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11. 发明授权

US5814521A Metal ion determination by sandwich aggregation assay 失效
标题翻译：夹心聚集法测定金属离子
公开(公告)号：US5814521A
公开(公告)日：1998-09-29
申请号：US703982
申请日：1996-08-28
申请人： Eddy Chapoteau , Jonathan Craine , Bronislaw P. Czech , Anand Kumar , Koon-wah Leong
发明人： Eddy Chapoteau , Jonathan Craine , Bronislaw P. Czech , Anand Kumar , Koon-wah Leong
IPC分类号： G01N31/00 , G01N21/77 , G01N21/82 , G01N33/543 , G01N33/84 , G01N33/20 , G01N35/546
CPC分类号： G01N21/82 , G01N33/54313 , G01N33/84 , G01N2800/04 , Y10S436/909
摘要： The present invention relates to a method for the determination of polyvalent metal ions using a sandwich aggregation assay. One or more chelating agents capable of forming at least 2:1 or higher ratio stoichiometry complexes with polyvalent metal ions are attached to a suitable carrier such as latex particles through covalent bonds or by non-covalent interactions, such as hydophobic interactions. Upon complexation with the metal ions, the carriers of the complexing agent aggregate, causing an increase in light absorbance or light scattering which is proportional to the concentration of metal ions in the test sample. The measurement of the light absorbance or light scattering by a suitable instrument, such as a spectrophotometer or nephelometer provides a means for measuring the concentration of metal ions.
摘要翻译：本发明涉及使用夹心聚集测定法测定多价金属离子的方法。能够与多价金属离子形成至少2:1或更高比例化学计量配合物的一种或多种螯合剂通过共价键或通过非共价相互作用例如疏水相互作用连接到合适的载体如胶乳颗粒上。当与金属离子络合时，络合剂的载体聚集，引起与测试样品中金属离子浓度成比例的吸光度或光散射的增加。通过合适的仪器（例如分光光度计或浊度计）测量吸光度或光散射提供了测量金属离子浓度的方法。

12. 发明授权

US5187103A Colorimetric method and reagent for the assay of lithium in a test sample 失效
标题翻译：在测试样品中用于测定锂的颜料方法和试剂
公开(公告)号：US5187103A
公开(公告)日：1993-02-16
申请号：US648073
申请日：1991-01-31
申请人： Bronislaw P. Czech , Eddy Chapoteau , Anand Kumar
发明人： Bronislaw P. Czech , Eddy Chapoteau , Anand Kumar
IPC分类号： C07D498/08 , C07D498/18 , G01N31/00 , G01N31/22 , G01N33/52 , G01N33/84
CPC分类号： C07D498/08 , G01N33/52 , G01N33/84
摘要： A chromogenic cryptand found to be especially selective in the determination of lithium in blood of the general formula (I): ##STR1## where: n=1 or 2,R.sub.1 and R.sub.2, same or different, are hydrogen, lower alkyl, lower alkenyl, or lower alkylidene; andQ is a chromogenic moiety capable of providing a detectable response upon complexation of said compound with lithium ion and has the structure: ##STR2## wherein: X is CH, C--OH or N; and Z is p-nitrophenylazo, 2,4-dinitrophenylazo, 2,4,6-trinitrophenylazo, p-nitrostyryl, p-benzoquinonemonoimino, bis-(4-dimethylaminophenyl) hydroxymethyl, 3-phenylisothiazolyl-5-azo, thiazolyl-5-azo; or isothiazolyl-5-azo.A reagent composition and synthesis of a preferred chromogenic cryptand are also disclosed.

13. 发明授权

US06323036B1 Reagents and methods for releasing and measuring lead ions from biological matrices 失效
标题翻译：用于释放和测量生物基质中铅离子的试剂和方法
公开(公告)号：US06323036B1
公开(公告)日：2001-11-27
申请号：US09085808
申请日：1998-05-27
申请人： Eddy Chapoteau , Bronislaw P. Czech , Jonathan Craine
发明人： Eddy Chapoteau , Bronislaw P. Czech , Jonathan Craine
IPC分类号： G01N3320
CPC分类号： G01N33/84 , G01N2800/40 , Y10T436/25375 , Y10T436/255
摘要： The present invention provides a reagent for extracting lead ions from a variety of biological matrices such as human whole blood that has been anticoagulated with either heparin or EDTA, and lyophilized human blood and bovine EDTA whole blood. The released lead is measured in situ with a calorimetric porphyrin reagent without further manipulation of the supernatant. The lead ion releasing reagent can be used in conjunction with a variety of assay formats for the determination of lead in various biological fluids.
摘要翻译：本发明提供了一种用于从各种生物基质例如人肝全血中提取铅离子的试剂，其已经用肝素或EDTA抗凝，以及冻干的人血和牛EDTA全血。释放的铅用量热卟啉试剂原位测量，无需进一步操纵上清液。铅离子释放试剂可以与各种测定形式结合使用，用于测定各种生物液体中的铅。

14. 发明授权

US5891736A Reagents and methods for releasing and measuring lead ions from biological matrices 失效
标题翻译：用于释放和测量生物基质中铅离子的试剂和方法
公开(公告)号：US5891736A
公开(公告)日：1999-04-06
申请号：US752869
申请日：1996-11-20
申请人： Eddy Chapoteau , Bronislaw P. Czech , Jonathan Craine
发明人： Eddy Chapoteau , Bronislaw P. Czech , Jonathan Craine
IPC分类号： G01N33/48 , G01N33/84 , G01N33/20 , G01N1/18
CPC分类号： G01N33/84 , G01N2800/40 , Y10T436/25375 , Y10T436/255
摘要： The present invention provides a reagent for extracting lead ions from a variety of biological matrices such as human whole blood that has been anticoagulated with either heparin or EDTA, and lyophilized human blood and bovine EDTA whole blood. The released lead is measured in situ with a colorimetric porphyrin reagent without further manipulation of the supernatant. The lead ion releasing reagent can be used in conjunction with a variety of assay formats for the determination of lead in various biological fluids.
摘要翻译：本发明提供了一种用于从各种生物基质例如人肝全血中提取铅离子的试剂，其已经用肝素或EDTA抗凝，以及冻干的人血和牛EDTA全血。用比色卟啉试剂原位测量释放的铅，无需进一步操纵上清液。铅离子释放试剂可以与各种测定形式结合使用，用于测定各种生物液体中的铅。

15. 发明授权

US5262330A Colorimetric methods and reagents for the assay of calcium in a test sample 失效
标题翻译：用于测试样品中钙的比色法和试剂
公开(公告)号：US5262330A
公开(公告)日：1993-11-16
申请号：US842860
申请日：1992-02-27
申请人： Eddy Chapoteau , Bronislaw P. Czech , Wolodymyr I. Zazulak
发明人： Eddy Chapoteau , Bronislaw P. Czech , Wolodymyr I. Zazulak
IPC分类号： G01N21/78 , C07C245/08 , G01N31/00 , G01N31/22 , G01N33/84 , C09B29/00 , G01N33/20
CPC分类号： G01N33/84 , C07C245/08 , G01N31/22 , G01N2800/04
摘要： A chromogenic compound found to be especially selective in the determination of calcium in blood of the general formula (I): ##STR1## where: R is hydrogen or lower alkyl;X is CH.sub.2, CH.sub.2 CH.sub.2, CH.dbd.CH, O, S, SO.sub.2, S--S, or NR;X is CH, C--OH or N;M is p-nitrophenylazo, 2,4-dinitrophenylazo, 2,4,6-trinitrophenylazo, p-nitrostyryl, p-benzoquinoneimino, bis-(4-dimethylaminophenyl) hydroxymethyl, 3-phenylisothiazolyl-5-azo, thiazolyl-5-azo, or isothiazolyl-5-azo;k is 1;l is 0 or 1; andn is 0 or 1.

16. 发明授权

US4810351A Carbonate selective membrane and electrode 失效
标题翻译：碳酸盐选择膜和电极
公开(公告)号：US4810351A
公开(公告)日：1989-03-07
申请号：US41391
申请日：1987-06-01
申请人： Eddy Chapoteau , W. James Scott
发明人： Eddy Chapoteau , W. James Scott
IPC分类号： G01N27/333 , G01N27/46
CPC分类号： G01N27/3335
摘要： The carbonate ion selective membrane of the invention comprises (a) a polymeric inert support material; (b) a plasticizer effective to solubilize the support material; (c) a quaternary ammonium ion; (d) an ionophore which is preferentially reactive with carbonate ions; and (e) a hydrophobic molecule effective to exclude ions other than carbonate ions. The addition of a hydrophobic molecule to the membrane formulation serves two purposes. First, it assists in the "binding" of the organic constituents to the matrix. Second, it repels charged species, i.e., anions and cations, from the solution phase, thereby improving selectivity to the carbonate species which enters the membrane via selective complexation with the ionophore. The quaternary ammonium ion is preferably a C.sub.7 to C.sub.9 quaternary ammonium ion. In this case, the plasticizer is selected to be one effective to maximize the binding of carbonate ions with the C.sub.7 C.sub.9 quaternary ammonium ions. The invention further provides a carbonate ion selective electrode comprising an internal reference element in electrochemical contact with the membrane described above.
摘要翻译：本发明的碳酸盐离子选择性膜包括（a）聚合惰性载体材料; （b）有效溶解载体材料的增塑剂; （c）季铵离子; （d）优先与碳酸根离子反应的离子载体; 和（e）有效排除碳酸根离子以外的离子的疏水性分子。向膜配方中加入疏水性分子有两个目的。首先，它有助于有机成分与基质的“结合”。其次，它从溶液相中排斥带电物质，即阴离子和阳离子，从而通过与离子载体的选择性络合来提高对进入膜的碳酸盐物质的选择性。季铵离子优选为C 7〜C 9季铵离子。在这种情况下，增塑剂被选择为使碳酸根离子与C 7 C 9季铵离子结合最大化的增塑剂。本发明还提供一种碳酸盐离子选择性电极，其包括与上述膜电化学接触的内部参考元件。

17. 发明授权

US08715942B2 Methods for the detection of glycated hemoglobin 有权
标题翻译：检测糖化血红蛋白的方法
公开(公告)号：US08715942B2
公开(公告)日：2014-05-06
申请号：US12743615
申请日：2008-11-18
申请人： Eddy Chapoteau , Richard Edwards , Chester Swirski , Wolodymyr Zazulak
发明人： Eddy Chapoteau , Richard Edwards , Chester Swirski , Wolodymyr Zazulak
IPC分类号： G01N33/53
CPC分类号： G01N33/53 , C12Q1/37 , G01N33/723
摘要： Provided herein are methods for detecting glycated hemoglobin in, for example, human whole blood, that are not affected by the presence of variation in amino acid sequence that can exist in hemoglobin β chains. The methods detect all glycated hemoglobin in a sample, regardless of the form of the hemoglobin that has been glycated, and thus detect glycated human Hemoglobin A, Hemoglobin S, and Hemoglobin C.
摘要翻译：本文提供了检测例如人全血中的糖化血红蛋白的方法，其不受血红蛋白和bgr中可能存在的氨基酸序列的变化的影响; 链条该方法检测样品中的所有糖化血红蛋白，不管糖化血红蛋白的形式如何，从而检测糖化血红蛋白A，血红蛋白S和血红蛋白C.

18. 发明申请

US20100273181A1 METHODS FOR THE DETECTION OF GLYCATED HEMOGLOBIN 有权
标题翻译：检测糖化血红蛋白的方法
公开(公告)号：US20100273181A1
公开(公告)日：2010-10-28
申请号：US12743615
申请日：2008-11-18
申请人： Eddy Chapoteau , Richard Edwards , Chester Swirski , Wolodymyr Zazulak
发明人： Eddy Chapoteau , Richard Edwards , Chester Swirski , Wolodymyr Zazulak
IPC分类号： C12Q1/37 , G01N33/53 , G01N33/546
CPC分类号： G01N33/53 , C12Q1/37 , G01N33/723
摘要： Particular aspects of the present invention relate to methods for detecting glycated hemoglobin in, for example, human whole blood, that are not affected by the presence of variation in amino acid sequence that can exist in hemoglobin β chains. The methods detect all glycated hemoglobin in a sample, regardless of the form of the hemoglobin that has been glycated, and thus detect glycated human Hemoglobin A, Hemoglobin S, and Hemoglobin C.
摘要翻译：本发明的具体方面涉及检测例如人全血中的糖化血红蛋白的方法，其不受可能存在于血红蛋白中的氨基酸序列的变化的影响; 链条该方法检测样品中的所有糖化血红蛋白，不管糖化血红蛋白的形式如何，从而检测糖化血红蛋白A，血红蛋白S和血红蛋白C.

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