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11. 发明授权

US6013119A Filtering system and method for incubators 失效
标题翻译：孵化器的过滤系统和方法
公开(公告)号：US6013119A
公开(公告)日：2000-01-11
申请号：US855899
申请日：1997-05-15
申请人： Michael D. Cecchi , Jacques Cohen , Brian Dale
发明人： Michael D. Cecchi , Jacques Cohen , Brian Dale
IPC分类号： B01D46/24 , B01D50/00 , B01D53/04 , B01L1/04 , C12M1/00 , B01D29/50 , C12M1/12
CPC分类号： B01D46/521 , B01D46/0024 , B01D46/2411 , B01D50/004 , B01D53/0415 , C12M41/14 , C12M41/34 , B01D2253/102 , B01D2257/708 , B01D2257/80 , B01D2267/40 , B01D53/261 , B01L1/04 , Y10S435/809
摘要： An improved filter system and method for filtering the gaseous environment of an incubator in which volatile organic compounds and particulate material are filtered from the gaseous environment. A filter is provided which includes a first filter medium to filter volatile organic compounds and a second filter medium to filter solid particulate material. A pump may be provided which draws a gas stream from the gaseous environment of the incubator and passes it through the filter. The pump and filter may be contained within a filter unit which may be used either internally or externally of the incubator.
摘要翻译：用于过滤挥发性有机化合物和颗粒物质从气态环境中过滤气候环境的改进的过滤系统和方法。提供了一种过滤器，其包括用于过滤挥发性有机化合物的第一过滤介质和用于过滤固体颗粒材料的第二过滤介质。可以提供泵，其从培养箱的气体环境抽取气体流并将其通过过滤器。泵和过滤器可以包含在可以在培养箱的内部或外部使用的过滤器单元中。

12. 外观设计

USD263518S Sole for footwear 失效
公开(公告)号：USD263518S
公开(公告)日：1982-03-30
申请号：US971544
申请日：1978-12-20
申请人： Jacques Cohen
设计人： Jacques Cohen

13. 外观设计

USD767164S1 Culture dish 有权
公开(公告)号：USD767164S1
公开(公告)日：2016-09-20
申请号：US29544437
申请日：2015-11-03
申请人： Timothy Schimmel , Jacques Cohen , Michael Cecchi
设计人： Timothy Schimmel , Jacques Cohen , Michael Cecchi

14. 外观设计

USD767160S1 Culture dish 有权
标题翻译：文化菜
公开(公告)号：USD767160S1
公开(公告)日：2016-09-20
申请号：US29544425
申请日：2015-11-03
申请人： Timothy Schimmel , Jacques Cohen , Michael Cecchi
设计人： Timothy Schimmel , Jacques Cohen , Michael Cecchi

15. 发明申请

US20080248563A1 Specimen container for the micro manipulation and biopsy in in-vitro fertilization 审中-公开
标题翻译：用于体外受精微量操作和活检的标本容器
公开(公告)号：US20080248563A1
公开(公告)日：2008-10-09
申请号：US11732187
申请日：2007-04-04
申请人： Michael D. Cecchi , Jacques Cohen , Tim Shimmel
发明人： Michael D. Cecchi , Jacques Cohen , Tim Shimmel
IPC分类号： C12M1/28
CPC分类号： C12M21/06 , C12M23/12
摘要： A container or dish is useful for the micro manipulation, micro injection, biopsy and fertilization in oocyte and embryo manipulation and culturing. The dish allows the user to more readily perform procedures used to fertilize oocytes by Intracytoplasmic sperm injection (ICSI) or other procedures, biopsy embryos and perform additional procedures for use in assisted reproductive techniques (ART), human reproduction and in-vitro fertilization (IVF). The invention allows ease in use, the reduction in the number of micro tools used in the procedure, as opposed to conventional dishes and procedures, and will allow the user to more readily locate the oocytes and embryos to be handled and worked on. The invention will add repetitiveness, consistency, improve efficacy and ease of procedure and may improve outcomes. The invention will also give the user a more ergonomically practical dish for these types of procedures and related protocols. Specimens other than oocytes and embryos can be treated in the container by means of micro manipulation.
摘要翻译：容器或皿可用于卵母细胞的微操作，微注射，活检和受精以及胚胎操纵和培养。该菜允许用户更容易地通过胞质内精子注射（ICSI）或其他程序，活检胚胎执行用于施肥卵母细胞的程序，并执行用于辅助生殖技术（ART），人类繁殖和体外受精（IVF）的附加程序）。与常规菜肴和程序相反，本发明允许易于使用，减少在该方法中使用的微型工具的数量，并且将允许使用者更容易地定位待处理和加工的卵母细胞和胚胎。本发明将增加重复性，一致性，提高疗效和易操作性，并可改善疗效。本发明还将为用户提供这些类型的程序和相关协议的更符合人体工程学的实用菜肴。可以通过微操作在容器中处理卵母细胞和胚胎以外的样品。

16. 发明授权

US06623956B1 Embryo-culturing incubator assembly 有权
标题翻译：胚胎培养箱组装
公开(公告)号：US06623956B1
公开(公告)日：2003-09-23
申请号：US09693595
申请日：2000-10-20
申请人： Michael D. Cecchi , Jacques Cohen , Timothy Schimmel , Monica Mezezi
发明人： Michael D. Cecchi , Jacques Cohen , Timothy Schimmel , Monica Mezezi
IPC分类号： C12M100
CPC分类号： C12M41/14 , C12M21/06 , C12M23/48 , Y10S435/809
摘要： An embryo culturing incubator is provided with a shelf configuration that enhances air circulation inside of the incubator, and enables unimpeded access to the individual embryo culture plates that are disposed on the shelves in the incubator. The incubator has an access door which allows one contact with the embryo culture plates on the incubator shelves. The shelves are configured so as to provide a useful free space between the inner surface of the incubator door and the frontal surface of the shelves in the incubator. The size of the free space allows a technician to reach all of the culture plates in the incubator without interfering with other culture plates in the incubator. The free space also allows for exemplary air circulation inside of the incubator.
摘要翻译：胚胎培养培养箱具有能够增强培养箱内的空气循环的架子结构，能够不受阻碍地进入设置在培养箱的架子上的各个胚胎培养板。培养箱具有进入门，允许与培养箱上的胚胎培养板接触。搁架被构造成在孵化器门的内表面和培养箱中的货架的前表面之间提供有用的自由空间。自由空间的大小允许技术人员到达孵化器中的所有培养皿，而不会干扰培养箱中的其他培养皿。自由空间还允许培养箱内部示例性的空气循环。

17. 发明授权

US6132952A Storage system comprising an emptied zona pellucida and spermatozoa placed therein and a method of cryopreservation 失效
标题翻译：包含置于其中的空的透明带和精子的储存系统和一种低温保存方法
公开(公告)号：US6132952A
公开(公告)日：2000-10-17
申请号：US88392
申请日：1998-06-01
申请人： Jacques Cohen , Steen Willadsen
发明人： Jacques Cohen , Steen Willadsen
IPC分类号： A01N1/02 , A61B17/43
CPC分类号： A01N1/0231 , A01N1/02 , A61B17/43
摘要： A procedure for cryopreservation and efficient post-thaw recovery of a single or few cells, preferably human spermatozoa, their precursor cells or blastomeres, which is achieved by injecting cells into cell-free mammalian zonae pellucidae. The cells may also be of any other type where there are provided a relatively small number of valuable cells which must be retrieved. The zona pellucida may be cryopreserved with the cells contained therein. The method involves a combination of physical micromanipulation procedures and cryoprotectant mediated cryopropreservation. The zonae may be tracked by positioning them into straws between two small air bubbles prior to preservation. There is a high recovery of zonae and sperm within the zonae, and a high fertilization rate of the sperm using ICSI after recovery.
摘要翻译：通过将细胞注射到无细胞的哺乳动物透明带中来实现的用于冷冻保存和有效解冻后的单个或少数细胞，优选人精子，其前体细胞或卵裂球的方法。细胞也可以是任何其它类型的细胞，其中提供了必须检索的相对少量的有价值的细胞。透明带可以与其中包含的细胞冷冻保存。该方法涉及物理显微操作程序和冷冻保护剂介导的冷冻保存的组合。在保存之前，可以通过将它们放置在两个小气泡之间的吸管中来跟踪该区域。 zonae中的zonae和精子恢复率高，恢复后使用ICSI的精子受精率高。

18. 发明授权

US4223456A Shoe sole assembly 失效
标题翻译：鞋底组装
公开(公告)号：US4223456A
公开(公告)日：1980-09-23
申请号：US1190
申请日：1979-01-05
申请人： Jacques Cohen
发明人： Jacques Cohen
IPC分类号： A43B7/08 , A43B13/20 , A43B13/04
CPC分类号： A43B7/08 , A43B13/206
摘要： A shoe sole assembly of a resilient material body portion provided with absorbers energy formed as part of the body portion. The energy absorbers are defined by vertically disposed, spaced apart cell members extending from an upper surface to beyond a lower surface of the body portion to provide a bounded space. Each cell member is formed of an hollow compartment extending in part for the thickness of the body portion with its length depending upon the sole profile, the first end of the respective compartments at the upper surface of the sole body portion is initially open ended; whereas a hollow protuberance structure is at the lower surface of the body portion to enclose each cylindrical compartment at a second end. The protuberances extend beyond the lower surface to form contact areas with the ground surface when the sole assembly is used as part of a shoe. The compartments are separated from one another by adjacent lands which at the upper sole surface provide define respective substrate areas for the disposition of an inner sole thereon, so to seal each cell member with a volume of air trapped in the bounded space of the compartment and associated hollow protuberance structure.
摘要翻译：一种弹性材料主体部分的鞋底组件，其具有形成为主体部分的一部分的吸收体能量。能量吸收器由垂直设置的间隔开的细胞构件限定，其从上表面延伸到主体部分的下表面之外，以提供有界空间。每个单元构件由中空隔室形成，其部分地为主体部分的厚度延伸，其长度取决于底部轮廓，在鞋底主体部分的上表面处的相应隔间的第一端初始是开放的; 而中空的突起结构位于主体部分的下表面，以在第二端处包围每个圆柱形隔室。当鞋底组件用作鞋的一部分时，突起延伸超出下表面以与地面形成接触区域。隔室通过相邻的平台彼此分开，在上部鞋底表面提供限定相应的衬底区域，用于在其上设置内部鞋底，从而密封每个细胞构件，其中空气被捕获在隔室的有界空间中，相关的中空突起结构。

19. 发明申请

US20180112164A1 Device for Observation, Imaging and Uninterrupted Culturing of Embryos and Cells 审中-公开
公开(公告)号：US20180112164A1
公开(公告)日：2018-04-26
申请号：US15334953
申请日：2016-10-26
申请人： Michael D. Cecchi , Timothy Schimmel , Jacques Cohen , Monica Mezezi , Michael Ryan Cecchi
发明人： Michael D. Cecchi , Timothy Schimmel , Jacques Cohen , Monica Mezezi , Michael Ryan Cecchi
IPC分类号： C12M1/00 , C12M3/00 , C12M1/34 , G01N21/84
CPC分类号： G01N21/84 , C12M23/22 , C12M23/48 , C12M23/50 , C12M31/02 , C12M41/14 , C12M41/34 , C12M41/36
摘要： A device for the observation, imaging and uninterrupted culturing of embryos and cells, the device comprising: an incubator; a mixing box located above the incubator; gas sensors located in the mixing box; solenoid valves attached to the mixing box; a first clear panel located on the top of the incubator, and configured to allow one to see into the incubator; a first light source located above the first clear panel, and the first light source configured to provide light into the incubator; a door attached to the incubator, the door configured to provide access to the inside of the incubator, and the door located below the access door; a rotatable platter located below the incubator, the rotatable platter comprising: a first insert configured to removably sit on the platter; a first culture dish configured to removably sit in the first insert; a second insert configured to removably sit on the platter; a second culture dish configured to removably sit in the second insert; a first moveable camera located under the rotatable platter, and configured to view a culture dish located generally directly above the first moveable camera, and the first moveable camera generally in alignment with the first light source; a computer in communication with the first moveable camera, rotatable platter, sensors, solenoid valves, touchscreen display, and light source. A device for the observation, imaging and uninterrupted culturing of embryos and cells, the device comprising: a top portion, the top portion comprising: a housing; a horizontal surface on the housing; an angled surface abutting the horizontal surface; an access door located generally on the housing; a first heating surface located on the horizontal surface; a touch screen display located on the angled surface; a middle portion abutting the top portion; the middle portion comprising: an incubator; a mixing box located above the incubator; gas sensors located in the mixing box; solenoid valves attached to the mixing box; a first clear panel located on the top of the incubator, and configured to allow one to see into the incubator; a first light source located above the first clear panel, and the first light source configured to provide light into the incubator; a door attached to the incubator, the door configured to provide access to the inside of the incubator, and the door located below the access door; a bottom portion abutting the middle portion, the bottom portion comprising: a rotatable platter, the rotatable platter comprising: a first insert configured to removably sit on the platter; a first culture dish configured to removably sit in the first insert; a second insert configured to removably sit on the platter; a second culture dish configured to removably sit in the second insert; a first moveable camera located under the rotatable platter, and configured to view a culture dish located generally directly above the first moveable camera, and the first moveable camera generally in alignment with the first light source; a computer in communication with the camera, rotatable platter, sensors, solenoid valves, touchscreen display, and light source.

20. 发明申请

US20170002306A1 Gas Recirculation System for an Incubated Controlled Environment 审中-公开
公开(公告)号：US20170002306A1
公开(公告)日：2017-01-05
申请号：US14789591
申请日：2015-07-01
申请人： Michael Cecchi , Timothy Schimmel , Monica Mezzezi , Jacques Cohen , Michael R. Cecchi
发明人： Michael Cecchi , Timothy Schimmel , Monica Mezzezi , Jacques Cohen , Michael R. Cecchi
IPC分类号： C12M1/00 , C12M1/34
CPC分类号： C12M41/34 , C12M41/14
摘要： A gas recirculation system for an incubated controlled environment, the system comprising: a first mixing box; a second mixing box in fluid communication with the first mixing box; a manifold in fluid communication with the second mixing box; a plurality of incubation chambers in fluid communication with the manifold; a return manifold in fluid communication with the plurality of incubation chambers and in fluid communication with the first mixing box. A gas recirculation system for an incubated controlled environment, the system comprising: a first mixing box; a second mixing box in fluid communication with the first mixing box; an inlet port in fluid communication with the second mixing box; a plurality of incubation chamber inlet ports; an incubation chamber in fluid communication with the plurality of incubation chamber inlet ports; a plurality of incubation chamber outlet ports in fluid communication with the incubation chamber; a check valve in fluid communication with the plurality of incubation outlet ports, and in fluid communication with the second mixing box. A gas recirculation system for an incubated controlled environment, the system comprising: a pump; a filter in fluid communication with the pump; a sensor box in fluid communication with the filter; a pH sensor box in fluid communication with the sensor box, the pH sensor box comprising a container with embryo culture media and a pH sensor configured to determine the pH of the embryo culture media; a regulator in fluid communication with the pH sensor box; an embryo chamber in fluid communication with the regulator; a second pH sensor box in fluid communication with the regulator, the pH sensor box comprising a container with embryo culture media and a pH sensor configured to determine the pH of the embryo culture media. A gas recirculation system for an incubated controlled environment, the system comprising: a means for monitoring the O2 and CO2 concentration of gases in the incubated controlled environment; a means for injecting CO2, N2, and/or O2 into the incubated controlled environment via a mixing box; a means for adjusting the CO2, N2, and O2 levels in the incubated controlled environment. A gas recirculation system for an incubated controlled environment of claim 16, further comprising: a means for introducing CO2, N2, and/or O2 into the incubated environment in order to maintain about 5% of CO2; about 90% of N2; and/or about 5% of O2 in the incubated environment.

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