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21. 发明授权

US08372286B2 Method for production of separation media 失效
标题翻译：分离介质的生产方法
公开(公告)号：US08372286B2
公开(公告)日：2013-02-12
申请号：US12863448
申请日：2009-01-30
申请人： Gunnar Glad , Bo-Lennart Johansson , Jean-Luc Maloisel , Nils Norrman , Ake Stenholm
发明人： Gunnar Glad , Bo-Lennart Johansson , Jean-Luc Maloisel , Nils Norrman , Ake Stenholm
IPC分类号： B01D15/08
CPC分类号： B01J20/291 , B01J20/262 , B01J20/2809 , B01J20/285 , B01J20/286 , B01J20/287 , B01J20/288 , B01J20/3212 , B01J20/3268 , B01J20/3274 , B01J20/3293 , B01J39/26 , B01J41/20 , B01J2220/52
摘要： The present invention relates to a method for production of separation media using a so called Spinning Disc technology wherein the porosities of the beads are optimized in such a way that a desired biomolecule may be separated from a complex sample. The method comprises the following steps: a) feeding a 4-8% polysaccharide solution, which has a viscosity within 350-450 mPas, at 65-75° C. to one or more spinning discs at 3001-3010 rpm to form polysaccharide beads; b) capturing said formed polysaccharide beads in a capturing bath; wherein the porosity of the polysaccharide beads is controlled by varying the temperature of the capturing between 15 and 27° C., preferably between 17.5 and 24.6° C. The method yields porosities that prevent molecules larger than 150 000 g/mol to diffuse into the beads. The invention also relates to separation media produced by the method and use thereof for purification of biomolecules, in particular monoclonal antibodies.
摘要翻译：本发明涉及使用所谓的旋转圆盘技术生产分离介质的方法，其中珠子的孔隙度以使得所需生物分子可以与复杂样品分离的方式进行优化。该方法包括以下步骤：a）将具有350-450mPa·s粘度的4-8％多糖溶液在65-75℃下以3001-3010rpm向一个或多个纺丝盘送入，以形成多糖珠粒 ; b）在捕获浴中捕获所述形成的多糖珠粒; 其中多糖珠的孔隙率通过在15和27℃之间，优选在17.5和24.6℃之间改变捕获温度来控制。该方法产生阻止大于150000g / mol的分子扩散到珠子本发明还涉及通过其用于纯化生物分子，特别是单克隆抗体的方法和用途产生的分离培养基。

22. 发明授权

US08114280B2 Separation matrix and method of purification 有权
标题翻译：分离基质和纯化方法
公开(公告)号：US08114280B2
公开(公告)日：2012-02-14
申请号：US11570530
申请日：2005-06-22
申请人： Jean-Luc Maloisel , Bo-Lennart Johansson , Gunnar Glad , Nils Norrman
发明人： Jean-Luc Maloisel , Bo-Lennart Johansson , Gunnar Glad , Nils Norrman
IPC分类号： B01D15/08
CPC分类号： B01J20/3212 , B01D15/426 , B01J20/286 , B01J20/289 , B01J20/3219 , B01J20/3251
摘要： The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilized, wherein said ligands comprise at least one sulphonamide and the R group of the sulphonyl comprises an aromatic group. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to the separation matrix.
摘要翻译：本发明涉及由已经固定有配体的多孔载体组成的分离基质，其中所述配体包含至少一个磺酰胺，并且磺酰基的R基包括芳族基团。磺酰胺的氮可以是仲胺或叔胺。本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到分离基质上分离免疫球蛋白样化合物的方法。

23. 发明申请

US20100298548A1 METHOD FOR PRODUCTION OF SEPARATION MEDIA 失效
标题翻译：分离介质的生产方法
公开(公告)号：US20100298548A1
公开(公告)日：2010-11-25
申请号：US12863448
申请日：2009-01-30
申请人： Gunnar Glad , Bo-Lennart Johansson , Jean-Luc Maloisel , Nils Norrman , Ake Stenholm
发明人： Gunnar Glad , Bo-Lennart Johansson , Jean-Luc Maloisel , Nils Norrman , Ake Stenholm
IPC分类号： C07K16/00 , B29B9/10 , B01D15/04 , B29B9/12
CPC分类号： B01J20/291 , B01J20/262 , B01J20/2809 , B01J20/285 , B01J20/286 , B01J20/287 , B01J20/288 , B01J20/3212 , B01J20/3268 , B01J20/3274 , B01J20/3293 , B01J39/26 , B01J41/20 , B01J2220/52
摘要： The present invention relates to a method for production of separation media using a so called Spinning Disc technology wherein the porosities of the beads are optimized in such a way that a desired biomolecule may be separated from a complex sample. The method comprises the following steps: a) feeding a 4-8% polysaccharide solution, which has a viscosity within 350-450 mPas, at 65-75° C. to one or more spinning discs at 3001-3010 rpm to form polysaccharide beads; b) capturing said formed polysaccharide beads in a capturing bath; wherein the porosity of the polysaccharide beads is controlled by varying the temperature of the capturing between 15 and 27° C., preferably between 17.5 and 24.6° C. The method yields porosities that prevent molecules larger than 150 000 g/mol to diffuse into the beads. The invention also relates to separation media produced by the method and use thereof for purification of biomolecules, in particular monoclonal antibodies.
摘要翻译：本发明涉及使用所谓的旋转圆盘技术生产分离介质的方法，其中珠子的孔隙度以使得所需生物分子可以与复杂样品分离的方式进行优化。该方法包括以下步骤：a）将具有350-450mPa·s粘度的4-8％多糖溶液在65-75℃下以3001-3010rpm向一个或多个纺丝盘送入，以形成多糖珠 ; b）在捕获浴中捕获所述形成的多糖珠粒; 其中多糖珠的孔隙率通过在15和27℃之间，优选在17.5和24.6℃之间改变捕获温度来控制。该方法产生阻止大于150000g / mol的分子扩散到珠子本发明还涉及通过其用于纯化生物分子，特别是单克隆抗体的方法和用途产生的分离培养基。

24. 发明申请

US20100282610A1 METHOD FOR SAMPLE APPLICATION 失效
标题翻译：样品申请方法
公开(公告)号：US20100282610A1
公开(公告)日：2010-11-11
申请号：US12812466
申请日：2009-01-19
申请人： Gunnar Glad , Nils Norrman , Susanna Lindman
发明人： Gunnar Glad , Nils Norrman , Susanna Lindman
IPC分类号： B01D57/02 , C07K1/14
CPC分类号： G01N27/44743 , B01L3/5082 , B01L2400/0421 , B01L2400/043 , G01N1/405 , G01N2446/00
摘要： The present invention relates to a method for sample application and separation. More closely, the invention relates to convenient direct loading of a biomolecule sample via magnetic beads to, for example, a gel before electrophoresis. In this way, the invention combines elution and application steps with minimal losses of sample. Thus, the invention relates to a method for sample application of biomolecules on a separation media, comprising the following steps: a) obtaining said biomolecules from a sample by magnetic beads; b) applying the magnetic beads with the biomolecules to a separation medium; c) releasing the biomolecules into the separation media, and d) separation of the biomolecules from each other in the separation medium.
摘要翻译：本发明涉及一种样品应用和分离方法。更为详细地，本发明涉及通过磁珠将生物分子样品直接加载到例如电泳前的凝胶。以这种方式，本发明将洗脱和应用步骤组合在一起，同时具有最小的样品损失。因此，本发明涉及在分离介质上样品应用生物分子的方法，包括以下步骤：a）通过磁珠从样品中获得所述生物分子; b）将具有生物分子的磁珠施加到分离介质; c）将生物分子释放到分离介质中，以及d）在分离介质中分离生物分子。

25. 发明申请

US20100016564A1 METHOD OF PREPARING AN IMMOBILISED METAL ION CHROMATOGRAPHY ADSORBENT AND METHODS OF PURIFYING PROTEINS, PEPTIDES OR POLYNUCLEOTIDES 审中-公开
标题翻译：制备固定化金属离子色谱吸附剂的方法和净化蛋白质，肽或多核苷酸的方法
公开(公告)号：US20100016564A1
公开(公告)日：2010-01-21
申请号：US12297241
申请日：2007-05-28
申请人： Lars C. Andersson , Makonnen Belew , Tesfai Gebru , Gunnar Glad , Helena Lindgren , Nils Norrman
发明人： Lars C. Andersson , Makonnen Belew , Tesfai Gebru , Gunnar Glad , Helena Lindgren , Nils Norrman
IPC分类号： C07K1/22 , C07H23/00
CPC分类号： B01J20/3265 , B01D15/3828 , B01J20/289 , B01J45/00
摘要： The present invention relates to a method of preparing an immobilised metal ion affinity chromatography (IMAC) adsorbent, which comprises to provide chromatography ligands comprised of alkylene diamine triacetic acid, or a derivative thereof, and coupling thereof to a carrier via nitrogen. In an advantageous embodiment, the alkylene diamine triacetic acid is ethylene diaminetriacetic acid (ED3A).
摘要翻译：本发明涉及一种制备固定金属离子亲和层析（IMAC）吸附剂的方法，其包括提供由亚烷基二胺三乙酸或其衍生物组成的色谱配体，以及通过氮与载体的偶联。在有利的实施方案中，亚烷基二胺三乙酸是乙二胺三乙酸（ED3A）。

26. 外观设计

USD595423S1 Magnetic rack 有权
标题翻译：磁架
公开(公告)号：USD595423S1
公开(公告)日：2009-06-30
申请号：US29329147
申请日：2008-12-10
申请人： Bjorn A. Johansson , Nils Norrman , Henrik Ostlin , Sven Wounder
设计人： Bjorn A. Johansson , Nils Norrman , Henrik Ostlin , Sven Wounder

27. 发明申请

US20090092837A1 MAGNETIC BEADS 审中-公开
标题翻译：磁珠
公开(公告)号：US20090092837A1
公开(公告)日：2009-04-09
申请号：US12282650
申请日：2007-03-20
申请人： Andreas Axen , Eva Holmgren , Nils Norrman , Tobias E. Soderman
发明人： Andreas Axen , Eva Holmgren , Nils Norrman , Tobias E. Soderman
IPC分类号： B32B5/16 , B05D7/00
CPC分类号： G01N33/5434 , B22F2998/00 , B82Y25/00 , C12N11/08 , C12N11/10 , H01F1/0054 , H01F1/37 , Y10T428/2998 , B22F1/0062
摘要： The present invention relates to magnetic beads in the form of composite beads with an inner core of metal particles, which are coated with an inert synthetic polymer and thereafter a hydrophilic porous polymer, preferably dextran. This provides porous biocompatible beads without metal leakage. This construction also allows for simple and convenient handling of cell expansion media by magnetism.
摘要翻译：本发明涉及具有金属颗粒内核的复合珠形式的磁珠，其中涂覆有惰性合成聚合物，然后涂覆亲水性多孔聚合物，优选葡聚糖。这提供了多孔的生物相容珠，而没有金属泄漏。这种结构还允许通过磁性简单和方便地处理细胞膨胀介质。

28. 发明申请

US20080283792A1 Separation Medium with Various Functionalities 审中-公开
标题翻译：具有各种功能的分离介质
公开(公告)号：US20080283792A1
公开(公告)日：2008-11-20
申请号：US12090403
申请日：2006-10-23
申请人： Andreas Axen , Eva Holmgren , Nils Norrman , Tobias Soderman
发明人： Andreas Axen , Eva Holmgren , Nils Norrman , Tobias Soderman
IPC分类号： B03C1/01
CPC分类号： G01N33/5434 , B01J20/0229 , B01J20/06 , B01J20/24 , B01J20/26 , B01J20/28009 , B01J20/28026 , B01J20/286 , B01J20/3028 , B01J20/3244 , B01J20/3268 , B01J20/327 , B01J20/3272 , B01J20/3274 , B01J20/3285 , B01J20/3289 , B01J20/3293 , B03C1/01 , G01N33/54326 , H01F1/0054 , H01F1/36
摘要： The present invention relates to a separation medium with various functionalities suitable for, for example, isolation of proteins, cells, and viruses and also for diagnostic applications and cell cultivation. The separation medium comprises magnetic metal particles, preferably coated with an inert synthetic polymer, and pre-functionalised beads. These particles and beads are provided encapsulated in a hydrophilic porous polymer, preferably agarose. The beads may be used for cell cultivation or for chromatography. When the beads are used for chromatography the agarose layer may be provided with ligands having affinity for selected biomolecules.
摘要翻译：本发明涉及具有适合于例如蛋白质，细胞和病毒分离以及诊断应用和细胞培养的各种功能的分离介质。分离介质包括优选涂覆有惰性合成聚合物的磁性金属颗粒和预功能化珠。提供这些颗粒和珠粒包封在亲水性多孔聚合物中，优选琼脂糖。珠粒可用于细胞培养或色谱法。当珠子用于色谱法时，琼脂糖层可以具有对选定的生物分子具有亲和力的配体。

29. 发明授权

US08413528B2 Device and method for separation of proteins and other biomolecules 有权
标题翻译：用于分离蛋白质和其他生物分子的装置和方法
公开(公告)号：US08413528B2
公开(公告)日：2013-04-09
申请号：US12597968
申请日：2008-05-20
申请人： Nils Norrman , Ronnie Palmgren
发明人： Nils Norrman , Ronnie Palmgren
IPC分类号： G01N1/22 , B01L3/02
CPC分类号： G01N30/84 , B01L3/0275 , B01L3/508 , B01L2200/026 , B01L2300/0681 , B01L2400/0605 , G01N30/6065 , G01N2030/009 , G01N2030/062
摘要： The present invention relates to a device and method for separation of proteins and other biomolecules. A preferred use is for sample preparation of crude as well as pre-fractionated samples. In a preferred embodiment, the device of the present invention is a pipette tip having dual channels, one for inlet of sample and one for outlet. The outlet, but not the inlet, channel is provided with sample separation media for separation of a desired biomolecule from a sample. The flow through the sample separation media is unidirectional.
摘要翻译：本发明涉及分离蛋白质和其他生物分子的装置和方法。优选的用途是用于样品制备粗品以及预先分馏的样品。在优选实施例中，本发明的装置是具有双通道的移液管尖端，一个用于样品入口，一个用于出口。出口但不是入口通道设置有样品分离介质，用于将期望的生物分子与样品分离。通过样品分离介质的流动是单向的。

30. 发明申请

US20110155668A1 SEPARATION MEDIUM FOR CHROMATOGRAPHY OF VARIOUS BIOMOLECULES 审中-公开
标题翻译：分离介质用于各种生物分子的色谱分析
公开(公告)号：US20110155668A1
公开(公告)日：2011-06-30
申请号：US13002878
申请日：2009-06-04
申请人： Gunnar Glad , Bo-Lennart Johansson , Jean-Luc Maloisel , Nils Norrman
发明人： Gunnar Glad , Bo-Lennart Johansson , Jean-Luc Maloisel , Nils Norrman
IPC分类号： B01D15/36 , B01J20/22 , B01J20/26 , B01J20/24
CPC分类号： B01D15/3828 , B01D15/166 , B01D15/36 , B01J20/28092 , B01J20/285 , B01J20/286 , B01J20/3242 , B01J20/3293 , C07K1/16 , C07K1/22 , C07K16/00
摘要： The present invention relates to a separation medium, comprising an inner core of a porous material provided with charged ligands, and an outer lid comprising a porous material provided with charged ligands, wherein the charge of the ligands in the inner core is opposite that of the charge of the ligands in the lid. The present invention also relates to a method for biomolecule separation comprising applying a sample to the above separation medium, wherein large molecules are prevented from entering the medium by charge repulsion from the medium and small molecules are captured in the inner core.
摘要翻译：本发明涉及一种分离介质，其包括具有带电配位体的多孔材料的内核和包含带有带电配位体的多孔材料的外盖，其中内芯中配体的电荷与盖中配体的电荷。本发明还涉及一种用于生物分子分离的方法，包括将样品施加到上述分离介质中，其中通过从介质的电荷排斥来防止大分子进入介质，并且小分子被捕获在内核中。

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