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41. 发明授权

US09017945B2 Method for sequencing a polynucleotide template 有权
标题翻译：多核苷酸模板测序方法
公开(公告)号：US09017945B2
公开(公告)日：2015-04-28
申请号：US13556053
申请日：2012-07-23
申请人： Geoffrey Paul Smith
发明人： Geoffrey Paul Smith
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q2535/119
摘要： The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which permit the sequential determination of nucleotide sequences in two distinct and separate regions on complementary strands of the double-stranded polynucleotide template. The two regions for sequence determination may or may not be complementary to each other.
摘要翻译：本发明涉及用于双链多核苷酸模板的成对测序的方法，其允许在双链多核苷酸模板的互补链上的两个不同和分离的区域中顺序测定核苷酸序列。用于序列确定的两个区域可以彼此或可以不互补。

42. 发明授权

US08999642B2 Methods for selecting and amplifying polynucleotides 有权
标题翻译：选择和扩增多核苷酸的方法
公开(公告)号：US08999642B2
公开(公告)日：2015-04-07
申请号：US13387078
申请日：2009-08-25
申请人： Andrea Sabot , Roberto Rigatti , Min-Jui Richard Shen
发明人： Andrea Sabot , Roberto Rigatti , Min-Jui Richard Shen
IPC分类号： C12Q1/68 , C40B50/06 , C40B50/14 , C07H21/02 , C07H21/04
CPC分类号： C12N15/1068 , C12Q1/6806 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2565/507 , C12Q2531/107
摘要： The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.
摘要翻译：本发明提供了控制固体支持物表面上不同分子种类的密度的方法。将不同分子种类的第一混合物连接到固体支持物上，以便以期望的密度附着每种物质，从而产生具有附着捕获分子的衍生化载体。衍生的载体用不同分子种类的第二混合物处理，其中第二混合物中的不同分子物质特异性地结合到固相载体上的不同捕获分子。一个或多个捕获分子可以被可逆地修饰，使得捕获分子在分子物质的第二混合物附着之前和之后具有不同的活性。在具体实施方案中，不同的分子种类是可逆修饰以在扩增反应中具有不同活性的核酸。

43. 发明授权

US08884211B2 Confocal imaging methods and apparatus 有权
标题翻译：共焦成像方法和仪器
公开(公告)号：US08884211B2
公开(公告)日：2014-11-11
申请号：US13417474
申请日：2012-03-12
申请人： Wenyi Feng , Theofilos Kotseroglou , Mark Wang , Alexander Triener , Diping Che , Robert Kain
发明人： Wenyi Feng , Theofilos Kotseroglou , Mark Wang , Alexander Triener , Diping Che , Robert Kain
IPC分类号： H01L27/00 , G01N21/64 , G02B21/00
CPC分类号： G01N21/6456 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6421 , G02B21/0032 , G02B21/004
摘要： The invention provides imaging apparatus and methods useful for obtaining a high resolution image of a sample at rapid scan rates. A rectangular detector array having a horizontal dimension that is longer than the vertical dimension can be used along with imaging optics positioned to direct a rectangular image of a portion of a sample to the rectangular detector array. A scanning device can be configured to scan the sample in a scan-axis dimension, wherein the vertical dimension for the rectangular detector array and the shorter of the two rectangular dimensions for the image are in the scan-axis dimension, and wherein the vertical dimension for the rectangular detector array is short enough to achieve confocality in a single axis.
摘要翻译：本发明提供了用于以快速扫描速率获得样品的高分辨率图像的成像装置和方法。具有比垂直尺寸长的水平尺寸的矩形检测器阵列可以与定位成将样本的一部分的矩形图像引导到矩形检测器阵列的成像光学元件一起使用。扫描装置可以被配置成以扫描轴尺寸扫描样本，其中矩形检测器阵列的垂直尺寸和图像的两个矩形尺寸中的较短的尺寸在扫描轴尺寸上，并且其中垂直尺寸因为矩形检测器阵列足够短以在单个轴上实现共焦。

44. 发明授权

US08822150B2 Methods for indexing samples and sequencing multiple polynucleotide templates 有权
标题翻译：索引样本和测序多个多核苷酸模板的方法
公开(公告)号：US08822150B2
公开(公告)日：2014-09-02
申请号：US13444136
申请日：2012-04-11
申请人： Helen Bignell , Louise Fraser , Niall Anthony Gormley
发明人： Helen Bignell , Louise Fraser , Niall Anthony Gormley
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6874 , C12Q1/6837 , C12Q1/6855 , C12Q2537/143 , C12Q2525/161
摘要： The invention relates to methods for indexing samples during the sequencing of polynucleotide templates, resulting in the attachment of tags specific to the source of each nucleic acid sample such that after a sequencing run, both the source and sequence of each polynucleotide can be determined. Thus, the present invention pertains to analysis of complex genomes (e.g., human genomes), as well as multiplexing less complex genomes, such as those of bacteria, viruses, mitochondria, and the like.
摘要翻译：本发明涉及在多核苷酸模板的测序期间对样品进行索引的方法，导致特异于每个核酸样品的来源的标签的附着，使得在测序运行之后，可以确定每个多核苷酸的来源和序列。因此，本发明涉及复杂基因组（例如，人类基因组）的分析，以及复合较不复杂的基因组，例如细菌，病毒，线粒体等的分析。

45. 发明授权

US08765381B2 Method for pairwise sequencing of target polynucleotides 有权
标题翻译：靶多核苷酸成对测序的方法
公开(公告)号：US08765381B2
公开(公告)日：2014-07-01
申请号：US13857027
申请日：2013-04-04
申请人： Illumina Cambridge Limited
发明人： Roberto Rigatti , Tobias William Barr Ost
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6874 , C12Q2535/119 , C12Q2535/122 , C12Q2565/513 , C12Q2565/543
摘要： The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.
摘要翻译：本发明涉及用于双链多核苷酸模板的成对测序的方法，该方法导致多核苷酸模板的两个不同和分离的区域中核苷酸序列的顺序测定。使用本发明的方法，可以从聚簇阵列上的每个双链模板获得序列信息的两个链接或配对读取，而不仅仅是从模板的一条链读取的单个测序。

46. 发明授权

US08728764B2 Nucleic acid sample enrichment for sequencing applications 有权
标题翻译：用于测序应用的核酸样品富集
公开(公告)号：US08728764B2
公开(公告)日：2014-05-20
申请号：US13119131
申请日：2009-10-02
申请人： Jonathan Mark Boutell
发明人： Jonathan Mark Boutell
IPC分类号： C12P19/34
CPC分类号： C12Q1/6874 , C12Q1/6806 , C12Q1/686 , C12Q2531/113 , C12Q2565/501 , C12Q2525/161
摘要： The present invention relates to the field of molecular biology, and more specifically to methods for reducing the complexity of a nucleic acid sample.
摘要翻译：本发明涉及分子生物学领域，更具体地涉及降低核酸样品复杂性的方法。

47. 发明授权

US08652810B2 Methods of nucleic acid amplification and sequencing 有权
标题翻译：核酸扩增和测序方法
公开(公告)号：US08652810B2
公开(公告)日：2014-02-18
申请号：US11506146
申请日：2006-08-17
申请人： Celine Adessi , Eric Kawashima , Pascal Mayer , Jean-Jacques Mermod , Gerardo Turcatti
发明人： Celine Adessi , Eric Kawashima , Pascal Mayer , Jean-Jacques Mermod , Gerardo Turcatti
IPC分类号： C12P19/34 , C12Q1/68 , C12M1/34 , C12M3/00 , C07H21/02 , C07H21/04 , C07H21/00
CPC分类号： C12N15/1068 , C12Q1/6837 , C12Q1/686 , C12Q1/6869 , C12Q1/6874 , C12Q2565/543
摘要： Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) contains at the 5′ end an oligonucleotide sequence Y and at the 3′ end an oligonucleotide sequence Z and, in addition, the nucleic acid(s) carry at the 5′ end a means for attaching the nucleic acid(s) to a solid support; (2) mixing said nucleic acid template(s) with one or more colony primers X, which can hybridize to the oligonucleotide sequence Z and carries at the 5′ end a means for attaching the colony primers to a solid support, in the presence of a solid support so that the 5′ ends of both the nucleic acid template and the colony primers bind to the solid support; (3) performing one or more nucleic acid amplification reactions on the bound template(s), so that nucleic acid colonies are generated and optionally, performing at least one step of sequence determination of one or more of the nucleic acid colonies generated. Solid supports, kits and apparatus for use in these methods.
摘要翻译：用于扩增和测序至少一种核酸的方法，包括以下步骤：（1）形成至少一个核酸模板，其包含待扩增或测序的核酸，其中所述核酸在5 '结束寡核苷酸序列Y，在3'端有寡核苷酸序列Z，另外核酸在5'端带有用于将核酸连接到固体支持物上的方法; （2）将所述核酸模板与一个或多个菌落引物X混合，其可与寡核苷酸序列Z杂交，并在5'末端携带用于将菌落引物连接至固体支持物的手段，在存在固体支持物，使得核酸模板和集落引物的5'末端与固体支持物结合; （3）对结合的模板进行一个或多个核酸扩增反应，从而产生核酸集落，并且任选地，进行生成的一个或多个核酸集落的至少一个序列测定步骤。用于这些方法的固体支撑物，试剂盒和仪器。

48. 发明授权

US11697847B2 Super resolution imaging 有权
公开(公告)号：US11697847B2
公开(公告)日：2023-07-11
申请号：US16837463
申请日：2020-04-01
申请人： Illumina, Inc.
发明人： Robert C. Kain , Tarun Khurana , Kevin L. Gunderson , Yir-Shyuan Wu
IPC分类号： G01N21/64 , C12Q1/6825 , C12Q1/6874 , C12Q1/6837
CPC分类号： C12Q1/6874 , C12Q1/6825 , C12Q1/6837 , G01N21/6454 , C12Q1/6825 , C12Q2563/103 , C12Q2563/113 , C12Q2563/179 , C12Q2565/101 , C12Q2565/101 , C12Q2565/607 , C12Q1/6837 , C12Q2563/103 , C12Q2563/113 , C12Q2563/179 , C12Q2565/101 , C12Q2565/101 , C12Q2565/607 , C12Q1/6874 , C12Q2563/103 , C12Q2563/113 , C12Q2563/179 , C12Q2565/101 , C12Q2565/101 , C12Q2565/607
摘要： A detection apparatus that includes (a) an array of responsive pads on a substrate surface; (b) an array of pixels, wherein each pixel in the array has a detection zone on the surface that includes a subset of at least two of the pads; and (c) an activation circuit to apply a force at a first and second pad in the subset, wherein the activation circuit is configured to apply a different force at the first pad compared to the second pad, and wherein the activation circuit has a switch to selectively alter the force at the first pad and the second pad.

49. 发明授权

US11585757B2 Device for luminescent imaging 有权
公开(公告)号：US11585757B2
公开(公告)日：2023-02-21
申请号：US16439635
申请日：2019-06-12
申请人： Illumina, Inc.
发明人： Dajun Yuan , Liangliang Qiang , Minghao Guo
IPC分类号： G01N21/64 , G02F1/1335 , G02B26/00 , C12Q1/6869
摘要： A device includes a plurality of imaging pixels in a spatial pattern with a formation of features disposed over the pixels. A first and a second feature of the formation of features are disposed over a first pixel. A first luminophore is disposed within or over the first feature. A second luminophore is disposed within or over the second feature. A structured illumination source is to direct at least a portion of first photons in an illumination pattern to the first feature at a first time, and to direct at least a portion of second photons in the illumination pattern to the second feature at a second time. The structured illumination source includes an illumination pattern generator having an illumination pattern generator actuator connected to the illumination pattern generator to cause the illumination pattern to translate or rotate relative to the formation of features.

50. 发明授权

US11512339B2 Arrays including a resin film and a patterned polymer layer 有权
公开(公告)号：US11512339B2
公开(公告)日：2022-11-29
申请号：US15848640
申请日：2017-12-20
申请人： ILLUMINA, INC. , ILLUMINA CAMBRIDGE LIMITED
发明人： Wayne N. George , Alexandre Richez , M. Shane Bowen , Andrew A. Brown , Dajun Yuan , Audrey Rose Zak , Sean M. Ramirez , Raymond Campos
IPC分类号： C09D183/06 , C12Q1/68 , C09D133/26 , C08G77/14
摘要： An example of an array includes a support, a cross-linked epoxy polyhedral oligomeric silsesquioxane (POSS) resin film on a surface of the support, and a patterned hydrophobic polymer layer on the cross-linked epoxy POSS resin film. The patterned hydrophobic polymer layer defines exposed discrete areas of the cross-linked epoxy POSS resin film, and a polymer coating is attached to the exposed discrete areas. Another example of an array includes a support, a modified epoxy POSS resin film on a surface of the support, and a patterned hydrophobic polymer layer on the modified epoxy POSS resin film. The modified epoxy POSS resin film includes a polymer growth initiation site, and the patterned hydrophobic polymer layer defines exposed discrete areas of the modified epoxy POSS resin film. A polymer brush is attached to the polymer growth initiation site in the exposed discrete areas.

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