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21. 发明授权

US07960530B2 Fluorescent protein 有权
标题翻译：荧光蛋白
公开(公告)号：US07960530B2
公开(公告)日：2011-06-14
申请号：US10561040
申请日：2004-06-16
申请人： Atsushi Miyawaki , Hidekazu Tsutsui , Satoshi Karasawa
发明人： Atsushi Miyawaki , Hidekazu Tsutsui , Satoshi Karasawa
IPC分类号： C07H21/04 , C12P21/06
CPC分类号： C07K14/43595
摘要： An object of the present invention is to provide a novel fluorescent protein derived from favia favus. The present invention provides a fluorescent protein derived from favia favus having the following properties: (1) an excitation maximum wavelength is 507 nm; (2) a fluorescence maximum wavelength is 517 nm; (3) a molar absorption coefficient at 482 nm is 80,000; (4) a quantum yield is 0.68; and (5) pH sensitivity of the fluorescence maximum is stable at pH 5 to pH 11.
摘要翻译：本发明的一个目的是提供一种来源于粉刺的新型荧光蛋白。本发明提供具有以下特性的源自粉刺的荧光蛋白：（1）激发最大波长为507nm; （2）荧光最大波长为517nm; （3）在482nm处的摩尔吸光系数为80,000; （4）量子产率为0.68; 和（5）荧光最大的pH敏感性在pH5至pH11下是稳定的。

22. 发明授权

US07897385B2 Fluorescent protein 有权
公开(公告)号：US07897385B2
公开(公告)日：2011-03-01
申请号：US11569275
申请日：2005-05-20
申请人： Atsushi Miyawaki , Ryoko Ando , Hideaki Mizuno , Satoshi Karasawa
发明人： Atsushi Miyawaki , Ryoko Ando , Hideaki Mizuno , Satoshi Karasawa
IPC分类号： C12N5/00 , C07K14/00 , C07H21/04
CPC分类号： C07K14/43595 , G03C1/731 , G03C1/733
摘要： The object of the present invention is to provide a novel fluorescent protein in which on and off of fluorescence thereof can be controlled by irradiation with lights of two different wavelengths. The present invention provides a fluorescent protein shown in the following (a) or (b); (a) a protein which has the amino acid sequence shown in SEQ ID NO: 3 or 7; or (b) a protein which has an amino acid sequence comprising a deletion, substitution, and/or addition of one or several amino acids with respect to the amino acid sequence shown in SEQ ID NO: 3 or 7, and which has fluorescence characteristics of exhibiting a photochromic effect.

23. 发明申请

US20090170073A1 FLUORESCENT PROTEIN AND CHROMOPROTEIN 失效
标题翻译：荧光蛋白和色氨酸蛋白
公开(公告)号：US20090170073A1
公开(公告)日：2009-07-02
申请号：US10561041
申请日：2004-06-16
申请人： Atsushi Miyawaki , Hidekazu Tsutsui , Satoshi Karasawa
发明人： Atsushi Miyawaki , Hidekazu Tsutsui , Satoshi Karasawa
IPC分类号： C12Q1/68 , C07K2/00 , C07H21/04
CPC分类号： C07K14/43504
摘要： It is an object of the present invention to provide a novel fluorescent protein and a novel chromoprotein. The present invention provides a novel fluorescent protein derived from Montipora sp., Acropora sp. and Lobophytum crassum, and a novel chromoprotein derived from Actinia equine.
摘要翻译：本发明的目的是提供一种新的荧光蛋白和新的色蛋白。本发明提供了一种新型的从Montipora sp。，Acropora sp。和Lobophytum crassum，以及衍生自猕猴桃的新型染色体蛋白。

24. 发明申请

US20080227097A1 FLUORESCENT PROTEIN AND CHROMOPROTEIN 失效
标题翻译：荧光蛋白和色氨酸蛋白
公开(公告)号：US20080227097A1
公开(公告)日：2008-09-18
申请号：US12016903
申请日：2008-01-18
申请人： Atsushi MIYAWAKI , Ryoko Ando , Satoshi Karasawa , Hideaki Mizuno
发明人： Atsushi MIYAWAKI , Ryoko Ando , Satoshi Karasawa , Hideaki Mizuno
IPC分类号： C12Q1/68 , C07H21/04 , C12N15/00 , C12N1/20 , C07K16/00
CPC分类号： G01N33/542 , C07K14/37 , C07K2319/00 , G01N33/533 , G01N33/582 , G01N2021/6439 , G01N2500/10
摘要： It is an object of the present invention to provide a novel chromoprotein and a novel fluorescent protein. The present invention provides chromoproteins derived from Anthopleura inornata, which have certain property, and fluorescent proteins from Trachyphyllia geoffroyi and Scolymia vitiensis, which have certain fluorescent property.
摘要翻译：本发明的目的是提供一种新型的生色蛋白和新的荧光蛋白。本发明提供了具有一定性质的Anthopleura inornata衍生的色蛋白，以及具有一定荧光性质的来自Trachyphyllia geoffroyi和Scolymia vitiensis的荧光蛋白。

25. 发明授权

US07375201B2 Fluorescent protein 失效
标题翻译：荧光蛋白
公开(公告)号：US07375201B2
公开(公告)日：2008-05-20
申请号：US10504325
申请日：2003-02-25
申请人： Atsushi Miyawaki , Ryoko Ando , Hidetomo Tanase , Satoshi Karasawa
发明人： Atsushi Miyawaki , Ryoko Ando , Hidetomo Tanase , Satoshi Karasawa
IPC分类号： C07H21/04 , C12N15/00 , C12N5/00
CPC分类号： C07K14/43595
摘要： An object of the present invention is to provide a fluorescent protein derived from organisms other than Aequorea victoria and having a novel primary structure. According to the present invention, there is provided a fluorescent protein derived from Halcurias sp. L, which has the following properties: (1) the excitation maximum wavelength is 494 nm, and the fluorescence maximum wavelength is 506 nm; (2) the molar absorption coefficient at 494 nm is 94600 M−1 cm−1; (3) the quantum yield is 0.65; and (4) the pH sensitivity of the fluorescent property is low in the range between pH 4 and pH 11.
摘要翻译：本发明的目的是提供一种衍生自维他命A以外的生物体并具有新一级结构的荧光蛋白。根据本发明，提供了衍生自Halcurias sp。 L具有以下性质：（1）激发最大波长为494nm，荧光最大波长为506nm; （2）在494nm处的摩尔吸光系数为94600M -1 -1 cm -1; （3）量子产率为0.65; 和（4）pH4和pH11之间的荧光特性的pH敏感性低。

26. 发明申请

US20070031912A1 Method for analysis of protein interaction using fluorescent protein 审中-公开
标题翻译：使用荧光蛋白分析蛋白质相互作用的方法
公开(公告)号：US20070031912A1
公开(公告)日：2007-02-08
申请号：US11390215
申请日：2006-03-28
申请人： Atsushi Miyawaki , Satoshi Karasawa
发明人： Atsushi Miyawaki , Satoshi Karasawa
IPC分类号： C12Q1/37
CPC分类号： G01N33/542
摘要： An object of the present invention is to provide a method for analyzing the protein interaction, wherein the information about time can be obtained and the movement of protein can be monitored. The present invention provides a method for analyzing interaction between a first test protein and a second test protein which comprises the steps of: splitting a fluorescent protein capable of emitting different color of fluorescence according to passage of time into an N-terminal fragment and a C-terminal fragment; allowing the first test protein to interact with the second test protein by making coexist a fusion protein of the N-terminal fragment with the first test protein and another fusion protein of the C-terminal fragment with the second test protein; and detecting the change in the fluorescent light due to the interaction.
摘要翻译：本发明的目的是提供一种用于分析蛋白质相互作用的方法，其中可以获得关于时间的信息，并且可以监测蛋白质的移动。本发明提供了一种用于分析第一测试蛋白质和第二测试蛋白质之间的相互作用的方法，其包括以下步骤：将能够根据时间流逝发射不同颜色的荧光的荧光蛋白分成N末端片段和C 末端片段通过使N末端片段的融合蛋白与第一测试蛋白和C末端片段的另一融合蛋白与第二测试蛋白共存，允许第一测试蛋白与第二测试蛋白相互作用; 并且检测由于相互作用引起的荧光灯的变化。

27. 发明授权

US08420781B2 Fluorescent protein 有权
标题翻译：荧光蛋白
公开(公告)号：US08420781B2
公开(公告)日：2013-04-16
申请号：US13196499
申请日：2011-08-02
申请人： Atsushi Miyawaki , Takako Kogure , Hiroshi Hama , Masataka Kinjo , Kenta Saito , Satoshi Karasawa , Toshio Araki
发明人： Atsushi Miyawaki , Takako Kogure , Hiroshi Hama , Masataka Kinjo , Kenta Saito , Satoshi Karasawa , Toshio Araki
IPC分类号： C07K1/00 , C08B37/08
CPC分类号： C07K14/37 , C07K14/43504 , G01N33/533
摘要： An object of the present invention is to provide a red or orange fluorescent protein, which is characterized in that the difference (stokes shift) between an excitation peak value (wavelength of maximum absorption) and a fluorescence peak value (wavelength of maximum fluorescence) is greatened, so that the maximum fluorescence can be obtained by the maximum excitation. The present invention provides a novel fluorescent protein monomerized by introducing a mutation into a florescent protein derived from Fungia sp., and a novel chromoprotein and fluorescent protein derived from Montipora. sp.
摘要翻译：本发明的目的是提供一种红色或橙色荧光蛋白，其特征在于，激发峰值（最大吸收波长）与荧光峰值（最大荧光的波长）之间的差异（斯托克斯偏移）为从而可以通过最大激发获得最大荧光。本发明提供了一种新颖的荧光蛋白，其通过将突变引入到来自真菌属的荧光蛋白中而单体化，以及衍生自Montipora的新型生色蛋白和荧光蛋白。 sp。

28. 发明授权

US08346036B2 Confocal optical system 有权
公开(公告)号：US08346036B2
公开(公告)日：2013-01-01
申请号：US12776525
申请日：2010-05-10
申请人： Satoshi Karasawa
发明人： Satoshi Karasawa
IPC分类号： G02B6/32
摘要： A confocal optical system comprising a scanning fiber is provided. The scanning fiber is a single-mode fiber of which a first end is shaped as a curved surface. The scanning fiber transmits illumination light to the first end. The illumination light is emitted toward an observation area. The illumination light emanates from the first end. The illumination light emanates from the first end striking a target area within the observation area. The first end receives at least one of reflected light and fluorescence from the target area. The reflected light is the illumination light reflected from the target area. The fluorescence is induced at the target area by illumination from the illumination light.

29. 发明授权

US08017746B2 Fluorescent protein 有权
标题翻译：荧光蛋白
公开(公告)号：US08017746B2
公开(公告)日：2011-09-13
申请号：US10581551
申请日：2004-12-03
申请人： Atsushi Miyawaki , Takako Kogure , Hiroshi Hama , Masataka Kinjo , Kenta Saito , Satoshi Karasawa , Toshio Araki
发明人： Atsushi Miyawaki , Takako Kogure , Hiroshi Hama , Masataka Kinjo , Kenta Saito , Satoshi Karasawa , Toshio Araki
IPC分类号： C07H21/02 , C12Q1/02 , C12N1/20
CPC分类号： C07K14/37 , C07K14/43504 , G01N33/533
摘要： An object of the present invention is to provide a red or orange fluorescent protein, which is characterized in that the difference (stokes shift) between an excitation peak value (wavelength of maximum absorption) and a fluorescence peak value (wavelength of maximum fluorescence) is greatened, so that the maximum fluorescence can be obtained by the maximum excitation. The present invention provides a novel fluorescent protein monomerized by introducing a mutation into a florescent protein derived from Fungia sp., and a novel chromoprotein and fluorescent protein derived from Montipora. sp.
摘要翻译：本发明的目的是提供一种红色或橙色荧光蛋白，其特征在于，激发峰值（最大吸收波长）与荧光峰值（最大荧光的波长）之间的差异（斯托克斯偏移）为从而可以通过最大激发获得最大荧光。本发明提供了一种新颖的荧光蛋白，其通过将突变引入到来自真菌属的荧光蛋白中而单体化，以及衍生自Montipora的新型生色蛋白和荧光蛋白。 sp。

30. 发明授权

US07547528B2 Fluorescent protein and chromoprotein 失效
标题翻译：荧光蛋白和荧光蛋白
公开(公告)号：US07547528B2
公开(公告)日：2009-06-16
申请号：US12016903
申请日：2008-01-18
申请人： Atsushi Miyawaki , Ryoko Ando , Satoshi Karasawa , Hideaki Mizuno
发明人： Atsushi Miyawaki , Ryoko Ando , Satoshi Karasawa , Hideaki Mizuno
IPC分类号： C12P21/06 , C12N15/00 , C12N9/20 , C07K1/00
CPC分类号： G01N33/542 , C07K14/37 , C07K2319/00 , G01N33/533 , G01N33/582 , G01N2021/6439 , G01N2500/10
摘要： It is an object of the present invention to provide a novel chromoprotein and a novel fluorescent protein. The present invention provides chromoproteins derived from Anthopleura inornata, which have certain property, and fluorescent proteins from Trachyphyllia geoffroyi and Scolymia vitiensis, which have certain fluorescent property.
摘要翻译：本发明的目的是提供一种新型的生色蛋白和新的荧光蛋白。本发明提供了具有一定性质的Anthopleura inornata衍生的色蛋白，以及具有一定荧光性质的来自Trachyphyllia geoffroyi和Scolymia vitiensis的荧光蛋白。

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