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21. 发明授权

US09670510B2 Methods of hydrolyzing and fermenting cellulosic material 有权
公开(公告)号：US09670510B2
公开(公告)日：2017-06-06
申请号：US14240663
申请日：2012-09-07
申请人： Hui Xu , Ye Chen , Xin Li , Mark Stevens , Zhengfang Kang
发明人： Hui Xu , Ye Chen , Xin Li , Mark Stevens , Zhengfang Kang
IPC分类号： C12P7/14 , C12P19/14 , C12P7/10
CPC分类号： C12P7/14 , C12P7/10 , C12P19/14 , Y02E50/16
摘要： Described herein are improved methods of degrading or converting cellulosic material into fermentable sugars using dithionite. Also described are improved methods of fermentation in the presence of dithionite.

22. 发明授权

US09663808B2 Compositions comprising a polypeptide having cellulolytic enhancing activity and an organic compound and uses thereof 有权
公开(公告)号：US09663808B2
公开(公告)日：2017-05-30
申请号：US14500641
申请日：2014-09-29
申请人： Novozymes, Inc. , Novozymes A/S
发明人： Jason Quinlan , Feng Xu , Matthew Sweeney , Katja Salomon Johansen
IPC分类号： C12P19/14 , C12P7/10 , C12P19/02 , C12N9/42 , C12N9/24 , C12N9/96 , C12P7/14 , B01J31/06
CPC分类号： C12P19/14 , B01J31/06 , C12N9/2402 , C12N9/2437 , C12N9/2445 , C12N9/96 , C12P7/10 , C12P7/14 , C12P19/02 , C12P2203/00 , C12Y302/01021 , Y02E50/16 , Y02P20/52
摘要： The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and an organic compound. The present invention also relates to methods of using the compositions.

23. 发明申请

US20170145445A1 Process and Systems for the Fermentative Production of Alcohols 审中-公开
公开(公告)号：US20170145445A1
公开(公告)日：2017-05-25
申请号：US15293599
申请日：2016-10-14
申请人： BUTAMAX ADVANCED BIOFUELS LLC
发明人： Stephane Francois Bazzana , James Timothy Cronin , Michael Charles Grady , David J. Lowe , Brian Michael Roesch , Joseph J. Zaher , Raymond Richard Zolandz
IPC分类号： C12P7/16 , C12P7/14 , C12M1/36 , C12M1/26 , C12M1/34 , C12P7/10 , C12M1/00
CPC分类号： C12P7/16 , C11B1/025 , C11B1/10 , C12M21/12 , C12M33/00 , C12M41/26 , C12M41/48 , C12M43/02 , C12M47/10 , C12P7/10 , C12P7/14 , C12P2203/00 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to the fermentative production of alcohols including ethanol and butanol, and processes for improving alcohol fermentation employing in situ product removal methods.

24. 发明授权

US09580729B2 Method for producing ethanol 有权
标题翻译：乙醇生产方法
公开(公告)号：US09580729B2
公开(公告)日：2017-02-28
申请号：US14385533
申请日：2013-03-21
申请人： Kansai Chemical Engineering Co., Ltd. , Bio-energy Corporation
发明人： Hideo Noda , Shinji Hama , Nobuyuki Kuratani , Akihiko Kondo
IPC分类号： C12P7/10 , C12P7/14
CPC分类号： C12P7/10 , C12P7/14 , Y02E50/16
摘要： A method for producing ethanol from lignocellulosic biomass using yeast at low cost is provided. The method of the present invention for producing ethanol from lignocellulosic biomass includes steps of (1) pretreating lignocellulosic biomass, (2) treating a cellulose fraction obtained in Step (1) with a cellulose hydrolase, (3) mixing saccharified biomass obtained in Step (2) with yeasts to perform ethanol fermentation, and (4) subjecting a fermentation product obtained in Step (3) to a solid-liquid separation, wherein a cycle consisting of Steps (1), (2), (3) and (4) is repeated twice or more, and yeasts obtained in Step (4) are used as all or a portion of yeasts in Step (3) of the subsequent cycle.
摘要翻译：提供了一种使用低成本的酵母从木质纤维素生物质生产乙醇的方法。本发明用于从木质纤维素生物质生产乙醇的方法包括以下步骤：（1）预处理木质纤维素生物质，（2）用纤维素水解酶处理步骤（1）中获得的纤维素级分，（3）将步骤 2）用酵母进行乙醇发酵，和（4）使步骤（3）中获得的发酵产物进行固液分离，其中由步骤（1），（2），（3）和（4）组成的循环）重复两次或更多次，并且步骤（4）中获得的酵母用作随后循环的步骤（3）中的全部或一部分酵母。

25. 发明授权

US09528104B2 System for simultaneous isomerization and fermentation of sugars 有权
标题翻译：糖同时异构化和发酵的系统
公开(公告)号：US09528104B2
公开(公告)日：2016-12-27
申请号：US13955270
申请日：2013-07-31
申请人： The University of Toledo
发明人： Sasidhar Varanasi , Kripa Rao , Patricia Ann Relue , Dawei Yuan
IPC分类号： C12N11/00 , C12N1/18 , C12P7/06
CPC分类号： C12M21/18 , C12M21/12 , C12N1/16 , C12N1/18 , C12N11/00 , C12N11/18 , C12P7/06 , C12P7/14 , Y02E50/17
摘要： Methods and systems for the isomerization and fermentation of xylose and hexose sugars using an immobilized enzyme system capable of sustaining two different pH microenvironments in a single vessel are disclosed. Bilayer particles are dispersed in a mixture comprising an ionic borate source and xylose. The bilayer particles have a first region with a first enzymatic activity comprising xylose isomerase and a pH of 6 or above, and a second region having a second enzymatic activity at an acidic pH.
摘要翻译：公开了使用能够在单个容器中维持两个不同pH微环境的固定化酶系统来异构化和发酵木糖和己糖的方法和系统。双层颗粒分散在包含离子硼酸盐源和木糖的混合物中。双层颗粒具有含有木糖异构酶和pH6以上的第一酶活性的第一区域和在酸性pH下具有第二酶活性的第二区域。

26. 发明授权

US09518281B1 Bioprocessing 有权
公开(公告)号：US09518281B1
公开(公告)日：2016-12-13
申请号：US15232148
申请日：2016-08-09
申请人： XYLECO, INC.
发明人： Marshall Medoff , Thomas Craig Masterman , Harrison Medoff
IPC分类号： C12N11/00 , C12N11/02 , C12N11/08 , C12P19/14 , C12P19/02 , C12N11/14
CPC分类号： C12M45/07 , C12M25/00 , C12N11/00 , C12N11/14 , C12P7/02 , C12P7/06 , C12P7/065 , C12P7/10 , C12P7/14 , C12P19/02 , C12P19/14 , C12P2203/00 , Y02E50/16 , Y02E50/17 , Y02P30/20
摘要： Functionalized substrate materials, for example inorganic particles and/or synthetic polymeric particles, are used to enhance bioprocesses such as saccharification and fermentation.

27. 发明申请

US20160348141A1 BIOPROCESSING 有权
公开(公告)号：US20160348141A1
公开(公告)日：2016-12-01
申请号：US15232148
申请日：2016-08-09
申请人： XYLECO, INC.
发明人： Marshall Medoff , Thomas Craig Masterman , Harrison Medoff
IPC分类号： C12P19/14 , C12N11/14 , C12P19/02
CPC分类号： C12M45/07 , C12M25/00 , C12N11/00 , C12N11/14 , C12P7/02 , C12P7/06 , C12P7/065 , C12P7/10 , C12P7/14 , C12P19/02 , C12P19/14 , C12P2203/00 , Y02E50/16 , Y02E50/17 , Y02P30/20
摘要： Functionalized substrate materials, for example inorganic particles and/or synthetic polymeric particles, are used to enhance bioprocesses such as saccharification and fermentation.

28. 发明授权

US09506049B2 Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same 有权
公开(公告)号：US09506049B2
公开(公告)日：2016-11-29
申请号：US14486729
申请日：2014-09-15
申请人： Novozymes A/S , Novozymes, Inc.
发明人： Lan Tang , Ye Liu , Junxin Duan , Yu Zhang , Christian Joergensen , Randall Kramer
IPC分类号： C12N9/42 , C07K14/385 , C12P7/14 , C12P19/14 , C12N15/01 , C12N15/113 , C12N15/82 , C12P19/02
CPC分类号： C12N9/2437 , C07K14/385 , C12N9/2434 , C12N15/01 , C12N15/1137 , C12N15/8218 , C12N15/8246 , C12N2310/14 , C12N2310/141 , C12P7/14 , C12P19/02 , C12P19/14 , C12P2203/00
摘要： The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

29. 发明授权

US09499839B2 Methods for fermenting carbohydrate-rich crops 有权
标题翻译：发酵碳水化合物丰富的作物的方法
公开(公告)号：US09499839B2
公开(公告)日：2016-11-22
申请号：US14966650
申请日：2015-12-11
申请人： Edward Brian Hamrick
发明人： Edward Brian Hamrick
IPC分类号： C12P7/06 , C12P7/14 , C08B37/00 , C08B30/04
CPC分类号： C12P7/06 , C08B30/04 , C08B37/0003 , C08B37/0054 , C12P7/14 , Y02E50/17
摘要： A method for fermenting carbohydrate-rich crops is provided. Sugar beet, sugar cane, sweet sorghum, tropical maize hybrids and fruits are rich in simple sugars; potato, sweet potato, cassava and yam are rich in starch; and Jerusalem artichoke is rich in inulin. This method uses vacuum infusion to infuse yeast into the intercellular space (apoplast) of the parenchyma tissue. The simple sugars diffuse into the apoplast, come into contact with the yeast and produce ethanol. Ethanol can be extracted from the crop by vacuum stripping or crushing or can be left inside the starchy crop to preserve it. In some variants, pectinase enzymes degrade the parenchyma cell walls to speed up diffusion of simple sugars to the yeast, speed up diffusion of amylase to starch granules or speed up diffusion of inulinase to insoluble inulin.
摘要翻译：提供了一种发酵富含碳水化合物的作物的方法。甜菜，甘蔗，甜高粱，热带玉米杂种和水果富含简单的糖类; 土豆，甘薯，木薯，山药均含淀粉; 而菊芋菊粉富含菊粉。该方法使用真空输注将酵母浸入实质组织的细胞间隙（质外体）中。简单的糖分散入原生质体，与酵母接触并产生乙醇。可以通过真空剥离或破碎从作物中提取乙醇，或者可以留在淀粉作物中以保存。在一些变体中，果胶酶降解薄壁细胞壁以加速单糖向酵母的扩散，加速淀粉酶向淀粉颗粒的扩散，或加速菊粉酶向不溶性菊粉的扩散。

30. 发明授权

US09493802B2 Use of glycohydrolase 61 protein variants with improved thermostability for processing cellulose 有权
标题翻译：使用具有改善热稳定性的糖水解酶61蛋白质变体来加工纤维素
公开(公告)号：US09493802B2
公开(公告)日：2016-11-15
申请号：US13640471
申请日：2011-08-22
申请人： Jie Yang , Louis Clark , Onorato Campopiano , Kripa Rao , Lorand Szabo , Janos Torok , Dipnath Baidyaroy
发明人： Jie Yang , Louis Clark , Onorato Campopiano , Kripa Rao , Lorand Szabo , Janos Torok , Dipnath Baidyaroy
IPC分类号： C12P19/02 , C12N9/30 , C12N9/42 , C12P7/10 , C12P19/14 , C12P7/14
CPC分类号： C12P19/02 , C12N9/242 , C12N9/2434 , C12P7/10 , C12P7/14 , C12P19/14 , Y02E50/16 , Y02P20/52
摘要： The invention provides recombinant GH61 proteins obtained from Myceliophtora thermophila, and nucleic acids that encode such proteins. The invention also provides protein fractions isolated from M. thermophila supernatant that have GH61 protein activity. These preparations can be used to increase yield of products from reactions in which a cellulose-containing substrate undergoes saccharification by one or more cellulase enzymes, such as endoglucanase, β-glucosidase, or cellobiohydrolase. Combinations of GH61 protein and cellulases can be used to break down cellulosic biomass into fermentable sugars in the production of ethanol.
摘要翻译：本发明提供了从嗜热丝霉菌（Myceliophtora thermophila）获得的重组GH61蛋白质和编码这种蛋白质的核酸。本发明还提供从具有GH61蛋白活性的嗜热绵羊嗜热上清液分离的蛋白质级分。可以使用这些制剂从含有纤维素的底物通过一种或多种纤维素酶如内切葡聚糖酶，β-葡糖苷酶或纤维二糖水解酶进行糖化的反应来提高产品的产量。 GH61蛋白和纤维素酶的组合可用于在生产乙醇中将纤维素生物质分解成可发酵糖。

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