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1. 发明授权

US08999642B2 Methods for selecting and amplifying polynucleotides 有权
标题翻译：选择和扩增多核苷酸的方法
公开(公告)号：US08999642B2
公开(公告)日：2015-04-07
申请号：US13387078
申请日：2009-08-25
申请人： Andrea Sabot , Roberto Rigatti , Min-Jui Richard Shen
发明人： Andrea Sabot , Roberto Rigatti , Min-Jui Richard Shen
IPC分类号： C12Q1/68 , C40B50/06 , C40B50/14 , C07H21/02 , C07H21/04
CPC分类号： C12N15/1068 , C12Q1/6806 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2565/507 , C12Q2531/107
摘要： The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.
摘要翻译：本发明提供了控制固体支持物表面上不同分子种类的密度的方法。将不同分子种类的第一混合物连接到固体支持物上，以便以期望的密度附着每种物质，从而产生具有附着捕获分子的衍生化载体。衍生的载体用不同分子种类的第二混合物处理，其中第二混合物中的不同分子物质特异性地结合到固相载体上的不同捕获分子。一个或多个捕获分子可以被可逆地修饰，使得捕获分子在分子物质的第二混合物附着之前和之后具有不同的活性。在具体实施方案中，不同的分子种类是可逆修饰以在扩增反应中具有不同活性的核酸。

2. 发明申请

US20120122737A1 METHODS FOR SELECTING AND AMPLIFYING POLYNUCLEOTIDES 有权
标题翻译：选择和放大多核苷酸的方法
公开(公告)号：US20120122737A1
公开(公告)日：2012-05-17
申请号：US13387078
申请日：2009-08-25
申请人： Andrea Sabot , Roberto Rigatti , Min-Jui Richard Shen
发明人： Andrea Sabot , Roberto Rigatti , Min-Jui Richard Shen
IPC分类号： C40B50/06 , C40B50/18 , C40B60/14
CPC分类号： C12N15/1068 , C12Q1/6806 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2565/507 , C12Q2531/107
摘要： The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.
摘要翻译：本发明提供了控制固体支持物表面上不同分子种类的密度的方法。将不同分子种类的第一混合物连接到固体支持物上，以便以期望的密度附着每种物质，从而产生具有附着捕获分子的衍生化载体。衍生的载体用不同分子种类的第二混合物处理，其中第二混合物中的不同分子物质特异性地结合到固相载体上的不同捕获分子。一个或多个捕获分子可以被可逆地修饰，使得捕获分子在分子物质的第二混合物附着之前和之后具有不同的活性。在具体实施方案中，不同的分子种类是可逆修饰以在扩增反应中具有不同活性的核酸。

3. 发明申请

US20090226975A1 CONSTANT CLUSTER SEEDING 审中-公开
标题翻译：恒久集群种子
公开(公告)号：US20090226975A1
公开(公告)日：2009-09-10
申请号：US12395299
申请日：2009-02-27
申请人： Andrea Sabot , Roberto Rigatti , Min-Jui Richard Shen
发明人： Andrea Sabot , Roberto Rigatti , Min-Jui Richard Shen
IPC分类号： C12P19/34
CPC分类号： C12Q1/6837 , C12Q2565/543
摘要： The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.
摘要翻译：本发明提供了控制固体支持物表面上不同分子种类的密度的方法。将不同分子种类的第一混合物连接到固体支持物上，以便以期望的密度附着每种物质，从而产生具有附着捕获分子的衍生化载体。衍生的载体用不同分子种类的第二混合物处理，其中第二混合物中的不同分子物质特异性地结合到固相载体上的不同捕获分子。一个或多个捕获分子可以被可逆地修饰，使得捕获分子在分子物质的第二混合物附着之前和之后具有不同的活性。在具体实施方案中，不同的分子种类是可逆修饰以在扩增反应中具有不同活性的核酸。

4. 发明申请

US20130071879A1 AMPLIFICATION METHODS TO MINIMISE SEQUENCE SPECIFIC BIAS 有权
标题翻译：最小化序列特异性偏差的放大方法
公开(公告)号：US20130071879A1
公开(公告)日：2013-03-21
申请号：US13580336
申请日：2011-02-23
申请人： Roberto Rigatti , Jonathan Mark Boutell , Min-Jui Richard Shen
发明人： Roberto Rigatti , Jonathan Mark Boutell , Min-Jui Richard Shen
IPC分类号： C12P19/34 , C09K3/00
CPC分类号： C12P19/34 , C09K3/00 , C12Q1/686 , C12Q2565/518 , C12Q2527/137
摘要： Methods for amplifying nucleic acids are provided. The methods can be used to minimise sequence specific bias caused by the preferential amplification of certain nucleic acid sequences. Methods are described which can lower the efficiency of AT rich templates relative to GC rich templates, thereby minimising GC bias during amplification reactions with multiple templates of different sequence. The methods are suited to solid phase amplification, for example, utilising flow cells.
摘要翻译：提供了扩增核酸的方法。这些方法可以用于最小化由某些核酸序列的优先扩增引起的序列特异性偏差。描述了可以降低AT富含模板相对于GC富集模板的效率的方法，由此使具有不同序列的多个模板的扩增反应期间的GC偏差最小化。该方法适用于固相扩增，例如利用流动池。

5. 发明授权

US08759037B2 Amplification methods to minimise sequence specific bias 有权
标题翻译：使序列特异性偏差最小化的扩增方法
公开(公告)号：US08759037B2
公开(公告)日：2014-06-24
申请号：US13580336
申请日：2011-02-23
申请人： Roberto Rigatti , Jonathan Mark Boutell , Min-Jui Richard Shen
发明人： Roberto Rigatti , Jonathan Mark Boutell , Min-Jui Richard Shen
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C09K3/00 , C12Q1/686 , C12Q2565/518 , C12Q2527/137
摘要： Methods for amplifying nucleic acids are provided. The methods can be used to minimize sequence specific bias caused by the preferential amplification of certain nucleic acid sequences. Methods are described which can lower the efficiency of AT rich templates relative to GC rich templates, thereby minimizing GC bias during amplification reactions with multiple templates of different sequence. The methods are suited to solid phase amplification, for example, utilizing flow cells.
摘要翻译：提供了扩增核酸的方法。这些方法可以用于最小化由某些核酸序列的优先扩增引起的序列特异性偏差。描述了可以降低AT富含模板相对于GC富集模板的效率的方法，由此使具有不同序列的多个模板的扩增反应期间的GC偏差最小化。该方法适用于固相扩增，例如利用流动池。

6. 发明授权

US08399192B2 Using populations of beads for the fabrication of arrays on surfaces 有权
标题翻译：使用珠子的种群在表面上制造阵列
公开(公告)号：US08399192B2
公开(公告)日：2013-03-19
申请号：US13365562
申请日：2012-02-03
申请人： Roberto Rigatti , Geoffrey Paul Smith , Jonathan Mark Boutell
发明人： Roberto Rigatti , Geoffrey Paul Smith , Jonathan Mark Boutell
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N15/1068 , B01J19/0046 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2565/537 , C12Q2563/149 , C12Q2563/161
摘要： The present invention provides a method of creating an array of features. The method can include steps of (a) providing a plurality of beads, wherein each bead in the plurality of beads includes probe content; (b) contacting the plurality of beads with a surface to produce a layer of beads on the surface; and (c) transferring the probe content from the beads to the surface to create an array of spatially discrete features on the surface, wherein each spatially discrete feature includes probe content from a bead in the plurality of beads.
摘要翻译：本发明提供了一种创建特征阵列的方法。该方法可以包括以下步骤：（a）提供多个珠，其中多个珠中的每个珠包括探针含量; （b）使多个珠粒与表面接触以在表面上产生一层珠粒; 和（c）将探针含量从珠粒转移到表面以在表面上产生空间上离散的特征的阵列，其中每个空间上离散的特征包括来自多个珠粒中的珠粒的探针含量。

7. 发明申请

US20120172259A1 USING POPULATIONS OF BEADS FOR THE FABRICATION OF ARRAYS ON SURFACES 有权
标题翻译：使用人造丝来制作表面阵列
公开(公告)号：US20120172259A1
公开(公告)日：2012-07-05
申请号：US13365562
申请日：2012-02-03
申请人： Roberto Rigatti , Geoffrey Paul Smith , Jonathan Mark Boutell
发明人： Roberto Rigatti , Geoffrey Paul Smith , Jonathan Mark Boutell
IPC分类号： C40B50/18 , C40B50/06
CPC分类号： C12N15/1068 , B01J19/0046 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2565/537 , C12Q2563/149 , C12Q2563/161
摘要： The present invention provides a method of creating an array of features. The method can include steps of (a) providing a plurality of beads, wherein each bead in the plurality of beads includes probe content; (b) contacting the plurality of beads with a surface to produce a layer of beads on the surface; and (c) transferring the probe content from the beads to the surface to create an array of spatially discrete features on the surface, wherein each spatially discrete feature includes probe content from a bead in the plurality of beads.
摘要翻译：本发明提供了一种创建特征阵列的方法。该方法可以包括以下步骤：（a）提供多个珠，其中多个珠中的每个珠包括探针含量; （b）使多个珠粒与表面接触以在表面上产生一层珠粒; 和（c）将探针含量从珠粒转移到表面以在表面上产生空间上离散的特征的阵列，其中每个空间上离散的特征包括来自多个珠粒中的珠粒的探针含量。

8. 发明申请

US20110009276A1 Method for Sequencing a Polynucleotide Template 有权
标题翻译：多核苷酸模板测序方法
公开(公告)号：US20110009276A1
公开(公告)日：2011-01-13
申请号：US12223759
申请日：2007-02-08
申请人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias Willaim Barr Ost , David Bentley
发明人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias Willaim Barr Ost , David Bentley
IPC分类号： C40B30/00
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q2565/518 , C12Q2537/149
摘要： The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
摘要翻译：本发明提供了双链多核苷酸模板的成对测序的方法，该方法导致多核苷酸模板的两个不同和分离的区域中的核苷酸序列的顺序测定。

9. 发明授权

US07741463B2 Method of preparing libraries of template polynucleotides 有权
标题翻译：制备模板多核苷酸文库的方法
公开(公告)号：US07741463B2
公开(公告)日：2010-06-22
申请号：US11486953
申请日：2006-07-14
申请人： Niall Anthony Gormley , Geoffrey Paul Smith , David Bentley , Roberto Rigatti , Shujun Luo
发明人： Niall Anthony Gormley , Geoffrey Paul Smith , David Bentley , Roberto Rigatti , Shujun Luo
IPC分类号： C07H21/02 , G01N33/00 , C12P19/34
CPC分类号： C12Q1/6855 , C12N15/1068 , C12N15/1096 , C12Q1/686 , C40B40/06 , Y10T436/143333 , C12Q2565/525 , C12Q2525/313
摘要： The present invention relates to a method for preparing a library of template polynucleotides and use thereof in methods of solid-phase nucleic acid amplification. More specifically, the invention relates to a method for preparing a library of template polynucleotides that have common sequences at their 5′ ends and at their 3′ ends.
摘要翻译：本发明涉及一种制备模板多核苷酸文库的方法及其在固相核酸扩增方法中的应用。更具体地，本发明涉及一种制备在其5'末端和3'末端具有共同序列的模板多核苷酸文库的方法。

10. 发明申请

US20090176662A1 End Modification to Prevent Over-Representation of Fragments 有权
标题翻译：最终修改以防止片段过度表示
公开(公告)号：US20090176662A1
公开(公告)日：2009-07-09
申请号：US12223761
申请日：2007-02-07
申请人： Roberto Rigatti , Niall Anthony Gormley , Helen Rachel Bignell
发明人： Roberto Rigatti , Niall Anthony Gormley , Helen Rachel Bignell
IPC分类号： C40B40/08 , C40B50/06
CPC分类号： C12Q1/6806 , C12N15/1093 , C12Q1/6855 , C12Q2525/119 , C12Q2525/186
摘要： The invention relates to a method of preparing a 5′ and 3′ modified library of template polynucleotides and also the use of the 5′ and 3′ modified library of templates in methods of solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a 5′ and 3′ modified library of template polynucleotides which have common sequences at their 5′ ends and at their 3′ ends, wherein over-representation of “end” sequences of the primary polynucleotide molecules from whence the 5′ and 3′ modified library is generated is greatly reduced or prevented.
摘要翻译：本发明涉及一种制备模板多核苷酸的5'和3'修饰文库的方法，以及在固相核酸扩增方法中使用5'和3'修饰的模板文库。特别地，本发明涉及一种制备模板多核苷酸的5'和3'修饰文库的方法，该文库在其5'末端和3'末端具有共同序列，其中主要“末端”序列的过表达产生5'和3'修饰的文库的多核苷酸分子大大降低或防止。

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