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11. 发明授权

US09707554B2 Simplified device for nucleic acid amplification and method for using same 有权
公开(公告)号：US09707554B2
公开(公告)日：2017-07-18
申请号：US13395348
申请日：2010-09-17
申请人： Patrick Broyer , Laurent Drazek , Agnès Dupont Filliard , Michel Guy , Frédéric Pinston , Magaly Ponsard-Fillette , Thierry Kollaroczy
发明人： Patrick Broyer , Laurent Drazek , Agnès Dupont Filliard , Michel Guy , Frédéric Pinston , Magaly Ponsard-Fillette , Thierry Kollaroczy
IPC分类号： C12Q1/68 , B01L3/02 , B01L3/00 , B01L7/00
CPC分类号： B01L3/0217 , B01L3/5027 , B01L7/52 , B01L2200/0621 , B01L2200/16 , B01L2300/0864 , B01L2400/0478 , B01L2400/086
摘要： The present invention relates to a disposable device (100) for amplifying at least one target nucleic acid present in a liquid and biological sample of interest, which consists of a solid body (2), at least one fluid channel (3) connecting an inlet (4), via which all or part of the sample of interest can be drawn up and/or discharged, and an outlet (5), which is itself connected to a means for the drawing up/discharging of the said sample of interest, the fluid channel (3) further comprising from the inlet (4) to the outlet (5): a first compartment (8) containing all or part of the thermostable constituents, a means (15) for mixing the constituents with the sample of interest, a second compartment (9) containing all or part of the non-thermostable constituents, and in addition, at least one zone intended for heating the said sample of interest (6) mixed with the said amplification constituents in order to allow the amplification of the target nucleic acid. The invention also proposes an amplification method using such a device.The said invention has a preferred application in the field of medical diagnosis.

12. 发明申请

US20120171662A1 Simplified Device for Nucleic Acid Amplification and Method for Using Same 有权
标题翻译：用于核酸扩增的简化装置及其使用方法
公开(公告)号：US20120171662A1
公开(公告)日：2012-07-05
申请号：US13395348
申请日：2010-09-17
申请人： Patrick Broyer , Laurent Drazek , Agnès Dupont Filliard , Michel Guy , Frédéric Oinston , Magaly Ponsard-Fillette
发明人： Patrick Broyer , Laurent Drazek , Agnès Dupont Filliard , Michel Guy , Frédéric Oinston , Magaly Ponsard-Fillette
IPC分类号： C12Q1/70 , C12Q1/68 , C12P19/34 , C12M1/02 , C12M1/34
CPC分类号： B01L3/0217 , B01L3/5027 , B01L7/52 , B01L2200/0621 , B01L2200/16 , B01L2300/0864 , B01L2400/0478 , B01L2400/086
摘要： The present invention relates to a disposable device (100) for amplifying at least one target nucleic acid present in a liquid and biological sample of interest, which consists of a solid body (2), at least one fluid channel (3) connecting an inlet (4), via which all or part of the sample of interest can be drawn up and/or discharged, and an outlet (5), which is itself connected to a means for the drawing up/discharging of the said sample of interest, the fluid channel (3) further comprising from the inlet (4) to the outlet (5): a first compartment (8) containing all or part of the thermostable constituents, a means (15) for mixing the constituents with the sample of interest, a second compartment (9) containing all or part of the non-thermostable constituents, and in addition, at least one zone intended for heating the said sample of interest (6) mixed with the said amplification constituents in order to allow the amplification of the target nucleic acid. The invention also proposes an amplification method using such a device.The said invention has a preferred application in the field of medical diagnosis.
摘要翻译：本发明涉及用于扩增存在于液体中的至少一种靶核酸的一次性装置（100）和感兴趣的生物样品，其由固体（2），至少一个流体通道（3）（4），通过其可以将所有或部分的感兴趣样品抽出和/或排出;以及出口（5），其本身连接到所述感兴趣的样品的抽出/排出装置，流体通道（3）还包括从入口（4）到出口（5）：含有全部或部分热稳定组分的第一隔室（8），用于将组分与感兴趣的样品混合的装置（15），包含全部或部分非热稳定组分的第二隔室（9），另外还包括用于加热与所述扩增组分混合的所述感兴趣样品（6）的至少一个区域，以允许扩增靶核酸。本发明还提出了使用这种装置的放大方法。所述发明在医学诊断领域具有优选的应用。

13. 发明申请

US20110250680A1 AUTOMATED SYSTEM FOR THE LYSIS OF MICROORGANISMS PRESENT IN A SAMPLE, FOR EXTRACTION AND FOR PURIFICATION OF THE NUCLEIC ACIDS OF SAID MICROORGANISMS FOR PURPOSES OF ANALYSIS 有权
标题翻译：用于提取微生物的样品的自动化系统，用于提取和纯化用于分析用途的相关微生物的核酸
公开(公告)号：US20110250680A1
公开(公告)日：2011-10-13
申请号：US13129997
申请日：2009-12-09
申请人： Patrick Broyer , Agnes Dupontfilliard , Massimo Galdiero , Michel Guy , Hermanus Johannes Maria Kreuwel , Emiliano Maione , Emiel Gerebern Maria Verwimp
发明人： Patrick Broyer , Agnes Dupontfilliard , Massimo Galdiero , Michel Guy , Hermanus Johannes Maria Kreuwel , Emiliano Maione , Emiel Gerebern Maria Verwimp
IPC分类号： C12N5/07 , C07H1/06 , C12N1/06 , C12M1/04 , C12M1/00 , C12N1/00
CPC分类号： C12M47/10 , C12M47/06
摘要： The present invention relates to, among other things, a device for collecting airborne microorganisms, said device having: an air collecting module, comprising: i. an upper element having an air admission duct permitting entry of an air stream into said module, said duct being provided, at its base, with means for disturbance of the air stream, ii. a lower element having air evacuating means permitting the air stream created to exit and said upper and lower elements can be made integral with one another so that the air stream can be created within said air collecting module; a cartridge, of roughly cylindrical shape, having a microorganism retention zone, said retention zone having means for lysis of the microorganisms, said cartridge being positioned within said air collecting module.
摘要翻译：本发明尤其涉及一种用于收集空气微生物的装置，所述装置具有：空气收集模块，包括：i。上部元件具有允许空气流进入所述模块的进气管道，所述管道在其底部设置有用于干扰气流的装置，ii。下部元件具有允许产生的空气流出口的空气排出装置，并且所述上部和下部元件可以彼此成一体，使得可以在所述空气收集模块内产生气流; 大致圆筒形的筒，具有微生物保留区，所述保留区具有用于微生物裂解的装置，所述盒位于所述空气收集模块内。

14. 发明申请

US20060049046A1 Flexible biochip 审中-公开
标题翻译：柔性生物芯片
公开(公告)号：US20060049046A1
公开(公告)日：2006-03-09
申请号：US10520105
申请日：2003-07-18
申请人： Marc Cuzin , Michel Guy , Philippe Cleuziat
发明人： Marc Cuzin , Michel Guy , Philippe Cleuziat
IPC分类号： G01N33/487 , G01N27/26
CPC分类号： G01N33/5438 , H01R12/59
摘要： The invention concerns a sensor (1), in particular a biosensor, comprising an electrically or electronically insulating support (2), including at least one active surface (20), a plurality of electrically or electronically conductive electrodes (31,32), arranged on the active surface (2a) of the support in a predetermined operative arrangement, exposed, that is said electrodes can be jointly placed in contact with a common external medium, for example a liquid medium; a plurality of electric terminals (4) respectively corresponding to said electrodes (4), arranged on the active surface (2a, 2b) of the support, exposed, that is said terminals can be electrically or electronically connected outside, independently of one another; a plurality of electrically or electronically conductive strips (5), extending along one (2a) and/or the other (2d) of the support surfaces, connecting the plurality of electrodes (31,32) respectively to the plurality of terminals (4); an electrically or electronically insulating material layer (6), coating one (2a) and/or the other (2b) of the support surfaces, covering at least part of said strip conductors and exposing both the electrodes (31,32) and the terminals (5). The invention is characterized in that, in combination, the plurality of electrodes (4) is arranged in an end zone (1a) opposite another end zone wherein electric terminals (5) are assembled together and the support (2) comprises at least one flexible zone (1c), located between the two end zones.
摘要翻译：本发明涉及一种传感器（1），特别是一种包括电或电绝缘支架（2）的传感器（1），包括至少一个有源表面（20），多个电或电导电电极（31,32）以预定的操作布置在支撑件的活动表面（2a）上暴露，即所述电极可以共同放置成与公共外部介质例如液体介质接触; 分别对应于所述电极（4）的多个电端子（4）布置在所述端子的所述有源表面（2a，2b）上，即所述端子可以电连接或电连接到外部，而不是一个另一个; 多个电或电子导电条（5），其沿着一个（2a）和/或另一个（2d）的支撑表面延伸，将多个电极（31,32）分别连接到多个端子 4）; 电或电绝缘材料层（6），涂覆一个（2a）和/或另一个（2b）的支撑表面，覆盖所述条状导体的至少一部分并暴露两个电极（31,32）和端子（5）。本发明的特征在于，组合地，多个电极（4）被布置在与其中端子（5）组装在一起的另一端部区域的端部区域（1a）中，并且支撑件（2）包括至少一个柔性区域（1c），位于两个端部区域之间。

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