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    • 13. 发明授权
    • Biofilter or compost support assembly
    • 生物过滤器或堆肥支撑组件
    • US07422895B1
    • 2008-09-09
    • US10828875
    • 2004-04-21
    • Jeffrey Hallsten
    • Jeffrey Hallsten
    • C12M1/16F26B25/10
    • B01D53/85F26B9/103Y02A50/2359
    • A modular support system for a bed of organic filter medium has provision for adjusting the distribution of gases in the plenum defined below the filter bed platform. Once the filter bed platform has been fully erected, with a gas inlet below the platform, slide gates are positioned in special modules which define a gas distribution channel, preferably extending in a series to form a line in the assembled support platform. The slide gates, one at each side of the special flow distribution modules, are adjusted as to the depth of penetration down into the plenum, so as to close the sides of the flow distribution channel to varying degrees along the length of the channel, effective to balance the flow of gases up through the perforations in the platform modules. The proper gate setting can be determined empirically after erection of the filter bed platform, by flowing gas through the platform and checking the distribution of gas exiting the holes in the modules. The gate settings can be adjusted until optimum balance of flow is achieved throughout the area of the platform.
    • 用于有机过滤介质床的模块化支撑系统提供了用于调节在过滤床平台下方定义的气室中的气体分布。 一旦过滤床平台已经完全竖立,在平台下方具有气体入口,滑动门被定位在限定气体分配通道的特殊模块中,优选地以串联方式延伸以在组装的支撑平台中形成线。 特殊流量分配模块的每一侧的一个滑动门被调整为穿透进入通风室的深度,以便沿着通道的长度将流量分配通道的侧面不同程度地封闭,有效 以平衡通过平台模块中的穿孔的气体流量。 在安装过滤床平台之后,通过使气体流过平台并检查离开模块中的孔的气体分布,可以凭经验确定适当的浇口设置。 可以调整门设置,直到平台的整个区域达到最佳流量平衡。
    • 16. 发明授权
    • Method and device for detecting bacteriophage using contrast-coloring
and precipitable dyes
    • 使用对比色和可沉淀染料检测噬菌体的方法和装置
    • US6090541A
    • 2000-07-18
    • US350978
    • 1999-07-09
    • James H. WicksGary E. KrejcarekMichael G. Williams
    • James H. WicksGary E. KrejcarekMichael G. Williams
    • C12M1/16C12Q1/04C12Q1/70C12M1/34
    • C12Q1/04C12Q2334/52
    • The use of a precipitable dye and a contrast-coloring dye together enhance visualization of plaques in confluent lawns of bacteria in bacteriophage and bacteria assays. A test sample suspected of containing a bacteriophage is combined with bacteria capable of replicating the bacteriophage, and applied to a water-proof surface to form a support for bacterial growth. The support is provided with the contrast-coloring dye and precipitable dye, and nutrients and salts capable of supporting growth of the bacteria. A lawn of bacteria is formed on the support, and plaques detected on the lawn indicate presence of the bacteriophage. The plaques contain a precipitate formed by enzymatic cleavage of the precipitable dye by an enzyme of the bacterial lawn. A similar procedure is used for detecting bacteria, except that a test sample suspected of containing a bacteria is combined with bacteriophage capable of replicating in the bacteria, and plaques detected indicate presence of the bacteria. The bacteriophage and bacteria assays are carried out with a disposable device containing at least one well having a water-proof surface and a depth of about at least 5 millimeters. A hydratable material containing the contrast-coloring dye and precipitable dye is positioned on the surface. The well may contain substantially vertical sides with a removable cover resting on top of the sides.
    • 使用可沉淀的染料和对比色染料一起增强细菌在细菌和细菌测定中的汇合草坪中斑块的可视化。 怀疑含有噬菌体的试样与能够复制噬菌体的细菌结合,并施用于防水表面以形成细菌生长的载体。 该支撑体具有对比色染料和可沉淀染料,以及能够支持细菌生长的营养物和盐。 在支撑体上形成细菌的草坪,在草坪上检测到的斑块表示存在噬菌体。 斑块含有通过细菌草坪的酶酶切割可沉淀染料形成的沉淀物。 除了将疑似含有细菌的检测样品与能够在细菌中复制的噬菌体组合以外,使用类似的方法检测细菌,并且检测到的斑块表明存在细菌。 噬菌体和细菌测定用含有至少一个具有防水表面和约至少5毫米深度的孔的一次性装置进行。 含有对比色染料和可沉淀染料的可水合材料位于表面上。 井可以包含基本上垂直的侧面,其中可移除的盖搁置在侧面的顶部。
    • 18. 发明授权
    • Method for detecting bacteria using bacteriophage, contrast-coloring dye
and precipitable dye
    • 使用噬菌体,对比色染料和可沉淀染料检测细菌的方法
    • US5958675A
    • 1999-09-28
    • US844145
    • 1997-04-18
    • James H. WicksGary E. KrejcarekMichael G. Williams
    • James H. WicksGary E. KrejcarekMichael G. Williams
    • C12M1/16C12Q1/04C12Q1/70C12M1/34
    • C12Q1/04C12Q2334/52
    • Bacteria are detected in a test sample by contacting the test sample with a bacteriophage that is capable of replicating in the bacteria, adding the resultant sample to a water-proof surface of a support for bacterial growth that contains a contrast-coloring dye and a precipitable dye, forming a bacterial lawn of a bacteria in which the bacteriophage can replicate on the support and detecting plaques on the bacterial lawn as an indication of the presence of the bacteria. The combination of precipitable dye and contrast-coloring dye improves visualization of plaques. A precipitate is formed in plaques by enzymatic cleavage of the precipitable dye by an enzyme of the bacterial lawn. A procedure for detecting bacteriophage is similar to that for detecting bacteria, except that a test sample suspected of containing bacteriophage is combined with bacteria in which the bacteriophage can replicate, and plaques detected indicate presence of the bacteriophage. The bacteria and bacteriophage detections are carried out with a disposable device containing at least one well having a water-proof surface and substantially vertical sides that extend at least 5 millimeters in height from the surface. A hydratable material containing the precipitable dye and the contrast-coloring dye is positioned on the water-proof surface. A removable cover rests on top of the sides of the well.
    • 通过使测试样品与能够在细菌中复制的噬菌体接触,将测试样品中的细菌检测到细菌,将所得样品加入含有对比色染料和可沉淀的染色染料的细菌生长支持体的防水表面 染色,形成细菌的细菌草坪,其中噬菌体可以在载体上复制并检测细菌草坪上的斑块,作为细菌存在的指示。 可沉淀的染料和对比色染料的组合改善斑块的可视化。 通过细菌草坪的酶酶切割可沉淀的染料,在噬菌斑中形成沉淀物。 用于检测噬菌体的方法与用于检测细菌的方法类似,除了将疑似含有噬菌体的试验样品与噬菌体可以复制的细菌结合,并且检测到斑块表明存在噬菌体。 细菌和噬菌体检测用一次性装置进行,该装置包含至少一个具有防水表面的孔,并且基本垂直的侧面从该表面延伸出至少5毫米的高度。 含有可沉淀染料和对比色染料的可水合材料位于防水表面上。 可拆卸的盖子位于井的两侧的顶部。
    • 20. 发明授权
    • Apparatus for culturing microorganism
    • 微生物培养装置
    • US5494823A
    • 1996-02-27
    • US284506
    • 1994-08-08
    • Masahiro TakemotoHiroshige Yamauchi
    • Masahiro TakemotoHiroshige Yamauchi
    • C12M1/22C12M1/16C12M1/34C12M3/04
    • C12M23/04C12M23/22C12M23/38C12M25/06C12M33/02
    • The object of the present invention is to permeate sample solution uniformly into an absorbent fibrous sheet. The gelation agent soluble in cold water absorbs moisture and forms a gel, thereby forming a gel medium. Microorganisms are thus cultivated. The surface of a waterproof plate 2 is coated with the mixture 3 of the gelation agent soluble in cold water and microorganism culture medium. Further, an absorbent fibrous sheet 4 is laid on the mixture 3. When the sample solution including microorganisms is poured onto the absorbent sheet 4, the solution is dispersed uniformly over the entire of the absorbent fibrous sheet 4 by the phenomenon of capillary attraction resulting from overlapped fibers. Subsequently, the mixture 3 of the gelation agent and culture medium coated on the plate 2 absorbs moisture and forms a gel. The fibers of the absorbent fibrous sheet 4 are buried and adhered into the mixture 3, thereby forming a gel medium for the culture of microorganisms.
    • PCT No.PCT / JP93 / 01440 Sec。 371日期1994年8月8日 102(e)日期1994年8月8日PCT提交1993年10月4日PCT公布。 公开号WO94 / 09151 日本1994年4月28日。本发明的目的是将样品溶液均匀地渗透到吸收性纤维片材中。 可溶于冷水的凝胶剂吸收水分并形成凝胶,从而形成凝胶介质。 因此培育微生物。 防水板2的表面涂覆有可溶于冷水和微生物培养基的凝胶剂的混合物3。 此外,将吸收性纤维片材4放置在混合物3上。当将包含微生物的样品溶液倒在吸收性片材4上时,通过毛细吸引力的现象将溶液均匀地分散在吸收性纤维片材4的全部上 重叠的纤维。 随后,凝胶化剂和涂覆在板2上的培养基的混合物3吸收水分并形成凝胶。 将吸收性纤维片4的纤维埋入并粘附到混合物3中,由此形成用于培养微生物的凝胶介质。