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1. 发明申请

US20160201092A1 METHOD FOR PRODUCING ETHANOL 有权
标题翻译：生产乙醇的方法
公开(公告)号：US20160201092A1
公开(公告)日：2016-07-14
申请号：US14915948
申请日：2014-09-03
申请人： KANSAI CHEMICAL ENGINEERING CO., LTD. , BIO-ENERGY CORPORATION
发明人： Hideo NODA , Shinji HAMA , Kohsuke NAKANO
IPC分类号： C12P7/10
CPC分类号： C12P7/10 , Y02E50/16
摘要： Disclosed is a method for producing ethanol, including: culturing yeast transformed so as to display an enzyme on the cell surface in a medium containing particles of lignocellulosic biomass, thereby producing ethanol, wherein the enzyme is an enzyme involved in hydrolysis of the lignocellulosic biomass. The present invention makes it possible to provide a method for producing ethanol by which a high ethanol yield can be achieved from lignocellulosic biomass with lower initial cell concentration and added enzyme amount.
摘要翻译：公开了一种生产乙醇的方法，包括：在含有木质纤维素生物质颗粒的培养基中培养转化酵母以在细胞表面显示酵母，由此生产乙醇，其中酶是参与木质纤维素生物质水解的酶。本发明使得可以提供一种生产乙醇的方法，通过该方法可以从具有较低初始细胞浓度和添加的酶量的木质纤维素生物质获得高乙醇产率。

2. 发明授权

US09816113B2 Method for producing ethanol 有权
公开(公告)号：US09816113B2
公开(公告)日：2017-11-14
申请号：US14915948
申请日：2014-09-03
申请人： Kansai Chemical Engineering Co., Ltd. , Bio-energy Corporation
发明人： Hideo Noda , Shinji Hama , Kohsuke Nakano
IPC分类号： C12P7/10 , C12P7/06 , C12N9/00 , C12N9/14 , C12N9/16 , C12N9/24 , C12N9/26 , C12N9/34 , C12N9/38 , C12N9/44 , C12N9/46 , C12N1/00
CPC分类号： C12P7/10 , Y02E50/16
摘要： Disclosed is a method for producing ethanol, including: culturing yeast transformed so as to display an enzyme on the cell surface in a medium containing particles of lignocellulosic biomass, thereby producing ethanol, wherein the enzyme is an enzyme involved in hydrolysis of the lignocellulosic biomass. The present invention makes it possible to provide a method for producing ethanol by which a high ethanol yield can be achieved from lignocellulosic biomass with lower initial cell concentration and added enzyme amount.

3. 发明申请

US20150284748A1 CONTINUOUS PRODUCTION OF BIODIESEL FUEL BY ENZYMATIC METHOD 有权
标题翻译：通过酶联免疫法连续生产生物柴油
公开(公告)号：US20150284748A1
公开(公告)日：2015-10-08
申请号：US14741755
申请日：2015-06-17
申请人： Kansai Chemical Engineering Co., Ltd. , Bio-energy Corporation
发明人： Nobuyuki Kuratani , Shinji Hama , Hideo Noda , Hideki Fukuda
IPC分类号： C12P7/64
CPC分类号： C12P7/649 , C10G2300/1011 , C11C3/003 , C12P7/62 , Y02E50/13 , Y02P30/20
摘要： A method for continuously producing a fatty acid ester of the present invention comprises (a) mixing and agitating an oil and fat starting material and a lower alcohol, and supplying a mixture to one of the catalyst reaction tubes filled with a lipase; (b) producing a fatty acid ester and glycerin in the catalyst reaction tube; (c) introducing an outflowing liquid from the catalyst reaction tube into a glycerin separation tank, thereby collecting the glycerin; (d) adding a lower alcohol to a separated liquid obtained by separating the glycerin from the outflowing liquid, mixing and agitating an obtained material, and supplying a mixture to a following catalyst reaction tube; (e) repeating the steps (b) to (d) until supply to a last catalyst reaction tube is performed; and (f) collecting a fatty acid ester from the separated liquid obtained from the last catalyst reaction tube. According to the method of the present invention, the concentration of a lower alcohol can be strictly controlled and by-product glycerin can be automatically removed.
摘要翻译：本发明的连续生产脂肪酸酯的方法包括：（a）将油脂原料和低级醇混合搅拌，向填充有脂肪酶的催化剂反应管之一供给混合物; （b）在催化剂反应管中制备脂肪酸酯和甘油; （c）将来自催化剂反应管的流出液引入甘油分离槽中，从而收集甘油; （d）向从流出液中分离甘油得到的分离液中加入低级醇，混合搅拌所得物质，向后述催化剂反应管供给混合物; （e）重复步骤（b）至（d），直到进行最后一个催化剂反应管的供应; 和（f）从最后一个催化剂反应管获得的分离液中收集脂肪酸酯。根据本发明的方法，可以严格控制低级醇的浓度，并可以自动除去副产物甘油。

4. 发明授权

US11306300B2 Microorganism capable of displaying α-galactosidase on surface layer thereof, and use thereof 有权
公开(公告)号：US11306300B2
公开(公告)日：2022-04-19
申请号：US16607768
申请日：2018-05-10
申请人： Kansai Chemical Engineering Co., Ltd. , Bio-energy Corporation
发明人： Hideo Noda , Shinji Hama
IPC分类号： C12N9/40 , C12N15/81 , C12P7/06 , C12P7/56 , C12P19/02 , C12P19/14
摘要： The present invention provides a transformed microorganism capable of displaying α-galactosidase on its surface layer. Also provided is a method for producing an alcohol, which includes the step of culturing the transformed microorganism in a culture medium containing a material that contains an oligosaccharide α-1,6 linked α-galactose. Also provided is a method for producing lactic acid using such a transformed microorganism together with a material that contains an oligosaccharide α-1,6 linked α-galactose. According to the present invention, a microorganism can be provided, which can degrade an oligosaccharide containing α-1,6 linked α-galactose, which may occur in soybean molasses.

5. 发明授权

US09879291B2 Continuous production of biodiesel fuel by enzymatic method 有权
公开(公告)号：US09879291B2
公开(公告)日：2018-01-30
申请号：US14741755
申请日：2015-06-17
申请人： Kansai Chemical Engineering Co., Ltd. , Bio-energy Corporation
发明人： Nobuyuki Kuratani , Shinji Hama , Hideo Noda , Hideki Fukuda
IPC分类号： C12P7/62 , C12P7/64 , C11C3/00
CPC分类号： C12P7/649 , C10G2300/1011 , C11C3/003 , C12P7/62 , Y02E50/13 , Y02P30/20
摘要： A method for continuously producing a fatty acid ester of the present invention comprises (a) mixing and agitating an oil and fat starting material and a lower alcohol, and supplying a mixture to one of the catalyst reaction tubes filled with a lipase; (b) producing a fatty acid ester and glycerin in the catalyst reaction tube; (c) introducing an outflowing liquid from the catalyst reaction tube into a glycerin separation tank, thereby collecting the glycerin; (d) adding a lower alcohol to a separated liquid obtained by separating the glycerin from the outflowing liquid, mixing and agitating an obtained material, and supplying a mixture to a following catalyst reaction tube; (e) repeating the steps (b) to (d) until supply to a last catalyst reaction tube is performed; and (f) collecting a fatty acid ester from the separated liquid obtained from the last catalyst reaction tube. According to the method of the present invention, the concentration of a lower alcohol can be strictly controlled and by-product glycerin can be automatically removed.

6. 发明申请

US20150037858A1 METHOD FOR PRODUCING ETHANOL 有权
标题翻译：生产乙醇的方法
公开(公告)号：US20150037858A1
公开(公告)日：2015-02-05
申请号：US14385533
申请日：2013-03-21
申请人： Kansai Chemical Engineering Co., Ltd. , Bio-energy Corporation
发明人： Hideo Noda , Shinji Hama , Nobuyuki Kuratani , Akihiko Kondo
IPC分类号： C12P7/10
CPC分类号： C12P7/10 , C12P7/14 , Y02E50/16
摘要： A method for producing ethanol from lignocellulosic biomass using yeast at low cost is provided. The method of the present invention for producing ethanol from lignocellulosic biomass includes steps of (1) pretreating lignocellulosic biomass, (2) treating a cellulose fraction obtained in Step (1) with a cellulose hydrolase, (3) mixing saccharified biomass obtained in Step (2) with yeasts to perform ethanol fermentation, and (4) subjecting a fermentation product obtained in Step (3) to a solid-liquid separation, wherein a cycle consisting of Steps (1), (2), (3) and (4) is repeated twice or more, and yeasts obtained in Step (4) are used as all or a portion of yeasts in Step (3) of the subsequent cycle.
摘要翻译：提供了一种使用低成本的酵母从木质纤维素生物质生产乙醇的方法。本发明用于从木质纤维素生物质生产乙醇的方法包括以下步骤：（1）预处理木质纤维素生物质，（2）用纤维素水解酶处理步骤（1）中获得的纤维素级分，（3）将步骤 2）用酵母进行乙醇发酵，和（4）使步骤（3）中获得的发酵产物进行固液分离，其中由步骤（1），（2），（3）和（4）组成的循环）重复两次或更多次，并且步骤（4）中获得的酵母作为随后循环的步骤（3）中的全部或一部分酵母使用。

7. 发明申请

US20210284984A1 MICROORGANISM CAPABLE OF DISPLAYING a-GALACTOSIDASE ON SURFACE LAYER THEREOF, AND USE THEREOF 有权
公开(公告)号：US20210284984A1
公开(公告)日：2021-09-16
申请号：US16607768
申请日：2018-05-10
申请人： Kansai Chemical Engineering Co., Ltd. , Bio-energy Corporation
发明人： HIDEO NODA , SHINJI HAMA
IPC分类号： C12N9/40 , C12N15/81 , C12P7/06 , C12P19/02 , C12P19/14 , C12P7/56
摘要： The present invention provides a transformed microorganism capable of displaying α-galactosidase on its surface layer. Also provided is a method for producing an alcohol, which includes the step of culturing the transformed microorganism in a culture medium containing a material that contains an oligosaccharide α-1,6 linked α-galactose. Also provided is a method for producing lactic acid using such a transformed microorganism together with a material that contains an oligosaccharide α-1,6 linked α-galactose. According to the present invention, a microorganism can be provided, which can degrade an oligosaccharide containing α-1,6 linked α-galactose, which may occur in soybean molasses.

8. 发明申请

US20180216143A1 METHOD FOR PRODUCING FATTY ACID ESTER 审中-公开
公开(公告)号：US20180216143A1
公开(公告)日：2018-08-02
申请号：US15552111
申请日：2016-07-01
申请人： KANSAI CHEMICAL ENGINEERING CO., LTD. , BIO-ENERGY CORPORATION
发明人： AYUMI YOSHIDA , SHINJI HAMA , HIDEO NODA
IPC分类号： C12P7/64 , C12P7/62 , C11C3/00
CPC分类号： C12P7/6436 , C11C3/003 , C12P7/62 , Y02E50/13
摘要： A method for producing a fatty acid ester according to the present invention includes mixing a raw fat or oil, a liquid enzyme, and an alcohol having 1 to 8 carbon atoms in the presence of water and an electrolyte. According to the present invention, a fatty acid ester can be efficiently produced via a transesterification reaction without using any expensive buffer solution or amphipathic substance. Furthermore, in the reaction, it is not necessarily required to agitate reactants at a high speed, which makes the present invention adaptive to various production facilities and conditions.

9. 发明授权

US10781464B2 Method for producing fatty acid ester 有权
公开(公告)号：US10781464B2
公开(公告)日：2020-09-22
申请号：US15552111
申请日：2016-07-01
申请人： KANSAI CHEMICAL ENGINEERING CO., LTD. , BIO-ENERGY CORPORATION
发明人： Ayumi Yoshida , Shinji Hama , Hideo Noda
IPC分类号： C12P7/64 , C12P7/62 , C11C3/00 , C12N9/20
摘要： A method for producing a fatty acid ester according to the present invention includes mixing a raw fat or oil, a liquid enzyme, and an alcohol having 1 to 8 carbon atoms in the presence of water and an electrolyte. According to the present invention, a fatty acid ester can be efficiently produced via a transesterification reaction without using any expensive buffer solution or amphipathic substance. Furthermore, in the reaction, it is not necessarily required to agitate reactants at a high speed, which makes the present invention adaptive to various production facilities and conditions.

10. 发明授权

US09580729B2 Method for producing ethanol 有权
标题翻译：乙醇生产方法
公开(公告)号：US09580729B2
公开(公告)日：2017-02-28
申请号：US14385533
申请日：2013-03-21
申请人： Kansai Chemical Engineering Co., Ltd. , Bio-energy Corporation
发明人： Hideo Noda , Shinji Hama , Nobuyuki Kuratani , Akihiko Kondo
IPC分类号： C12P7/10 , C12P7/14
CPC分类号： C12P7/10 , C12P7/14 , Y02E50/16
摘要： A method for producing ethanol from lignocellulosic biomass using yeast at low cost is provided. The method of the present invention for producing ethanol from lignocellulosic biomass includes steps of (1) pretreating lignocellulosic biomass, (2) treating a cellulose fraction obtained in Step (1) with a cellulose hydrolase, (3) mixing saccharified biomass obtained in Step (2) with yeasts to perform ethanol fermentation, and (4) subjecting a fermentation product obtained in Step (3) to a solid-liquid separation, wherein a cycle consisting of Steps (1), (2), (3) and (4) is repeated twice or more, and yeasts obtained in Step (4) are used as all or a portion of yeasts in Step (3) of the subsequent cycle.
摘要翻译：提供了一种使用低成本的酵母从木质纤维素生物质生产乙醇的方法。本发明用于从木质纤维素生物质生产乙醇的方法包括以下步骤：（1）预处理木质纤维素生物质，（2）用纤维素水解酶处理步骤（1）中获得的纤维素级分，（3）将步骤 2）用酵母进行乙醇发酵，和（4）使步骤（3）中获得的发酵产物进行固液分离，其中由步骤（1），（2），（3）和（4）组成的循环）重复两次或更多次，并且步骤（4）中获得的酵母用作随后循环的步骤（3）中的全部或一部分酵母。

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