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1. 发明授权

US07001719B2 Devices and methods for microorganism detection 有权
标题翻译：用于微生物检测的装置和方法
公开(公告)号：US07001719B2
公开(公告)日：2006-02-21
申请号：US10818092
申请日：2004-04-05
申请人： James H. Wicks , Carl A. Adams , Gary E. Krejcarek
发明人： James H. Wicks , Carl A. Adams , Gary E. Krejcarek
IPC分类号： C12Q1/70
CPC分类号： C12M41/36
摘要： A device that includes at least two chambers separated by an activatable seal wherein upon activation of the seal the two chambers are in communication, and further wherein at least one chamber of the device includes a biological assay reagent and methods of using same.
摘要翻译：一种装置，其包括由可激活密封件分开的至少两个腔室，其中在所述密封件激活时，所述两个腔室连通，并且其中所述装置的至少一个腔室包括生物测定试剂及其使用方法。

2. 发明授权

US06737266B1 Devices and methods for microorganism detection 失效
标题翻译：用于微生物检测的装置和方法
公开(公告)号：US06737266B1
公开(公告)日：2004-05-18
申请号：US09434586
申请日：1999-11-05
申请人： James H. Wicks , Carl A. Adams , Gary E. Krejcarek
发明人： James H. Wicks , Carl A. Adams , Gary E. Krejcarek
IPC分类号： C12M134
CPC分类号： C12M41/36
摘要： A device that includes at least two chambers separated by an activatable seal wherein upon activation of the seal the two chambers are in communication, and further wherein at least one chamber of the device includes a biological assay reagent and methods of using same.
摘要翻译：一种装置，其包括由可激活密封件分开的至少两个腔室，其中在所述密封件激活时，所述两个腔室连通，并且其中所述装置的至少一个腔室包括生物测定试剂及其使用方法。

3. 发明授权

US6090541A Method and device for detecting bacteriophage using contrast-coloring and precipitable dyes 有权
标题翻译：使用对比色和可沉淀染料检测噬菌体的方法和装置
公开(公告)号：US6090541A
公开(公告)日：2000-07-18
申请号：US350978
申请日：1999-07-09
申请人： James H. Wicks , Gary E. Krejcarek , Michael G. Williams
发明人： James H. Wicks , Gary E. Krejcarek , Michael G. Williams
IPC分类号： C12M1/16 , C12Q1/04 , C12Q1/70 , C12M1/34
CPC分类号： C12Q1/04 , C12Q2334/52
摘要： The use of a precipitable dye and a contrast-coloring dye together enhance visualization of plaques in confluent lawns of bacteria in bacteriophage and bacteria assays. A test sample suspected of containing a bacteriophage is combined with bacteria capable of replicating the bacteriophage, and applied to a water-proof surface to form a support for bacterial growth. The support is provided with the contrast-coloring dye and precipitable dye, and nutrients and salts capable of supporting growth of the bacteria. A lawn of bacteria is formed on the support, and plaques detected on the lawn indicate presence of the bacteriophage. The plaques contain a precipitate formed by enzymatic cleavage of the precipitable dye by an enzyme of the bacterial lawn. A similar procedure is used for detecting bacteria, except that a test sample suspected of containing a bacteria is combined with bacteriophage capable of replicating in the bacteria, and plaques detected indicate presence of the bacteria. The bacteriophage and bacteria assays are carried out with a disposable device containing at least one well having a water-proof surface and a depth of about at least 5 millimeters. A hydratable material containing the contrast-coloring dye and precipitable dye is positioned on the surface. The well may contain substantially vertical sides with a removable cover resting on top of the sides.
摘要翻译：使用可沉淀的染料和对比色染料一起增强细菌在细菌和细菌测定中的汇合草坪中斑块的可视化。怀疑含有噬菌体的试样与能够复制噬菌体的细菌结合，并施用于防水表面以形成细菌生长的载体。该支撑体具有对比色染料和可沉淀染料，以及能够支持细菌生长的营养物和盐。在支撑体上形成细菌的草坪，在草坪上检测到的斑块表示存在噬菌体。斑块含有通过细菌草坪的酶酶切割可沉淀染料形成的沉淀物。除了将疑似含有细菌的检测样品与能够在细菌中复制的噬菌体组合以外，使用类似的方法检测细菌，并且检测到的斑块表明存在细菌。噬菌体和细菌测定用含有至少一个具有防水表面和约至少5毫米深度的孔的一次性装置进行。含有对比色染料和可沉淀染料的可水合材料位于表面上。井可以包含基本上垂直的侧面，其中可移除的盖搁置在侧面的顶部。

4. 发明授权

US5958675A Method for detecting bacteria using bacteriophage, contrast-coloring dye and precipitable dye 失效
标题翻译：使用噬菌体，对比色染料和可沉淀染料检测细菌的方法
公开(公告)号：US5958675A
公开(公告)日：1999-09-28
申请号：US844145
申请日：1997-04-18
申请人： James H. Wicks , Gary E. Krejcarek , Michael G. Williams
发明人： James H. Wicks , Gary E. Krejcarek , Michael G. Williams
IPC分类号： C12M1/16 , C12Q1/04 , C12Q1/70 , C12M1/34
CPC分类号： C12Q1/04 , C12Q2334/52
摘要： Bacteria are detected in a test sample by contacting the test sample with a bacteriophage that is capable of replicating in the bacteria, adding the resultant sample to a water-proof surface of a support for bacterial growth that contains a contrast-coloring dye and a precipitable dye, forming a bacterial lawn of a bacteria in which the bacteriophage can replicate on the support and detecting plaques on the bacterial lawn as an indication of the presence of the bacteria. The combination of precipitable dye and contrast-coloring dye improves visualization of plaques. A precipitate is formed in plaques by enzymatic cleavage of the precipitable dye by an enzyme of the bacterial lawn. A procedure for detecting bacteriophage is similar to that for detecting bacteria, except that a test sample suspected of containing bacteriophage is combined with bacteria in which the bacteriophage can replicate, and plaques detected indicate presence of the bacteriophage. The bacteria and bacteriophage detections are carried out with a disposable device containing at least one well having a water-proof surface and substantially vertical sides that extend at least 5 millimeters in height from the surface. A hydratable material containing the precipitable dye and the contrast-coloring dye is positioned on the water-proof surface. A removable cover rests on top of the sides of the well.
摘要翻译：通过使测试样品与能够在细菌中复制的噬菌体接触，将测试样品中的细菌检测到细菌，将所得样品加入含有对比色染料和可沉淀的染色染料的细菌生长支持体的防水表面染色，形成细菌的细菌草坪，其中噬菌体可以在载体上复制并检测细菌草坪上的斑块，作为细菌存在的指示。可沉淀的染料和对比色染料的组合改善斑块的可视化。通过细菌草坪的酶酶切割可沉淀的染料，在噬菌斑中形成沉淀物。用于检测噬菌体的方法与用于检测细菌的方法类似，除了将疑似含有噬菌体的试验样品与噬菌体可以复制的细菌结合，并且检测到斑块表明存在噬菌体。细菌和噬菌体检测用一次性装置进行，该装置包含至少一个具有防水表面的孔，并且基本垂直的侧面从该表面延伸出至少5毫米的高度。含有可沉淀染料和对比色染料的可水合材料位于防水表面上。可拆卸的盖子位于井的两侧的顶部。

5. 发明授权

US4883641A Closure and container assembly for biological sterility indicator 失效
标题翻译：用于生物不育指标的闭合和容器装配
公开(公告)号：US4883641A
公开(公告)日：1989-11-28
申请号：US249982
申请日：1988-09-27
申请人： James H. Wicks , William E. Foltz
发明人： James H. Wicks , William E. Foltz
IPC分类号： A61L2/28 , B65D51/24 , C12Q1/22
CPC分类号： A61L2/28 , B65D51/241 , C12Q1/22 , Y10S435/81
摘要： A closure and container assembly biological sterility indicator having a container, a closure, test spores, growth and indicator media, and a frangible barrier for separating the growth medium from the test spores is shown. The closure is moveable in the container between open and closed positions. When in the open position, sterilant may flow into and out of the container via a tortuous pathway defined by the interior surface of the container or the exterior surface of the closure. The tortuous pathway may also include one or more openings in the wall of the closure. When the closure is in the closed position, the container is sealed. The sterility indicator may be assembled with the test spores impregnated on a spore strip which is placed in close proximity to the closure.
摘要翻译：示出了具有容器，封闭物，测试孢子，生长和指示剂介质以及用于将生长培养基与测试孢子分离的易碎屏障的封闭和容器组合生物不育指示器。封闭件可在容器中在打开和关闭位置之间移动。当处于打开位置时，消毒剂可以通过由容器的内表面或封闭件的外表面限定的曲折路径流入和流出容器。曲折路径还可以包括在封闭件的壁中的一个或多个开口。当封闭件处于关闭位置时，容器被密封。无菌指示剂可以与浸在放置在封闭物附近的孢子带上的测试孢子组装。

6. 发明授权

US06833251B2 Bacteria detection using immobilized enzyme substrates 有权
标题翻译：使用固定化酶底物的细菌检测
公开(公告)号：US06833251B2
公开(公告)日：2004-12-21
申请号：US10160594
申请日：2002-05-30
申请人： Carl A. Adams , Gary E. Krejcarek , James H. Wicks
发明人： Carl A. Adams , Gary E. Krejcarek , James H. Wicks
IPC分类号： C12Q104
CPC分类号： C12Q1/04 , C12Q1/34 , C12Q2334/20
摘要： Methods of detecting bacteria including the use of an immobilized enzyme substrate are provided.
摘要翻译：提供了检测细菌的方法，包括使用固定化酶底物。

7. 发明授权

US06436661B1 Bacteria and bacteriophage detection using immobilized enzyme substrates 有权
标题翻译：使用固定化酶底物的细菌和噬菌体检测
公开(公告)号：US06436661B1
公开(公告)日：2002-08-20
申请号：US09548157
申请日：2000-04-13
申请人： Carl A. Adams , Gary E. Krejcarek , James H. Wicks
发明人： Carl A. Adams , Gary E. Krejcarek , James H. Wicks
IPC分类号： C12Q104
CPC分类号： C12Q1/04 , C12Q1/34 , C12Q2334/20
摘要： Methods of detecting bacteria including the use of an immobilized enzyme substrate, and the immobilized enzyme substrate.
摘要翻译：检测细菌的方法，包括使用固定化酶底物和固定化酶底物。

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