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    • 23. 发明申请
    • Microscopic Image Capturing Device
    • 显微图像捕获装置
    • US20090002811A1
    • 2009-01-01
    • US11886255
    • 2006-03-17
    • Shigeru UchiyamaTakayuki InoueMasatoshi Okugawa
    • Shigeru UchiyamaTakayuki InoueMasatoshi Okugawa
    • G02B21/26G02B21/36
    • G02B21/241G02B21/26G02B21/365
    • The present invention relates to a microscopic image capturing apparatus having a structure that, in scanning an imageable area of an imaging unit in a predetermined direction in an imaging object area, in which a sample is present, can reliably set a focal point of the imaging unit on each imaging position set inside the imaging object area regardless of the type of focusing actuator. The microscopic image capturing apparatus has a sample setting stage having a sample setting surface that is inclined with respect to a scan plane orthogonal to an optical axis of an objective lens. By moving the sample setting stage, which has such a sample setting surface, along the scan plane in a manner such that the distance in the optical axis direction between the imaging unit and the sample setting surface varies monotonously, the focal point position of the imaging unit is adjusted in only one direction along the optical axis of the objective lens. In this case, even when a stepping motor is applied as the focusing actuator, the occurrence of lost motion in a driving system based on the stepping motor is prevented reliably and the focal point of the imaging unit can be set reliably on each imaging position set inside the imaging object area.
    • 本发明涉及一种显微图像拍摄装置,其特征在于,在成像对象区域内,在成像对象区域内,在成像单元的预定方向的可成像区域中进行扫描时,能够可靠地设定成像的焦点 在成像对象区域内设置的每个成像位置上的单位,而与聚焦致动器的类型无关。 微观图像拍摄装置具有样品设定台,该样品设定台具有相对于与物镜的光轴正交的扫描面倾斜的样品设定面。 通过沿着扫描平面移动具有这样的样品设定面的样品设定台,使得成像单元和样品设定面之间的光轴方向的距离单调变化,成像的焦点位置 单元沿着物镜的光轴在一个方向上被调整。 在这种情况下,即使施加步进电机作为聚焦致动器,也可以可靠地防止基于步进电动机的驱动系统中的运动的发生,并且能够可靠地将摄像部的焦点设定在各摄像位置组 在成像对象区域内。
    • 24. 发明申请
    • Autofocus Device and Microscope Using the Same
    • 自动对焦设备和显微镜使用相同
    • US20080283722A1
    • 2008-11-20
    • US11658499
    • 2005-07-20
    • Shigeru UchiyamaTakayuki InoueMasatoshi Okugawa
    • Shigeru UchiyamaTakayuki InoueMasatoshi Okugawa
    • G02B27/64G02B21/00
    • G02B21/367G02B7/38H04N5/23212
    • A microscope apparatus 1A is formed with a CCD camera 30 that acquires an image of a sample S, a light guiding optical system 20 that guides an optical image of the sample S to the camera 30, an optical system driving section 25 that drives the camera 30 and the optical system 20 to change a focal position on the sample S in a z-axis direction, and a control device 50 that includes a focal point control section 51. The focal point control section 51 calculates a first focal point measurement value from a plurality of images acquired by a first focal point measurement that is executed while continuously changing the focal position in one direction, calculates a second focal point measurement value from a plurality of images acquired by a second focal point measurement that is executed while continuously changing the focal position in a direction opposite that of the first focal point measurement, and determines an in-focus position for the sample S based on the first and second focal point measurement values. This realizes an automatic focusing device that is capable of accurately determining an in-focus position in a short time and a microscope apparatus using the same.
    • 显微镜装置1A形成有获取样本S的图像的CCD照相机30,将样本S的光学图像引导到照相机30的导光光学系统20,驱动照相机30的光学系统驱动部25 照相机30和光学系统20,以在z轴方向上改变样本S上的焦点位置,以及包括焦点控制部分51的控制装置50.焦点控制部分51计算第一焦点测量值 从通过在一个方向连续地改变焦点位置而执行的第一焦点测量获取的多个图像中,从通过在连续变化时执行的第二焦点测量获得的多个图像计算第二焦点测量值 在与第一焦点测量相反的方向上的焦点位置,并且基于第一和第二焦点p确定样本S的对焦位置 软膏测量值。 这实现了能够在短时间内精确地确定对焦位置的自动聚焦装置和使用其的显微镜装置。
    • 29. 发明申请
    • Image acquiring apparatus, image acquiring method, and image acquiring program
    • 图像获取装置,图像获取方法和图像获取程序
    • US20070206096A1
    • 2007-09-06
    • US11477802
    • 2006-06-30
    • Jeremy CookeTakayuki Inoue
    • Jeremy CookeTakayuki Inoue
    • H04N5/225
    • G02B21/367
    • A semi-automatic mode according to which an operator makes a necessary confirmation when an image pickup condition for a micro image is set with reference to a macro image is provided in controlling the acquisition of the macro image, the setting of the image pickup condition, and the acquisition of the micro image with respect to each of a plurality of samples S stored in a sample storage unit 11, and in the semi-automatic mode, the sample S whose macro image has been acquired by a macro image acquiring unit 20 is placed at a standby position without being moved directly to an image acquiring position for a micro image acquiring unit 30. According to this structure, image acquisition with respect to each of the samples S can be efficiently performed, and an operational burden imposed on the operator is reduced. Accordingly, it becomes possible to realize an image acquiring apparatus, an image acquiring method, and an image acquiring program each of which is capable of performing image acquisition with respect to each of the samples with high efficiency.
    • 提供一种半自动模式,当在宏观图像上设置用于微图像的图像拾取条件时,操作者进行必要的确认,以控制宏图像的获取,图像拾取条件的设置, 并且相对于存储在采样存储单元11中的多个采样S中的每一个采集微图像,并且在半自动模式中,宏图像获取单元20获取的宏图像的采样S是 放置在待机位置而不被直接移动到用于微图像获取单元30的图像获取位置。 根据该结构,能够有效地进行与各样本S相关的图像采集,降低了对操作者的操作负担。 因此,可以实现能够以高效率对每个样本进行图像获取的图像获取装置,图像获取方法和图像获取程序。
    • 30. 发明申请
    • Fluorescent correalated spectrometric analysis device
    • 荧光相关光谱分析装置
    • US20060262301A1
    • 2006-11-23
    • US10545393
    • 2004-02-13
    • Hirohiko WatanabeMotoyuki WatanabeTakayuki Inoue
    • Hirohiko WatanabeMotoyuki WatanabeTakayuki Inoue
    • G01J3/30G01N21/64
    • G01N21/6456G01J3/2803G01J3/2823G01J3/2889G01J3/4406G01J2003/2813G01J2003/282G01N21/645G01N2021/6417G01N2021/6423H04N5/3454H04N5/372
    • A fluorescence correlation spectroscopy analyzer 1 is equipped with an excitation light illuminating optical system 21, a fluorescence imaging optical system 22, a CCD camera 15, and a data analyzer 16. The excitation light illuminating optical system 21 illuminates excitation light onto a predetermined region of a measured sample S. The fluorescence imaging optical system 22 images the fluorescence generated at the measured sample S onto the photodetection surface of the CCD camera 15. The CCD camera 15 performs photoelectric conversion of the fluorescence made incident onto the photodetection surface in accordance with the respective pixels and outputs the charges generated by the photoelectric conversion as detection signals from an output terminal. The data analyzer 16 inputs the detection signals based on the charges generated at the pixels, among the pixels of the CCD camera 15, that belong to an analyzed pixel set and computes autocorrelation functions of the input detection signals according to each pixel. A fluorescence correlation spectroscopy analyzer, which is enabled to perform fluorescence correlation spectroscopy analysis on multiple points of a measured sample simultaneously and at high speed, is thus provided.
    • 荧光相关光谱分析仪1配备有激发光照明光学系统21,荧光成像光学系统22,CCD照相机15和数据分析器16。 激发光照射光学系统21将激发光照射到测量样品S的预定区域上。荧光成像光学系统22将在测量样品S处产生的荧光图像到CCD照相机15的光电检测表面上。 CCD摄像机15根据各像素对入射到光检测面上的荧光进行光电转换,并将从光电转换产生的电荷作为检测信号从输出端子输出。 数据分析器16基于在CCD摄像机15的像素中产生的属于分析像素组的像素产生的电荷输入检测信号,并根据每个像素计算输入检测信号的自相关函数。 因此,提供了可以同时进行高速度地对多个点进行荧光相关光谱分析的荧光相关光谱分析仪。