptidase 失效
标题翻译：细菌甲硫氨酸N端肽酶
公开(公告)号：US4865974A
公开(公告)日：1989-09-12
申请号：US860330
申请日：1986-05-06
申请人： Arie Ben-Bassat , Keith A. Bauer , Shing Chang , Sheng-Yung Chang
发明人： Arie Ben-Bassat , Keith A. Bauer , Shing Chang , Sheng-Yung Chang
IPC分类号： C07K14/415 , C07K14/55 , C12N1/21 , C12N9/48 , C12N15/09 , C12N15/57 , C12N15/70 , C12P21/02 , C12R1/19
CPC分类号： C12N15/70 , C07K14/415 , C07K14/55 , C12N9/48 , C12P21/02 , Y10S930/141 , Y10S930/30
摘要： Methods for obtaining N-terminal methionine-free proteins involve a novel E. coli methionine amino peptidase. The method is capable of in vitro or in vivo application. For in vivo application, a plasmid-borne DNA encoding the peptide is expressed in a bacterial host.
摘要翻译：获得N-末端不含甲硫氨酸的蛋白质的方法涉及新型大肠杆菌甲硫氨酸氨基肽酶。该方法能够在体外或体内应用。对于体内应用，编码肽的质粒携带DNA在细菌宿主中表达。

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