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11. 发明申请

US20090325164A1 MICROELECTRONIC SENSOR DEVICE FOR DNA DETECTION 有权
标题翻译：用于DNA检测的微电子传感器装置
公开(公告)号：US20090325164A1
公开(公告)日：2009-12-31
申请号：US12303448
申请日：2007-05-24
申请人： Erik Robert Vossenaar , Marc Wilhelmus Gisbert Ponjee , Hendrik Roelof Stapert , Mark Thomas Johnson
发明人： Erik Robert Vossenaar , Marc Wilhelmus Gisbert Ponjee , Hendrik Roelof Stapert , Mark Thomas Johnson
IPC分类号： C12Q1/68 , C12M1/00
CPC分类号： G01N21/6454 , B01L3/5027 , B01L3/50851 , B01L7/525 , B01L7/54 , B01L2200/147 , B01L2300/0636 , B01L2300/0819 , B01L2300/1822 , B01L2300/1827 , C12Q1/6825 , G01N21/0332 , G01N21/6428 , G01N2021/6439 , C12Q2565/1015 , C12Q2527/101 , C12Q2527/107
摘要： The invention relates to a microelectronic sensor device and a method for the investigation of biological target substances (20), for example oligonucleotides like DNA fragments. In one embodiment, the device comprises a reaction surface (RS) to which target specific reactants (10) are attached and which lies between a sample chamber (SC) and an array of selectively controllable heating elements (HE). The temperature profile in the sample chamber (SC) can be controlled as desired to provide for example conditions for a PCR and/or for a controlled melting of hybridizations. The reactant (10) and/or the target substance (20) comprises a label (12) with an observable property, like fluorescence, that changes if the target substance (20) is bound to the reactant (10), said property being detected by an array of sensor elements, for example photosensors (SE). The fluorescence of the label (12) may preferably be transferred by FRET to a different fluorescent label (22) or quenched if the target substance (20) is bound.
摘要翻译：本发明涉及一种微电子传感器装置和一种用于研究生物靶物质（20）的方法，例如DNA片段等寡核苷酸。在一个实施方案中，所述装置包括反应表面（RS），目标特异性反应物（10）附着到所述反应表面（RS），并且位于样品室（SC）和选择性可控制的加热元件（HE）阵列之间。样品室（SC）中的温度曲线可以根据需要进行控制，以提供例如PCR和/或受控熔化杂交的条件。反应物（10）和/或目标物质（20）包含具有可观察性质（如荧光）的标记物（12），如果目标物质（20）与反应物（10）结合，则其变化，所述性质被检测通过传感器元件阵列，例如光电传感器（SE）。标记物（12）的荧光优选通过FRET转移到不同的荧光标记物（22），或者如果靶物质（20）被结合则被淬灭。

12. 发明申请

US20090269858A1 METHOD OF DETERMINING THE CONCENTRATION OF AN ANALYTE USING ANALYTE SENSOR MOLECULES COUPLED TO A POROUS MEMBRANE 审中-公开
标题翻译：使用与多孔膜结合的分析仪传感器分子来确定分析仪浓度的方法
公开(公告)号：US20090269858A1
公开(公告)日：2009-10-29
申请号：US12375769
申请日：2007-08-01
申请人： Gardiye Hewa Pattinige Kusalini Chamindie Punyadeera , Hendrik Roelof Stapert
发明人： Gardiye Hewa Pattinige Kusalini Chamindie Punyadeera , Hendrik Roelof Stapert
IPC分类号： G01N33/548 , G01N33/545
CPC分类号： G01N33/54366 , G01N33/548
摘要： The present invention relates to a method of determining the concentration of an analyte in a sample and/or the binding kinetics of an analyte to an analyte sensor molecule. For this purpose the invention relies on detecting the interaction between the analyte and the analyte sensor molecule with the latter being physically adsorbed to a solid, porous support which is in the form of a micro array. In a preferred embodiment, the method may be operated in repeated cycles.
摘要翻译：本发明涉及确定样品中分析物的浓度和/或分析物与分析物传感器分子的结合动力学的方法。为此目的，本发明依赖于检测分析物和分析物传感器分子之间的相互作用，后者被物理吸附到呈微阵列形式的固体多孔载体上。在优选实施例中，该方法可以重复循环操作。

13. 发明申请

US20090203544A1 METHOD AND USE OF A PRINTER DEVICE FOR PRODUCING BIOLOGICAL TEST ARRAYS 审中-公开
标题翻译：用于生产生物测试阵列的打印机的方法和用途
公开(公告)号：US20090203544A1
公开(公告)日：2009-08-13
申请号：US12306289
申请日：2007-07-03
申请人： Anke Pierik , Johan Frederik Dijksman , Hendrik Roelof Stapert , Roy Gerardus Franciscus Antonius Verbeek , Aleksey Kolesnychenko
发明人： Anke Pierik , Johan Frederik Dijksman , Hendrik Roelof Stapert , Roy Gerardus Franciscus Antonius Verbeek , Aleksey Kolesnychenko
IPC分类号： C40B60/14 , C40B50/14 , C40B40/00
CPC分类号： G01N35/1074 , B01J19/0046 , B01J2219/0036 , B01J2219/00378 , B01J2219/00527 , B01J2219/00659 , B01J2219/00693 , G01N35/1016 , G01N2035/1076
摘要： The invention relates to a printer device for producing biological test arrays by depositing an array of biofluids onto a substrate. The invention further relates to the use of such a device in the production of biological test arrays. The invention also relates to a method for producing a biological test array. The invention moreover relates to a biological test array. The method according to the invention is failure-proof, and results in biological test arrays of superior quality.
摘要翻译：本发明涉及一种用于通过将生物流体阵列沉积到基底上来生产生物测试阵列的打印机装置。本发明还涉及这种装置在生物测试阵列生产中的应用。本发明还涉及一种用于生产生物测试阵列的方法。本发明还涉及生物测试阵列。根据本发明的方法是无故障的，并且导致具有优良品质的生物测试阵列。

14. 发明授权

US09657338B2 Method for immobilizing nucleic acids on a support 有权
公开(公告)号：US09657338B2
公开(公告)日：2017-05-23
申请号：US13121206
申请日：2009-09-22
申请人： Anke Pierik , Hendrik Roelof Stapert
发明人： Anke Pierik , Hendrik Roelof Stapert
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6837 , C12Q2525/173
摘要： The present invention relates to a method for immobilizing nucleic acids on a support, comprising the provision of a nucleic acid with a stretch of nucleotides of only one basetype and the immobilization of said nucleic acid on a support by crosslinking by light, wherein said crosslinking by light is performed at a wavelength of about 300-500 nm, preferably at a wavelength of 365 nm. The present invention further relates to a method for the analysis of nucleic acids immobilized according to the invention, which comprises the hybridization of the immobilized nucleic acid with complementary and mismatch segments. Furthermore, the present invention relates to immobilized nucleic acids obtainable by the method of the invention, the use of accordingly immobilized nucleic acids for the production of nucleic acid arrays and a diagnostic kit, comprising an array of nucleic acids which are immobilized according to the present invention.

15. 发明授权

US08475145B2 Micro-fluidic systems based on actuator elements 有权
标题翻译：基于执行器元件的微流体系统
公开(公告)号：US08475145B2
公开(公告)日：2013-07-02
申请号：US11816537
申请日：2006-02-08
申请人： Jacob Marinus Jan Den Toonder , Menno Willem Jose Prins , Hendrik Roelof Stapert , Dirk Jan Broer
发明人： Jacob Marinus Jan Den Toonder , Menno Willem Jose Prins , Hendrik Roelof Stapert , Dirk Jan Broer
IPC分类号： F04B35/04
CPC分类号： F04D33/00 , B01F13/0059 , B01F13/0091 , B01F13/0827 , B01L3/502707 , B01L3/502746 , B01L2400/0484 , F04B19/006 , Y10T29/49236 , Y10T137/0391
摘要： The present invention provides micro-fluidic systems, a method for the manufacturing of such a micro-fluidic system and a method for controlling or manipulating a fluid flow through micro-channels of a such a micro-fluidic system. Herefore, an inner side of a wall of a microchannel is provided with actuator elements which can change shape and orientation as a response to an external stimulus. Through this change of shape and orientation the flow of a fluid through a microchannel may be controlled and manipulated.
摘要翻译：本发明提供微流体系统，用于制造这种微流体系统的方法以及用于控制或操纵通过这种微流体系统的微通道的流体流动的方法。因此，微通道壁的内侧设置有作为对外部刺激的响应而改变形状和取向的致动器元件。通过形状和取向的变化，流体通过微通道的流动可被控制和操纵。

16. 发明授权

US08039814B2 Luminescence sensor operating in reflection mode 有权
标题翻译：发光传感器以反射模式工作
公开(公告)号：US08039814B2
公开(公告)日：2011-10-18
申请号：US12158451
申请日：2006-12-19
申请人： Maarten Marinus Johannes Wilhelm Van Herpen , Derk Jan Wilfred Klunder , Hendrik Roelof Stapert
发明人： Maarten Marinus Johannes Wilhelm Van Herpen , Derk Jan Wilfred Klunder , Hendrik Roelof Stapert
IPC分类号： G01N21/64
CPC分类号： G01N21/648 , G01N21/6428
摘要： A luminescence sensor, such as a luminescence biosensor or a luminescence chemical sensor, includes a substrate having at least one aperture filled with a medium. The aperture has a first lateral dimension larger than the diffraction limit of excitation radiation in the medium, and a second lateral dimension smaller than the diffraction limit of the excitation radiation in the medium. A method is also provided for the detection of luminescence radiation, e.g. fluorescence radiation, generated by at least one optically variable molecule, e.g. fluorophore, in the at least one aperture. Beneficially, the excitation radiation is polarized to suppress the excitation radiation in the apertures. The luminescence sensor and the method are able to detect relatively low concentrations of optically variable molecules, e.g. fluorophores.
摘要翻译：诸如发光生物传感器或发光化学传感器的发光传感器包括具有填充有介质的至少一个孔的基板。该孔具有比介质中的激发辐射的衍射极限大的第一横向尺寸，以及小于介质中的激发辐射的衍射极限的第二横向尺寸。还提供了一种用于检测发光辐射的方法，例如，由至少一个光学可变分子产生的荧光辐射，例如。荧光团，在至少一个孔中。有利地，激发辐射被极化以抑制孔中的激发辐射。发光传感器和方法能够检测相对低浓度的光学可变分子，例如，荧光团

17. 发明申请

US20100019155A1 LUMINESCENCE SENSORS USING SUB-WAVELENGTH APERTURES OR SLITS 审中-公开
标题翻译：使用子波长波形或者倾斜的发光体传感器
公开(公告)号：US20100019155A1
公开(公告)日：2010-01-28
申请号：US11917947
申请日：2006-06-16
申请人： Derk Jan Wilfred Klunder , Maarten Marinus Johannes Wilhelm Van Herpen , Hendrik Roelof Stapert
发明人： Derk Jan Wilfred Klunder , Maarten Marinus Johannes Wilhelm Van Herpen , Hendrik Roelof Stapert
IPC分类号： G01N21/64 , G01N21/63
CPC分类号： G01N21/6428 , B82Y15/00 , G01N21/648 , G01N33/54373
摘要： The present invention provide a qualitative or quantitative luminescence sensor, for example a bio sensor or chemical sensor, using sub-wavelength aperture or slit structures, i.e. using apertures or slit structures having a smallest dimension smaller than the wavelength of the excitation radiation in the medium that fills the aperture or slit structure. The invention furthermore provides a method for the detection of luminescence radiation generated by one or more luminophores present in aperture or slit structure in such a luminescence sensor.
摘要翻译：本发明提供定性或定量发光传感器，例如使用亚波长孔径或狭缝结构的生物传感器或化学传感器，即使用具有小于介质中的激发辐射的波长的最小尺寸的孔或狭缝结构其填充孔或狭缝结构。本发明还提供了一种用于检测在这种发光传感器中存在于孔或狭缝结构中的一个或多个发光体产生的发光辐射的方法。

18. 发明申请

US20080272313A1 Luminescence Sensor Operating in Reflection Mode 有权
标题翻译：发光传感器在反射模式下工作
公开(公告)号：US20080272313A1
公开(公告)日：2008-11-06
申请号：US12158451
申请日：2006-12-19
申请人： Maarten Marinus Johannes Wilhemus Van Herpen , Derk Jan Wilfred Klunder , Hendrik Roelof Stapert
发明人： Maarten Marinus Johannes Wilhemus Van Herpen , Derk Jan Wilfred Klunder , Hendrik Roelof Stapert
IPC分类号： G01N21/64
CPC分类号： G01N21/648 , G01N21/6428
摘要： The present invention relates to a luminescence sensor (10), such as a luminescence biosensor or a luminescence chemical sensor, comprising at least one aperture (1) having a first lateral dimension larger than the diffraction limit of excitation radiation (3) in a medium the at least one aperture (1) is filled with and a second lateral dimension below the diffraction limit of excitation radiation (3) in a medium the at least one aperture (1) is filled with. The invention furthermore relates to a method for the detection of luminescence radiation (5), e.g. fluorescence radiation, generated by at least one optically variable molecule (2), e.g. fluorophore, in the at least one aperture (1). The excitation radiation (3) is preferably polarised to suppress the excitation radiation (3) in the apertures (1). The luminescence sensor (10) according to the present invention is able to detect relatively low concentrations of optically variable molecules (2), e.g. fluorophores.
摘要翻译：本发明涉及一种发光传感器（10），例如发光生物传感器或发光化学传感器，其包括至少一个孔（1），其具有比介质中的激发辐射（3）的衍射极限大的第一横向尺寸所述至少一个孔（1）在所述至少一个孔（1）填充的介质中填充有低于激发辐射（3）的衍射极限的第二横向尺寸。本发明还涉及用于检测发光辐射（5）的方法，例如，由至少一个光学可变分子（2）产生的荧光辐射，例如。在所述至少一个孔（1）中的荧光团。激发辐射（3）优选地被极化以抑制孔（1）中的激发辐射（3）。根据本发明的发光传感器（10）能够检测相对低浓度的光学可变分子（2），例如，荧光团

19. 发明申请

US20080245971A1 Biosensors with Improved Sensitivity 审中-公开
标题翻译：具有提高灵敏度的生物传感器
公开(公告)号：US20080245971A1
公开(公告)日：2008-10-09
申请号：US12088943
申请日：2006-09-27
申请人： Reinhold Wimberger-Friedl , Hendrik Roelof Stapert
发明人： Reinhold Wimberger-Friedl , Hendrik Roelof Stapert
IPC分类号： G01J1/58
CPC分类号： G01N21/6454 , B01L3/5027 , B01L3/502707 , B01L2300/0654 , B01L2300/0681 , B01L2300/0819 , B01L2300/0877 , G01N21/05 , G01N21/6428 , G01N2021/0346
摘要： A sensor is described for use with at least one optical detector (5), the sensor comprising a substrate (11) with optical outlets, a porous membrane (12) and micro fluidic channels (13) for conducting analyte fluid towards sensing locations (10) of the porous membrane (12). The sensing locations (10) are adapted for at least restraining light variable molecules (23) which bind to analytes to be determined. The optical output of the light variable molecules changes when they are in close proximity to a target molecule. The micro fluidic channels (13) are shaped to reflect light emitted from the sensing locations (10) towards the optical outlets and the substrate has diffracting optical elements (15) aligned with microfluidic channels to diffract light towards the optical outlets. The diffracting optical elements can be lenses.
摘要翻译：传感器被描述为与至少一个光学检测器（5）一起使用，传感器包括具有光学出口的基底（11），多孔膜（12）和微流体通道（13），用于将分析物流体传导到感测位置（10 ）多孔膜（12）。感测位置（10）适于至少限制结合要测定的待分析物的光可变分子（23）。光可变分子的光学输出在它们紧邻目标分子时变化。微流体通道（13）成形为将从感测位置（10）发射的光朝向光学出口反射，并且衬底具有与微流体通道对准的衍射光学元件（15），以将光朝向光学出口衍射。衍射光学元件可以是透镜。

20. 发明申请

US20110207630A1 METHOD FOR TESTING AND QUALITY CONTROLLING OF NUCLEIC ACIDS ON A SUPPORT 审中-公开
标题翻译：用于支持核酸的测试和质量控制的方法
公开(公告)号：US20110207630A1
公开(公告)日：2011-08-25
申请号：US13121195
申请日：2009-09-29
申请人： Anke Pierik , Hendrik Roelof Stapert
发明人： Anke Pierik , Hendrik Roelof Stapert
IPC分类号： C40B30/04 , C40B40/06 , C07H21/04
CPC分类号： C12Q1/6837 , C12Q2565/518 , C12Q2525/173
摘要： The present invention relates to a method for testing nucleic acids on a support, comprising the immobilization of one or more nucleic acids via crosslinking, wherein each of the immobilized nucleic acids includes a stretch of nucleotides of only one basetype, the provision of labeled oligonucleotides complementary to said stretch of nucleotides, and the determination of a value indicative for the condition of said nucleic acids. The present invention further relates to a kit for testing nucleic acids on a support comprising an array of nucleic acids immobilized on a solid support, wherein each of the immobilized nucleic acids includes a stretch of nucleotides of only one basetype and a labeled oligonucleotide complementary to said stretch of nucleotides. The invention additionally concerns the use of a labeled oligonucleotide complementary to a stretch of nucleotides of only one basetype for testing the condition of nucleic acids immobilized on a solid support.
摘要翻译：本发明涉及一种用于在载体上测试核酸的方法，其包括通过交联固定一种或多种核酸，其中每个固定化的核酸包括仅一种碱基类型的一段核苷酸，提供标记的寡核苷酸互补到所述核苷酸区段，以及确定指示所述核酸条件的值。本发明还涉及一种用于在载体上测试核酸的试剂盒，其包含固定在固体支持物上的核酸阵列，其中每个固定化核酸包括仅一种碱基类型的核苷酸片段和与所述核苷酸延伸。本发明还涉及使用与仅一种碱基类型的一段核苷酸互补的标记的寡核苷酸来测试固定在固体支持物上的核酸的状态。

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