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1. 发明授权

US4608341A Living, fast-growing thyroid cell strain, FRTL-5 失效
标题翻译：生活，快速生长的甲状腺细胞株，FRTL-5
公开(公告)号：US4608341A
公开(公告)日：1986-08-26
申请号：US499250
申请日：1983-05-31
申请人： Francesco S. Ambesi-Impiombato
发明人： Francesco S. Ambesi-Impiombato
IPC分类号： C12N5/071 , G01N33/564 , G01N33/78 , C12N5/00 , C12R1/91
CPC分类号： G01N33/78 , C12N5/0617 , G01N33/564 , C12N2500/25 , C12N2501/18 , C12N2503/00 , Y10S435/948
摘要： A fast growing, continuous, functional rat thyroid cell strain, FRTL-5, which maintains functional characteristics of iodide uptake and thyroglobulin synthesis over prolonged periods of culture is cloned from FRTL cells obtained from primary cultures of Fischer rat thyroid glands. The FRTL-5 cells are cultured in a medium containing approximately 5 percent calf serum supplemented with a mixture of hormones, at least one of which is thyrotropin.The FRTL-5 cells are employed in a series of assays which measure thyroid stimulatory or inhibitory factors. The FRTL-5 system of assay specifically measures thymidine incorporation, cAMP elevation and iodide uptake and permits the evaluation of patient sera, particularly those afflicted with Graves' disease and other autoimmune thyroid diseases, thereby providing a means for determining appropriate methods of treatment.
摘要翻译：从Fischer大鼠甲状腺原始培养物获得的FRTL细胞克隆了快速生长，连续功能的大鼠甲状腺细胞株FRTL-5，其保持长时间培养的碘摄取和甲状腺球蛋白合成的功能特征。将FRTL-5细胞在含有补充有激素混合物的约5％小牛血清的培养基中培养，其中至少一种是促甲状腺激素。 FRTL-5细胞用于测量甲状腺刺激或抑制因子的一系列测定中。 FRTL-5分析系统特异性地测量胸苷掺入，cAMP升高和碘摄取，并允许对患者血清进行评估，特别是患有Graves病和其他自身免疫性甲状腺疾病的血清，从而提供了确定适当治疗方法的手段。

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