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41. 发明授权

US4771008A Method for quantitative analysis of thyroid hormone 失效
标题翻译：甲状腺激素定量分析方法
公开(公告)号：US4771008A
公开(公告)日：1988-09-13
申请号：US36143
申请日：1987-04-06
申请人： Kenji Miura , Yukio Yasuda
发明人： Kenji Miura , Yukio Yasuda
IPC分类号： G01N33/53 , G01N33/531 , G01N33/68 , G01N33/74 , G01N33/78
CPC分类号： G01N33/74 , G01N33/53 , G01N33/78 , Y10S436/825
摘要： A method for quantitative analysis of low molecular weight components which are contained in a protein-containing liquid sample originating from body fluids and are capable of binding to said protein, characterized by incorporating into the liquid sample a compound having the formula (I):X--(Y).sub.p --SO.sub.3 M (I)in which M is hydrogen ion, an alkali metal ion, or ammonium ion; X is a straight or branched alkyl, alkenyl, fluorinated alkyl, or fluorinated alkenyl group containing at least 6 carbon atoms, Y is a divilent organic residue; and p is 0 r 1. The compound represented by formula (I) liberates thyroid hormone from bound protein to facilitate analysis.
摘要翻译：一种用于定量分析含有源自体液的含蛋白质的液体样品中能够结合所述蛋白质的低分子量组分的方法，其特征在于向液体样品中加入具有式（I）的化合物：X - （Y）p-SO 3 M（I）其中M是氢离子，碱金属离子或铵离子; X是含有至少6个碳原子的直链或支链烷基，烯基，氟化烷基或氟化烯基，Y是疏水有机残基; 并且p为0r 1.由式（I）表示的化合物从结合的蛋白质释放甲状腺激素以促进分析。

42. 发明授权

US4760142A Divalent hapten derivatives 失效
标题翻译：二价半抗原衍生物
公开(公告)号：US4760142A
公开(公告)日：1988-07-26
申请号：US69747
申请日：1987-07-06
申请人： Kathleen J. Primes , Gerald F. Sigler , Gerd Grenner , Wolfgang Kapmeyer
发明人： Kathleen J. Primes , Gerald F. Sigler , Gerd Grenner , Wolfgang Kapmeyer
IPC分类号： G01N33/531 , G01N33/543 , G01N33/78 , G01N33/94 , C07D239/72
CPC分类号： G01N33/531 , G01N33/536 , G01N33/543 , G01N33/54313 , G01N33/78 , G01N33/94 , Y10S436/815 , Y10S436/816 , Y10S436/822 , Y10S436/823
摘要： Divalent hapten derivatives wherein two hapten moieties are connected by means of a bifunctional spacer wherein the derivative has the formulaA--X--Awhere A is a bonded hapten moiety and X is a bifunctional spacer having the formula(B).sub.m --Y--(CH.sub.2).sub.n --Z--(CH.sub.2).sub.n --Y--(B).sub.mwhere m is independently 0 or 1, B is (CH.sub.2).sub.n', wherein n' is an integer from 1 to 4, or CO(CH.sub.2).sub.n", wherein n" is an integer from 2 to 4; Y is independently --CONH--, NHCO--, OOC--, --COO--, --O--, --S--, or --NR--, wherein R is hydrogen or alkyl; n is an integer from 1 to 10; and Z is an organic moiety containing at least one hydrophilic atom are disclosed.
摘要翻译：二价半抗原衍生物，其中两个半抗原部分通过双官能间隔基连接，其中衍生物具有式AXA，其中A是键合半抗原部分，X是具有式（B）的双官能间隔基mY-（CH2）nZ-（CH2 ）nY-（B）m其中m独立地为0或1，B为（CH 2）n'，其中n'为1至4的整数，或CO（CH 2）n“，其中n”为整数从2到4; Y独立地是-CONH-，NHCO-，OOC-，-COO-，-O - ， - S - 或-NR-，其中R是氢或烷基; n是1至10的整数; 并且Z是含有至少一个亲水性原子的有机部分。

43. 发明授权

US4745072A Immunoassay and immunometric assay of free ligand concentrations in biological fluids 失效
标题翻译：生物液体中游离配体浓度的免疫测定和免疫测定
公开(公告)号：US4745072A
公开(公告)日：1988-05-17
申请号：US705421
申请日：1985-02-20
申请人： Roger P. Ekins , Thomas M. Jackson
发明人： Roger P. Ekins , Thomas M. Jackson
IPC分类号： G01N33/536 , A61K39/00 , G01N33/53 , G01N33/543 , G01N33/78 , G01N33/534 , G01N33/546
CPC分类号： G01N33/78 , G01N33/53 , Y10S436/804 , Y10S436/817
摘要： A method of measuring the concentration of a free ligand in a biological fluid containing the free ligand and ligand bound to endogenous binding agent, by the steps of(a) mixing a sample of the fluid with an analogue of the ligand, a specific binder with which the free ligand and the ligand analogue bind, and an exogenous binding agent which binds the ligand analogue but not the ligand, either the ligand analogue or the specific binder being labelled,(b) incubating the resulting mixture,(c) determining either the amount of the labelled analogue bound or the amount of labelled specific binder bound, or not bound, to the ligand analogue, and(d) correlating the determined amount to the amount of free ligand present in the sample.The method is useful to measure concentration of free thyroid hormones and other hormones in body fluids, employing antibodies specific to the ligand analogue as the exogenous binding agents.
摘要翻译： PCT No.PCT / GB84 / 00220 Sec。 371日期1985年2月20日 102（e）日期1985年2月20日PCT Filed 1984年6月22日PCT公布。公开号WO85 / 00226 1985年1月17日。一种测量含有游离配体和与内源性结合剂结合的配体的生物液体中游离配体浓度的方法，通过以下步骤：（a）将流体样品与配体，游离配体和配体类似物结合的特异性结合剂和结合配体类似物而不是配体的外源结合剂，配体类似物或被标记的特异性结合物，（b）将所得混合物，（c）确定与配体类似物结合或未结合的标记的类似物结合的量或标记的特异性结合物的量，和（d）将确定的量与样品中存在的游离配体的量相关联。该方法可用于测量体液中游离甲状腺激素和其他激素的浓度，使用对配体类似物特异的抗体作为外源性结合剂。

44. 发明授权

US4618485A Kinetic radioimmunoassay test method and device 失效
标题翻译：动力放射免疫测定方法及装置
公开(公告)号：US4618485A
公开(公告)日：1986-10-21
申请号：US357399
申请日：1982-03-12
申请人： Yuh-Geng Tsay , Vipin D. Shah
发明人： Yuh-Geng Tsay , Vipin D. Shah
IPC分类号： G01N33/543 , G01N33/557 , G01N33/56 , G01N33/78
CPC分类号： G01N33/54306 , G01N33/557 , Y10S436/804
摘要： An improved radioimmunoassay test method and device based upon competitive binding test methods wherein immunoreactions are halted at a time when the rate of change of the quantity of bound radiolabeled analyte of interest is inversely proportional to the concentration of analyte of interest in an unknown sera. Based thereon, a test device is created having a single calibration curve 36 which is accurate throughout the shelf life of the device.
摘要翻译：一种改进的放射免疫测定方法和装置，其基于竞争性结合试验方法，其中当目标结合的放射性标记的分析物的量的变化速率与未知血清中感兴趣的分析物的浓度成反比时，其中免疫反应停止。基于此，产生具有单个校准曲线36的测试装置，其在装置的整个保质期内是准确的。

45. 发明授权

US4608341A Living, fast-growing thyroid cell strain, FRTL-5 失效
标题翻译：生活，快速生长的甲状腺细胞株，FRTL-5
公开(公告)号：US4608341A
公开(公告)日：1986-08-26
申请号：US499250
申请日：1983-05-31
申请人： Francesco S. Ambesi-Impiombato
发明人： Francesco S. Ambesi-Impiombato
IPC分类号： C12N5/071 , G01N33/564 , G01N33/78 , C12N5/00 , C12R1/91
CPC分类号： G01N33/78 , C12N5/0617 , G01N33/564 , C12N2500/25 , C12N2501/18 , C12N2503/00 , Y10S435/948
摘要： A fast growing, continuous, functional rat thyroid cell strain, FRTL-5, which maintains functional characteristics of iodide uptake and thyroglobulin synthesis over prolonged periods of culture is cloned from FRTL cells obtained from primary cultures of Fischer rat thyroid glands. The FRTL-5 cells are cultured in a medium containing approximately 5 percent calf serum supplemented with a mixture of hormones, at least one of which is thyrotropin.The FRTL-5 cells are employed in a series of assays which measure thyroid stimulatory or inhibitory factors. The FRTL-5 system of assay specifically measures thymidine incorporation, cAMP elevation and iodide uptake and permits the evaluation of patient sera, particularly those afflicted with Graves' disease and other autoimmune thyroid diseases, thereby providing a means for determining appropriate methods of treatment.
摘要翻译：从Fischer大鼠甲状腺原始培养物获得的FRTL细胞克隆了快速生长，连续功能的大鼠甲状腺细胞株FRTL-5，其保持长时间培养的碘摄取和甲状腺球蛋白合成的功能特征。将FRTL-5细胞在含有补充有激素混合物的约5％小牛血清的培养基中培养，其中至少一种是促甲状腺激素。 FRTL-5细胞用于测量甲状腺刺激或抑制因子的一系列测定中。 FRTL-5分析系统特异性地测量胸苷掺入，cAMP升高和碘摄取，并允许对患者血清进行评估，特别是患有Graves病和其他自身免疫性甲状腺疾病的血清，从而提供了确定适当治疗方法的手段。

46. 发明授权

US4521522A Optical specific binding assay with reflection of polarized electromagnetic radiation 失效
标题翻译：具有极化电磁辐射反射的光学特异性结合测定
公开(公告)号：US4521522A
公开(公告)日：1985-06-04
申请号：US415510
申请日：1982-09-07
申请人： Kurt I. Lundstrom , Hans R. Arwin , Erwin Rieke , Gunter Sielaff , Norbert Hennrich
发明人： Kurt I. Lundstrom , Hans R. Arwin , Erwin Rieke , Gunter Sielaff , Norbert Hennrich
IPC分类号： G01N21/21 , G01N33/543 , G01N33/54 , G01N33/78
CPC分类号： G01N33/54373 , G01N21/21 , Y10S436/805
摘要： A process, means and devices for the quantitative determination, using electromagnetic radiation, of a component of a group including specifically bonding receptors and substances which can be specifically bonded by these receptors are based on a process comprising incubating one of the components, after it is immobilizing on the surface of a material having refractive index different from that of the components, with the other particular component and, optionally, further components, which can be labelled; irradiating the surface with electromagnetic radiation which is polarized parallel to the plane of incidence, the angle of incidence of the radiation being approximately equal to the angle .PHI. at which the intensity of the radiation reflected from the uncoated or precoated surface reaches a minimum; and ascertaining the intensity of the reflected radiation as a measure of the concentration to be determined.
摘要翻译：使用电磁辐射定量测定包括特异性结合受体和能够被这些受体特异性结合的物质的组分的方法，装置和装置是基于包括孵育其中一种组分的方法固定在具有不同于组分的折射率的材料的表面上，与其他特定组分和任选地可以标记的其它组分; 用平行于入射平面极化的电磁辐射照射表面，辐射入射角近似等于从未涂覆或预涂表面反射的辐射强度达到最小值的角度PHI; 并确定反射辐射的强度作为待测浓度的量度。

47. 发明授权

US4506019A Activated polymer container means and assay method employing the same 失效
标题翻译：活性聚合物容器的方法和采用该方法的测定方法
公开(公告)号：US4506019A
公开(公告)日：1985-03-19
申请号：US422801
申请日：1982-09-24
申请人： Jin P. Lee
发明人： Jin P. Lee
IPC分类号： G01N33/545 , G01N33/78 , G01N33/54 , B65D71/00 , G01N23/06
CPC分类号： G01N33/78 , G01N33/545 , Y10S436/81 , Y10S436/823
摘要： Novel activated polyamide polymer container or test tube means and a method for the determination of ligand or analyte content in aqueous samples, are provided. The surfaces of the container tubes are specially activated thermally during their manufacture by injection molding thereby avoiding the need for subsequent coating of the tubes, e.g., with antibody, for binding assay procedures.
摘要翻译：提供了新型活性聚酰胺聚合物容器或试管的方法和用于测定含水样品中配体或分析物含量的方法。容器管的表面在其通过注射成型制造期间被特别地热活化，从而避免了随后涂覆管的需要，例如用抗体，用于结合测定程序。

48. 发明授权

US4480041A Use of phosphotriester intermediates for preparation of functionalized liposomes 失效
标题翻译：使用磷酸三酯中间体制备官能化脂质体
公开(公告)号：US4480041A
公开(公告)日：1984-10-30
申请号：US396678
申请日：1982-07-09
申请人： Arthur Myles , Say-Jong Law , Frank X. Cole
发明人： Arthur Myles , Say-Jong Law , Frank X. Cole
IPC分类号： G01N33/58 , G01N33/54 , G01N33/78
CPC分类号： G01N33/586 , Y10S436/816 , Y10S436/817 , Y10S436/829
摘要： A method of forming an analyte-functionalized liposome for use in immunoassay techniques employs a phosphotriester intermediate of a phospholipid derivatized with the desired analyte in a process of forming a liposome which is functionalized on its outer surface with the analyte and which carries an enzyme marker. The method is also used to functionalize the liposome with a ligand which acts as a leash between the analyte and the liposome. Liposomes produced by the method include penicillin-G-functionalized liposome.
摘要翻译：形成用于免疫测定技术的分析物官能化的脂质体的方法使用在形成脂质体的过程中用所需分析物衍生的磷脂的磷酸三酯中间体，其在其外表面上与分析物官能化并携带酶标记。该方法还用于用作为分析物和脂质体之间的皮带的配体官能化脂质体。通过该方法生产的脂质体包括青霉素-G官能化的脂质体。

49. 发明授权

US4391795A Assay for free thyroid hormone 失效
标题翻译：测定游离甲状腺激素
公开(公告)号：US4391795A
公开(公告)日：1983-07-05
申请号：US131934
申请日：1980-03-21
申请人： Samuel R. Pearlman
发明人： Samuel R. Pearlman
IPC分类号： G01N33/78 , G01N33/56 , G01N33/58 , G01N33/60
CPC分类号： G01N33/78 , Y10S436/808
摘要： Sample containing free and bound thyroid hormone; in particular, thyroxine, is contacted with immobilized binder; in particular, supported on a solid support, to bind the free thyroxine. After separating the bound thyroxine from the immobilized binder having free thyroxine bound thereto, the immobilized binder is contacted with labeled thyroxine; in particular, radiolabeled thyroxine. Unbound labeled thyroxine is removed, and the amount of bound and/or unbound labeled thyroxine is measured, with the free thyroxine concentration in the sample being inversely proportional to the amount of labeled thyroxine bound to the immobilized binder.
摘要翻译：含有游离和结合甲状腺激素的样品; 特别是甲状腺素与固定化的粘合剂接触; 特别是支持坚定的支持，约束游离甲状腺素。将结合的甲状腺素与结合有游离甲状腺素的固定化粘合剂分离后，将固定的粘合剂与标记的甲状腺素接触; 特别是放射性标记的甲状腺素。去除未结合的标记的甲状腺素，测量结合和/或未结合标记的甲状腺素的量，样品中的游离甲状腺素浓度与与固定化的结合物结合的标记的甲状腺素的量成反比。

50. 发明授权

US4347058A Thyroid polarization fluoroimmunoassay 失效
标题翻译：甲状腺偏光荧光免疫测定
公开(公告)号：US4347058A
公开(公告)日：1982-08-31
申请号：US187238
申请日：1980-09-15
申请人： Alan J. Polito , Kurtis R. Bray
发明人： Alan J. Polito , Kurtis R. Bray
IPC分类号： G01N33/78 , G01N33/52 , G01N33/54
CPC分类号： G01N33/78 , Y10S436/80
摘要： A polarization fluoroimmunoassay for T.sub.3 or T.sub.4 thyroid hormones where a serum sample is assayed by contacting the sample with a base to denature the thyroxine binding globulins and thereby form a solution, incubating the solution, contacting the solution with a separating agent and separating bound thyroid hormone from the serum, contacting the separating agent with bound thyroid hormone with an antibody against the thyroid hormone, incubating the separating agent-antibody combination, separating free antibody and thyroid hormone bound antibody from the separating agent, contacting the free antibody with fluorescent labelled thyroid hormone and measuring the fluorescence polarization as an indication of the thyroid hormone in the sample.
摘要翻译：用于T3或T4甲状腺激素的偏振荧光免疫测定法，其中通过使样品与碱接触来测定血清样品，以使甲状腺素结合球蛋白变性，从而形成溶液，孵育溶液，使溶液与分离剂接触并分离结合的甲状腺激素从血清中将分离剂与结合的甲状腺激素与抗甲状腺激素的抗体接触，孵育分离剂 - 抗体组合，从分离剂中分离游离抗体和甲状腺激素结合的抗体，使游离抗体与荧光标记的甲状腺激素接触并测量荧光偏振作为样品中甲状腺激素的指示。

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