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11. 发明授权

US06451261B1 Multi-well microfiltration apparatus 失效
公开(公告)号：US06451261B1
公开(公告)日：2002-09-17
申请号：US09565673
申请日：2000-05-04
申请人： Kevin S. Bodner , Alfred P. Madden , Joseph Jackson , Jason H. Halsey , Mark T. Reed , Ward Frye , Mark F. Oldham , Stephen E. Moring , Jon Hoshizaki
发明人： Kevin S. Bodner , Alfred P. Madden , Joseph Jackson , Jason H. Halsey , Mark T. Reed , Ward Frye , Mark F. Oldham , Stephen E. Moring , Jon Hoshizaki
IPC分类号： B01L300
CPC分类号： B01L3/5025 , B01D61/18 , B01L3/50255 , B01L2400/049 , G01N35/0099 , G01N35/028 , G01N35/1074 , G01N2035/00485 , G01N2035/102 , Y10T436/25
摘要： The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.

12. 发明授权

US6159368A Multi-well microfiltration apparatus 有权
标题翻译：多孔微滤装置
公开(公告)号：US6159368A
公开(公告)日：2000-12-12
申请号：US182946
申请日：1998-10-29
申请人： Stephen E. Moring , Kevin S. Bodner , Jason H. Halsey , Jon Hoshizaki , Joseph Jackson , Alfred P. Madden , Mark F. Oldham , Mark T. Reed
发明人： Stephen E. Moring , Kevin S. Bodner , Jason H. Halsey , Jon Hoshizaki , Joseph Jackson , Alfred P. Madden , Mark F. Oldham , Mark T. Reed
IPC分类号： G01N1/00 , B01D29/00 , B01D35/02 , B01D53/22 , B01D61/18 , B01L3/00 , C12N15/09 , G01N35/00 , G01N35/02 , G01N35/10 , B01D63/00
CPC分类号： B01L3/5025 , B01D61/18 , B01L3/50255 , G01N35/0099 , B01L2400/049 , G01N2035/00485 , G01N2035/102 , G01N35/028 , G01N35/1074 , Y10T436/25
摘要： The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with is the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.
摘要翻译：本发明提供了多孔板和柱阵列，其中可以分析和/或处理样品（例如，含有目标核酸的细胞裂解物，例如RNA）。在一个实施方案中，微滤装置是多层结构，包括（i）具有可以放置样品的小柱阵列的柱板，（ii）设置在每个小柱中的离散过滤元件，（iii）滴液导向器板具有相应的滴液导向器阵列，滤液可以通过其排出;以及（iv）接收孔板，其具有滤液能够流过的相应阵列的接收孔。本发明提供了相对简单的多孔微过滤装置，并且克服了许多与以下问题有关的问题：（i）由于穿过公共过滤片芯线而引起的交叉污染或（ii）单个过滤元件在大量死体积内捕获样品成分。此外，本发明提供了多孔微过滤装置，其充分地支撑设置在井中的分立的过滤元件，而不产生实质的优先流动。另外，本发明提供了多孔微量过滤装置，其避免了由于气溶胶形成，悬挂液滴和/或飞溅而导致的交叉污染。本发明的其它公开的特征提供了分别收集在孔阵列中的滤液样品的自动覆盖或热封。

13. 发明授权

US06783732B2 Apparatus and method for avoiding cross-contamination due to pendent drops of fluid hanging from discharge conduits 有权
标题翻译：用于避免由排放管道悬挂的流体悬垂液滴引起的交叉污染的装置和方法
公开(公告)号：US06783732B2
公开(公告)日：2004-08-31
申请号：US10199743
申请日：2002-07-19
申请人： Alfred P Madden , Jon Hoshizaki , Jason H. Halsey , Stephen E. Moring , Mark F. Oldham , Kevin S. Bodner
发明人： Alfred P Madden , Jon Hoshizaki , Jason H. Halsey , Stephen E. Moring , Mark F. Oldham , Kevin S. Bodner
IPC分类号： G01N3500
CPC分类号： B01L3/5025 , B01D61/18 , B01L3/50255 , B01L2400/049 , G01N35/0099 , G01N35/028 , G01N35/1074 , G01N2035/00485 , G01N2035/102 , Y10T436/25
摘要： The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.
摘要翻译：本发明提供了多孔板和柱阵列，其中可以分析和/或处理样品（例如，含有目标核酸的细胞裂解物，例如RNA）。在一个实施方案中，微滤装置是多层结构，包括（i）具有可以放置样品的小柱阵列的柱板，（ii）设置在每个小柱中的离散过滤元件，（iii）滴液导向器板具有相应的滴液导向器阵列，滤液可以通过其排出;以及（iv）接收孔板，其具有滤液能够流过的相应阵列的接收孔。本发明提供了相对简单的多孔微量过滤装置，并且克服了许多与先前布置有关的问题，这些问题涉及（i）由于芯毛穿过公共过滤片引起的交叉污染或（ii）截留的单个过滤器元件大量死体积的样品成分。此外，本发明提供了多孔微过滤装置，其充分地支撑设置在井中的分立的过滤元件，而不产生实质的优先流动。此外，本发明提供了多孔微滤装置，其避免了由于气溶胶形成，悬垂滴和/或飞溅而导致的交叉污染。本发明的其它公开的特征提供了分别收集在孔阵列中的滤液样品的自动覆盖或热封。

14. 发明授权

US06506343B1 Multi-well microfiltration apparatus and method for avoiding cross-contamination 有权
标题翻译：多孔微滤装置及避免交叉污染的方法
公开(公告)号：US06506343B1
公开(公告)日：2003-01-14
申请号：US09565202
申请日：2000-05-04
申请人： Kevin S. Bodner , Alfred P. Madden , Jason H. Halsey , Mark F. Oldham , Stephen E. Moring , Jon Hoshizaki
发明人： Kevin S. Bodner , Alfred P. Madden , Jason H. Halsey , Mark F. Oldham , Stephen E. Moring , Jon Hoshizaki
IPC分类号： B01D3500
CPC分类号： B01L3/5025 , B01D61/18 , B01L3/50255 , B01L2400/049 , G01N35/0099 , G01N35/028 , G01N35/1074 , G01N2035/00485 , G01N2035/102 , Y10T436/25
摘要： The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.
摘要翻译：本发明提供了多孔板和柱阵列，其中可以分析和/或处理样品（例如，含有目标核酸的细胞裂解物，例如RNA）。在一个实施方案中，微滤装置是多层结构，包括（i）具有可以放置样品的小柱阵列的柱板，（ii）设置在每个小柱中的离散过滤元件，（iii）滴液导向器板具有相应的滴液导向器阵列，滤液可以通过其排出;以及（iv）接收孔板，其具有滤液能够流过的相应阵列的接收孔。本发明提供了相对简单的多孔微量过滤装置，并且克服了许多与先前布置有关的问题，这些问题涉及（i）由于芯毛穿过公共过滤片引起的交叉污染或（ii）截留的单个过滤器元件大量死体积的样品成分。此外，本发明提供了多孔微过滤装置，其充分地支撑设置在井中的分立的过滤元件，而不产生实质的优先流动。此外，本发明提供了多孔微滤装置，其避免了由于气溶胶形成，悬垂滴和/或飞溅而导致的交叉污染。本发明的其它公开的特征提供了分别收集在孔阵列中的滤液样品的自动覆盖或热封。

15. 发明授权

US06338802B1 Multi-well microfiltration apparatus 有权
标题翻译：多孔微滤装置
公开(公告)号：US06338802B1
公开(公告)日：2002-01-15
申请号：US09565566
申请日：2000-05-04
申请人： Kevin S. Bodner , Alfred P. Madden , Joseph Jackson , Jason H. Halsey , Mark T. Reed , Ward Frye , Mark F. Oldham , Stephen E. Moring , Jon Hoshizaki
发明人： Kevin S. Bodner , Alfred P. Madden , Joseph Jackson , Jason H. Halsey , Mark T. Reed , Ward Frye , Mark F. Oldham , Stephen E. Moring , Jon Hoshizaki
IPC分类号： B01D6100
CPC分类号： B01L3/5025 , B01D61/18 , B01L3/50255 , B01L2400/049 , G01N35/0099 , G01N35/028 , G01N35/1074 , G01N2035/00485 , G01N2035/102 , Y10T436/25
摘要： The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.
摘要翻译：本发明提供了多孔板和柱阵列，其中可以分析和/或处理样品（例如，含有目标核酸的细胞裂解物，例如RNA）。在一个实施方案中，微滤装置是多层结构，包括（i）具有可以放置样品的小柱阵列的柱板，（ii）设置在每个小柱中的离散过滤元件，（iii）滴液导向器板具有相应的滴液导向器阵列，滤液可以通过其排出;以及（iv）接收孔板，其具有滤液能够流过的相应阵列的接收孔。本发明提供了相对简单的多孔微量过滤装置，并且克服了许多与先前布置有关的问题，这些问题涉及（i）由于芯毛穿过公共过滤片引起的交叉污染或（ii）截留的单个过滤器元件大量死体积的样品成分。此外，本发明提供了多孔微过滤装置，其充分地支撑设置在井中的分立的过滤元件，而不产生实质的优先流动。此外，本发明提供了多孔微滤装置，其避免了由于气溶胶形成，悬垂滴和/或飞溅而导致的交叉污染。本发明的其它公开的特征提供了分别收集在孔阵列中的滤液样品的自动覆盖或热封。

16. 发明授权

US5928907A System for real time detection of nucleic acid amplification products 失效
标题翻译：核酸扩增产物实时检测系统
公开(公告)号：US5928907A
公开(公告)日：1999-07-27
申请号：US752973
申请日：1996-12-02
申请人： Timothy M. Woudenberg , Kevin S. Bodner , Charles R. Connell , Alan M. Ganz , Lincoln J. McBride , Paul G. Saviano , John Shigeura , David H. Tracy , Eugene F. Young , Linda G. Lee
发明人： Timothy M. Woudenberg , Kevin S. Bodner , Charles R. Connell , Alan M. Ganz , Lincoln J. McBride , Paul G. Saviano , John Shigeura , David H. Tracy , Eugene F. Young , Linda G. Lee
IPC分类号： C12N15/09 , C12P19/34 , C12Q1/68 , G01N21/03 , G01N21/64 , G01N21/75 , G01N21/78 , G01N33/50 , G01N33/58
CPC分类号： G01N21/6428 , C12Q1/6818 , C12Q1/6851 , C12Q1/686 , G01N21/0303 , G01N2021/6484
摘要： A system is provided for carrying out real time fluorescence-based measurements of nucleic acid amplification products. In a preferred embodiment of the invention, an excitation beam is focused into a reaction mixture through a surface, the reaction mixture containing (i) a first fluorescent indicator capable of generating a first fluorescent signal whose intensity is proportional to the amount of an amplification product in the volume of the reaction mixture illuminated by the excitation beam and (ii) a second fluorescent indicator homogeneously distributed throughout the reaction mixture capable of generating a second fluorescent signal proportional to the volume of reaction mixture illuminated by the excitation beam. Preferably, the excitation beam is focused into the reaction mixture by a lens through a portion of a wall of a closed reaction chamber containing the reaction mixture. The same lens is used to collect the first and second fluorescent signals generated by the first and second fluorescent indicators, respectively, in response to the excitation beam. The ratio of the fluorescent intensities of the first and second fluorescent signals provides a stable quantitative indicator of the amount of amplification product synthesized in the course of the amplification reaction.
摘要翻译：提供了一种用于进行核酸扩增产物的实时基于荧光的测量的系统。在本发明的优选实施方案中，激发光束通过表面聚焦到反应混合物中，反应混合物含有（i）第一荧光指示剂，其能够产生第一荧光信号，其强度与扩增产物的量成比例在由激发束照射的反应混合物的体积中和（ii）均匀分布在整个反应混合物中的第二荧光指示器，其能够产生与由激发束照射的反应混合物的体积成比例的第二荧光信号。优选地，激发光束通过透镜通过包含反应混合物的封闭反应室的壁的一部分聚焦到反应混合物中。相同的透镜被用于响应于激发光束分别收集由第一和第二荧光指示器产生的第一和第二荧光信号。第一和第二荧光信号的荧光强度比提供了在扩增反应过程中合成的扩增产物的量的稳定的定量指标。

17. 发明授权

US6015674A Apparatus and method for detecting nucleic acid amplification products 失效
标题翻译：用于检测核酸扩增产物的装置和方法
公开(公告)号：US6015674A
公开(公告)日：2000-01-18
申请号：US46114
申请日：1998-03-20
申请人： Timothy M. Woudenberg , Kevin S. Bodner , Charles R. Connell , John Shigeura , David H. Tracy , Eugene F. Young
发明人： Timothy M. Woudenberg , Kevin S. Bodner , Charles R. Connell , John Shigeura , David H. Tracy , Eugene F. Young
IPC分类号： C12N15/09 , C12P19/34 , C12Q1/68 , G01N21/03 , G01N21/64 , G01N21/75 , G01N21/78 , G01N33/50 , G01N33/58
CPC分类号： G01N21/6428 , C12Q1/6818 , C12Q1/6851 , C12Q1/686 , G01N21/0303 , G01N2021/6484
摘要： An apparatus is provided which includes a sample holder for holding a reaction chamber which includes an optical interface, a fiber optic cable for delivering an excitation beam to a sample housed within the reaction chamber and for receiving light emitted by the sample, and a lens co-axially disposed with the fiber optic cable and positioned outside the reaction chamber for focusing the excitation beam through the optical interface and within a volume of the sample and for collecting and transmitting to the fiber optic cable light emitted within the volume of the sample.
摘要翻译：提供了一种装置，其包括用于保持包括光学界面的反应室的样品保持器，用于将激发光束传送到容纳在反应室内的样品并用于接收由样品发射的光的光纤电缆，以及透镜组与光纤电缆同轴设置并且定位在反应室外部，用于将激发光束聚焦通过光学界面并在样品的体积内，并用于收集和传输在样品体积内发射的光纤光缆。

18. 发明授权

US06805842B1 Repuncturable self-sealing sample container with internal collapsible bag 失效
标题翻译：具有内部可折叠袋的可重复自密封样品容器
公开(公告)号：US06805842B1
公开(公告)日：2004-10-19
申请号：US09976628
申请日：2001-10-12
申请人： Kevin S. Bodner , Tyler A. Palmer , Pejman Ghanouni
发明人： Kevin S. Bodner , Tyler A. Palmer , Pejman Ghanouni
IPC分类号： B01L300
CPC分类号： B01L3/505 , B01L3/0217 , B01L3/50825 , B01L3/5085 , B01L2200/142 , B01L2300/044 , B01L2300/047 , B01L2300/0672 , B01L2300/0854 , B01L2400/0478
摘要： A sample container for minimizing evaporation of a contained volume of sample includes a container housing, a repuncturable self-sealing membrane, and a collapsible sample bag. The container housing includes an open end and a hollow interior region. The repuncturable self-sealing membrane configured to self-seal after repeated punctures is engaged in the open end of the container housing and includes an exterior surface exposed to the external environment and an interior surface oriented toward the hollow interior region of the container housing. The collapsible sample bag includes a proximate end that is permanently attached to the interior surface of the repuncturable self-sealing membrane.
摘要翻译：用于最小化容纳体积的样品的蒸发的样品容器包括容器壳体，可重复的自密封膜和可折叠样品袋。容器壳体包括开口端和中空的内部区域。构造为在重复穿刺之后自密封的可重复使用的自密封膜被接合在容器壳体的开口端中，并且包括暴露于外部环境的外表面和朝向容器壳体的中空内部区域定向的内表面。可折叠的样品袋包括永久地附着到可再次自密封膜的内表面的近端。

19. 发明授权

US06461808B1 Pipette-loaded bioassay assembly for detecting molecular or cellular events 失效
标题翻译：用于检测分子或细胞事件的移液器生物测定组件
公开(公告)号：US06461808B1
公开(公告)日：2002-10-08
申请号：US09880746
申请日：2001-06-12
申请人： Kevin S. Bodner , Andrew P. Sandham
发明人： Kevin S. Bodner , Andrew P. Sandham
IPC分类号： C12Q100
CPC分类号： G01N22/00 , G01N33/54373 , Y10T436/11 , Y10T436/119163
摘要： A pipette-loaded bioassay includes a measurement probe and a pipette tip. The measurement probe includes a probe head configured to launch an incident test signal and a connecting end configured to receive the incident test signal from a signal source. The pipette tip includes a sample interrogation region which is electromagnetically coupled to the probe head, the sample interrogation region configured to retain a plug of sample solution and constructed from a material which is substantially transparent to the incident test signal. The incident test signal electromagnetically couples through the sample interrogation region and to the molecular or cellular events occurring within the sample solution. The interaction of the incident test signal with the molecular or cellular events produces a modulated test signal which can be recovered and used to identify the molecular or cellular events occurring in a subsequently tested sample.
摘要翻译：移液管装载的生物测定包括测量探针和移液管尖。测量探头包括被配置为发射入射测试信号的探头和被配置为从信号源接收入射测试信号的连接端。移液管尖端包括电磁耦合到探针头的样品询问区域，样品询问区域被配置为保持样品溶液塞并由对入射的测试信号基本透明的材料构成。事件测试信号通过样品询问区域电磁耦合到样品溶液中发生的分子或细胞事件。事件测试信号与分子或细胞事件的相互作用产生调制的测试信号，其可以被回收并用于鉴定随后测试的样品中发生的分子或细胞事件。

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