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1. 发明申请

US20070292909A1 Fluorescent Protein 有权
标题翻译：荧光蛋白
公开(公告)号：US20070292909A1
公开(公告)日：2007-12-20
申请号：US10581551
申请日：2004-12-03
申请人： Atsushi Miyawaki , Takako Kogure , Hiroshi Hama , Masataka Kinjo , Kenta Saito , Satoshi Karasawa , Toshio Araki
发明人： Atsushi Miyawaki , Takako Kogure , Hiroshi Hama , Masataka Kinjo , Kenta Saito , Satoshi Karasawa , Toshio Araki
IPC分类号： C07H21/04 , C07K14/00 , C12N15/00 , C12Q1/02
CPC分类号： C07K14/37 , C07K14/43504 , G01N33/533
摘要： An object of the present invention is to provide a red or orange fluorescent protein, which is characterized in that the difference (stokes shift) between an excitation peak value (wavelength of maximum absorption) and a fluorescence peak value (wavelength of maximum fluorescence) is greatened, so that the maximum fluorescence can be obtained by the maximum excitation. The present invention provides a novel fluorescent protein monomerized by introducing a mutation into a florescent protein derived from Fungia sp., and a novel chromoprotein and fluorescent protein derived from Montipora. sp.
摘要翻译：本发明的目的是提供一种红色或橙色荧光蛋白，其特征在于，激发峰值（最大吸收波长）与荧光峰值（最大荧光的波长）之间的差异（斯托克斯偏移）为从而可以通过最大激发获得最大荧光。本发明提供了一种新颖的荧光蛋白，其通过将突变引入到来自真菌属的荧光蛋白中而单体化，以及衍生自Montipora的新型生色蛋白和荧光蛋白。 sp。

2. 发明授权

US08420781B2 Fluorescent protein 有权
标题翻译：荧光蛋白
公开(公告)号：US08420781B2
公开(公告)日：2013-04-16
申请号：US13196499
申请日：2011-08-02
申请人： Atsushi Miyawaki , Takako Kogure , Hiroshi Hama , Masataka Kinjo , Kenta Saito , Satoshi Karasawa , Toshio Araki
发明人： Atsushi Miyawaki , Takako Kogure , Hiroshi Hama , Masataka Kinjo , Kenta Saito , Satoshi Karasawa , Toshio Araki
IPC分类号： C07K1/00 , C08B37/08
CPC分类号： C07K14/37 , C07K14/43504 , G01N33/533
摘要： An object of the present invention is to provide a red or orange fluorescent protein, which is characterized in that the difference (stokes shift) between an excitation peak value (wavelength of maximum absorption) and a fluorescence peak value (wavelength of maximum fluorescence) is greatened, so that the maximum fluorescence can be obtained by the maximum excitation. The present invention provides a novel fluorescent protein monomerized by introducing a mutation into a florescent protein derived from Fungia sp., and a novel chromoprotein and fluorescent protein derived from Montipora. sp.
摘要翻译：本发明的目的是提供一种红色或橙色荧光蛋白，其特征在于，激发峰值（最大吸收波长）与荧光峰值（最大荧光的波长）之间的差异（斯托克斯偏移）为从而可以通过最大激发获得最大荧光。本发明提供了一种新颖的荧光蛋白，其通过将突变引入到来自真菌属的荧光蛋白中而单体化，以及衍生自Montipora的新型生色蛋白和荧光蛋白。 sp。

3. 发明授权

US08017746B2 Fluorescent protein 有权
标题翻译：荧光蛋白
公开(公告)号：US08017746B2
公开(公告)日：2011-09-13
申请号：US10581551
申请日：2004-12-03
申请人： Atsushi Miyawaki , Takako Kogure , Hiroshi Hama , Masataka Kinjo , Kenta Saito , Satoshi Karasawa , Toshio Araki
发明人： Atsushi Miyawaki , Takako Kogure , Hiroshi Hama , Masataka Kinjo , Kenta Saito , Satoshi Karasawa , Toshio Araki
IPC分类号： C07H21/02 , C12Q1/02 , C12N1/20
CPC分类号： C07K14/37 , C07K14/43504 , G01N33/533
摘要： An object of the present invention is to provide a red or orange fluorescent protein, which is characterized in that the difference (stokes shift) between an excitation peak value (wavelength of maximum absorption) and a fluorescence peak value (wavelength of maximum fluorescence) is greatened, so that the maximum fluorescence can be obtained by the maximum excitation. The present invention provides a novel fluorescent protein monomerized by introducing a mutation into a florescent protein derived from Fungia sp., and a novel chromoprotein and fluorescent protein derived from Montipora. sp.
摘要翻译：本发明的目的是提供一种红色或橙色荧光蛋白，其特征在于，激发峰值（最大吸收波长）与荧光峰值（最大荧光的波长）之间的差异（斯托克斯偏移）为从而可以通过最大激发获得最大荧光。本发明提供了一种新颖的荧光蛋白，其通过将突变引入到来自真菌属的荧光蛋白中而单体化，以及衍生自Montipora的新型生色蛋白和荧光蛋白。 sp。

4. 发明授权

US10444124B2 Clarifying reagent for biological materials and use thereof 有权
公开(公告)号：US10444124B2
公开(公告)日：2019-10-15
申请号：US14118150
申请日：2012-05-18
申请人： Atsushi Miyawaki , Hiroshi Hama , Asako Sakaue
发明人： Atsushi Miyawaki , Hiroshi Hama , Asako Sakaue
IPC分类号： G01N1/30 , G01N33/50
摘要： A clearing reagent according to the present invention for making a biological material transparent is a solution containing: at a concentration of 1M or more and not more than 8.5M, at least one compound selected from the group consisting of urea and urea derivatives; and glycerol at a concentration of 25 (w/v) % or more and not more than 35 (w/v) %.

5. 发明申请

US20140178927A1 CLARIFYING REAGENT FOR BIOLOGICAL MATERIALS AND USE THEREOF 审中-公开
标题翻译：用于生物材料的澄清试剂及其用途
公开(公告)号：US20140178927A1
公开(公告)日：2014-06-26
申请号：US14118150
申请日：2012-05-18
申请人： Atsushi Miyawaki , Hiroshi Hama , Asako Sakaue
发明人： Atsushi Miyawaki , Hiroshi Hama , Asako Sakaue
IPC分类号： G01N1/30
CPC分类号： G01N1/30 , G01N33/5002 , G01N33/5005 , G01N33/5008 , G01N2001/302
摘要： A clearing reagent according to the present invention for making a biological material transparent is a solution containing: at a concentration of 1M or more and not more than 8.5M, at least one compound selected from the group consisting of urea and urea derivatives; and glycerol at a concentration of 25 (w/v) % or more and not more than 35 (w/v) %.
摘要翻译：根据本发明的用于使生物材料透明的清洗试剂是含有浓度为1M以上至不超过8.5M的至少一种选自脲和脲衍生物的化合物的溶液; 和浓度为25（w / v）％以上且不高于35（w / v）％的甘油。

6. 发明申请

US20130045503A1 CLEARING REAGENT FOR BIOLOGICAL MATERIAL, AND USE THEREOF 审中-公开
标题翻译：生物材料清除试剂及其用途
公开(公告)号：US20130045503A1
公开(公告)日：2013-02-21
申请号：US13583548
申请日：2011-03-11
申请人： Atsushi Miyawaki , Hiroshi Hama , Hiroshi Kurokawa , Hiroyuki Kawano , Asako Sakaue
发明人： Atsushi Miyawaki , Hiroshi Hama , Hiroshi Kurokawa , Hiroyuki Kawano , Asako Sakaue
IPC分类号： G01N1/30
CPC分类号： G01N1/30
摘要： A clearing reagent according to this invention for making a biological material transparent is a solution containing, as an active component, at least one compound selected from the group consisting of urea and urea derivatives, in order to provide a novel clearing reagent for making a biological material transparent.
摘要翻译：根据本发明的用于使生物材料透明化的清洗试剂是含有选自脲和脲衍生物的至少一种化合物作为活性组分的溶液，以提供用于制备生物材料的新型清除试剂材料透明。

7. 发明申请

US20140087419A1 METHOD FOR MAKING BIOLOGICAL MATERIAL TRANSPARENT AND USE THEREOF 审中-公开
标题翻译：制备生物材料透明的方法及其用途
公开(公告)号：US20140087419A1
公开(公告)日：2014-03-27
申请号：US14113639
申请日：2012-04-27
申请人： Atsushi Miyawaki , Hiroshi Hama , Hiroshi Kurokawa
发明人： Atsushi Miyawaki , Hiroshi Hama , Hiroshi Kurokawa
IPC分类号： G01N1/30
摘要： A method for making a biological material transparent according to the present invention includes: a first permeation step of causing a first solution to permeate into a biological material, the first solution containing at least one compound selected from the group consisting of urea and urea derivatives at a predetermined concentration; and then a second permeation step of causing a second solution to permeate into the biological material, the second solution containing at least one compound selected from the group consisting of urea and urea derivatives at a concentration higher than the concentration of the at least one compound contained in the first solution.
摘要翻译：根据本发明的制造生物材料透明的方法包括：使第一溶液渗透到生物材料中的第一渗透步骤，所述第一溶液含有至少一种选自脲和脲衍生物的化合物预定浓度; 然后使第二溶液渗透到生物材料中的第二渗透步骤，所述第二溶液含有至少一种选自脲和脲衍生物的化合物，其浓度高于所含的至少一种化合物的浓度在第一个解决方案。

8. 发明授权

US5708181A Spiropyran compound 失效
标题翻译：螺吡喃化合物
公开(公告)号：US5708181A
公开(公告)日：1998-01-13
申请号：US532802
申请日：1995-11-08
申请人： Hiroshi Hama , Shinji Nakano
发明人： Hiroshi Hama , Shinji Nakano
IPC分类号： C07D517/10 , C09K9/02
CPC分类号： C07D517/10 , C09K9/02
摘要： An object of the present invention is to provide a novel compound which is suitably usable as a photochromic material. The compound of the present invention is a salt of a spiropyran compound and an acidic compound, the spiropyran compound being represented by the formula ##STR1## wherein R.sup.1 means a C.sub.1 to C.sub.20 alkyl group, an aralkyl group, a hydroxyethyl group, an acryloxyethyl group or a methacryloxyethyl group, R.sup.2 to R.sup.7 each mean a hydrogen atom, a C.sub.1 to C.sub.6 alkyl group or the like, R.sup.8 means a hydrogen atom, a vinyl group, a group --CH.sub.2 OR.sup.9 or a group --CH.sub.2 OCOC(R.sup.10).dbd.CH.sub.2 (wherein R.sup.9 means a hydrogen atom or a C.sub.1 to C.sub.4 alkyl group and R.sup.10 means a hydrogen atom or a methyl group), and X means an oxygen atom or a sulfur atom.
摘要翻译： PCT No.PCT / JP95 / 00396 Sec。 371日期：1995年11月8日 102（e）日期1995年11月8日PCT 1995年3月10日PCT PCT。公开号WO95 / 24409 日期1995年9月14日本发明的目的是提供适合用作光致变色材料的新型化合物。本发明化合物是螺吡喃化合物和酸性化合物的盐，螺吡喃化合物由下式表示：其中R1表示C1至C20烷基，芳烷基，羟乙基，丙烯酰氧基乙基或甲基丙烯酰氧基乙基，R2〜R7各自表示氢原子，C1〜C6烷基等，R8表示氢原子，乙烯基，-CH2OR9基或-CH2OCOC（R10）= CH2（其中 R9表示氢原子或C1〜C4烷基，R10表示氢原子或甲基），X表示氧原子或硫原子。

9. 发明申请

US20070080319A1 Rust preventive for magnesium and/or magnesium alloy 审中-公开
标题翻译：镁和/或镁合金防锈剂
公开(公告)号：US20070080319A1
公开(公告)日：2007-04-12
申请号：US10578920
申请日：2003-11-10
申请人： Kazunori Fukumura , Yuhua Yu , Masaya Kajimoto , Hiroshi Hama , Takashi Hamauzu , Hiroshi Yagi
发明人： Kazunori Fukumura , Yuhua Yu , Masaya Kajimoto , Hiroshi Hama , Takashi Hamauzu , Hiroshi Yagi
IPC分类号： C09K5/00
CPC分类号： C23C22/83 , C23F11/149 , C23F11/161
摘要： A corrosion inhibitor for magnesium and/or magnesium alloy containing, as an effective component, a least one kind of compound selected from the group consisting of a compound of the formula (1) and a compound of the formula (2) and salts thereof, and a process for producing components or parts made of magnesium and/or magnesium alloy using the corrosion inhibitor wherein R1 is a hydrogen atom, or C1-4 alkyl, R2 is a hydrogen atom, C1-4 alkyl, mercapto or hydroxy, R3 is a hydrogen atom, C1-4 alkyl or hydroxy, A is —N═ or —C(R4)═, R4 is a hydrogen atom or amino.
摘要翻译：一种用于镁和/或镁合金的腐蚀抑制剂，其包含选自由式（1）的化合物和式（2）的化合物及其盐组成的组中的至少一种化合物作为有效成分，以及使用腐蚀抑制剂制造由镁和/或镁合金制成的部件或部件的方法，其中R 1是氢原子或C 1-4烷基，R H 2是氢原子，C 1-4烷基，巯基或羟基，R 3是氢原子，C 1〜 4个烷基或羟基，A是-N-或-C（R 4） - ，R 4是氢原子或氨基。

10. 发明授权

US5476913A Polymerizable monomer, polymer thereof and process for preparing same 失效
标题翻译：可聚合单体，聚合物及其制备方法
公开(公告)号：US5476913A
公开(公告)日：1995-12-19
申请号：US204210
申请日：1994-07-11
申请人： Hiroki Kourai , Michio Sasaoka , Mitsuo Akada , Yoshio Yabuhara , Kouji Mori , Akihiro Murakami , Hiroshi Hama
发明人： Hiroki Kourai , Michio Sasaoka , Mitsuo Akada , Yoshio Yabuhara , Kouji Mori , Akihiro Murakami , Hiroshi Hama
IPC分类号： A01N33/12 , A01N35/02 , A01N37/20 , C07C211/63 , C07C217/08 , C07C225/06 , C07C233/38 , C08F12/26 , C08F16/28 , C08F16/36 , C08F20/60 , C08F26/02 , D06M15/21 , D06M15/267 , D06M15/285 , D06M15/347 , D06M16/00 , C08F220/56 , C08F216/36 , C08F226/02
CPC分类号： D06M15/347 , A01N33/12 , A01N35/02 , A01N37/20 , C07C211/63 , C07C217/08 , C07C225/06 , C07C233/38 , C08F12/26 , C08F16/28 , C08F16/36 , C08F20/60 , C08F26/02 , D06M15/21 , D06M15/267 , D06M15/285 , D06M16/00 , D06M2101/06
摘要： The present invention provides a polymerizable monomer represented by the general formula (1), polymer thereof, process for preparing the polymer and the use of the polymer ##STR1## wherein R.sup.1 is a hydrogen atom, methyl, chlorine or cyano, R.sup.2 is alkylene having 1 to 4 carbon atoms, R.sup.3 is alkylene having 3 to 10 carbon atoms, R.sup.4 is alkyl having 6 to 18 carbon atoms, R.sup.6 to R.sup.8 are the same or different and are each alkyl or substituted alkyl having 1 to 3 carbon atoms, A is methylene, phenylene, substituted phenylene, --CH.sub.2 O--, --CH.sub.2 CH.sub.2 O--, --CO--, --CO--N(R.sup.9)-- (wherein R.sup.9 is a hydrogen atom or methyl) or vinylene, m is 0 or 1, and X and Y are the same or different, and are each a monovalent anion or form a bivalent anion when taken together.
摘要翻译： PCT No.PCT / JP93 / 00924 Sec。 371日期：1994年7月11日 102（e）日期1994年7月11日PCT提交1993年7月6日PCT公布。公开号WO94 / 01474 日本1990年1月20日。本发明提供由通式（1）表示的可聚合单体，其聚合物，聚合物的制备方法和聚合物的用途。（1）其中R1是氢原子，甲基，氯或氰基，R 2是具有1至4个碳原子的亚烷基，R 3是具有3至10个碳原子的亚烷基，R 4是具有6至18个碳原子的烷基，R 6至R 8相同或不同，并且各自是烷基或取代的 A为亚甲基，亚苯基，取代亚苯基，-CH 2 O - ， - CH 2 CH 2 O-，-CO - ， - CO-N（R 9） - （其中R 9为氢原子或甲基）或亚乙烯基， m为0或1，X和Y相同或不同，并且各自为一价阴离子或一起形成二价阴离子。

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