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    • 18. 发明授权
    • Thrombin-binding substance and process for its preparation
    • 凝血酶结合物质及其制备方法
    • US5047503A
    • 1991-09-10
    • US72051
    • 1987-07-10
    • Nobuo AokiShigeru KimuraMasami Shiratsuchi
    • Nobuo AokiShigeru KimuraMasami Shiratsuchi
    • A61K38/00C07K14/745C07K16/40
    • C07K14/7455C07K16/40A61K38/00Y10S530/834
    • Thrombin-binding substances are obtained by fractionating human urine by ion-exchange chromatography, affinity chromatography using a thrombin-bound carrier, immune adsorption column chromatography, gel filtration, and/or molecular-weight fractionation. One of the substances has a molecular weight of 46,500.+-.6,000 in reduced condition and 39,000.+-.10,000 in unreduced condition by SDS PAGE and an isoelectric point at pH 5.0-5.3, while the other has a molecular weight of 40,000.+-.8,000 in reduced condition and 31,000.+-.10,000 in unreduced condition by SDS PAGE and an isoelectric point at pH 4.9-5.7. They have strong affinity to thrombin. They are capable of promoting the thrombin catalyzed activation of protein C and prolong clotting time. They are stable to denaturing agents (urea and sodium dodecylsulfate).
    • 凝血酶结合物质通过离子交换色谱法,使用凝血酶结合载体的亲和层析,免疫吸附柱色谱法,凝胶过滤和/或分子量分级法分级人尿获得。 其中一种物质在还原条件下的分子量为46,500 +/- 6,000,通过SDS PAGE分析为39,000 +/- 10,000,在pH 5.0-5.3时为等电点,而另一种的分子量为40,000+ / -8,000的还原条件和31,000 +/- 10,000的未降解条件下通过SDS PAGE和等电点在pH 4.9-5.7。 它们对凝血酶具有很强的亲和力。 它们能够促进凝血酶催化蛋白C的活化并延长凝血时间。 它们对变性剂(尿素和十二烷基硫酸钠)是稳定的。